Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,311,337	C→T	A236T (GCA→ACA)	alsB ←	D‑allose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,311,337	0	C	T	100.0%	200.7 / NA	59	A236T (GCA→ACA)	alsB	D‑allose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/29);  total (30/29)

TCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCAGC  >  NC_000913/4311090‑4311583                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       tcCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACAAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGAc                                                                                                                                                                                                                                                     >  1:381332/1‑251 (MQ=255)    ggCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGc                                                                                                                                                                                                                                                  >  2:178695/1‑251 (MQ=255)             cAGTTTTATTGAGTGACCAGGATTGAACCGACCAGTTTAAATTCCTGTGCTTTATCCAGCGGGATTACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTTCTCCGATATCCGCCGGGTTCTTGGCAACCTTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCTACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAac                                                                                                                                                                                                                                         <  1:437111/251‑1 (MQ=255)              aGTTTGATTGAGTGACCAGGATTGAATCGACCATTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGTCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTTCCTGAGCAaca                                                                                                                                                                                                                                        <  2:353190/251‑1 (MQ=255)                ttttATTGAGTGCCCAGGAGTGAATCGACCAGTTTAAATACCGGTGCTTTATACCCAGGGATAACCTCCCCGGCTTTCTCAACGTCAACCCTCAGCTTAAGACCCATTGCGCCGATATCCGCCGGGTTCTGGGCAACAGTCACAATCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTCTCGGGCGTTTGTGACTGCCTGAGCCACAcc                                                                                                                                                                                                                                      >  2:165801/1‑251 (MQ=255)                    aTTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCAtt                                                                                                                                                                                                                                  >  2:342086/1‑251 (MQ=255)                           gACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGCCGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCg                                                                                                                                                                                                                           >  2:169273/1‑251 (MQ=255)                                 gATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCAt                                                                                                                                                                                                                     >  2:170678/1‑251 (MQ=255)                                    tGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTcg                                                                                                                                                                                                                  >  1:365938/1‑251 (MQ=255)                                    tGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTc                                                                                                                                                                                                                   <  2:124828/250‑1 (MQ=255)                                     gAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTcg                                                                                                                                                                                                                  <  1:427623/250‑1 (MQ=255)                                      aaTCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTcgcg                                                                                                                                                                                                                >  2:2387/1‑251 (MQ=255)                                                    aaTTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCttt                                                                                                                                                                                                  <  1:292393/251‑1 (MQ=255)                                                           gTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTc                                                                                                                                                                                           >  1:223653/1‑251 (MQ=255)                                                              cTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCgg                                                                                                                                                                                         >  1:169463/1‑250 (MQ=255)                                                                ttATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGa                                                                                                                                                                                        <  1:420169/249‑1 (MQ=255)                                                                ttATCCAGCGGGATAACCTTGCCGGATTTCTCAGAGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTACTATTCGGAtt                                                                                                                                                                                      >  1:344228/1‑251 (MQ=255)                                                                       gCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGc                                                                                                                                                                               <  1:310658/251‑1 (MQ=255)                                                                        cGGGATACCCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGc                                                                                                                                                                               >  2:300920/1‑250 (MQ=255)                                                                         gggATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCaa                                                                                                                                                                             <  1:160237/251‑1 (MQ=255)                                                                         gggATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGACACACCCATTGCAATCGTGTCATTCGCGCACTAGATCGCTTTAATATTCGGATTACGTTGcg                                                                                                                                                                              >  2:9770/1‑249 (MQ=255)                                                                               aCCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTcgc                                                                                                                                                                                                                 >  2:155464/1‑209 (MQ=255)                                                                               aCCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTcgc                                                                                                                                                                                                                 <  1:155464/209‑1 (MQ=255)                                                                               aCCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGtt                                                                                                                                                                       <  1:2387/251‑1 (MQ=255)                                                                                ccTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGtt                                                                                                                                                                       >  1:21263/1‑250 (MQ=255)                                                                                   tGCCGGATTTCTCAGCGTCAACCATCAGCTTCCGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCaa                                                                                                                                                                                                             >  2:229215/1‑209 (MQ=255)                                                                                   tGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCaa                                                                                                                                                                                                             <  1:229215/209‑1 (MQ=255)                                                                                    gCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGAATTACGTTGCAACACGTTAGTgg                                                                                                                                                                  >  2:384306/1‑251 (MQ=255)                                                                                        gATTTCGCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCAGTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACGTGTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGAGTACGTTGCAACACGTTAGTGGCGAc                                                                                                                                                              <  2:368639/251‑1 (MQ=255)                                                                                                    tCAACCATCTGCCTCAGACCAGTTGCGCCGATATCCGACGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCAAACCTTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCAGTTTTTCCGGCGTTTGTGACTGCCTGAGAAACACCCATTGCCATCGTGTCATTCGCGCAATAGCTCGCCTTACTATTCGGATTGCGTTGCAACACGTTAGTGGCGACATCCAGTGCtat                                                                                                                                                  >  2:279503/1‑249 (MQ=255)                                                                                                         cATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGc                                                                                                                                             <  1:169273/251‑1 (MQ=255)                                                                                                                   aGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTCCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTc                                                                                                                                   <  2:44311/251‑1 (MQ=255)                                                                                                                        cGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGc                                                                                                                                >  2:213986/1‑249 (MQ=255)                                                                                                                                cGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGgctggctg                                                                                                                      >  2:84524/1‑251 (MQ=255)                                                                                                                                      ccgccgGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACa                                                                                                                <  1:84524/251‑1 (MQ=255)                                                                                                                                                   ggCATCCGTCGCGATCATTTGGCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACCCCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGgc                                                                                                   <  2:7620/251‑1 (MQ=255)                                                                                                                                                     cAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGgctt                                                                                                 >  1:27904/1‑251 (MQ=255)                                                                                                                                                       tccGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGgc                                                                                                   <  2:287559/246‑1 (MQ=255)                                                                                                                                                       aCCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGgc                                                                                                   >  1:287559/1‑247 (MQ=255)                                                                                                                                                          gTCGCGGTCATTTGTCCGGCTTCTACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACTTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCttt                                                                                            <  1:342086/251‑1 (MQ=255)                                                                                                                                                                     ttGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCATGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCt                                                                                  <  1:288980/250‑1 (MQ=255)                                                                                                                                                                         ccGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGt                                                                             >  1:93101/1‑251 (MQ=255)                                                                                                                                                                         ccGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGAAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCGTTTTTGAAGGCTTCGGt                                                                             <  2:223653/251‑1 (MQ=255)                                                                                                                                                                           ggCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTgg                                                                           <  1:279503/251‑1 (MQ=255)                                                                                                                                                                               tCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCAc                                                                        <  1:213986/250‑1 (MQ=255)                                                                                                                                                                               tCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCAc                                                                        <  2:364034/250‑1 (MQ=255)                                                                                                                                                                                                         gccctCTGTCCCGACGACCAGCCCTTTTCCCGTTCTTCCTGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCAGTCGCGCAATAGATCGCTTTAAGATTCGTATTACGTTGCAACTCGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTAATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGt                                             <  2:260394/247‑1 (MQ=255)                                                                                                                                                                                                                gtagctacgacCAGCCCTGTTCCCGTGTTTCCGGCGTTTGTGTATGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCTCTTTAATATGCGGAGTCCGTTGCAACACGTTAGTGGCGACATCCAGGTCTTTAATGCGTTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTTATCTGGCTTGATTTTGTGAAGGCTTCGGTGGCACCATTCCGACGCGTGTCACCGGAGGCGTTAcc                                         <  2:314320/242‑1 (MQ=255)                                                                                                                                                                                                                gTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCgg                                       <  1:156485/250‑1 (MQ=255)                                                                                                                                                                                                                    cgacgaCCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCttt                                   >  1:216450/1‑250 (MQ=255)                                                                                                                                                                                                                              cACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGAc                                                                                                                                                              <  2:26080/117‑1 (MQ=255)                                                                                                                                                                                                                              cACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGAc                                                                                                                                                              >  1:26080/1‑117 (MQ=255)                                                                                                                                                                                                                               aCTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGAt                       >  2:24200/1‑251 (MQ=255)                                                                                                                                                                                                                                  tttCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGAtt                      <  1:178695/249‑1 (MQ=255)                                                                                                                                                                                                                                             tCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTcaccac         >  1:107980/1‑251 (MQ=255)                                                                                                                                                                                                                                                ggCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCtt      >  2:237689/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATATATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCAg   <  1:362224/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTc     >  1:438535/1‑249 (MQ=255)                                                                                                                                                                                                                                                    tttGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCAgc  >  2:134140/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       TCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCAGC  >  NC_000913/4311090‑4311583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑