| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,332,310 | C→T | G321G (GGG→GGA) | basS ← | sensor histidine kinase BasS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,332,310 | 0 | C | T | 95.5% | 61.1 / ‑4.6 | 22 | G321G (GGG→GGA) | basS | sensor histidine kinase BasS |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (20/1); total (20/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
CTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGC > NC_000913/4332072‑4332551 | ctctGACGCGCATACTCTACTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGTCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATc > 1:348296/1‑251 (MQ=255) gACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCg < 2:181223/250‑1 (MQ=255) ctccAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGACAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTcc > 1:379067/1‑250 (MQ=255) cAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGCTTAACTACCGTGTTCAGCGTGCTGGTCGTCAGCAGATTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCAGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGCGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCt > 1:436011/1‑243 (MQ=255) tAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TAc > 1:183859/1‑250 (MQ=255) ggaggaGAGTGCAATGAAAACCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACg > 2:207262/1‑251 (MQ=255) agTGCAATGAAAAACCGAGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGTCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTCATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACt > 2:308847/1‑251 (MQ=255) gAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTccc > 1:278543/1‑251 (MQ=255) aaGTTTCACTACAGTGTTCAGCGTGCTGGTGGTCAGCAGCGTTCTTTATATCTGGTCTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAACACTGCCCGTGATGCAGCTGTGTAATGCGGCTGACAATACTTAATCCCACACCATTACAGCCATAACGGCGGTCCATACGATA‑AACCTCTTTACTCACCTCCACGCagttcatctactca > 2:145989/1‑237 (MQ=255) gTTTAACTCCCGAGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCAGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATGCAACTGTGTAATGCGGCTGACACTCCTTAATCCCCGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTATCCTCCACTCCCCACATTTACTCtcatcatt > 2:431765/1‑248 (MQ=255) tAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGACGTCTCTTGCAGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCt > 2:291624/1‑251 (MQ=255) cTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTAc > 1:285229/1‑234 (MQ=255) cGTGCTAGCGGTCCGCAGCGTTCTTTACATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTCCCCAGGCCCGCGTGCCGCAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCCATCCCGCCATACCGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATCCCTGGTCCTTCATCTTCa > 2:406055/1‑250 (MQ=255) cTGGTTTGCCACGTACTGCTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGc > 1:361712/1‑250 (MQ=255) gTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATa > 2:30027/1‑250 (MQ=255) ccGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCACCTGTGTAATGCGGCTGACAATACTTAATCCCAGCCCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCCTTTACTCTCCTCAATCCCTGGTCCTTCATCTTCAACTGCCATGCCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGc > 2:194213/1‑251 (MQ=255) aGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGACCAACTGCCCGTGATGCGACTGTGTAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACACACGCTTTACTCAACTCCCCGCAt > 2:36012/1‑149 (MQ=255) ccGGAAGTCTCGTCCCGGATTTGAAGGCAAAACTGCCCGTGAAGCCACTGTGTAATGCGGCTGACCATACTTACTCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTCCTCAACTCCCCCCATTTACTCTCATCAATAACAGGTCCTTCATCTTCACCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTCATCCTAATGTTGCTCCCTTGCGGGCTGTAACGATGGGCGtttt > 2:413206/1‑250 (MQ=255) ctgTGATGCAACTGTGTAATCCGGCTGACACTACTTAATCCCAGACCAATCCAGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCACAACCCCGCATTTACTCTCATCAATACCTGGTCCTGCATCTTCAACTGCAATGACCCCCCAGTCATCTTCTTGCAGCTTACTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATGCGAAGCAgggatac > 2:118769/3‑247 (MQ=255) tAATGCGGCTGACAATACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg > 2:352074/1‑251 (MQ=255) ggtgtccattctctacccccACACCAACCCCGCCATAACGGCTGTCCATACG‑TACAAACGTTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGGCATGACCGCTCCGTCATCTTCTTGCAGCTTAAGCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg < 1:78231/235‑1 (MQ=255) ggacataacgcaaCCCCACACCATCCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAAGAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggcg < 1:194213/240‑1 (MQ=255) aaTACTTAATCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGgcgc > 1:132436/1‑251 (MQ=255) | CTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACG‑TACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGC > NC_000913/4332072‑4332551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |