Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,269	C→T	D4N (GAT→AAT)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,269	0	C	T	100.0%	91.1 / NA	28	D4N (GAT→AAT)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

CCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGTTGGG  >  NC_000913/662038‑662511                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   ccGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCAtct                                                                                                                                                                                                                                 >  1:103237/1‑251 (MQ=255)                      gCCCACCGCGATCGCTCTGGATAACCGGAATATCACCCGGCATTAAAATGCGCTCCGATTTTCCTGCCTGACCTTGGGTGAATCCCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAACACGGCATCTCCGTGGAGAATGAGTAAAAgg                                                                                                                                                                                                            >  2:311198/1‑250 (MQ=255)                          aCCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTgggg                                                                                                                                                                                                        <  1:385748/251‑1 (MQ=255)                                                     atCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGga                                                                                                                                                                                                                          <  1:252238/206‑1 (MQ=255)                                                     atCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGga                                                                                                                                                                                                                          >  2:252238/1‑206 (MQ=255)                                                               aTTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATAACAGCGAGGAGAGGGGAGTTACCCg                                                                                                                                                                   >  1:223998/1‑251 (MQ=255)                                                               aTTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATACGTAAAAGTGGGGGAAAAGTATATCACAGCGGGGAGGGGGGATGTACCCg                                                                                                                                                                   >  2:430107/1‑251 (MQ=255)                                                                   aaaTGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTTTTCTGATACAAAAAGGCATCTCCGTGGAGGATGAGTAAAAGTGGGGGAAAAGAATATCACAGCGAGGAGAGGGGGGTTACCCGAca                                                                                                                                                                >  2:314865/1‑249 (MQ=255)                                                                                 tttCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAAc                                                                                                                                                  <  2:338886/250‑1 (MQ=255)                                                                                            aCCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCgggt                                                                                                                                      >  2:30115/1‑248 (MQ=255)                                                                                            aCCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGt                                                                                                                                      <  1:350357/251‑1 (MQ=255)                                                                                                    ggAATACCGGATAGTGCTCGACCAGCCAGATTTCAGCAAGGGTACGATCATCGCGGGTATCGGTGAATTCATGCAGAGCCTGGGAGATTGGCGCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCGGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAAc                                                                                                                              <  2:142689/250‑1 (MQ=255)                                                                                                        aaCCGGATAGTGCTCGACCAGCCAGAGTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTGACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATg                                                                                                                          <  2:442464/250‑1 (MQ=255)                                                                                                                   gCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACCAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATc                                                                                                               >  1:271381/1‑251 (MQ=255)                                                                                                                         ccagccagATTTCATCAAGGGGACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGTAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAGCGGAGAAGGGAGGTACAGAACCTTTCGGACCATATCGAtttt                                                                                                         <  2:65640/251‑1 (MQ=255)                                                                                                                                                              aTCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGGAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGat                                                                     >  1:357849/1‑250 (MQ=255)                                                                                                                                                                     aaTTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGGGTTTCAACCTGCTCGATATGAGTgg                                                             <  2:242135/251‑1 (MQ=255)                                                                                                                                                                             cATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATAGCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTtgata                                                     <  1:24076/251‑1 (MQ=255)                                                                                                                                                                                           ttGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCg                                        >  1:440872/1‑250 (MQ=255)                                                                                                                                                                                                 cGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTgg                                   <  1:167076/249‑1 (MQ=255)                                                                                                                                                                                                  gTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAg                                <  1:250902/251‑1 (MQ=255)                                                                                                                                                                                                   tAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGTTAGTGATAGATACCGAGTGGTAGt                               >  1:245023/1‑251 (MQ=255)                                                                                                                                                                                                    aaGGCTGAAGACCGAGCTGGCGGACAAGAATTGTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCAGTCGGACAATATCGATTTTGGCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTtgatagtgatttagaccgagggttagtt                              <  2:213017/251‑19 (MQ=255)                                                                                                                                                                                                     aGGCTGAAGAACGATCTGGCGGACAAGAATTTTATTCTGATACAAAACGGAATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATAGCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTg                             <  2:245023/251‑1 (MQ=255)                                                                                                                                                                                                             gACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTtgct                     <  1:138624/251‑1 (MQ=255)                                                                                                                                                                                                                 gAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCtt                 <  1:30115/251‑1 (MQ=255)                                                                                                                                                                                                                             aaGAATTTTATTCTGATACAAAACGGCATCTCCGGGGCGAAGGAGTAAAAGTGGGGGAAAATTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTTCAGAACCATGCGGACAATATCGATTTTGCCCAGTTCGGCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGTTACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGtt     <  2:382182/251‑1 (MQ=255)                                                                                                                                                                                                                                aaTTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATCGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGTTggg  >  2:142090/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGTTGGG  >  NC_000913/662038‑662511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑