Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,421	C→T	A272T (GCG→ACG)	yjeJ ←	protein YjeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,421	0	C	T	100.0%	144.7 / NA	45	A272T (GCG→ACG)	yjeJ	protein YjeJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

GCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGTT  >  NC_000913/4373175‑4373667                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGTCCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAgcgtgcgt                                                                                                                                                                                                                                                    <  1:207307/251‑1 (MQ=255)     ggTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGc                                                                                                                                                                                                                                                <  2:257886/251‑1 (MQ=255)      gTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAACAACGATCACGTTATCAGTCATTTCATGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGc                                                                                                                                                                                                                                                >  1:453952/1‑250 (MQ=255)       tGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGCTGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAt                                                                                                                                                                                                                                              >  2:396314/1‑251 (MQ=255)           aaaCGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGAt                                                                                                                                                                                                                                          >  1:328170/1‑251 (MQ=255)                   aaTTGCATGAGGCGCTTCGCTTAACCGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTGATCAGTCATTTCAGGCTGGTGGGCTCCAGCCCCGCATTAGCCAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACAAGAAAGTGATGCAGCGTGCGTCAGCATTGATCCTGGGtt                                                                                                                                                                                                                                  >  2:396335/1‑251 (MQ=255)                    aTTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTa                                                                                                                                                                                                                                 <  2:22294/251‑1 (MQ=255)                     ttGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGtt                                                                                                                                                                                                                                  >  1:191149/1‑249 (MQ=255)                                 cttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTgg                                                                                                                                                                                                                         <  2:83108/246‑1 (MQ=255)                                 cttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTgg                                                                                                                                                                                                                         >  1:83108/1‑246 (MQ=255)                                    cgcttCTCAGGACTACATAAGGCATTGCAACTGAATGAATGCTTGAGAATTTGTAGCCCGGATAAGGCGTTTACGCCGCATCAGGCATGACTACCGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGCGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTAtt                                                                                                                                                                                                                 >  2:303305/1‑251 (MQ=255)                                        tATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgc                                                                                                                                                                                                              <  2:418399/250‑1 (MQ=255)                                                    aTAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCg                                                                                                                                                                                                  >  1:270814/1‑250 (MQ=255)                                                      aaGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAAc                                                                                                                                                                                               >  2:162720/1‑251 (MQ=255)                                                                    gagtGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGGTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACAt                                                                                                                                                                                  <  1:396314/247‑1 (MQ=255)                                                                            gCTTGAGACTATATAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATAACGTTATCAGTCCTTTCAGGCTGGACGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTAAGCGGTTGGGCGTTGTTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACAccc                                                                                                                                                                         >  2:57235/1‑251 (MQ=255)                                                                             cTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTGAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCcg                                                                                                                                                                        >  2:106500/1‑251 (MQ=255)                                                                                         tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGCCCAACGCTCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTc                                                                                                                                                             >  2:98826/1‑250 (MQ=255)                                                                                          ggtccGGATTAGGCGGTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCATCCTCGCATTAGCAAAGATGAGATTATTGCGCCTGTGGTGCAGTGTTGCGGGTGGATTGAACACGTAAGTTATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAg                                                                                                                                                           <  2:327402/248‑1 (MQ=255)                                                                                                            aCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCaa                                                                                                                                          <  1:61323/250‑1 (MQ=255)                                                                                                                   aTCCGGCATGTCCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGTTCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCgg                                                                                                                                  <  1:40247/251‑1 (MQ=255)                                                                                                                        gCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGt                                                                                                                             >  1:223964/1‑251 (MQ=255)                                                                                                                                       aCGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTc                                                                                                              >  1:69602/1‑251 (MQ=255)                                                                                                                                         gTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACTGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCtt                                                                                                            <  1:178017/251‑1 (MQ=255)                                                                                                                                                          gCTGGATGGTGCCAGTCTCGTATTAGCAAAGATGAGATTATTTCGCCCGTTGTGCAGTTTTGCTGGTGGATTGAACTCGTAAGCGATGCAGTGTGCCTAAGCTTTGATCCTGGGTTATCGGTTGGTCGTTAGTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGTGAGTTTTTTCAGTCTTGGGCTGAAATCAAGCTTTCGGCGAGTGATCGCTTCCATTTCTTTACCCGTCGTTGCTTCa                                                                                           <  2:261701/251‑1 (MQ=255)                                                                                                                                                                        gCCTCGCATTAGCAAAGATGAGATTATTGCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAATTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCGTTACCCGGCGTTGCTGCACGCGTCTTcaccac                                                                             <  2:44449/251‑1 (MQ=255)                                                                                                                                                                           tCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTACGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTgc                                                                          >  1:309469/1‑251 (MQ=255)                                                                                                                                                                                ttAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGcgccgc                                                                     >  1:167198/1‑251 (MQ=255)                                                                                                                                                                                          tGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTAcc                                                                                                        <  1:260365/206‑1 (MQ=255)                                                                                                                                                                                          tGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTAcc                                                                                                        >  2:260365/1‑206 (MQ=255)                                                                                                                                                                                            agaTTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGGGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGGCCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCATCACTGCGCCTCTAAATTGTTCtg                                                         <  2:330311/251‑2 (MQ=255)                                                                                                                                                                                            agaTTATTTAGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCtt                                                         >  2:442606/1‑251 (MQ=255)                                                                                                                                                                                                tattTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGcc                                                     >  1:402809/1‑251 (MQ=255)                                                                                                                                                                                                     cGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACtt                                                 <  1:96393/250‑1 (MQ=255)                                                                                                                                                                                                            ggtgCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCttt                                         <  1:228852/251‑1 (MQ=255)                                                                                                                                                                                                                       gCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTTGTCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAAGCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTaa                               <  2:328170/250‑1 (MQ=255)                                                                                                                                                                                                                       gCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTaa                               >  1:188408/1‑250 (MQ=255)                                                                                                                                                                                                                             ggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCg                        >  1:144408/1‑251 (MQ=255)                                                                                                                                                                                                                                  gAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGcc                      >  2:110402/1‑248 (MQ=255)                                                                                                                                                                                                                                  gAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGGCGTGGGCTGAAATCAAGCAGTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGTTTTTCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAg                    <  1:57235/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaCACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGAt                  <  1:258179/251‑1 (MQ=255)                                                                                                                                                                                                                                     cacGTACGTGATGCAGCGTGCGCCACCATTGATCATGGGTTAGCGGTTGGGCGTTATTCGCCGCTCCGGTGCGAACATAGACCTGACATGGCACAACCGCGCGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGAGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAACTTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATa                 >  2:193590/1‑250 (MQ=255)                                                                                                                                                                                                                                        gTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTGAAAGCGGTAAAGCACCGCCAGATAATgg             <  2:213202/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGTGATGAAGCGTGCCTAAGCATTGATACTGGGTTAGCGGTTGGGCGTTATTCGACGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAACTCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCg          >  2:310132/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gCAGCGTGAGTAAGAATTGATCCTGGGTTAGCGGTTGGGCGTTAATCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCTACTTTCATATTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACacg    >  2:437248/1‑249 (MQ=255)                                                                                                                                                                                                                                                   aGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGtt  >  1:367712/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGTT  >  NC_000913/4373175‑4373667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑