| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,401,607 | C→T | T312T (ACC→ACT) | hflX → | ribosome rescue factor HflX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,401,607 | 0 | C | T | 98.7% | 235.7 / NA | 80 | T312T (ACC→ACT) | hflX | ribosome rescue factor HflX |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (37/41); minor base G (0/1); total (38/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
CCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACCCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGG > NC_000913/4401371‑4401850 | ccGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTTCTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGTTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGaa < 2:11958/251‑1 (MQ=255)ccGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGtgatga > 1:134069/1‑250 (MQ=255) cGACGTTCCGGCTTATTTCCGTTGCAAATGTTTGTGAATCCTGACTTCCAGATACCGTAGGGTTTATTCGCCTCCTGCCGCACGATCTGGTGGCGGCATTGAAATCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAATAAAACATCGAAGCGGTGAATTCGGTTCTTGAAGAG‑ATCGACGCTCACGAGAGCCCAACTCTGC‑TGGTGATGAAc < 2:210913/251‑1 (MQ=255) aCGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAAATCTGC‑TGGTGATGAACa > 2:146595/1‑250 (MQ=255) aCGTTGCGGCGTATTGACGATGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGGTGAACa > 1:94845/1‑250 (MQ=255) gTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGGGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCCACTCTGC‑TGGTGATGAACa > 2:142605/1‑248 (MQ=255) ggTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGatat < 2:320789/250‑1 (MQ=255) cgTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGGTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGatat > 1:31651/1‑251 (MQ=255) cgTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTACACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TTGTGATGAACAAGATCGatat < 2:233682/251‑1 (MQ=255) gTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATg < 1:154226/251‑1 (MQ=255) ttGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGGGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGa > 1:124789/1‑247 (MQ=255) gTCGGTGAAACCGTACTTGCAGATCCCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAc < 1:383470/250‑1 (MQ=255) gTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAc < 1:185619/250‑1 (MQ=255) tCGGTGAAACAGTACCTGCAGATACAGTAGGCTTTATTCGCAACCTGACGCCCGCTCTGGTAGCGGCATTTAAAGACACGTTACAAGAGGCGCGGCAAGCCACCTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACCAGAAACCATCGAAGAGGAGAATCAGAATCTTGGAGAA‑ATCGCCGCTCACGAGCTCCCAACTCTGC‑TGGGGGTGAACAAAGTCGAAATGGTGGAAGGTTTTGaaacg > 2:418707/1‑248 (MQ=255) gAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAt < 1:438885/251‑1 (MQ=255) cGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGGACCGCGTATTgctt > 1:176132/1‑248 (MQ=255) cGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATc < 1:68699/251‑1 (MQ=255) cGTACTGGCAGATACCGTAGGGTTTATTCGCAACCTGCCGCACGATCCGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCTCGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATc < 1:221160/251‑1 (MQ=255) ttGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAAACCTGC‑TGGTGATGAAAAAGATCGATATGCTGGAAGATTTCGAACCGCGTCTTGATCGGGAc > 2:421236/1‑251 (MQ=255) tGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACGCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGAc < 2:265595/250‑1 (MQ=255) aGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGa > 1:142390/1‑249 (MQ=255) gATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAaga < 2:9249/251‑1 (MQ=255) ccGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAACGACACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATAGACGGCAACGAGCTCCCAACTCTGC‑TGGTGATGAAAAAGATAGATATGATGGAAGATTTCGAACCGCGGTTTGTTCGGGGTGAAGAGaa > 1:69334/1‑250 (MQ=255) ccGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGCCGCCGCAAGCCACCTTACTGCTGCACGTCATTGATGCGGCGGAAGTGCGGGTACAAGAAAACATCGAAGTGGAGAATACGGTTCTTGAACAGGAT‑GCCCCTCACGAGATCCCAACTATTC‑TGGTGCTGTACAAGCTGGATTTGCATGTAGATTTCGAACCGCGTATTGTCCTGGACGACGAGAAc > 1:421707/1‑251 (MQ=255) aGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGGACAAGAAAACATCGAAGCGGGGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGGTCCCCACTCCGC‑TGGGGATGCACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGGCGAAGAGAACaaa > 2:147511/1‑250 (MQ=255) ggTTTATTCGCCTCCTGCCGCACGAGCTGGTGGCGTCATTTAAAGCCACGTGACAAGGGTCGCGGCAAGCCACATTACTGCTGCACGGCATTGATTCGGCGTAGGTGCGTGTACAAGAAAACATCGAAGGGGTGAATTCGGGTCTTGAAGAG‑ATCGACGCTCACGATTTCCCAACTCTGC‑TGGGGATGAACAAGATCGAGAGGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg < 1:32531/251‑1 (MQ=255) aTTCGCCCCCTGCCGCACGAGCGGGTGGCGGCATTTAAAGCCACGTGTCAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTTTTGATCGGGACGAAGATAACAaaccgaacc < 2:446703/250‑1 (MQ=255) cGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgtgt < 1:155533/251‑1 (MQ=255) cGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGAGGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgtg < 1:403450/250‑1 (MQ=255) cACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGa < 2:110070/188‑1 (MQ=255) cACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGa > 1:110070/1‑188 (MQ=255) cACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGGGCGTGTACAAGAAAACAACGAAGCGGTGAATACAGTTCTTGAAGCG‑ATCGACGCTCAAGAGATCCCAACTCTGC‑TGGTGATGAACAAAATCGATATGCTGGAAAATTTCGAACCGCGTATTGATCGGGACGAAAAGAACCAAACGAACCgtgtttg > 2:332558/1‑248 (MQ=255) aCGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGGGTACAAGAAAACATCGAAGCGGAGAATACGGTTCTTTAAGAG‑ATCGACGCTCACGAGATCCCCACTCTGCGTGGT‑ATGAACAAGATCGATATGCTTGAAGATTTCGAACCGCGTATTGATCGGGGCGATGAGAACAAACCGAACCGTGTCGTGCTTTCCGcc > 2:360039/1‑250 (MQ=255) aTCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGGCTGGCTTTCCGcac > 2:129630/1‑249 (MQ=255) tCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGAGTACAAGAAAAAATCGAAGCGGTGGATACGGTTCTTGAAGAG‑ATCGAAGCTCCCGAGAACCCCCCTCTGC‑TGGTGAAGAACAAGAACGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGGAGAGAACAAACCGAACCGTGTTTGGGGTTCCGcac > 2:268056/1‑248 (MQ=255) tggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGCAGAG‑ATAGACGCTCACGAGATCCCAACTCTGA‑TGGAGATGAACCAGATCGATATGCTGGAAGCTTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGAcc > 1:231459/1‑251 (MQ=255) tggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGAcc > 1:49707/1‑251 (MQ=255) ggtggCGGTATTAAAAGTCACGTTACATGATACGCGGCAAGCCGCATTACTGCTGCAAGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCATCTCTGC‑TTGTGATGAACAAGATCTATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACTAAGAGAACAAACCGAACCGTGTCTGGCTTTCTGCACAGAcc < 1:265998/250‑1 (MQ=255) ggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAATATCGATATGCTGGAAGATTTCGAACCGCGTATTGAGCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGAcc < 1:47501/250‑1 (MQ=255) gggggcggcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAg < 1:120898/244‑1 (MQ=255) tttAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGGGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgggg > 2:7382/1‑249 (MQ=255) aaaGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATAcc < 2:16599/251‑1 (MQ=255) tACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAAACGAACCCGGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttttt > 1:108023/1‑251 (MQ=255) cAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCGCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCGGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCa < 1:158779/251‑1 (MQ=255) gagaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCg < 1:466482/223‑1 (MQ=255) agaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACCGACCGGAGCGGGGATACCACAGCTTTTTCAGGCt > 1:55924/1‑251 (MQ=255) aCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGCTACCACAGCTTTTTCAGGCttt > 1:109295/1‑251 (MQ=255) aCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCttt < 2:341909/251‑1 (MQ=255) gCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGCGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAACAG‑ATCGCCGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGCTATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACCGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACgg > 1:303622/1‑251 (MQ=255) aaGCCACATTCCTGCTGCACGTCATTGATGCGGCGGAGGTGCGTGTACAAGAAAACATCGAAGCGGGGAATAAGGGTCGTGAAGAG‑GTCGATGCGCACGAGATCCCAACTCTGA‑TGGTGATGAACAAGATCGGTATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAAAAAACCGAACCGGGACTGGCTTTCCGCACAGACCTGAGAGGGGATACCACAGCTTTTTCGGGCTTTGTCGGa > 2:249487/1‑250 (MQ=255) gCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCg < 1:277886/251‑1 (MQ=255) aTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCttt < 2:80998/251‑1 (MQ=255) aTTACTGCTGCACGTCAGTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCttt < 2:188616/251‑1 (MQ=255) gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCgg < 1:421236/249‑1 (MQ=255) gTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGGAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGTCGGAGCGGCTTTCCGGCGAgggggg > 2:395602/1‑247 (MQ=255) cATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGgcg < 1:170675/250‑1 (MQ=255) aTTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCgca < 1:142605/251‑1 (MQ=255) gcggcgGATGGGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATa < 2:31651/250‑1 (MQ=255) gtgCGTGTACACGAAAACATCGAAGCGGTGAATACGGTTCTTGAGGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCAGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACGGACCGGAGCGGGGCTACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCCGCATACATTGCGTCt > 2:36934/1‑251 (MQ=255) gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCt < 1:205519/251‑1 (MQ=255) gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTGCCGGCGAGGTGGCGCAGCATACATTGCGTCt < 1:257052/251‑1 (MQ=255) gtgtACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGcc > 1:342484/1‑250 (MQ=255) tACAAGAACACATCGAAGCGGCGACTACGGTTCTTGAAGAG‑ATCGCCGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGAc > 2:85106/1‑202 (MQ=255) aaaaCATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGa > 1:401797/1‑250 (MQ=255) aaCATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAgg < 1:138003/251‑1 (MQ=255) aCATCGAAGCGGTGAATCCGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGCTGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGCACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGAAACCACAGCTTTTTCCGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCCCCGCAGGAAggg > 2:389681/1‑251 (MQ=255) aacGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGTAGCGGCTGGCCGGCGATGTGGCGCAGCATACATTGCTTCTGCCACCGCAGGAAGGGCg < 2:69334/249‑1 (MQ=255) cGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTc < 1:299279/251‑1 (MQ=255) gCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCTCCGCacctacggcgtctgag < 1:147511/251‑16 (MQ=255) cGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATCTGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGACCAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTg > 1:295770/1‑248 (MQ=255) gAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTGTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCTTCTGAGAAGTc < 2:105350/251‑1 (MQ=255) aaTACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGaa < 2:24658/110‑1 (MQ=255) aaTACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGaa > 1:24658/1‑110 (MQ=255) aCGGTTCTGGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTTATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGttt < 2:50801/251‑1 (MQ=255) cGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGtttt > 2:139691/1‑251 (MQ=255) tGAAGAG‑ATCGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAg < 1:146595/250‑1 (MQ=255) aaGAG‑ATCGACGCTCACGAGAGCACAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCtt < 2:183183/251‑1 (MQ=255) cGACGCTCACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGCAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCa > 2:341245/1‑250 (MQ=255) cACGAGATCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaaa > 1:369930/1‑250 (MQ=255) tCCCAACTCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGCACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAACGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTATGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCACTAGAAATagagggg > 1:457007/1‑248 (MQ=255) | CCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTTTATTCGCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAG‑ATCGACGCTCACGAGATCCCAACCCTGC‑TGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGG > NC_000913/4401371‑4401850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |