Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,298,199	C→T	A390T (GCA→ACA)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,298,199	0	C	T	98.8%	263.0 / NA	84	A390T (GCA→ACA)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (41/41);  minor base G (1/0);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGTTCAACCGCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGT  >  NC_000913/4297953‑4298445                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       tCGGTTCAACCGCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGtgcg                                                                                                                                                                                                                                                     <  1:172545/250‑1 (MQ=255)         cccGCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGAGCTTCGCCCAGAATGTACGCGGCACGCACTTCGCCATGCGCTGTTCGGTGCGGCAg                                                                                                                                                                                                                                            <  2:306827/250‑1 (MQ=255)          ccGCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTGTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCTTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGc                                                                                                                                                                                                                                           <  1:45790/251‑1 (MQ=255)           cGCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGcc                                                                                                                                                                                                                                          >  2:125302/1‑250 (MQ=255)            gCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTc                                                                                                                                                                                                                                         <  1:345412/251‑1 (MQ=255)             ccTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCg                                                                                                                                                                                                                                        <  2:95060/251‑1 (MQ=255)                tGAACAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCTGTTTTGGGCATAAAGATGTCCTGAACGATAACCAGTTTCAGATCTTCAAAGGCTTTACGTACTGCCGACATGTCCGCGTCAGTTTGTAGCGGATCTTCGCCCAGAATGTACGCTGCACGCACTTCGCCATGCGCTTTGCGGTGCGGCAGCTCGc                                                                                                                                                                                                                                       <  1:173271/249‑1 (MQ=255)                  aagaaACGCTGGAAGCCACGGGCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACTGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCTGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCTCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCGCTTCTCCATGCGCTGTGCGGTGCGGCGGCTCGCTGAt                                                                                                                                                                                                                                   <  2:223308/251‑1 (MQ=255)                        cGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATa                                                                                                                                                                                                                             <  2:391724/251‑1 (MQ=255)                        cGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGAGGCGAt                                                                                                                                                                                                                              >  2:11396/1‑250 (MQ=255)                         gCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGAt                                                                                                                                                                                                                              >  1:75195/1‑249 (MQ=255)                            ggAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGc                                                                                                                                                                                                                           <  2:266778/249‑1 (MQ=255)                            ggAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCg                                                                                                                                                                                                                         <  2:5990/251‑1 (MQ=255)                             gAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCCAACGCTTTACGGACTGCCGCCAGATCCGCGTCAGTTTGTCGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCGCACTGATGCGATAGcagg                                                                                                                                                                                                                        >  2:342168/1‑248 (MQ=255)                               aGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTa                                                                                                                                                                                                                      <  2:329703/251‑1 (MQ=255)                                 ccACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATg                                                                                                                                                                                                                    >  1:148095/1‑251 (MQ=255)                                              cAGTAAACACGCCTTCATGCTCGC‑CCCACGACGGTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGTGGCAGCTCGCTTATGCGATAGCCGGTATGCGCTGGCAGGCtt                                                                                                                                                                                                       <  1:146411/251‑1 (MQ=255)                                               aGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACACCCGCCGCCGACGCGGGTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCGTTACGTACTGCCGACAGCTCCGCGGCAGTTTGTGGCGGATCTTCGCCCATAATTTACGCGGCACGCACTTCGCCATGCGCTGTGCGTTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCttt                                                                                                                                                                                                      <  2:196242/251‑1 (MQ=255)                                                 tAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGATCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTcc                                                                                                                                                                                                    >  1:204946/1‑251 (MQ=255)                                                 catactcGCCTTCATGCT‑GCACCCAAGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTGCCAGAGCTTCAAAGGCGTTACTTACTGCCGCAAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGAGGCACGCACCTCGCCATGCGCTGTGCCGTGCGGCAGCTCGCTGAAGCGATAGCCGGTATGCGCTGGCAGGCTTTcc                                                                                                                                                                                                    <  1:348907/245‑1 (MQ=255)                                                   accacGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACACCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCTCGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCAc                                                                                                                                                                                                  <  2:297086/249‑1 (MQ=255)                                                   aaCACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCAc                                                                                                                                                                                                  >  2:119598/1‑251 (MQ=255)                                                            tCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGc                                                                                                                                                                                         >  2:267275/1‑251 (MQ=255)                                                                   cGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCg                                                                                                                                                                                  >  1:409283/1‑251 (MQ=255)                                                                   cGC‑CCCACGACGTGGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCGGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGTTTTGGc                                                                                                                                                                                   <  2:179987/250‑1 (MQ=255)                                                                        ccACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCCGGCTTTCCACGCCCCAGGCTTTGGCGAAtt                                                                                                                                                                              >  1:284003/1‑251 (MQ=255)                                                                               gTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTcgcg                                                                                                                                                                       >  2:115767/1‑251 (MQ=255)                                                                                ttGACGCTAAAATCACATCCGCCGCCGCCGCGTTTTTGGTCATAAGGATGTCCTGAACGATAACCAGTTCCCGATCTTCAAAGGCTTTACGTGCTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATTTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGTCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTcgc                                                                                                                                                                        <  1:151675/249‑1 (MQ=255)                                                                                     ggTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATATCCGGTAGGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGc                                                                                                                                                                 <  1:26581/251‑1 (MQ=255)                                                                                       tAAAATAACATCCGCCGCCGCCGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCg                                                                                                                                                               >  2:271935/1‑251 (MQ=255)                                                                                        aaaaTACCATCCGCCGACGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCCGTTCACGATCTTCAAAGGCTTTACGTACTGCCGACATCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGAGGCGATAGCCGGTATGCGCTGGCAGGCGTTCCACGCCCCAGGCTTTGGCGAATTTATCGCGGTTAGCCg                                                                                                                                                               <  2:380986/250‑1 (MQ=255)                                                                                          aaTAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGAt                                                                                                                                                            <  1:16939/251‑1 (MQ=255)                                                                                           aTAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATc                                                                                                                                                           <  1:230649/251‑1 (MQ=255)                                                                                             aaCATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTCCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCtt                                                                                                                                                         <  1:158276/251‑1 (MQ=255)                                                                                               cATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTc                                                                                                                                                       >  1:352059/1‑251 (MQ=255)                                                                                                      cgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                                                                <  1:115767/251‑1 (MQ=255)                                                                                                       gccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                                                               >  1:125910/1‑251 (MQ=255)                                                                                                           aCGCGGTATTGGTCATAAAGCTGACCTGAACGATAACCAGTTCCAGATCTTCAAAGGATTTCCGTACTGCCGACAGCTCCACGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGATGCGGCAGCTCGCTGATGCGATCGCCGGGATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGCATTTCTCGCGGTTAGCCGGATCTTTCACGTCCTGGTAt                                                                                                                                           >  2:462095/1‑251 (MQ=255)                                                                                                               ggTTTTGGTCATAAAGATGTCCTGATCGATAACCAATTCCAGCTCTTCAAAGGCTTTACGTACTGCAGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGACCATAATGTACGCGGCACGCAATTAGCCATGCGCTGGGCGGGGCGGCAGCTCGCTGATGCGATAGCCGGCATGCGCTGGCAGGCTTTACACGCCCCAGGCTTTGGCGAATTTCTCGCAGTTCGCCGGATCTTTCCCGCACTGGTATCCCg                                                                                                                                       >  2:185880/1‑251 (MQ=255)                                                                                                                gTTTTTGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCgg                                                                                                                                      <  2:360518/251‑1 (MQ=255)                                                                                                                                 gTCCTGAACGATAACAAGCTCCCGCTCTTCAAAGGCTTTACGTACTGCCGACAGCTCAGCGTCCGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGAACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGAGATAGCCGGTCTGCGCTGGCAGGCTTTCCACGCCCCAGGATTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCcgcg                                                                                                                     >  2:299120/1‑251 (MQ=255)                                                                                                                                   ccTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcg                                                                                                                   <  1:354679/251‑1 (MQ=255)                                                                                                                                    cTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcgc                                                                                                                  >  1:250866/1‑251 (MQ=255)                                                                                                                                      gAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGccc                                                                                                                <  1:271935/251‑1 (MQ=255)                                                                                                                                        aCGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGCGCGGCAGCTCGCTGATGCGATCGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCat                                                                                                              >  2:59679/1‑251 (MQ=255)                                                                                                                                             aaCCAGTTCCAGATCATCAATGGCTTGACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATACTGTACGCGGCACGCACTTCGCCATGCGCTTTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCg                                                                                                          <  1:245805/250‑1 (MQ=255)                                                                                                                                             aaCCAGTCCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTAGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGCACTGGTATCCCGGATACGTATCCGTCCACGCGCCCATATCGc                                                                                                         >  2:53600/1‑251 (MQ=255)                                                                                                                                              aCCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCa                                                                                                        <  1:63784/251‑1 (MQ=255)                                                                                                                                               ccAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGAGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGc                                                                                                         >  2:236052/1‑249 (MQ=255)                                                                                                                                                  gTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCa                                                                                                    <  1:378814/251‑1 (MQ=255)                                                                                                                                                             tCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAAc                                                                                            <  1:56246/248‑1 (MQ=255)                                                                                                                                                                 aGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTAGCCCATAATGCACGCGACACGCACTTCGCCATGCGCTGTGCGGAGCGGCAGCTCGCTGATGCGATAGCCGGGATGCGCTGGCCGGCTATCCACGCCCCCGGCTTTAGCGAATTTCTCGCAGTTAGCCGGGTCTTTCACGAACTGGAATCCCGGATCCGGATCCTGCAGCGCGCCCATATCGCAGGCACCCCGAActgtgttc                                                                                     >  2:119132/1‑244 (MQ=255)                                                                                                                                                                     tttACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAc                                                                                 >  1:370404/1‑251 (MQ=255)                                                                                                                                                                     tttACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAc                                                                                 >  1:169272/1‑251 (MQ=255)                                                                                                                                                                        aCGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCCCGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATAGCGCAGGCACCCTGAACGTTGTTCTGACCAc                                                                              <  2:344545/251‑1 (MQ=255)                                                                                                                                                                         cGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATCATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGAAGGCACCCTGAACGTTGTTCTGACCACg                                                                             >  2:195081/1‑251 (MQ=255)                                                                                                                                                                          gTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGACCGTTGTTCTGAccccg                                                                             >  2:147204/1‑247 (MQ=255)                                                                                                                                                                                 cGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGt                                                                     <  2:148095/251‑1 (MQ=255)                                                                                                                                                                                  gACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCCGGCTTTGGCGAATTTCTCGCGGATAGCCGGCTCTTTGACGTACTGGTATCCCGGATACGTATCCGGCAGCGGGCCCATATCGAAGGCACCCTGAACGTTGTTCTACCCACGAACCGTGtt                                                                    >  2:245776/1‑251 (MQ=255)                                                                                                                                                                                    cAGCTCCGCGTCAGGTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGAGGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATACCGCAGGCACCCGGAACGGTGTTCTGACCACGAACCGGGTTaa                                                                  <  2:329284/251‑1 (MQ=255)                                                                                                                                                                                      gCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAaca                                                                <  1:427782/251‑1 (MQ=255)                                                                                                                                                                                           gcgTCAGCTTGAAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCCCGAACCGGGTTAACACCCGc                                                           >  2:74052/1‑251 (MQ=255)                                                                                                                                                                                             gTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCAt                                                         >  1:166668/1‑251 (MQ=255)                                                                                                                                                                                              tccgTTTGTAGAGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGACTTTCTCGCGGTTCGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGGTGTTCTGACCACGAACCGGGTTCACCCCCGCATg                                                        >  2:166803/4‑251 (MQ=255)                                                                                                                                                                                               cAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGc                                                       <  2:425539/251‑1 (MQ=255)                                                                                                                                                                                                   ttGTAGCGGATCTTCGCCCATAATGTACGCGGCCCGCTCTTCGCCAGGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCATGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCTCCCTGAACGTTGTTCTGACCGCGAACCGGGTTAACACCCGCATGCgcc                                                    <  1:264085/250‑3 (MQ=255)                                                                                                                                                                                                     gTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCtt                                                  <  2:124945/250‑1 (MQ=255)                                                                                                                                                                                                       aGCGGATCTTCGCCCATAATGTACGCGGCACGCCCTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAc                                                <  2:250866/250‑1 (MQ=255)                                                                                                                                                                                                       aGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc                                               >  1:353733/1‑251 (MQ=255)                                                                                                                                                                                                                tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCCGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCCGGTTAACACCCGCCTGCCGCTTACCGAGGttccc                                      >  2:17274/1‑248 (MQ=255)                                                                                                                                                                                                                       tAATGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAACCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGc                               >  2:334790/1‑251 (MQ=255)                                                                                                                                                                                                                       aaaggaaCGCGGCACGCCTTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATATCCGGTAGGCGCTGGCAGGCTTTCAACGACCCAGGCTTTGGCTAAATTCTCGCTGTTAGCCGGATTTTTCACTGACTGGTATCCCGGATACGTATCCGGCATCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCAGGTTAACACCCGCATGCGGATTACCGATGTTACCGGTCAGc                               <  2:462924/245‑1 (MQ=255)                                                                                                                                                                                                                        aaTGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGAGCGACAGCTCGCTGATGCGATAGCCGGTATGCGATGGCCGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTCCTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGc                               >  2:226441/1‑250 (MQ=255)                                                                                                                                                                                                                         atttACGCGGCACGCTCTGCGCCATGCGCTGTGGTGTTCTGGAGCTCTCTGAGGCGATCGTCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCTGATACGTATCCGGCAGCGCCGCCATAGCGCCGGCACCCTCAACGTTGTTCTGACCACGAACAGGGTTAACACCCGCATGCGTCTTACCGAGGTTACCgcgtagca                              <  2:297721/247‑8 (MQ=255)                                                                                                                                                                                                                         aTGTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGTGGTTACCGGTCAGCac                             >  2:160816/1‑250 (MQ=255)                                                                                                                                                                                                                           gTACGCGGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATc                            <  2:125910/250‑1 (MQ=255)                                                                                                                                                                                                                              cgcgGCACGCACTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGACAGCCGGTATGCGCTGGCAGGCCTTCCACGCCCCAGGCTTTGGCGAATTTCTAGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATcgcg                         >  1:220547/1‑250 (MQ=255)                                                                                                                                                                                                                                       cacTTCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTc                >  2:38237/1‑250 (MQ=255)                                                                                                                                                                                                                                          ttCGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGCTGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGTCTTACCGCGGTTACCGGTCAGCATCGCGAGGCTGGTCag              >  1:13288/1‑249 (MQ=255)                                                                                                                                                                                                                                            cGCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGGCAgaga           >  2:4547/1‑250 (MQ=255)                                                                                                                                                                                                                                             gCCATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGCGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAAc         >  2:330639/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACg        >  1:360199/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccATGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACg        <  1:399784/251‑1 (MQ=255)                                                                                                                                                                                                                                                aTGCGCTGTGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGc       >  1:225632/1‑250 (MQ=255)                                                                                                                                                                                                                                                aTGCGCTGTGCGGTGCGGAAGCTCGCTGATGCGATCGCCGGGATGCGCTGGCAGGCTTTCCACGCACCAGGCTTTGGAGAATTTCTCGAGGCTAGCCGGATCTTTCACGTACTGGAATCCCGGATACGTATCCGGCAGCGCGCCCATACCGCAGGAACCCTGAACGTTGTTCTGACCACGACCCGGGTTAACAACCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGCGGCTGGTCAGAGAACGcc      >  2:466591/1‑250 (MQ=255)                                                                                                                                                                                                                                                  gcgctgggcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCGGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCAc     <  2:169272/250‑1 (MQ=255)                                                                                                                                                                                                                                                    gctgtgcGGTGCGTCAGCGCGCTGAGGCGATAGCCTGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGTTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGt  <  2:284998/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       TCGGTTCAACCGCCTTGAAGAAACGCTGGAAGCCACGGTCAGCCGCAGTAAACACGCCTTCATGCTCGC‑CCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGT  >  NC_000913/4297953‑4298445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑