Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,284,077 C→T A276T (GCG→ACG)  actP ← acetate/glycolate:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,284,0770CT98.0% 161.6 / NA 51A276T (GCG→ACG) actPacetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (30/20);  minor base A (1/0);  total (31/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTAC  >  NC_000913/4283830‑4284321
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
ccGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCcgtcgt                                                                                                                                                                                                                                                   <  1:115562/251‑1 (MQ=255)
     gAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCa                                                                                                                                                                                                                                               >  1:281942/1‑250 (MQ=255)
        cAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAAc                                                                                                                                                                                                                                            >  1:231823/1‑250 (MQ=255)
           gTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATc                                                                                                                                                                                                                                         >  1:417533/1‑250 (MQ=255)
                          aTTCGCCAGTTGAACGGCCTCCAGTTTGTCACCACCAAGCAGATGGACCTCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCTATAATAAAGGTCAGAATATAGAATTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTGCGCGGGCATCGCTTACGGTAAAGAAGCGCATCTGAATGTGCGGCAAGGTCGTGGTACCAAACAGCCGTCCCGGACCCagag                                                                                                                                                                                                                         <  2:280708/251‑1 (MQ=255)
                          aTTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCACCGCGTCTTTATATTCCGGCTTCGCCACAACCAGCATGATCGCGCCGCAGCCGATAATAAAGGTCAGAATATAGAAGAAGCCCATAAACCCGGTGGCGTAGAAAACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGACGCGCATCAGAATGTGCGGCAAGCCCGAAGTACCAAACATCAGTcccagaccccg                                                                                                                                                                                                                           >  2:449497/1‑247 (MQ=255)
                            gcgccggggtacctgccgccATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCTTACCAAACATCAGTCCCAGATCCagaga                                                                                                                                                                                                                        <  2:273364/236‑1 (MQ=255)
                                cAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAgcg                                                                                                                                                                                                                   >  2:80081/1‑251 (MQ=255)
                                          gccgccATGTTGTAACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCgg                                                                                                                                                                                                                >  1:351818/1‑244 (MQ=255)
                                          cccgccAGGTAGTGACCACAAATCAGATGGCCCGGCGCGTCTTTATATTCCAGATTCGCACCAACCATCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTTTCGTAGACCTCGCAGTTACGTGCATCGCGGCCATCGCTGGCTGTAAAGAAGCGCAGCAGAATGTGCTGCAAGCCCGTCGTACCAAACATTAGTCCCAGACCCAGAGAGAGCTCGGAGATCgg                                                                                                                                                                                                         <  2:167787/250‑1 (MQ=255)
                                                    tgttACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCAcc                                                                                                                                                                                               >  1:48537/1‑251 (MQ=255)
                                                        ccccccaATCAGAGGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAgc                                                                                                                                                                                            <  2:86434/246‑1 (MQ=255)
                                                                  aGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGCAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCtt                                                                                                                                                                                 >  2:145517/1‑251 (MQ=255)
                                                                   gATGGACCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCGTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTc                                                                                                                                                                                <  2:342018/251‑1 (MQ=255)
                                                                      ggcccgaagagTCTTTATCTTACGGATTCGCACCAACCAGCATGATCGCTCCGAAGCCCATAATAAAGGAAAGAATATCGACGTAGCCCATAAACCCCGTGGCGTAGCACCCGCTCTTACGTGCTTCGCGTGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCACGCCCGTCGTCCCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGCATCTTTCCCCAGCCCGCCCAGCTCCatg                                                                                                                                                                             >  2:417942/11‑251 (MQ=255)
                                                                           gccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTc                                                                                                                                                                        <  1:80081/251‑1 (MQ=255)
                                                                                      atatTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATCAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGCGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCg                                                                                                                                                             >  1:234776/1‑251 (MQ=255)
                                                                                      atatTCCGGATTCGCACCAACCAGCATGATAGCGCCGAAGCCGATAATAACGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAACCCGCCCGGCTTCATGATGTCGTCACCTTTCg                                                                                                                                                             >  1:367705/1‑251 (MQ=255)
                                                                                              gATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTAcc                                                                                                                                                     <  1:431722/251‑1 (MQ=255)
                                                                                                  cGCACCAACCAGCATGATCGCGCCGAAGCCGATAATCAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGcc                                                                                                                                                  >  2:291993/1‑250 (MQ=255)
                                                                                                     accaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGCTCGGATCTTTCACCAGCCCGCCCTGCTTCATGATGTCGCCACCTTTCGGGTGCACCGCCATCg                                                                                                                                              >  2:335856/1‑251 (MQ=255)
                                                                                                          ccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTGCGGGTGTACCGCCATCGCTTCa                                                                                                                                         <  2:281942/251‑1 (MQ=255)
                                                                                                                                       aaGGTCAGAATACAGAAGTAGAACATAAACCCGGTGGAGTACAACACGATCATACGTGCTTCGCGGGCATCGCTGACTGTACAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGTGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATCATGTCGCCACCTTTCGGGTGTACCGCCATCGCTTCACTGATCAGATTGTTGAAGCTACAGTCGAc                                                                                                            >  2:105498/1‑251 (MQ=255)
                                                                                                                                        aGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACg                                                                                                           >  1:309772/1‑251 (MQ=255)
                                                                                                                                              taaTATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGATGACTGTAAAGAAGCGCATCAGAATGGGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGGTCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGGGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtc                                                                                                     >  1:461353/2‑251 (MQ=255)
                                                                                                                                                atatAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGGGTTtcat                                                                                                   >  1:266818/1‑251 (MQ=255)
                                                                                                                                                  agaGAATTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACATATTGTTGAAGCTAAAGCCGACGTGTTtcatca                                                                                                 <  1:400125/249‑1 (MQ=255)
                                                                                                                                                          aGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACAAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTAGACACCTTTCGGGTGTACCACCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGAGTTTCATCACCATaa                                                                                           >  1:155398/1‑249 (MQ=255)
                                                                                                                                                                 aaaCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATa                                                                                   <  2:231823/250‑1 (MQ=255)
                                                                                                                                                                   aCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATaa                                                                                  >  1:129126/1‑249 (MQ=255)
                                                                                                                                                                       ggTGGCTTCGAACACGCTCTTCCGTGCGTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGCATGTGCGGCAAGCACGTCGTACCAATCACCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTGCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTgg                                                                            <  2:111418/251‑1 (MQ=255)
                                                                                                                                                                         gggCGTAGAACACGCTCTGACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGATAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCAGGATGTCGACAGCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCTTAAAGGTGGCa                                                                          <  2:241845/250‑1 (MQ=255)
                                                                                                                                                                               aGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaac                                                                    >  2:189917/1‑251 (MQ=255)
                                                                                                                                                                                      gCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaacagcaaca                                                             <  2:170923/251‑1 (MQ=255)
                                                                                                                                                                                           tACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACg                                                        >  1:155326/1‑251 (MQ=255)
                                                                                                                                                                                              gTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCt                                                     <  1:96373/251‑1 (MQ=255)
                                                                                                                                                                                                gCTTCGCTGGCAGCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCttt                                                   <  2:309772/251‑1 (MQ=255)
                                                                                                                                                                                                gCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCTCCGAACAGCAACAGCACGGCttt                                                   <  2:106482/251‑1 (MQ=255)
                                                                                                                                                                                                    cgcgGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGAt                                                >  2:416027/1‑250 (MQ=255)
                                                                                                                                                                                                       gggCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAAtt                                            >  1:133054/1‑251 (MQ=255)
                                                                                                                                                                                                         gCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTg                                          >  2:142934/1‑251 (MQ=255)
                                                                                                                                                                                                              gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACAGCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCTGCTTCATGATGTCGACACCTTTCGGTTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCCCCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAccc                                     <  1:141933/251‑1 (MQ=255)
                                                                                                                                                                                                                tGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAccc                                     >  1:430597/1‑249 (MQ=255)
                                                                                                                                                                                                                  aCTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAggt                                 <  2:129126/251‑1 (MQ=255)
                                                                                                                                                                                                                             gcgcATCAGAATGTGCGGCAAGCACGTCGTACCAAACATCAGTCCCAAACCCCGAGAGAGCGCGGAGATCGGCTCTTTAACCAGCCCGCCCGGCTTCATGATGGCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGACCATAAAGCTGGCACCGAACAGCAACAGCACGTCTTTGCTAATTTGCACCCACGTGGTCGCCAGcc                      >  2:42895/1‑250 (MQ=255)
                                                                                                                                                                                                                              cgcATCAGAAAGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGGCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCAGAAAGGCCATAAAGCTTGCACCTAACAGCAACAGCACGCCTTTGATAATTTGCACCCAGGTGGTCGCCAGCAt                     <  2:446127/251‑1 (MQ=255)
                                                                                                                                                                                                                                  tCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTAGTCGCCAGCATgccg                 >  1:221264/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAGAGCTGGCACCGAACAGCAACATCGCGGCTGTGATAATTTGCTCCCAGGTGGTCGTCAGCATgccgcc               <  2:234776/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATgccgcc               >  1:291931/1‑251 (MQ=255)
                                                                                                                                                                                                                                         gtgCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAAc           >  1:18379/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAgg        >  2:462971/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 aGCCCGTCGGACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTAc  >  1:96265/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
CCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTAC  >  NC_000913/4283830‑4284321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑