Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,426,929 C→T C165Y (TGC→TAC)  yjfZ ← DUF2686 domain‑containing protein YjfZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,426,9290CT95.8% 65.7 / NA 24C165Y (TGC→TAC) yjfZDUF2686 domain‑containing protein YjfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/14);  minor base G (0/1);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

ATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATC  >  NC_000913/4426697‑4427163
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
atacatacGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAg                                                                                                                                                                                                                          <  2:38313/251‑1 (MQ=255)
      acGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAgg                                                                                                                                                                                                                     <  1:326198/250‑1 (MQ=255)
       cGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGt                                                                                                                                                                                                                   >  2:456766/1‑251 (MQ=255)
         aGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGtt                                                                                                                                                                                                                  >  1:75958/1‑250 (MQ=255)
                  gaaggctcGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGTGGCTGAACTTGATAAGGTACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAAGACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGTCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAAGTAGGCATT‑TGGGCAGGAAGGGTTACTACGTATc                                                                                                                                                                                                        <  2:446471/246‑1 (MQ=255)
                  gATGTCTCGCTCCTGCGTCACTCGCTCGAAACCCAAACCCTAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAAGGCAGCAATGATGAATCCGAGGGCATTGTAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCATATGACCAGTAATTAGGCATT‑TGGGCATGAAGGGTTACAACGTATc                                                                                                                                                                                                        <  1:416357/251‑1 (MQ=255)
                   atttctcGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGTAGACCAAATTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATTAATCCGATGTCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGGTCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATcc                                                                                                                                                                                                       <  1:451438/248‑1 (MQ=255)
                                                          gaggaGATTGCGGCTGGACTTGATAAGGAACGCAGACCAATTTTCAAATCAGGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGTGTTACAACGTATCCTAGCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAgg                                                                                                                                                                <  2:315036/247‑1 (MQ=255)
                                                                                              ccAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGtt                                                                                                                            <  1:456766/251‑1 (MQ=255)
                                                                                                     tCAAATTATGGGGTCAATCAGAATACGCGTTTAGCAATGTAGCAATGATGATTCCGAGGGAATTAGAAACGCCTGTAACAGTGCGACGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCATATCGTTTGGCCGGGATAGTCTGGAGTACCATAACCAGGTCCCAAAACTACTTCGTGTATTGTGTATTTACTGTTGGGTTCg                                                                                                                     >  2:200365/1‑251 (MQ=255)
                                                                                                      cggaTCATGGGGTAAATCAGAATACGCGTGTAGCAATGGAGCAAGGATGAGTCCGATGGCAGTAGAGTCGGCTGTAACAGTGCTAAGTTGGACTATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGGAATTAGGCATTGTGGG‑AGTAAGGGTTACAACGTATCCTATCGGTTGGCTGGTATAGTCTGGAGTTCCATAACAAGGTCCCAAATCCTCTTCGTGTATTCTGTATTTACTGTTGGGTTCg                                                                                                                     <  1:177028/247‑1 (MQ=255)
                                                                                                                                             agcaATGATGAATCAGAGGGCATTAGAAACGCCTGTAACAGAGCGAAGTTGGTCGATATAACGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACAAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCgg                                                                             >  2:376769/1‑251 (MQ=255)
                                                                                                                                               caATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGTGTTCGAAGGATTCGGGATCTTGCAATAAATATTTCAGTTCTGCGGtt                                                                           <  1:389452/251‑1 (MQ=255)
                                                                                                                                                   gatgaATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAAGTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTGCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAAc                                                                        <  1:225208/250‑1 (MQ=255)
                                                                                                                                                                     aGAAACGCCTGTAACAGTTCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCtt                                                     <  1:83536/251‑1 (MQ=255)
                                                                                                                                                                      gAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGATCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCttt                                                    >  2:133001/1‑251 (MQ=255)
                                                                                                                                                                       aaaCGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTa                                                   <  1:322457/251‑1 (MQ=255)
                                                                                                                                                                               gTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTAt                                             <  2:107822/249‑1 (MQ=255)
                                                                                                                                                                                    aGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTggg                                      >  1:293002/1‑251 (MQ=255)
                                                                                                                                                                                     gTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGc                                     <  1:42116/251‑1 (MQ=255)
                                                                                                                                                                                            gTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAat                               >  2:248730/1‑250 (MQ=255)
                                                                                                                                                                                                                  tgttgGAAGTTCAGATGACCAGTAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGt        >  2:153152/1‑251 (MQ=255)
                                                                                                                                                                                                                    ttgGTAGTTAAGATGCACAGTAAATAGGCATT‑TGGGCCGGACGGGTTACATCGTATCCTCTCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCATGCAATAAATATTTAAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTATGGGCGGGCATATTATCTAATGTAGCTTCATGGTtc      >  2:307836/1‑251 (MQ=255)
                                                                                                                                                                                                                         aGTTCAGATGACCAGTAATTAGGCATT‑TGGGCATGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACTATAACCAGGTACCAAAACCATTTCGTGTATTGTGTATTTACTGTTGGGTTCTAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTtccata  <  2:150147/250‑2 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
ATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCTCGAAACCCAAACCCGAGTAGATTGCGGCTGAACTTGATAAGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATT‑TGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTCAGTTCTGCGGTTAACCTCGCACCCGCGTAAGCTTTATTGTATTTCTGGGCGTGAATATTATCTAATGTAGCTTCATGGTTCCATC  >  NC_000913/4426697‑4427163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑