Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,349,259 C→T intergenic (‑515/+56) dcuB ← / ← dcuR anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,349,2590CT97.6% 137.5 / ‑6.4 43intergenic (‑515/+56)dcuB/dcuRanaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/13);  minor base G (1/0);  total (29/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTG  >  NC_000913/4349015‑4349496
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
tGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAggg                                                                                                                                                                                                                                            <  2:318057/250‑1 (MQ=255)
                      tGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTaataat                                                                                                                                                                                                                     >  1:465892/1‑251 (MQ=255)
                                              gTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGAt                                                                                                                                                                                             >  2:9947/1‑251 (MQ=255)
                                                   aaGATTAATCTTATCTACCCAATTGTTGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCGGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGAGCTGGCACAAGTTTTACTGATTAATGATTTAACTTGTGACAGGGGTATTTTGCAGTACGGTAATAATTATTACTTACAGATAACCTGCGTTCCGtt                                                                                                                                                                                        <  2:61743/250‑1 (MQ=255)
                                                      aTTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTAtt                                                                                                                                                                                     <  1:408312/251‑1 (MQ=255)
                                                      aTTAATCTGATCTACCCATTTGTGAGTAAAACTACACATAATGCGGGTGACATAATAGTTAATTACCTTTTGTTAGCGTTTTGCAATTAAAAACACCGTTCACCTAAAGAGATATTAATTTTTAGCGATGATGGAGGGATCATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCGGGGGAATTTTGCATTACGTTAATAATTATTACTTACAGATAACCAGCGATCCGTTAtt                                                                                                                                                                                     >  2:265097/1‑251 (MQ=255)
                                                                 cTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGt                                                                                                                                                                          >  2:346669/1‑251 (MQ=255)
                                                                             gggTAAAACTACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAACTTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATCTTGTTTCagtcgtg                                                                                                                                                                >  2:223655/1‑249 (MQ=255)
                                                                                              aTGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTACAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATCATATTTGATCTGACACAAGTTTTACTGATGAAGGATGTAACTTGCGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTAAAGATAACCAGCGCTCCGTTATTGACAATATTGTTTCAGTAGAGTGTAGTGTTCTGCCTGAAt                                                                                                                                             >  1:448101/1‑251 (MQ=255)
                                                                                                       aCATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGCTAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATacggtaacg                                                                                                                                    >  2:217625/1‑251 (MQ=255)
                                                                                                         ataataGTTAATTAACTTTTGTTAGAGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATacggtaacggt                                                                                                                                  >  2:453874/1‑251 (MQ=255)
                                                                                                                                     tttGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATg                                                                                                      >  1:457947/1‑251 (MQ=255)
                                                                                                                                      ttGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTAtttt                                                                                                                                                                                                                                     >  1:85689/1‑123 (MQ=255)
                                                                                                                                      ttGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTAtttt                                                                                                                                                                                                                                     <  2:85689/123‑1 (MQ=255)
                                                                                                                                           aTTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTg                                                                                                                                                <  1:266535/203‑1 (MQ=255)
                                                                                                                                           aTTAAAAACACCGTTCAACTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTg                                                                                                                                                >  2:266535/1‑203 (MQ=255)
                                                                                                                                              aaaaaCACCGGTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAAGATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGCCGCCCCGTGACGCCATAATGGATACTGGt                                                                                             <  1:203235/251‑1 (MQ=255)
                                                                                                                                                            ccTGAAGAGGTATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTACCGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGc                                                                              >  1:24716/1‑251 (MQ=255)
                                                                                                                                                             cTGAAGAGATATTACTTTTTAGCGATGATGGAGGGATAATTATCTTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGTCGCCATAATGGATACTGGTGAACA‑AGAtgtgtc                                                                              >  2:57317/1‑248 (MQ=255)
                                                                                                                                                                     atatTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGAcca                                                                     <  1:262271/251‑1 (MQ=255)
                                                                                                                                                                     atatTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTGTCAGTAGTGAGTAGTGTTCTGACTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGGGGCAGTGGAcca                                                                     <  2:395380/251‑1 (MQ=255)
                                                                                                                                                                               ttAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATc                                                                                                                                                                                            <  1:32739/123‑1 (MQ=255)
                                                                                                                                                                               ttAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATc                                                                                                                                                                                            >  2:32739/1‑123 (MQ=255)
                                                                                                                                                                                tAGCGATGATGGAGGGGTAATTATCTTTGATCTGGCACAAGTCTTCCTGATGAAGGGAGTAAATTGCGTAAGAGGTATTTTGCATTACGGTAATAATTATTACTTACTGCTAACCAGCGATCCGTGATTGGCAATATTGTTTCAGTAGTGCGTAGTGTTCTGCCTGAATAGGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGA‑CACAGAAGTGGCTGTTGAcactcccgatgagg                                                          >  2:214236/1‑238 (MQ=255)
                                                                                                                                                                                   cGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGCAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTAt                                                       >  2:314612/1‑251 (MQ=255)
                                                                                                                                                                                    gatgatGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGTTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTAtt                                                      <  1:91899/251‑1 (MQ=255)
                                                                                                                                                                                       gatgGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTAc                                                   >  1:160207/1‑251 (MQ=255)
                                                                                                                                                                                              ggATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGa                                            <  2:465892/251‑1 (MQ=255)
                                                                                                                                                                                               ggtAATTCTATTTGATCGGGCACAAGTTTTACTGATGAAGGCTGTAACTTGTTTCAGGGGAATTTTGCATTACGGTAATAATTATTACATACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGAGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGACCCGTGACTCACTAATGTATACTGGTGAACA‑AGCTGTGGCAGTTGACCAGCCCGCTGAGGTATTTACGGCaagc                                            >  2:92343/3‑247 (MQ=255)
                                                                                                                                                                                                  aaTTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAac                                         >  2:219674/1‑250 (MQ=255)
                                                                                                                                                                                                   aTTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTAAAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGAGGCAGTTGACCAGCCAGATGAGGGATTTACTGCaagcaaaa                                        >  2:56680/1‑243 (MQ=255)
                                                                                                                                                                                                      atatTTGATCTGGCACAAGTTTTGCTGATGAAGGCTCTACCTTGAGTCAGGGGTATTTTGAATTACGGTAATAATTATTCCTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGCGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACC‑AGATGTGGCAGTTGCCCAGCCAGATGAGGTATTTACGGCAGGAAACAc‑gcg                                   >  2:291247/1‑251 (MQ=255)
                                                                                                                                                                                                        atTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGa                                  >  1:201367/1‑250 (MQ=255)
                                                                                                                                                                                                         tttGATCTGGCACAAGTTTTACTGATGACGGATGTAACTTGTGTCAGGAGAATTTTGCATTACGGGAATCATTATTACTTCCAGATAACCAGCGATCAGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCAGTGACGCCCTAATGGATACTGGTGAACA‑AGATGTGGCAGTTGAACAGACAAATGAGGGATTTACGGAAGGAAacaaaccaa                                  >  2:368448/1‑244 (MQ=255)
                                                                                                                                                                                                             aTCTGGCCCAAGTTTTACTGATGACGGCTGTAACTTCTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAAACAGAGATCCGTTATTGGCAATATTGTTTCAGTAGCGAGTCGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGCACA‑AGATGTGGCAGTTGACCAGCCAGCTGAGGTCTTTACGGAAGGAA‑CACCGCGAAATGtt                            >  2:23087/1‑251 (MQ=255)
                                                                                                                                                                                                             aTCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGAACTGtt                            >  2:445687/1‑251 (MQ=255)
                                                                                                                                                                                                                    acaAGTTTTACTGATGAAGGATGTAACTTGTGTCAGTGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCTGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGAGGAGGTATTTACGGCAGGAAACAC‑GAGAAATGTTAACCTCg                     <  2:364030/251‑1 (MQ=255)
                                                                                                                                                                                                                      aaGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGaa                                                                                          <  2:400344/182‑1 (MQ=255)
                                                                                                                                                                                                                      aaGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGaa                                                                                          >  1:400344/1‑182 (MQ=255)
                                                                                                                                                                                                                             aCTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGAAATGTTAACCTCGTTGGCTAGc            <  1:314612/251‑1 (MQ=255)
                                                                                                                                                                                                                              cTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGAAATGTTAACCTCGTTGGCTAGCt           >  1:101331/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aGGATGTCACTTGTGGCAGGGGAATTTTGCATTCCGGTAATAATTATTAATTACAGATACCCAGCGATCCGTTATTGGCCATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGCACA‑AGATGTGGCAGTTGCCCAGCCAGATGAGGTATTTACGGCAGGAAAACC‑GCGAAATGTTAACCCCGCTGGCTAGCTCGTCggg    >  2:238652/1‑250 (MQ=255)
                                                                                                                                                                                                                                       gATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGAAGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGGTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGAAATGTTAACCTCGTTGGCTAGCTCGACgggtg  >  1:218257/1‑248 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
TGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACA‑AGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACAC‑GCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTG  >  NC_000913/4349015‑4349496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑