Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,388,120 C→T A417A (GCG→GCA)  mscM ← miniconductance mechanosensitive channel MscM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,1200CT98.8% 272.8 / NA 85A417A (GCG→GCA) mscMminiconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (41/43);  minor base G (0/1);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATC  >  NC_000913/4387873‑4388362
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
gAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTTACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGTGCGATTTCCAGCGGCCAGGCTATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCa                                                                                                                                                                                                                                                 <  1:444914/251‑1 (MQ=255)
 aaaaCGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAt                                                                                                                                                                                                                                                >  1:4036/1‑251 (MQ=255)
      gggTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCGTTCAGTGCAtcctcc                                                                                                                                                                                                                                           <  1:67067/251‑1 (MQ=255)
       ggTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAACCAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGACATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAtcctcc                                                                                                                                                                                                                                           >  1:39221/1‑250 (MQ=255)
              tAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCCTCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGccc                                                                                                                                                                                                                                   >  1:170425/1‑251 (MQ=255)
               aGCGGCGCGAGTAAATCCTGCAACCGACAAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCCGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCg                                                                                                                                                                                                                                  >  1:57473/1‑251 (MQ=255)
                  ggcgCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGCTTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTg                                                                                                                                                                                                                               >  2:165351/1‑251 (MQ=255)
                          tAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGAGGCCAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATCGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACttt                                                                                                                                                                                                                       >  2:431186/1‑251 (MQ=255)
                                cTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCtt                                                                                                                                                                                                                 >  1:153221/1‑251 (MQ=255)
                                  gCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTg                                                                                                                                                                                                                <  1:5833/250‑1 (MQ=255)
                                        gACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACTCTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGCCCAGAACAGATAGCGGTGCGTTGCTTCGTTCTCCTCTTTCAGTGCATCCTCCAGTTTCCCGTTGGAGACTTTCAGCTTGGTCAGtt                                                                                                                                                                                                         <  1:244959/251‑1 (MQ=255)
                                        gACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGtt                                                                                                                                                                                                         >  1:147925/1‑251 (MQ=255)
                                        gACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGtt                                                                                                                                                                                                         >  1:392580/1‑251 (MQ=255)
                                           cAGAATCAACGCGCCAAACCGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCTATTTCCAGCGTCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCTGTGCGTTGCTTCGTTCTCCTCTGTCAGTGCATCCTCCAGTGGCCCGTTCGAGACTTTCAGCTCGTTTAGTTCCa                                                                                                                                                                                                      <  2:70161/251‑1 (MQ=255)
                                               aTCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCATTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGTTAGCGGTGCGTTGCTGCGTTCACCACTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAAtag                                                                                                                                                                                                  <  2:402012/251‑1 (MQ=255)
                                                       gcCAAACAGCGGCAAAATTTTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGAGTCCAGCGAAATGAGACGACGCAGATCATGGGCGAGTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGGACAGATAGCGGTGCGTTGCTTCGTTAAACTCTTTCAGTGCATTCTCACGTTGCCAGTTGGAGACTTTCATCTTGGTCAGTTCCAATAGTAGGgtgt                                                                                                                                                                                          >  2:70539/1‑251 (MQ=255)
                                                            aCAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGTTCAGTTCCAATAGTAGCGTGTCGcc                                                                                                                                                                                      >  2:413320/1‑250 (MQ=255)
                                                                cGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCt                                                                                                                                                                                 >  1:5902/1‑251 (MQ=255)
                                                                     aaaTTGTCTCTTTGCTGGTCAGCATCATTACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGAGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGcaac                                                                                                                                                                            >  2:41527/1‑251 (MQ=255)
                                                                             tctTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAcc                                                                                                                                                                                   >  1:325891/1‑236 (MQ=255)
                                                                             tctTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGGTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAcc                                                                                                                                                                                   <  2:325891/236‑1 (MQ=255)
                                                                               tttGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTc                                                                                                                                                                   >  1:97994/1‑250 (MQ=255)
                                                                                 tGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGc                                                                                                                                                                >  2:294878/1‑251 (MQ=255)
                                                                                      gTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATTAGTTCAGCAACTc                                                                                                                                                           >  2:232995/1‑251 (MQ=255)
                                                                                        cAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCa                                                                                                                                                          <  2:147925/250‑1 (MQ=255)
                                                                                        cAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCAc                                                                                                                                                         >  1:338565/1‑251 (MQ=255)
                                                                                         aGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGAGTCCAGCGACATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCCCGTCAGAGGTCCAGCACAGATAGCGGCGCGTTGCTTCGTTCACCTCTTTCAGTTCATCCTCCAGTTGCCCGCTGGGAACTTTCAGCTTGGTCAGTTCCAATAGTCGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACg                                                                                                                                                        >  2:3326/1‑251 (MQ=255)
                                                                                          gCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGc                                                                                                                                                       >  1:335780/1‑251 (MQ=255)
                                                                                              catcaCCCTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAAGAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAg                                                                                                                                                   <  1:37195/251‑1 (MQ=255)
                                                                                              catcaCACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGa                                                                                                                                                    <  2:5902/250‑1 (MQ=255)
                                                                                                tcaCACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTg                                                                                                                                                 >  1:248037/1‑251 (MQ=255)
                                                                                                       ggCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATGTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGtt                                                                                                                                          <  1:310547/251‑1 (MQ=255)
                                                                                                           ttGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgtg                                                                                                                                       <  2:392580/250‑1 (MQ=255)
                                                                                                            tGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCt                                                                                                                                     >  1:1286/1‑251 (MQ=255)
                                                                                                              cccAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTc                                                                                                                                   <  1:184967/251‑1 (MQ=255)
                                                                                                                 aagtgactgaGGGGGCCCAGCGAAATGCGACGACGCAGGTCCTGGGCCATTTCCAGCGGCCTGACGTGGGTCATCGGGCGCACGCCAGAGGTCCAGAACAGTTAGCGGTGCGTTGCTTCGTTCACCTCTTTCATGGCAACCTCCAGGTGCCCGGTGTAGACTTTCAGCTTGGTCGTTTCCAATAGTGGCGTGTCGCAACCCTGCAACAATGAGTGCAGCAACGCACGCTGAGATCGCGGTtgtgatgcgag                                                                                                                                <  2:323221/248‑8 (MQ=255)
                                                                                                                 aaCTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAg                                                                                                                                <  1:250616/251‑1 (MQ=255)
                                                                                                                     gactgaAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAAt                                                                                                                             <  2:335780/250‑1 (MQ=255)
                                                                                                                         gaAGGTGTCCAGCGAAATGAGACGACGCAGATCCGGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTGGGAGACTTTCAGCTTGGTCAGTGCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACg                                                                                                                          <  2:195234/249‑1 (MQ=255)
                                                                                                                          aaGGTGTCCAGCGAAAGGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAAGGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGtt                                                                                                                       <  2:62828/251‑1 (MQ=255)
                                                                                                                           aGGTGTCCAGCGAAATGAGACGACGCAGTTCCTGGGCGATTACAAGCGGCCAGGCGATGTGCATCGGGCGCGCGACAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTTGTTCTCCTCTTGCAGTGCATCCTCCAGTTGCCCGTTGGAGTCTATCAGCTTGGTCATTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTGCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATTCGGttt                                                                                                                      <  2:203511/251‑1 (MQ=255)
                                                                                                                           aGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGttt                                                                                                                      >  1:356342/1‑251 (MQ=255)
                                                                                                                             gtgtACAGCGAAATGAGACGCCGCCGATCCTGGGCGATTTCCAGCGGCAAGGCGATGGACATCGGGCGCACGTCAGCGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTg                                                                                                                    >  2:281645/1‑251 (MQ=255)
                                                                                                                               gtCCAGCGAAATGAGACGACGCAGCTCATGGGCGATTTACAGCGGCAAGGCGATGGTCATCGGGAGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCCATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTAACGCTGGGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCt                                                                                                                  >  2:448927/1‑251 (MQ=255)
                                                                                                                                     cGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAg                                                                                                            <  1:139586/251‑1 (MQ=255)
                                                                                                                                         aTGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTc                                                                                                          >  1:4913/1‑249 (MQ=255)
                                                                                                                                          ggaggcgacgCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGGGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAGCCTCCAGTTGCCCGTTGGAGACTGTCAGCTTGGTCAGTGCCAAGAGTAGCGTGGCGCCAGCGTGCAACAAGGAGGTCATGAACGCACGCTGAGTGCGCAGTGGTGCTTCCAGAATTCGGGTTTGCGGGGCAGTCAGc                                                                                                       <  2:192136/246‑1 (MQ=255)
                                                                                                                                              acgacgCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCt                                                                                                   <  1:384631/251‑1 (MQ=255)
                                                                                                                                                   gCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCg                                                                                              >  1:379093/1‑251 (MQ=255)
                                                                                                                                                     aGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCCGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTc                                                                                            >  2:4253/1‑251 (MQ=255)
                                                                                                                                                     aGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTc                                                                                            >  1:26361/1‑251 (MQ=255)
                                                                                                                                                      gATCCTTGGCGATTTCCAGAGGCCAAACAATGGTCATCGGGCGCACCTCAGAGCTCCAGCACAGATAGCGGTGCGTTGTTTCGATCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCGGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGCTCAGCAACTCCCGCTGAGTGCGCAATTGCGCTTCCAGAATACGGTTTTGCTCGGAAGTCAGCGGCTGAcgttcg                                                                                           >  2:448162/1‑246 (MQ=255)
                                                                                                                                                      gATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTGTCAGTGCATCCTCCAGTTGCCTGTTGGAGACTTTCAGCTTGGGCAGTTCCAATAGTAGCGTGTCGCCTCCCTGCAACAATGAGTGCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCg                                                                                           <  1:66/251‑1 (MQ=255)
                                                                                                                                                           tGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCtg                                                                                      >  1:131307/1‑251 (MQ=255)
                                                                                                                                                               cGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCtgatga                                                                                  <  1:4253/251‑1 (MQ=255)
                                                                                                                                                                gATTTCCAGCGGCCAGGCGATGGTCATCGGGCTCACGTCAGAGGTCCAGAACAGATAGCTGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAAGACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGaa                                                                                 <  1:23784/251‑1 (MQ=255)
                                                                                                                                                                     ccAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGc                                                                            <  1:228611/251‑1 (MQ=255)
                                                                                                                                                                            ccAGGCGATGGTCGTCGGGCGCACGTCAGAGGTCCAGAACAGAGAGCGGTGCGTTGCGTCGTTCACCTCTTTCAGTGCAGCCTCCAGTTGCCCGTTGGAGACTTTCAGCTGGGTCAGTTCCAAGAGTAGCGGGTCGCCACCGTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTATTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAAGTTGCCGTagca                                                                     <  2:387250/251‑1 (MQ=255)
                                                                                                                                                                                  gATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGTTGCGTTGCTGCGTTCATCTCTTTCAGTGCATCCGCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGGTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGGGCGCAGTTGTGCGTCCAGAAGACGGTTTTGCGCGGCAGTCAGCGGCTGACCGTCGGCCGGATGAATTTGCCGTAGCAGCGGCt                                                               <  2:46895/251‑1 (MQ=255)
                                                                                                                                                                                   aTGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTg                                                              >  1:436063/1‑251 (MQ=255)
                                                                                                                                                                                      gTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCt                                                                                                                                                      <  2:47770/160‑1 (MQ=255)
                                                                                                                                                                                      gTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCt                                                                                                                                                      >  1:47770/1‑160 (MQ=255)
                                                                                                                                                                                         aTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTttatt                                                        >  1:74966/1‑251 (MQ=255)
                                                                                                                                                                                                  aCGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATc                                               <  1:325808/251‑1 (MQ=255)
                                                                                                                                                                                                   cGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCTCGCTGAGTGCGCACTTGTTCTTCCAGAAt                                                                                                                             <  2:188176/172‑1 (MQ=255)
                                                                                                                                                                                                   cGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAAt                                                                                                                             >  1:188176/1‑172 (MQ=255)
                                                                                                                                                                                                   cGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGTTTTTGCTCGGCAGTCAGCGGCTGACCGTCTGTCTGATGAATTTGCCGTAGCAGCTGCTGTTTATTAAGTAGATcc                                              <  2:307272/251‑1 (MQ=255)
                                                                                                                                                                                                      cAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCa                                           <  1:165351/251‑1 (MQ=255)
                                                                                                                                                                                                       agagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCAt                                          <  2:138515/251‑1 (MQ=255)
                                                                                                                                                                                                       agagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCAt                                          >  1:98592/1‑251 (MQ=255)
                                                                                                                                                                                                          ggTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAAc                                       <  2:131307/251‑1 (MQ=255)
                                                                                                                                                                                                           gTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCATTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACTCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGGTTAGTAAGCAGAGCCTCATAACg                                      <  2:310781/251‑1 (MQ=255)
                                                                                                                                                                                                           gTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACg                                      >  1:162917/1‑251 (MQ=255)
                                                                                                                                                                                                             ccAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTa                                    <  1:147040/251‑1 (MQ=255)
                                                                                                                                                                                                                gAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAAcc                                 >  1:65250/1‑251 (MQ=255)
                                                                                                                                                                                                                    aGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTg                             <  1:99752/251‑1 (MQ=255)
                                                                                                                                                                                                                     gATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCGCCAGTTGCCCGTTGGAGACTGTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCCCCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGc                            <  2:354967/251‑1 (MQ=255)
                                                                                                                                                                                                                            gtgggttgttTCGTTTACCTCTGTTAGTGCATCCCCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCGGCAACAATGAGTTCAGCAACGCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCa                     <  1:244548/247‑1 (MQ=255)
                                                                                                                                                                                                                               cGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCACTAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACt                  >  1:225409/1‑251 (MQ=255)
                                                                                                                                                                                                                                  tGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTgc                <  1:429229/250‑1 (MQ=255)
                                                                                                                                                                                                                                           ccccTCTTTCAGTGCATCCTCCAGTTGCTCGTTTGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCg       <  2:208733/248‑1 (MQ=255)
                                                                                                                                                                                                                                           cACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCg       <  1:152106/250‑1 (MQ=255)
                                                                                                                                                                                                                                               tctTTCAGTGCATCCTCCAGATGCCCGTTGGAGATTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGCCGCCACCCTCCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTCACCGTTTCACACGCCACTGCGCCATTTCGGTATc  >  2:294469/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ttCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCg                                                                                              <  1:75601/156‑1 (MQ=255)
                                                                                                                                                                                                                                                  ttCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGACACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCCGTCAGCGGCTGACCg                                                                                              >  2:75601/1‑156 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
GAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATCAACGCGCCAAACAGCGGCAAAATTGTCTCTTTGCTGGTCAGCATCATCACACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATC  >  NC_000913/4387873‑4388362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑