Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,301,071	C→T	A670T (GCA→ACA)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,301,071	0	C	T	98.0%	138.9 / ‑3.9	50	A670T (GCA→ACA)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (24/25);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

AGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCCAGGCGTG  >  NC_000913/4300825‑4301314                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGCTGAATATCGTCGGCCAGTTTGATTTGCTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCggg                                                                                                                                                                                                                                                   >  1:66397/1‑251 (MQ=255) aGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGACTGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCggg                                                                                                                                                                                                                                                   >  1:135136/1‑251 (MQ=255)   cTGCGGGTCATTGCGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCCCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCggggg                                                                                                                                                                                                                                                 >  2:159880/1‑250 (MQ=255)        ggTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTcc                                                                                                                                                                                                                                            >  1:378787/1‑251 (MQ=255)                        ccaccaCTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGc                                                                                                                                                                                                                           <  1:38349/251‑1 (MQ=255)                        ccaccaCTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTAAGGTTTGCGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCCCCACAGCAGCCGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGGTGTcccggttggccagcgggg                                                                                                                                                                                                                            >  2:434687/1‑235 (MQ=255)                                gctccgtcGGCCTGCCGGAGCTGGCAAGATGAATATCGTCGGCCATTTTGATTTTTGTCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGTCACAGCCTGAAATCAGCGGCGTTCTGCCTAGAATTCTGCACAACAGCAGATGTGAAAGTTGACGATTTTTCAGTGGGTTGCTCCTTTTTCCTTTTCCGTGTCGGTCGTGAGTGTCGGGTCTGTTCATGT‑TGGCAGGCGTGCGAAAAg                                                                                                                                                                                                                     <  2:52544/242‑1 (MQ=255)                                gcgcCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACACCAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCt                                                                                                                                                                                                                   >  1:214433/1‑251 (MQ=255)                                gcgcCTGCGGCCAGCAGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGACGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGaaaaacg                                                                                                                                                                                                                   >  1:352616/1‑248 (MQ=255)                                        ggCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACg                                                                                                                                                                                                           <  2:258261/251‑1 (MQ=255)                                                  aGCTGGCAAGATTAATATCGTCGGCCAGTTTGAGTTCTTCCTGTTTGAGCTTTTGATTAGTTGCAGAACCCTGACGTACCAGGGCCCATCCGGAAATCAGCGACGTGCTGCCTAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTTATCATTGCGTGTCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGTGGCTGTCCA‑GGCTGGCATTCGTTCGATAAGCTGGCTGACCTGTTGCTCCt                                                                                                                                                                                                 <  2:285236/251‑1 (MQ=255)                                                      ggCAAGATGAAGATCGTCGGCCAGTTTGATTTGTGCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACTATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGtt                                                                                                                                                                                              <  2:135136/250‑1 (MQ=255)                                                        cAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGttttt                                                                                                                                                                                           >  1:127490/1‑250 (MQ=255)                                                        cAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGAAGTGTTGCTCCTGttttt                                                                                                                                                                                           >  1:257176/1‑250 (MQ=255)                                                           gatgactctcgtcgGCCCGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GGCTGGCAAGCGGGCGAAAAGCTGGCCGCCGGGTTGCTCCTGTTTtaat                                                                                                                                                                                         >  2:301249/9‑250 (MQ=255)                                                                 tatCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGGCGTGTTGCTCCTGTTTTTATAAGGTgg                                                                                                                                                                                  >  2:402054/1‑251 (MQ=255)                                                                    cgtcgGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGc                                                                                                                                                                                >  1:28258/1‑250 (MQ=255)                                                                    cgtcgGCCAGTTTGATTTGGTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTAGCAGGGCACAGCCGGAAATCAGTGTCGTGCTGCCGAGAATGCTGCACAACAGTAGACGTGAAAGTTGACGATTGATCATTGCGTTGCTGCTTGTGCGTGTTCCGTGGCGGGTGTTAATGTCGGGGCTGTCCA‑GTCTGGGAAGCGGGGGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCa                                                                                                                                                                               <  1:284950/251‑1 (MQ=255)                                                                        ggCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGAt                                                                                                                                                                           <  1:59563/251‑1 (MQ=255)                                                                                       ttCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGGAAAGCTGGCAGACGGGTTGCTCCTGTTTTTATAAGGTGGGCAGGATGGCCTGAGAGGCggg                                                                                                                                                            >  2:129689/1‑251 (MQ=255)                                                                                          cGGTTTGAGCTGTTGAGGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtgt                                                                                                                                                         <  2:127490/251‑1 (MQ=255)                                                                                                     gTTGATGAGGTGCAGAATCCTTACGTACCAGGGCCAAGCCGGCAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACCGCAGACGTGAAAGTTGACGATTGATAATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATTTCGGGGTTGGCCA‑GTCTAGCAAGCGGGCGAAAAGCTGGCAGACGCGTTGCTACTGTTTTTATTAGGGGGGCAGGATGGACTGTGCGGCGGgtggagtctctaacc                                                                                                                                              >  2:427854/1‑239 (MQ=255)                                                                                                     gTTGATGAGGTGCAGAATCCTTACGGACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGATAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTTCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCg                                                                                                                                              <  2:313751/251‑1 (MQ=255)                                                                                                         atgaGGTGCAGACTCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACACCAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTCATGTCGGGGCTGTCCA‑GTCTGGAAAGCgg                                                                                                                                                                                                                             >  1:389426/1‑168 (MQ=255)                                                                                                         atgaGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTGCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCgg                                                                                                                                                                                                                             <  2:389426/168‑1 (MQ=255)                                                                                                           gaGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGGTGGCCTGCGAGGCGGgtgtattttgcagccgttctt                                                                                                                                        >  1:185111/1‑234 (MQ=255)                                                                                                             ggTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCgggggtttttttcaagtggtttttt                                                                                                                                      >  1:8715/1‑229 (MQ=255)                                                                                                              gTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCCGCAGAGAATGCTGCACACCAGCAGACGTGAAAGTTGACGTTTGATAATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGCGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAACGGCCGAACACCTGGCAGACGTGTTGCTCCTGTTTTCATAAGGGGGGGCGGATGGCCGGAGAGGCggggggatcttctaaggtttatttgg                                                                                                                                     >  1:169293/1‑228 (MQ=255)                                                                                                                 cAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTta                                                                                                                                                                                           >  1:2031/1‑194 (MQ=255)                                                                                                                 cAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTta                                                                                                                                                                                           <  2:2031/194‑1 (MQ=255)                                                                                                                     atacTTACGTACCAGGGCACAGCCTGAAAGCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTTACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCATGCTGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGTCGGTTGTCTCTTCAAGCGTTATTTTAGTAGAACg                                                                                                                              <  2:410927/248‑1 (MQ=255)                                                                                                                             gTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTGGACGATTGATCAGTGCGTGGCTCCTTGTGCCGGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTCAGGAGAACGGCTGAGTg                                                                                                                      <  2:266579/251‑1 (MQ=255)                                                                                                                                           ccGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGc                                                                                                                                                               >  2:121519/1‑196 (MQ=255)                                                                                                                                           ccGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGc                                                                                                                                                               <  1:121519/196‑1 (MQ=255)                                                                                                                                                 aTCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCGGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTgag                                                                                                                                     <  1:209248/216‑1 (MQ=255)                                                                                                                                                                            cacaACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGttt                                                                                                                                                                                             >  2:127142/1‑133 (MQ=255)                                                                                                                                                                            cacaACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGttt                                                                                                                                                                                             <  1:127142/133‑1 (MQ=255)                                                                                                                                                                               aacaGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTTCAGCGGTTTTTTAGGGGAAAGGGTGAGTGCGGTGGCCAGACCGGCCGGGTGTTTTTCCCGGGCGTCGG‑GAAACTGAccc                                                                   >  1:311801/1‑250 (MQ=255)                                                                                                                                                                                       aCGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTc                                                           <  2:71403/251‑1 (MQ=255)                                                                                                                                                                                           gAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCgg                                                        <  1:420457/250‑1 (MQ=255)                                                                                                                                                                                              aGTTGACGATTGATCATTGCGTGGCTCCTTGTGCATGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGt                                                    <  1:54250/251‑1 (MQ=255)                                                                                                                                                                                                 tGACGATTGATCATTGCGTGGCGCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCTGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTGAATAAGGTGGGCAGGATGGCCTGAGAGTCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAg                                                  <  1:270091/250‑1 (MQ=255)                                                                                                                                                                                                        tGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTGTCTTCCAGAGGTATTTGGGGGGAAAGGGGGGGGGCGGGTGGCAGAACCGCAGCGGTTTTTTCCCGGGCGGCGGGCAAAC‑GACCTGCTCTGTCCGGGTGTAggacggttt                                          >  1:221685/1‑243 (MQ=255)                                                                                                                                                                                                          aTCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGGAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAACGTTTTTTTTGGAGAACGGCTGGGTGCGGGTGCCAAAACGGCCGCGGATTTTTCCCGGGGGTCGG‑CCAAATAACCCGCTCTGTCCCGCTGGAGTGCggtttcc                                        >  1:61089/1‑245 (MQ=255)                                                                                                                                                                                                                 cGTGGCTCCTTGTGCCTTTGCCGTGGCGGGCGTTAATGCCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGTAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCt                                 <  1:57753/251‑1 (MQ=255)                                                                                                                                                                                                                           tgtgCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGt                        <  1:210247/250‑1 (MQ=255)                                                                                                                                                                                                                            gtgCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGtt                       <  2:378787/250‑1 (MQ=255)                                                                                                                                                                                                                                      cGTGGCGGGCGGTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCAt            <  1:274874/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggtgggcgGTAATGTAGGGGCTTTCCA‑GTCTGGCATGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTATTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATcc          <  1:166321/247‑1 (MQ=255)                                                                                                                                                                                                                                                gTTAATGTCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCCAGGCGTg  <  1:389969/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      AGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCA‑GTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGG‑CAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCCAGGCGTG  >  NC_000913/4300825‑4301314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑