Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,303,755	C→T	V119I (GTT→ATT)	mdtN ←	putative multidrug efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,303,755	0	C	T	97.7%	131.9 / NA	43	V119I (GTT→ATT)	mdtN	putative multidrug efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/23);  minor base G (0/1);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTT  >  NC_000913/4303516‑4303996                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   atCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCAc                                                                                                                                                                                                                                         >  1:397858/1‑251 (MQ=255)          gACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCACCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAAACTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCAACCGTGGCATTAATCGAGTCGGCACCAAAATGtt                                                                                                                                                                                                                                >  1:200227/1‑251 (MQ=255)                            aaCTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGATTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTCATGTATCTGTGGCCTGTTTCGCGGCGGCAAGGGCTTTTTTTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑acgctccgct                                                                                                                                                                                                             >  2:60884/1‑251 (MQ=255)                                  tGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTg                                                                                                                                                                                                        >  2:363046/1‑250 (MQ=255)                                      tCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGGCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCAt                                                                                                                                                                                                   >  1:216503/1‑251 (MQ=255)                                         aCGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGGATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGGGAGCATAAt                                                                                                                                                                                                >  2:113531/1‑251 (MQ=255)                                         aCGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTTAGCATAAt                                                                                                                                                                                                <  1:327805/251‑1 (MQ=255)                                         aCGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAAt                                                                                                                                                                                                <  1:162830/251‑1 (MQ=255)                                                 gACGGCTCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTAt                                                                                                                                                                                        <  1:363262/251‑1 (MQ=255)                                                 gACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTa                                                                                                                                                                                         <  1:375319/250‑1 (MQ=255)                                                  aCGGCGCTGGCGGCTGACTGCGCCTGGAACAATACGGCAGTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATc                                                                                                                                                                                       <  1:448687/251‑1 (MQ=255)                                                   cGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCCCGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTCCGCTGGGTGAGCATAATTTGCTTTTcc                                                                                                                                                                                      >  2:51205/1‑251 (MQ=255)                                                    ggCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCCTTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACTCTGGGTGGGCATAATTTGCTTATcc                                                                                                                                                                                      >  2:392624/1‑250 (MQ=255)                                                            cggcTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCcgcg                                                                                                                                                                             <  2:356685/251‑1 (MQ=255)                                                                 gACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgagg                                                                                                                                                                          <  1:184453/249‑1 (MQ=255)                                                                     gcgcCTGTAACAATACGGCATTAAGATCCGCTTCTGCGTCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgagggagg                                                                                                                                                                      <  2:322293/249‑1 (MQ=255)                                                                     gcgcCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGTCGCgagggagg                                                                                                                                                                      <  2:306347/249‑1 (MQ=255)                                                                      cgcCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGGAATGGCTCGGTGCGGCGTAATGTATCTGGGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTTCCGTGGCATTAATCGAGACGTCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGCGCATAATTTGCTTATCCCGCGCCGCGAGGGAGGCtt                                                                                                                                                                   >  2:247030/1‑251 (MQ=255)                                                                           gTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCt                                                                                                                                                              <  1:157868/251‑1 (MQ=255)                                                                                    cGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAAGTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGa                                                                                                                                                     <  2:111325/251‑1 (MQ=255)                                                                                              aTCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGt                                                                                                                                           >  1:305574/1‑251 (MQ=255)                                                                                                  gCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACgg                                                                                                                                       <  1:60884/251‑1 (MQ=255)                                                                                                     tCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTGCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTGCGTACGGgc                                                                                                                                     <  2:261657/250‑1 (MQ=255)                                                                                                              cgcTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGAt                                                                                                                           >  2:79493/1‑251 (MQ=255)                                                                                                               gcTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATg                                                                                                                          <  1:265090/251‑1 (MQ=255)                                                                                                                       gTTCTTCCACGGTCAACTTCTTCCGCTGAGGCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCGGTTGCCCGTCTCGCGGCGGCACGGTCTTTTGCGCCCGTGGCAGTAAGCGAGGCGGCACCTAACCGTTTCGCGTCA‑ACGCTACGCTGGGTGTACATAATTTGCTTATCCAGTGACGCGAGGGAGGCTGCATGTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAg                                                                                                                  <  1:263704/251‑1 (MQ=255)                                                                                                                                            tCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACt                                                                                               <  1:363046/249‑1 (MQ=255)                                                                                                                                                              tcttTCAGTAATGGCTCGGTGAGGCGTAATGTATATGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGCGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGGTTGACTGCCTGGTTGTCGGGGACCGc                                                                           >  2:440294/1‑251 (MQ=255)                                                                                                                                                               cttTCAGTCATGGCTCGGTGCGGCGTAATGTATATGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGcc                                                                          >  1:375417/1‑251 (MQ=255)                                                                                                                                                                  tCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGt                                                                       >  1:445448/1‑251 (MQ=255)                                                                                                                                                                    aGTAATCGCTCGGTGCGGCGCAATGGATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATACTTTGATTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGGTTGGATTCGTACGGGCGCGGGGCGATGCGGAACAACAAATCGCCCTGTTTGACTGCCTGGTTTTTGGTTATCGCCAGtt                                                                      >  2:114265/1‑250 (MQ=255)                                                                                                                                                                      aaaTGGTTCGGTGCGGTGTAATGTATCTGTGGCTTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGTTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGTTTCAGCTTTCGCCAGATTGGCTTCGTACGGGGGCGGGTCGATGCGGAACAGCAAAGCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTa                                                                   <  1:281275/250‑1 (MQ=255)                                                                                                                                                                                   cggcgTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCt                                                      >  2:83006/1‑251 (MQ=255)                                                                                                                                                                                                  gtgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTAcc                                         <  1:99070/249‑1 (MQ=255)                                                                                                                                                                                                                      aCGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAACTCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGAcg                   >  1:236560/1‑251 (MQ=255)                                                                                                                                                                                                                            tttttCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTc                                                                                                                              >  1:392717/1‑138 (MQ=255)                                                                                                                                                                                                                            tttttCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTGTCGCCAGATTGGCTTCGTACGGGCGCGGGTc                                                                                                                              <  2:392717/138‑1 (MQ=255)                                                                                                                                                                                                                             ttttCTCCCGTGGCGTTAATCGAGTCGGCACCAAACTGGTGCGCGTCA‑CCGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAg             <  2:156078/250‑1 (MQ=255)                                                                                                                                                                                                                                   ccccggggcttAATCGAGTCGGCACCAATGTGTGGAGCG‑CAGACGCTACTCGGGGTGAGCATAATAGGCTTAGCCAGGCCCGCGAGGTGGGGTTTAGCTTTCGCCAGATTGGCTACGTAGGGGTGCGGGTCGATGCGGAATATCAAGTCGCCCGGGTGGACTGCCGGGTTGGGGGTGCCCGCTAGTTCTACAATGCGGGCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCAtt      <  1:448637/242‑1 (MQ=255)                                                                                                                                                                                                                                   aCCGTGTCATTAATCGAGTCGGCCCCAAACTGTGGCGCGTCA‑ACGCTACGCTGTGTGAGCATAATTTGCTTAGCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCTTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCAt       <  1:247030/250‑1 (MQ=255)                                                                                                                                                                                                                                   aCCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGCCTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCa        <  1:121306/249‑1 (MQ=255)                                                                                                                                                                                                                                    ccGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTa     <  1:79493/251‑1 (MQ=255)                                                                                                                                                                                                                                       tGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGtt  >  1:457529/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ATCCGCTTCGACCGCCGCACGCTGGGCAACTAATGCATCCACGCCGCTGACGGCGCTGGCGGCTGACTGCGCCTGTAACAATACGGCATTAAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCA‑ACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGGTACCACATCAATGGTATCTGCTGACGCGTAAGCGTCATTAGTT  >  NC_000913/4303516‑4303996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑