| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,403,010 | C→T | R325C (CGT→TGT) | hflK → | regulator of FtsH protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,403,010 | 0 | C | T | 98.6% | 220.6 / NA | 69 | R325C (CGT→TGT) | hflK | regulator of FtsH protease |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (33/35); minor base A (1/0); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGAAGAAGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGC > NC_000913/4402767‑4403255 | ccgGAAGAAGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTCCTCGCGCGTGTCTGt > 2:375060/1‑250 (MQ=255) gaagaaGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGACGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTatat > 1:20313/1‑251 (MQ=255) gaagaaGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTatat > 2:156523/1‑251 (MQ=255) gaagaaGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTatat < 1:254250/251‑1 (MQ=255) gaagaaGCAAAAGAGGCGTTTGACGAAGCGCTTGTCGCGCGTGAAAACGACCAGCAATACATTCGTGCAGCAGAAGCGTATAACAACGAAGTTCAGCCGCGTGCGAACGGTAAGGCGCAACGAATCATCGAAGAGGCGCGGGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTAAGGGTGAAGTGGCGCGCTTTGCTCAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAATGTCTGTatat > 2:73144/1‑251 (MQ=255) gaagTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCga < 1:190430/251‑1 (MQ=255) aaaaGCGGCGGTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACg < 1:266283/250‑1 (MQ=255) aaaGCGGCGTTTGACGATGCGAGTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGGGGCGCGCTTTGCGAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACGCGCGAGTGTCTGTATATCGAGACGaa < 2:313255/251‑2 (MQ=255) cggcgTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGa > 2:111240/1‑250 (MQ=255) ggcgTTTGACGATGCGATTGCCGCTCGTGAAAACGAACAGCAAGACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTGTCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGAGGCGTGCTTTGCTAAACGTCTGCCGTAATATAAAGCCGCGCCGGGAAGTGCTCGCTAGTGTCTGTATATCGAGACTATGGaaa < 2:305993/251‑1 (MQ=255) ggcgTTTGACGATGCGATTGCCGCGCGTGAAAACGAACCGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCCGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGaa > 2:222110/1‑250 (MQ=255) ttGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTatat < 2:53254/232‑1 (MQ=255) ttGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTatat > 1:53254/1‑232 (MQ=255) ttGACGAGGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAgt < 2:398886/250‑1 (MQ=255) gCGATTGCCGCGCATGAAAACGAACAGCAATATATTCGTGAAGCAGAAGCGGATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGATGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGTAATATAAGGCCGCGCCGGAGATTACTCTCGAGTGTCTGTGTATCGAGACGAGTGAAAAATTGTTGGG‑Taa < 2:73662/251‑1 (MQ=255) gATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAac > 1:150713/1‑250 (MQ=255) aTTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAacac > 1:119707/1‑251 (MQ=255) gcctcgcgTAAAAACTAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCTTGCGAACGTTCAGGCTCAACGTTTCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGTAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAACCCGCGCCGGAAATTACGCGCGAGTTTCTGTATCTCGCGACGATTCAAAAAGTGTTGGT‑TTACACCCg < 2:411639/247‑1 (MQ=255) ccGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGc > 1:253404/1‑251 (MQ=255) cgTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGTGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGAGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGt < 2:315403/251‑1 (MQ=255) aaCAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGCGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGCATATAAAGCCGAGCCGGAAATTACTCGCGAGTGTCTGTATCTCGAGACGATGGAAAAAGTGTTGGG‑TCACACCCGCAAAGTGCTGGTTAAc > 1:461215/1‑251 (MQ=255) atcAATACCTTCGTTAAGCAGAAGCGTATTCCAACGAAGGTCTGCCGCGTGCGAACGGTCAGGCTTAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCAAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCGAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGAt < 2:350233/249‑1 (MQ=255) gcaatgccttCGTGAAGCAGAAGCGTATACCACCGCAGTTCAGCCGCGTGCGAACGGTCAGTCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAACCTTCTGCCGGACTATCAAGCAGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGGAAAAGGGATGGG‑TAACCACCGCAAAGTGTTGGttcacact > 2:269553/9‑244 (MQ=255) cAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGTGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATa > 2:164613/1‑250 (MQ=255) aCATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAgg < 2:20313/250‑1 (MQ=255) cATTCGTGAAGCAGAAGAGTATACAAACACAGGTCAGCCGCGTGCGACCGGTCAGGAGCAACGTATCCTCGAAGAGGCGCCTGCGTCCCAGGAC‑AAACCAATCCTGGGAGCTCAGGGCGAAGTGGCGCGCTTTGCCAAACATCTGACGCAATATAACGCCGCGCCGGGAATTACTCGCGCGAGTCTGTAGATAGAGACGCTGGAAGAGGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAgatg > 2:38319/1‑248 (MQ=255) gacgCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGACAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATCTCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCt > 2:37990/4‑251 (MQ=255) cAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGa < 1:157378/249‑1 (MQ=255) cAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGAAACCTGa > 2:157378/1‑249 (MQ=255) aaGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGtt > 1:294391/1‑251 (MQ=255) cGTATACCAACGAAGTTCAGCCGCGTGCGAATGGTCAGGCGCAACGGATCCTCGAAGAGGCGTGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGAAGATGGAAAATGTGTTGGG‑TAACACCCTCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTg < 2:401485/251‑1 (MQ=255) cGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAgtgt > 1:111001/1‑195 (MQ=255) cGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAgtgt < 2:111001/195‑1 (MQ=255) cccaCGAAGTTCAGCCGCGTTCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAGCCTGATGGTTCTGCCGTTa < 1:3240/248‑1 (MQ=255) agAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCGCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTACACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGAcc < 1:451135/250‑1 (MQ=255) gAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGAcc > 1:37905/1‑250 (MQ=255) ttCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCACAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATg > 1:408405/1‑251 (MQ=255) cAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGc < 1:374016/250‑1 (MQ=255) gCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGa < 2:325716/251‑1 (MQ=255) gAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTa < 2:345181/251‑1 (MQ=255) gAACGGTCAGGCGCAACGTATCCTCGAAGAAGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGAGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAggtggt > 2:59879/1‑250 (MQ=255) ggTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGGGTACAATGCCCAGACCA‑TCCTGGAATCTCAGGGTGAAGTTGCGCGCTTTGCTAAACGTCTGCCGGAACATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGGTACGATGGAAAAAG‑GTTGGGCTAACACCCGCAAAGGGCTGGTTGACGATAAAGCTGGCAGCCTGATGGTTCTGCCGTTAGACCACATGCGGAAAGGTGGTAACGc < 2:450101/251‑1 (MQ=255) cAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGccc < 2:119707/250‑1 (MQ=255) aCGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTgcggcg > 1:173722/1‑250 (MQ=255) ccTCGAAGAGGCGCGTGCGTACAAGGCCCCGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCg > 1:139576/1‑251 (MQ=255) ccTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGc < 1:156523/250‑1 (MQ=255) cgcgTGCGTGCAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACGTCTGCCGGAATATAAAGCCGCGCCTGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTTCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGcc < 1:260151/251‑1 (MQ=255) cgcgTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGcc < 2:173722/251‑1 (MQ=255) cgtACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAAt > 1:111502/1‑251 (MQ=255) aCAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATctg > 1:240381/1‑251 (MQ=255) aaagtcccAGACCA‑TCCTGGAAGCTCAGGGTGAGGTGGCGTGCTTTGCTAAACTTCTGCCGGAATATAAATCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGAGGGAAAAAGTGTTGGT‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTTGTAACGCCCCTGCGGCGAAGAGCTATAACGGTGCCAGCAATctgc < 2:327711/246‑1 (MQ=255) tCCTGGAAGCTCAGGGTGAAGTAGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTgccgcc > 1:342886/1‑250 (MQ=255) aGCTCAGGGTGAAGGGGCGCGCTTTGCTAAACATCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGGGTTGGG‑TAACACCCGCAACGTGCTGGTTAACGATAAAGGTGGCACCCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTTGTAACGCCCCTGCGGCGAAGAGCGATAACGGTTCCATCAATCTGCTGCGTCTACCCCCAGCctcgt < 2:214872/251‑3 (MQ=255) gcgcTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGag < 1:93341/251‑1 (MQ=255) cgcTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGGTAGACCAGATGCTGAAAGGGGGTAACGCCCCTGCGGCGAAGTGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGagc < 2:176876/251‑1 (MQ=255) tttGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGagca > 2:330186/1‑249 (MQ=255) tCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACAcgtcg < 1:330186/250‑1 (MQ=255) tCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACAcgtc < 1:246505/249‑1 (MQ=255) cTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACgtcgtc > 2:316711/1‑251 (MQ=255) aaaGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGCGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGCGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAggg > 2:232658/1‑251 (MQ=255) aaaGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAACAGTGTTGGG‑TAACACCCGCAAAGAGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAggg > 2:224162/1‑251 (MQ=255) aaaGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAggg > 1:78532/1‑251 (MQ=255) gCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCgg > 1:88348/1‑250 (MQ=255) cgcgCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGCAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGACGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGat > 1:58593/1‑250 (MQ=255) ccGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtattat < 2:37905/251‑1 (MQ=255) aTTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCa < 2:240381/251‑1 (MQ=255) aTTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCa < 1:222110/251‑1 (MQ=255) cgcgAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTAGGGACCAAcgc < 2:294391/249‑1 (MQ=255) tcgAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAAcgccgc > 2:65791/2‑251 (MQ=255) | CCGGAAGAAGTAAAAGCGGCGTTTGACGATGCGATTGCCGCGCGTGAAAACGAACAGCAATACATTCGTGAAGCAGAAGCGTATACCAACGAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCA‑TCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGG‑TAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGC > NC_000913/4402767‑4403255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |