Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,124	C→T	W481* (TGG→TAG)	cpdB ←	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,124	0	C	T	100.0%	94.5 / NA	32	W481* (TGG→TAG)	cpdB	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

CAAAAGCGATATGGCTGTCGCCCGTACCGGCAAATTTGCCGCCGTAAGCGCGATAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCGGCAGCTGAAAGCACCGGCAGT  >  NC_000913/4434882‑4435369                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      cAAAAGCGATATGGCTGTAGCCCGTACAGGCCCATTTGCCGCGGTAAGCGAGATAGTTATTGGTGGCACCCAGGAAAATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGATCTTAATACTTTCCGCATTGGCATTAATCATCTGGCAATCGCCGTCATAACGGGCGGGCTGGGTAACAGCAATCTGATAATTACCACCATCAATAACATCAAAGTTATAAGTGCGGAAACCAACCTAGTtgatg                                                                                                                                                                                                                                               >  1:407165/1‑251 (MQ=255)                   cGCCCGTCCCGGCAAATTTGCCGCCGTAAGCGCGATAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAAGCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTGTCGt                                                                                                                                                                                                                              <  1:168674/250‑1 (MQ=255)                      ccGTTCCGGCAAGTTGGCCTCCGTAAGCGCGATATTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCGTAATCCTTTCCGCATTGGCATTAATCATCTGTCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTTTTAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGAGGAGTGACTGTGGTTTCGTGCTg                                                                                                                                                                                                                          <  1:296706/251‑1 (MQ=255)                           ccGGCAAATTTGCCGCCGTAAGCGCGATAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCTTCTGGCACTCGCCGTCATAACGGGCGGGCTGGGAAACATCAATCTGATCATTCACACCATCAATAACTTCAAAGATATAGGTACGGAAACCATCCTAGTTGATAAGGGCCTGTGTTTCCGTGCTGTTggg                                                                                                                                                                                                                     >  2:107412/1‑251 (MQ=255)                             ggCAAATTTGCCGCCGTAAGCGCGATAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGAt                                                                                                                                                                                                                   <  1:111315/251‑1 (MQ=255)                                            gTAAGCGCGATAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACt                                                                                                                                                                                                    <  1:294081/251‑1 (MQ=255)                                              aaGCGCGATAGTTATTGGTGGCACCCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGTATCAATCTGGTTAAACTGt                                                                                                                                                                                                  <  1:151298/251‑1 (MQ=255)                                                 cgcgATAGTTATTGGTGGAAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACAATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGTTCAATCTGGTTAAACTGTccc                                                                                                                                                                                               >  1:352971/1‑251 (MQ=255)                                                     aTAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTGCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAAGCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAATCATCCTAGTtg                                                                                                                                                                                                                                                  <  2:92876/196‑1 (MQ=255)                                                           aTTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGTATCAATCTGGTTAAACTGTCCCGCGGAGCACt                                                                                                                                                                                     <  1:334774/251‑1 (MQ=255)                                                                    aaCCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCAct                                                                                                                                                                            <  1:26796/251‑1 (MQ=255)                                                                              aTGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACctc                                                                                                                                                                  >  1:167609/1‑251 (MQ=255)                                                                                                         ttAAAGGACAGGTTCTTAATCCTATCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCCGGCTGGGTAACCTCAATCTGATAATTCACACCATCAATAACATCCAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTTCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGAACTCCCGCCCCTCTTTCACCTCTTTCCCGCTGGCTTTCACCACAATctg                                                                                                                                       >  2:284047/1‑249 (MQ=255)                                                                                                              ggTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAAGCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCCCTCTTTCCCCTCTTTACCGCTGGCTTTCACCCCAATCAGCGTAt                                                                                                                                  <  1:107412/251‑1 (MQ=255)                                                                                                               gtaaGGTTCTTAACCCTTTCCGCATTGGCCTTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGATCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTAtt                                                                                                                                 <  2:101679/248‑1 (MQ=255)                                                                                                                  aGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTggg                                                                                                                              >  2:251931/1‑251 (MQ=255)                                                                                                                         tAATCCTTGCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATaga                                                                                                                         <  2:334231/249‑1 (MQ=255)                                                                                                                               tttCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGCGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATaa                                                                                                                    >  1:363635/1‑248 (MQ=255)                                                                                                                                                   atcTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGaatgt                                                                                             >  1:466463/1‑248 (MQ=255)                                                                                                                                                                   cATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTcc                                                                             >  1:18443/1‑251 (MQ=255)                                                                                                                                                                    agaaCGGGCGGGCTGGTTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTATTTTATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTGCACCTCTTTACCGCTGGCGTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGATGGTCAACTGGCCTTTTTCCa                                                                            <  2:87263/249‑1 (MQ=255)                                                                                                                                                                                       aCATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCAATCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCCCAATCAGCGTATTGGGATAGCGATAAAGATCGGCGGCATTACGGAAGGTCAAATGGCCTTTTTCCACCTCCACATAGCTTGCCgg                                                         >  2:416658/1‑251 (MQ=255)                                                                                                                                                                                                    aaTTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCg                                             <  1:14956/250‑1 (MQ=255)                                                                                                                                                                                                        cacaCCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGacca                                         <  1:312469/250‑1 (MQ=255)                                                                                                                                                                                                                cAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCCACGACttt                                 >  2:247093/1‑250 (MQ=255)                                                                                                                                                                                                                          aaaGTTATAAGTGCGGAAAACATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCgg                      >  1:350051/1‑251 (MQ=255)                                                                                                                                                                                                                                ataAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCGGCAGCt                 <  1:127898/250‑1 (MQ=255)                                                                                                                                                                                                                                    gTGCGAAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGATGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTAACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAAATGGCCTTTTTCCACCTCCACATAGCTTGCCGGGGCAGTTTTGCGACCACCGCCTTTAACCGGAGCGGCAGCTGaacg            >  2:313458/1‑249 (MQ=255)                                                                                                                                                                                                                                      gCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCTCTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTGGCCGGGGCATTTTTGCGACCACCGACTTTAAACGGTGCGGCAGCTGAAAGc           <  1:169821/250‑1 (MQ=255)                                                                                                                                                                                                                                         gAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTTTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACGCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCGGCAGCTGAAAGCACCg       <  1:282006/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaCCATCCTAGTTGAGGATTGACTGTGGTTTCGTGCTGTGGGGATCAATCTGGTTAAACTGCCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCGTGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCTACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCGCCAGCTGAAAGCACCGGc     <  2:24088/251‑1 (MQ=255)                                                                                                                                                                                                                                              cATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCGGCAGCTGAAAGCACCGGCAGt  >  1:357597/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      CAAAAGCGATATGGCTGTCGCCCGTACCGGCAAATTTGCCGCCGTAAGCGCGATAGTTATTGGTGGCAACCAGGAACATGGCGTTCGGATCAATCGGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATTACGGAAGGTCAACTGGCCTTTTTCCACCTCCACATAGCTTGCCGGGTCATTTTTGCGACCACCGACTTTAAACGGTGCGGCAGCTGAAAGCACCGGCAGT  >  NC_000913/4434882‑4435369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑