Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,286,033 C→T A447T (GCG→ACG)  acs ← acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,286,0330CT95.0% 222.6 / NA 82A447T (GCG→ACG) acsacetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base T (41/35);  minor base G (0/4);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 5.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCC  >  NC_000913/4285786‑4286274
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACAAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCgg                                                                                                                                                                                                                                                 >  1:19180/1‑251 (MQ=255)
   cccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑g                                                                                                                                                                                                                                             <  1:320667/251‑1 (MQ=255)
         cGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGcc                                                                                                                                                                                                                                       >  1:409906/1‑251 (MQ=255)
              aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCga                                                                                                                                                                                                                                     >  1:208396/1‑248 (MQ=255)
                 ggAGACGTTCAGCACGTCCTCCACACGCCCGGGTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACTCCGAAGAACg                                                                                                                                                                                                                               <  2:179180/251‑1 (MQ=255)
                  gagaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGCAGAACgg                                                                                                                                                                                                                              >  1:68450/1‑251 (MQ=255)
                    gaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATATCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTCCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGAc                                                                                                                                                                                                                            <  2:39912/251‑1 (MQ=255)
                    gaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGGCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGAc                                                                                                                                                                                                                            <  2:349813/251‑1 (MQ=255)
                     aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACg                                                                                                                                                                                                                           <  2:10248/251‑1 (MQ=255)
                      cGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACgt                                                                                                                                                                                                                          <  2:325481/251‑1 (MQ=255)
                           aGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCTCGCCGTCGCCGCTGAAATACATAGTTTTGAAGGTGGAGAAGTAGGGCTGTTCAAAACGGTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGTCGATTACCAGGCGACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATTGACCAGCGTCGGTT‑GCACGCCGAATAACTAACGTGTTGc                                                                                                                                                                                                                     <  2:301905/251‑1 (MQ=255)
                              ccgtcgtACACTCGCCCGGTTATCCAGTAATAGCCGTCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAGCGCCAAACAGCGTACGCGCCTTACCCGGCCAGGAGTCGGTGTTTACCAGTCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTAGCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGa                                                                                                                                                                                                                  <  2:415123/250‑1 (MQ=255)
                                gtcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGTCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCATGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAAc                                                                                                                                                                                                                <  2:166090/251‑1 (MQ=255)
                                gtcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAAc                                                                                                                                                                                                                >  1:439759/1‑251 (MQ=255)
                                  cgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAAcc                                                                                                                                                                                                               <  1:419119/250‑1 (MQ=255)
                                   gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCATCGCCGCTGAAATACATATTTTTGAAGGAGGAGAAGTCGGTCTGTTCAAAACGTACGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCACCCAAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑TCACGCCGAAGAACGGACGGGTTGCCGCACCgg                                                                                                                                                                                                             >  2:427474/1‑251 (MQ=255)
                                      cacgcGCCCGGTTAGCCAGTAATAGCCATCTTCATCGCAACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGTTTACCTTCGTTATCGACCATCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCtt                                                                                                                                                                                                          <  2:49470/247‑1 (MQ=255)
                                          cGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTTAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGACCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTCCCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGGT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAg                                                                                                                                                                                                      <  2:329039/251‑1 (MQ=255)
                                             ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTTATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTCCCGTCGGTGGCCCCTTCCAGCTGGTTACCTTCGTTATCGACCATCGGCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTc                                                                                                                                                                                                   <  1:270094/251‑1 (MQ=255)
                                              cGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCTCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCg                                                                                                                                                                                                  <  2:335251/251‑1 (MQ=255)
                                               ggTTAGCCAGTAATAGCCATCTTCAGCGCTACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAGCAGCGTACGCGCCTTACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGTGGTTTCCCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGGTGCCGAATCGGCTTTCAGCtggg                                                                                                                                                                                                 <  2:385563/251‑4 (MQ=255)
                                                   aTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgc                                                                                                                                                                                             >  1:238485/1‑251 (MQ=255)
                                                      cAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCCGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGCCCAGCGTCGGTT‑GCACGCCGCAGAACGGCCGTGTTGCCGACCCGGCTTTCCGCTCGGTAGCGcc                                                                                                                                                                                          >  1:166271/1‑251 (MQ=255)
                                                       aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGccc                                                                                                                                                                                         >  2:32160/1‑250 (MQ=255)
                                                       aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCa                                                                                                                                                                                         <  1:366102/251‑1 (MQ=255)
                                                            tAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGCGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGCAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGc                                                                                                                                                                                      >  2:322350/1‑249 (MQ=255)
                                                                    ttCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAACACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGGACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAgaccgaggggtgg                                                                                                                                                                            >  2:374445/1‑239 (MQ=255)
                                                                            gACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGaaa                                                                                                                                                                    <  1:414060/251‑1 (MQ=255)
                                                                                 gcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCCCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCCGGCAGCGGGGTGATCATGAAAccgcc                                                                                                                                                               >  2:386873/1‑251 (MQ=255)
                                                                                   gcctccgccgcTGAAATACATATTTTTGAAGGTGGAGAAGGAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTCCGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTCCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGGGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCgg                                                                                                                                                             <  2:412640/246‑1 (MQ=255)
                                                                                   gcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCgg                                                                                                                                                             <  1:215720/251‑1 (MQ=255)
                                                                                   gcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGGTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTGGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTTATCATGAAGCCGCCgg                                                                                                                                                             <  2:180232/251‑1 (MQ=255)
                                                                                     cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGtt                                                                                                                                                           >  1:18107/1‑251 (MQ=255)
                                                                                        cgccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTAAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAACGTCGGTT‑GCACGCCGAAGAACCGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGCAACAGCCGGTATCg                                                                                                                                                        >  1:236894/1‑251 (MQ=255)
                                                                                            gcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCt                                                                                                                                                    >  1:291306/1‑251 (MQ=255)
                                                                                               gAAATACATATTTTTGAAGGTGGATAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCTGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACTCCgaag                                                                                                                                                                                                                                   <  2:59560/169‑1 (MQ=255)
                                                                                                aaaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTACAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCGGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTGGGAAGCGCAAGGCAGCGGGGGGCTAAGGAAACCGCCGGGTTCggacggcc                                                                                                                                                 >  1:318443/1‑244 (MQ=255)
                                                                                                  aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccacc                                                                                                                                              >  1:417569/1‑251 (MQ=255)
                                                                                                              gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCAAGCAACCGCCGGTTTCGGTCTGCCACCAGGAATCgcccc                                                                                                                                  >  2:466284/1‑247 (MQ=255)
                                                                                                               aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGATCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGaccac                                                                                                                                 >  1:251137/1‑251 (MQ=255)
                                                                                                                 ggTGGAGAATTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGAACGCGCCTGACCCGGCCAGGAGTCGGTGATTCCCCGGCTACCTTGGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGTTT‑GCACGCCGAAGAACGGACGTGTCGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGAGCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCg                                                                                                                               <  1:265771/251‑1 (MQ=255)
                                                                                                                             ggTCTGTTCAAACCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGt                                                                                                                   >  1:153226/1‑251 (MQ=255)
                                                                                                                                 tGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCACGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGACCGGACGTGTTGCCGAACCGGCCTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGACGGCTTCGGTCTGCCACCAGGAATCGACCACCGGGCATTTCTCGTTGcc                                                                                                               >  1:443821/1‑251 (MQ=255)
                                                                                                                                  gTTCAAAACGTTCGTGACCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGGATCGACCACCGGACCTTTCTCGTTGCCg                                                                                                              >  2:266353/1‑251 (MQ=255)
                                                                                                                                    tCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAt                                                                                                            >  2:259775/1‑251 (MQ=255)
                                                                                                                                     caaacctttcgggagcGCCAAACAGCGGACGCCCCTGACCCGGCCAGGAGTCGGTGATTGCCTGGCTCCCTGCGGTTGCCCCCGCCAGCGGTTTCCCTTCGTTACCGCCCAGGGGCGGTT‑GCACGCCGAAGACCGGCCGTGTTCCCAAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGGGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTGGCCGAt                                                                                                            <  2:417569/235‑1 (MQ=255)
                                                                                                                                     aaaaacggtggtaagcGCCAAACAGCGTACGCGCCGGACCCGGCCAGGAGCCGGTGATTCCCAGGCTACCTTCGGTGGCCCCCCCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCCCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtt                                                                                                           <  1:381589/236‑1 (MQ=255)
                                                                                                                                        aaCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGGCTGCCCCCAGGTATCGACCACCGGACATTTCTCGTTGCCGAttttt                                                                                                        >  2:325592/1‑251 (MQ=255)
                                                                                                                                          cGTTCGTCATCGCAAAACAGCGAACGCGCCTGACCCGGCCACGAGTCGGAGCTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTCTCGCCCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGATTCGGTCTGCCACCCGGTATCGACCACCGGACATTTCTCGTTGCCGAttttttt                                                                                                      >  2:309605/1‑251 (MQ=255)
                                                                                                                                           gTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCCCCGGACATTTCTCGTTGCCGAtttttttt                                                                                                     >  2:355507/1‑250 (MQ=255)
                                                                                                                                           gTTCGTGATCGCCAAACAGCGTACGCGCCGGACCCGGCCAGGAGTCGGGGATTACCAGGCTACCTTCGTTGGCCCCCTCCAGGGGGTTACCTTCGTAATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCGAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCg                                                                                                                                      <  1:272757/218‑1 (MQ=255)
                                                                                                                                               gTGATCGCCAAACAGCTTACGCGCCTGCCCCGGCAAGGGTTCGGGGATTACCAGGCTCCCTCCGGGGGCCCCCCCCAGCGGGTTACCTTCGTTATCTACCAGCGGCGGTT‑GCACGCCGAAGAACGGCCGTGTTGCCGACCCGGTTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGt                                                                                                 <  2:80526/251‑1 (MQ=255)
                                                                                                                                                    cGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCAc                                                                                            >  2:297983/1‑251 (MQ=255)
                                                                                                                                                        aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGCCGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCATTACCACTccc                                                                                        >  2:273880/1‑251 (MQ=255)
                                                                                                                                                         aaCAGCGAACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCAACCTTCGGTGGCCCCCTCACGCGGGTTACCTTCGATATCGACCAGCGTCGGTT‑GCACGACGAAGAACGGACGTGTTGCCGAACCGGCTTTCCGCTAGGTCACGCCAGGCAGCGGGGTGATCATGAACACGCCGGTTTCGGTCTGCCACCACGTATCGACCCCCGGACATTTCCCGTTGCCGATTATTTTCCAGTCCCACTccc                                                                                        >  1:300793/1‑250 (MQ=255)
                                                                                                                                                          aCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCAc                                                                                      >  1:113132/1‑251 (MQ=255)
                                                                                                                                                          aCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCAc                                                                                      >  2:113913/1‑251 (MQ=255)
                                                                                                                                                              cGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGTACCACTCCCACGCtt                                                                                  >  1:187199/1‑251 (MQ=255)
                                                                                                                                                              cGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCtt                                                                                  >  1:361421/1‑251 (MQ=255)
                                                                                                                                                               gTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTc                                                                                 <  1:212658/251‑1 (MQ=255)
                                                                                                                                                               gTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCAAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCCGGTATCGACCACCGGACATTTCTCGTTGCCGGTTTTTTTCCAGTAACACTCCCCCGCTTc                                                                                 >  2:459595/1‑251 (MQ=255)
                                                                                                                                                                  cgcgCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCgg                                                                              >  1:143613/1‑251 (MQ=255)
                                                                                                                                                                        tGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCAGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAAt                                                                        >  1:184212/1‑251 (MQ=255)
                                                                                                                                                                         gACCCGGCCAGGAGTCGGTGATTCCCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTCCCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTaa                                                                         <  1:41454/249‑1 (MQ=255)
                                                                                                                                                                            ccGGCCAGGAGTAGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTAGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAACGCCGGTTTCGGGCTGCCACCAGGTATCGACCACCGGACCTTTCTCGGTGACGCTTTTTTTCCAGTACAACTCCCACGCATCCGGGTTAATTGGc                                                                    >  2:402411/1‑251 (MQ=255)
                                                                                                                                                                                    gAGTCGGTTATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCAc                                                            <  1:374445/251‑1 (MQ=255)
                                                                                                                                                                                        cGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCG‑CTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCCCGCCCACGGAAc                                                       >  2:361022/1‑251 (MQ=255)
                                                                                                                                                                                         ggtgatacccaggcgtcctgcgggggCCCCCTCCAGCGGGTTACCTTCGTTACCGACCAGCGTCGGTT‑GCCCGCCGAAGACCGAACGTGTTGCAGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATAATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAAc                                                       <  1:384002/227‑1 (MQ=255)
                                                                                                                                                                                                      cTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTCTTGCCGCACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAACCCGCCGCCTTCGGTCTGCCCCCAGGTATCGACCACCGGGCATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGCGTTAATTGGCTCGCCCAAGGCACCGAGAATgcgc                                            >  1:297059/1‑249 (MQ=255)
                                                                                                                                                                                                        cccTTCGGTGGCCCCCCCCAGGGGGTTCCCTTCGTTATCGACCAGCGTCGGTG‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGTAAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGcg                                        <  1:302096/250‑1 (MQ=255)
                                                                                                                                                                                                        aCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGc                                         <  1:159377/250‑1 (MQ=255)
                                                                                                                                                                                                            tCGGTGGCCCCCTCCAGCGGGTTCCTTTCGTTATCGCCCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGcgacg                                     <  1:400932/250‑1 (MQ=255)
                                                                                                                                                                                                                gggCCCCCTCCTGCGGTTTACCTTCGTGATCGCCCAGCGCCGGTG‑GCCCGCCGAAAAACGGCTGGGTTGCCGACCCGGCTTTCATCTCGGGAGCTCCAGGTTGCGGGGTGTGCACGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACAGTTTTCGTTGCCGATTTTTTGCCAGTACCACTCCGACGCTTCCGGGTTAATTGGCTCGCCCTCTGAACCGAGAATGCGCAGCGATGAAcg                                 <  2:305865/249‑1 (MQ=255)
                                                                                                                                                                                                                  gCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAAcggtc                              <  1:14317/251‑1 (MQ=255)
                                                                                                                                                                                                                  ccccccTCCTGCGGTTTCCGTTCGTTATCGACCACCGTCGGTT‑GCCCGCCGAAGAACGGCCGTGGGGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATAAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACGCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAAcggtc                              <  2:424392/250‑1 (MQ=255)
                                                                                                                                                                                                                      ccTCCAGCGGTTTACCTTCGTTATCGACCAGCGTCGGTT‑GCCCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTg                          <  1:355507/251‑1 (MQ=255)
                                                                                                                                                                                                                              gggtgtccTTCGTTATCGGCCGGCGGCGGTT‑GCACGCCAAAGATCGGACGTGTTGCCGAACCGGCTTTCAGCTCGTTAGCGCCAGGCAGCGGGGGGATTATGAAACCGCCGGTTTCGGTCTGCCACCAGGGATCGACCACCGGACATTTCGCGGTGCCGATGTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGAt                  <  1:460971/245‑1 (MQ=255)
                                                                                                                                                                                                                                          ttATCGCCCAGCGTCCGTT‑GCACCACGAAGCACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGAAGCGCACGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGCATCGCCCACCGGACATTTCTCGGTGCCGGTTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGATAGCCCACGGAACAGAGAAGACGCAGCGACGAACGGTCGGTGACTTCGATCGCTTTATCGcc      >  2:285503/1‑251 (MQ=255)
                                                                                                                                                                                                                                           taccgtccAGCGTCGGTTAGCA‑GCCTAAGATCGGTCGTGTTGCCGACCCGTCTTTCCGCTCGGTATCGCCAGGCAGCGGGTTGATCATGAAACCGCCGGTTTCGTTCTGCCACCAGGTATCTACCACCGTACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAAATGGCTCTCCCACGGAGCCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGcc      <  1:51069/244‑1 (MQ=255)
                                                                                                                                                                                                                                           tATCGACCAGCGTCGGTT‑GCCCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGAAGCGCCAGGCAGCGGGGTGATCATGAAACCGACGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCCCGGAACCGAGCAAGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCt     >  2:399411/1‑251 (MQ=255)
                                                                                                                                                                                                                                             gcGACCAGCGTCGGTT‑GCACGCCGAGGAACGGACGTGTGGCCGACCCGGTTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGCTCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTc   <  2:393216/250‑1 (MQ=255)
                                                                                                                                                                                                                                              cGACCAGCGTCGTTG‑GCACGCCGAAGACCGGACGTGTTGCCGAACCGGCTTTCAGCCCGGTGGCGCCAGGCAGGGGGGTGATCAGGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTcc  <  1:448154/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
GTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTT‑GCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCC  >  NC_000913/4285786‑4286274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑