Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,501	C→T	L371L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,501	0	C	T	98.9%	293.1 / NA	93	L371L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (57/35);  minor base A (1/0);  total (58/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCG  >  NC_000913/23254‑23747                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTgg                                                                                                                                                                                                                                                     <  1:302417/251‑1 (MQ=255)     tACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACTGCACTTATCTGCCGGGCACTTATCCGACGTTGGATg                                                                                                                                                                                                                                                  <  2:271140/250‑1 (MQ=255)                 ggCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCtt                                                                                                                                                                                                                                     >  1:145247/1‑251 (MQ=255)                   cACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACACATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGCCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGGACGTCTTc                                                                                                                                                                                                                                    >  2:45868/1‑250 (MQ=255)                    aCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGAGGGCGTGAACGTCTTCa                                                                                                                                                                                                                                   >  1:304403/1‑250 (MQ=255)                      ggTAAAAGGTGCGGAGCTTGCGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCaa                                                                                                                                                                                                                                  >  1:394830/1‑249 (MQ=255)                              gTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGAc                                                                                                                                                                                                                        <  2:278586/251‑1 (MQ=255)                                gCGGAGCTTGAGCGGAGGCGCTTTCCCCATCCGTTTATGGGCTTCGACGGTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTCCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTTGAAACCGCGAACCCGGTTGGCCCGGACGGCACTGATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt                                                                                                                                                                                                                      <  2:394830/251‑1 (MQ=255)                                        tGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGTCACCTAGCTGCCGGGCACTTATCCGACGTTGGATGGCGTTGACGTCTGCAAAGCGAACGATATCGTCGtt                                                                                                                                                                                                               <  2:199320/250‑1 (MQ=255)                                        tGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTg                                                                                                                                                                                                              >  2:402583/1‑251 (MQ=255)                                           gctgctGCGCTTTACCCATCCGTTTATGGGCTTCGCCGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCAGGTACCGGTGACGTTAACACCGCGCCTGGCCACGGCCCGGCCGACTCTGTGCTCGGTCAGAAATACGGCCTGGAAACCGCGAACCCGGCTGGCCCGGACGGCACTTATCTGCCGGGAACTTATCCGACGTTGGATGGCGTGACCGTCTTCAAAGCGAACGACCTAGTCGTGgcgc                                                                                                                                                                                                           >  2:423925/1‑251 (MQ=255)                                              gctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACGTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCgct                                                                                                                                                                                                          <  1:396908/249‑1 (MQ=255)                                               ctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCgctgct                                                                                                                                                                                                       >  1:219182/1‑251 (MQ=255)                                                 gcgcTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGctgctg                                                                                                                                                                                                      <  2:137148/250‑1 (MQ=255)                                                  cgcTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCa                                                                                                                                                                                                    <  1:254088/251‑1 (MQ=255)                                                   gcTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTTGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCtgctgccg                                                                                                                                                                                                   >  1:135462/1‑249 (MQ=255)                                                     tttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAgg                                                                                                                                                                                                  >  1:13860/1‑250 (MQ=255)                                                         cccATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGaaaa                                                                                                                                                                                              >  1:182525/1‑250 (MQ=255)                                                           cATCCGTTTATGGCCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAgg                                                                                                                                                                                           >  1:140221/1‑251 (MQ=255)                                                             tCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGACCGACATCGAAGTTGCGCTGCTGCAGGCAAAAcgcg                                                                                                                                                                                         >  2:159493/1‑251 (MQ=255)                                                              ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGTCCCTGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGgcg                                                                                                                                                                                         <  1:200024/250‑1 (MQ=255)                                                               cGTTTATGGAATTCGAAGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACCCCGCGCCTGGCCACGGCCCGGACGACTCTGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCAGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGATGCTGCAGGAAAAAGgagcg                                                                                                                                                                                       >  2:283318/1‑251 (MQ=255)                                                                          ttCGACGTTACGGCAATCCTCGGCGATCACGTTCCCCTGGATGACGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCCCTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGACCGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGATGCCCGt                                                                                                                                                                            >  2:422336/1‑251 (MQ=255)                                                                                    cGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGGACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGCCTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAATGCACGCAGCTGCACGTTTAGAAAATg                                                                                                                                                                  >  2:62627/1‑251 (MQ=255)                                                                                    cGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATg                                                                                                                                                                  >  1:233652/1‑251 (MQ=255)                                                                                     ggCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGGCGTTGCGCTGCTGCAGGAAAAAGGCGCGCAGCTGCACGTTGAGCAAATg                                                                                                                                                                  >  2:367748/1‑250 (MQ=255)                                                                                       caagccTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTATGCCGGTTGGCCCGGACGGCACTTATCTGGCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGTCATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATgcag                                                                                                                                                               <  2:117584/247‑1 (MQ=255)                                                                                           ccTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGcaca                                                                                                                                                           <  2:5161/251‑1 (MQ=255)                                                                                                  gaccACGTTACCTTGGATTCCGGTACCGGTGCCGTTCCCACCGCGCCTGGCCACGGCCCTGACTGCTATGTGAATGGCCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGTGTGAACGTCTTCAAAGCGAATGACATCGTCGTTGCGCTGCTTCAGGAAAAAGGCGCGCTGCTGCACGTTGAGATAATGCATCACAGCTATcc                                                                                                                                                    <  2:400057/248‑1 (MQ=255)                                                                                                   aTCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCg                                                                                                                                                   >  1:332682/1‑251 (MQ=255)                                                                                                     cACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGtg                                                                                                                                                 >  1:26736/1‑251 (MQ=255)                                                                                                        gTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGtgctg                                                                                                                                              <  1:396406/251‑1 (MQ=255)                                                                                                             ccTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGCTCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGc                                                                                                                                         >  2:143281/1‑251 (MQ=255)                                                                                                                ggATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTc                                                                                                                                      >  1:93530/1‑251 (MQ=255)                                                                                                                     ccGGTTCCGGTGCCGTTCACACCGCGCCCGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACGGCCGGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCGTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCGGCTGCACGTGGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACaaa                                                                                                                                 <  2:17769/251‑1 (MQ=255)                                                                                                                     ccGGTACCGGTGCCGTTCACACCGAGCCTGGCCACGGCCCGGACGACTATGAGATCGGTCCGAACTACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTCTCCGACGATGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGACGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGAACGTTGAGAAAATGACGCACAGCTTTCCGTGCTGCTGGCGACACaa                                                                                                                                  >  2:42351/1‑250 (MQ=255)                                                                                                                     ccGGTACCGGGGCCGTTCACCCCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTTGCGTCACaaa                                                                                                                                 <  2:304403/251‑1 (MQ=255)                                                                                                                        gTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACg                                                                                                                              >  1:128554/1‑251 (MQ=255)                                                                                                                             ggTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAAAGCAGCCCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGat                                                                                                                         >  2:363383/1‑251 (MQ=255)                                                                                                                                gCCGTTCCCACCGCGCCAGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGACTGGAAACCGCTAACCCGGTTGGCCCGGGCGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGTACGACATCGTCGTTGCGCTTCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGatcat                                                                                                                      <  1:418964/251‑1 (MQ=255)                                                                                                                                gCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGatcat                                                                                                                      >  2:163511/1‑251 (MQ=255)                                                                                                                                gCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGatca                                                                                                                       <  1:104470/250‑1 (MQ=255)                                                                                                                                               ccTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCTGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGGACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCGGTTGCACGTTGAGAAAATGCAGCACAGTTGTCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgcc                                                                                                       <  1:181214/251‑1 (MQ=255)                                                                                                                                                cTGGCCGCGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGGTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGGCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCTTGCTGCTGGCGTca                                                                                                                                     <  2:59307/220‑1 (MQ=255)                                                                                                                                                cTGGCCACGGCCCGGCCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATACGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTca                                                                                                                                     >  1:59307/1‑220 (MQ=255)                                                                                                                                                cTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCCCGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgccg                                                                                                      >  2:343993/1‑251 (MQ=255)                                                                                                                                                cTGGCCACGGCCCGGACGAATATGTGATCGGTCCGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTCTCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACCGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGGGCGccgccg                                                                                                      >  1:336878/1‑251 (MQ=255)                                                                                                                                                  ggcaacggcactgacgacGATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGACCGGACGGCAATTATCTGCCGGGCACTTATCCGACGTTGGAGGGCGTGAACGTATTCAAAGCGAACGACATCGTCGTGGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTAGCCGTGCTGCGGGCGTCACAAAACGCCGATCATCTTCCGCGCGAcgccgc                                                                                                     <  2:199881/238‑1 (MQ=255)                                                                                                                                                   gCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCGGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAg                                                                                                   <  2:345232/251‑1 (MQ=255)                                                                                                                                                     cACGGCCCGGAAGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTCTCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTg                                                                                                 >  2:331858/1‑251 (MQ=255)                                                                                                                                                         gCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACGTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTGCCGCGCGACGCCGCAGTGGTTc                                                                                             <  2:214598/251‑1 (MQ=255)                                                                                                                                                           ccGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGt                                                                                           >  1:48019/1‑251 (MQ=255)                                                                                                                                                            cGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTCGTc                                                                                          >  2:336452/1‑251 (MQ=255)                                                                                                                                                               acgacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGc                                                                                       >  1:105260/1‑251 (MQ=255)                                                                                                                                                                 gacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCGAGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAGCGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCCGCACAGCTATCAGTGCTGCTGGCGTCACAAAACGCCGTTCATCTTACGCGCGACGCCGCAGTGGTTCGTCAgaat                                                                                     >  2:225291/1‑248 (MQ=255)                                                                                                                                                                 gacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCTACGCCGCAGTGGTTCGTCAGCAt                                                                                     >  2:165356/1‑251 (MQ=255)                                                                                                                                                                   cTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGGTGGCCCGGACGGCACTTATCTGCCGGGAACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAAAGACATCGTCGTTGCGCTGCTGCAGGAAAAAGTAGAGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATACGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGAGACGCCGCAGTGGTTCGTCAgactgg                                                                                   >  1:403069/1‑246 (MQ=255)                                                                                                                                                                     aTGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCGGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCGGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCATCATgg                                                                                   <  2:220095/249‑1 (MQ=255)                                                                                                                                                                          aTCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCAAGGATCAGa                                                                             >  1:57069/1‑250 (MQ=255)                                                                                                                                                                          aTCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGACGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATAATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGa                                                                             >  1:399273/1‑250 (MQ=255)                                                                                                                                                                            cGGTCAGAACTACGGACTGGCCACCGCTAACCCGGTTGGCCCGGACGGCACTTATATGCCGGGCACTTATCCGCCGTTGGATGGCGTGACCGTCTTCAAAGCGAACGACCTCGTCGTTGCGCTGCTGCCGGAAAAAGGCGCGCTGCTGCACGTTGCGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGaa                                                                            >  2:465704/1‑249 (MQ=255)                                                                                                                                                                              gTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCTCTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCAGCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGt                                                                        <  1:283318/251‑1 (MQ=255)                                                                                                                                                                                    aaTACGGCCTGGAAACCGCTAACCCGGTTGGCCCCGACGGCTCTTATCTGCCGGGCACTTAGCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGTAGTGGTTCGTCAGCATGGAGCAGAAAGGTCtgcgt                                                                  <  2:336878/251‑1 (MQ=255)                                                                                                                                                                                      tACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgcgtgc                                                                >  1:351961/1‑251 (MQ=255)                                                                                                                                                                                          gCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTgcgc                                                              >  2:181503/1‑249 (MQ=255)                                                                                                                                                                                             gggAAACCGCTAGCCCGGTTGGCCCTGACGGCACTTATCTGCCGGGCGCTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACATCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTGCGTCAGCATGGATCAGAAAGGTCTGCGGGCGCAGTCa                                                         <  2:392867/250‑1 (MQ=255)                                                                                                                                                                                              ggAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTc                                                          <  1:197811/249‑1 (MQ=255)                                                                                                                                                                                              ggAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCa                                                         <  1:244765/250‑1 (MQ=255)                                                                                                                                                                                               gAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACt                                                       <  1:221280/251‑1 (MQ=255)                                                                                                                                                                                               gAAACCGCTAACCCGGTCGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACt                                                       >  1:97887/1‑251 (MQ=255)                                                                                                                                                                                                    cGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGACCGACATCGTCGTTGCGCTGCTGCGGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCCGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGaa                                                    >  2:437918/1‑249 (MQ=255)                                                                                                                                                                                                    cGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACATTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTAGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGATATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGaa                                                    >  1:64369/1‑249 (MQ=255)                                                                                                                                                                                                        aaCCCGGTTGGCCCGGCCGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGACCGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGACAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAag                                                  >  1:41989/1‑247 (MQ=255)                                                                                                                                                                                                        aaCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAGAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAgaga                                               <  2:93530/250‑1 (MQ=255)                                                                                                                                                                                                        aaCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGGGAACGTCTGCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAAGGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCAGCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAgaga                                               <  1:290163/250‑1 (MQ=255)                                                                                                                                                                                                          cccGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGCAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCCGTCACTGAAAGAGATc                                             >  2:341587/1‑250 (MQ=255)                                                                                                                                                                                                              gTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCCGCGTGCGCAGTCACTGAAAGAGATCAAAgg                                        >  1:123709/1‑251 (MQ=255)                                                                                                                                                                                                                 ggCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGAGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGACAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGt                                     >  1:38534/1‑251 (MQ=255)                                                                                                                                                                                                                   cccGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGc                                   <  1:400651/251‑1 (MQ=255)                                                                                                                                                                                                                    ccGGACGGAACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACACAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGTGTGc                                   >  2:370430/1‑250 (MQ=255)                                                                                                                                                                                                                         cGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGa                             <  1:110156/251‑1 (MQ=255)                                                                                                                                                                                                                         cGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATAGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTgg                              >  1:223972/1‑250 (MQ=255)                                                                                                                                                                                                                          ggCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGAt                            <  2:349869/251‑1 (MQ=255)                                                                                                                                                                                                                            cACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATcc                          >  2:166024/1‑251 (MQ=255)                                                                                                                                                                                                                               ttATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTCGTCAGCATGGATCAGTAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCgg                       >  2:86607/1‑251 (MQ=255)                                                                                                                                                                                                                                tATCTGCCGAGCACTTATCCGCCGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGTATCCCggc                      >  2:93699/1‑250 (MQ=255)                                                                                                                                                                                                                                tATCTACCGGGCACTTCTCCGACGTTGGACGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGATGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGCGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTAACAAAACGCCGATCATCTTCCGCGCGACGCAGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCGGTGGATCACgg                       >  2:441185/1‑250 (MQ=255)                                                                                                                                                                                                                                         ggcccTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGCTCATCTTCCGCGCGACGCCGCCGTGGTTCGTCAGCCTGGAACAGAAAGGGCTGCGTGCGCAGTCCCTGAAAGAGCTCAAAGGCGTGCAGTGGATCCAGGACTggggacg             >  2:245601/5‑248 (MQ=255)                                                                                                                                                                                                                                         ggCACTGATCCGACGTTGGATGGCGTGAACGGCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCAAGGATCAGAAAGGTCTGCGTGCGCAGGCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCa             <  2:57069/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGcc              >  1:37113/1‑249 (MQ=255)                                                                                                                                                                                                                                          gCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGcc              >  1:6350/1‑249 (MQ=255)                                                                                                                                                                                                                                            aCTTATCCCACGTTGGAAGGCGCGAACGTCTTCAGAGCGAACGACATCGTCGTTGCGCTGCTGCAGGACAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGCCGTCACAAACCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCACAGGCGTGCAGTGGATCCCGGACTGGGGCCAgac          >  2:179170/1‑249 (MQ=255)                                                                                                                                                                                                                                                    gACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCg  >  2:120927/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCG  >  NC_000913/23254‑23747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑