Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,278,488 C→T P4S (CCA→TCA)  ghxP → guanine/hypoxanthine transporter GhxP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,278,4880CT97.0% 110.8 / NA 33P4S (CCA→TCA) ghxPguanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/14);  minor base G (0/1);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTG  >  NC_000913/4278245‑4278727
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                
gTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAgc                                                                                                                                                                                                                                           <  1:89598/251‑1 (MQ=255)
  cAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAAGTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAAGTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAgcg                                                                                                                                                                                                                                          <  1:139191/250‑1 (MQ=255)
                    aaaaGCAGCAAACTTCAGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCAACGCATCTTGACGAACATTAAACTATAAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGAACCGGCGGGTCAccac                                                                                                                                                                                                                       >  2:90914/1‑248 (MQ=255)
                       agcagcAAACTCAGGTCGAAAAAGCCGCTATGTTCGCCGGCTAATCGTTTGCTTTTTTTACTACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCCGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCAACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGtttctttcact                                                                                                                                                                                                                    >  2:462972/1‑242 (MQ=255)
                             acaCTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATAGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGAATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCCGCGCGTACcggcgtgttgcaccacaccccgg                                                                                                                                                                                                              >  2:35537/3‑233 (MQ=255)
                                       tGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGCAACTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACttgttacctgggttttaatatt                                                                                                                                                                                                    >  2:256108/1‑230 (MQ=255)
                                           aaaGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGACGCCTGGTTTTAAATTT‑taca                                                                                                                                                                                                >  1:54188/1‑250 (MQ=255)
                                                              gATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCCGCACATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTTCA‑AACGTGGCAGCACttttcgtc                                                                                                                                                                            >  1:212198/1‑244 (MQ=255)
                                                                    cGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAg                                                                                                                                                                      <  1:156216/251‑1 (MQ=255)
                                                                       ttGCTTTTTTTACCACACGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCTCTCCACGCCTGGGTTTAAATTT‑CACAACGTGGAAGCACTTTCCGTCAGGCAGTgg                                                                                                                                                                   >  1:107897/1‑251 (MQ=255)
                                                                            ttttttACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGATCACTCGACGCCTGGTTTTAAATTT‑CACAACGTGGAAACACTGTCCGTCAGGGAGTGGTTGcc                                                                                                                                                              >  1:335884/1‑251 (MQ=255)
                                                                                ttACCACCCGTTTTGTATGCGCGGCGCTACACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACcg                                                                                                                                                                                                                                   >  2:387179/1‑179 (MQ=255)
                                                                                ttACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACcg                                                                                                                                                                                                                                   <  1:387179/179‑1 (MQ=255)
                                                                                ttACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACACATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGGCGCCCGGTGTAAAATTT‑TACAACGTGGGAGCAACGTACGTCAGGAAGGGGGTGCCgggg                                                                                                                                                          >  2:191492/1‑250 (MQ=255)
                                                                                  accaccCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGtt                                                                                                                                                         <  1:46670/250‑1 (MQ=255)
                                                                                          ttttGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTATCTCGCCGCCTGGTTTATAATTT‑CACAACGCGGAAGCACTCTCCGTCAGGGAGGGGGTGCCGGGGTTACAACGtt                                                                                                                                                >  2:365029/1‑251 (MQ=255)
                                                                                              gTATGCGCGGAGCTAAACGTTTGCGTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCAACAGCGCGTACCGGCGGTTCACTCGACGCCTGGTGTAAAATTT‑CACAACGGGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTCCTg                                                                                                                                            <  2:214882/251‑1 (MQ=255)
                                                                                                  gcgcGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGa                                                                                                                                        <  1:176099/251‑1 (MQ=255)
                                                                                                     cgGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATgg                                                                                                                                     <  1:391941/251‑1 (MQ=255)
                                                                                                        aGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCt                                                                                                                                  <  1:122247/251‑1 (MQ=255)
                                                                                                                gTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCCACGCCCGGTTTAAAATTT‑TACAACATGGGAGCACTGTCCGTCAGGAAGGTGTTGCCGGGTTAACAACCTTTCTGGCGATGGGCTACTccctc                                                                                                                          >  1:113751/1‑247 (MQ=255)
                                                                                                                gTTTGCTTTTTTGCGAAGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCAATCTTCGCCTTGGTTAAAATTT‑CTCAACGTGGAAGGAATGTCCGTCAGGAAGTGGTTGCCCGGTTtacaacacttcttgggaaggtcttcacggtc                                                                                                                          >  1:181859/1‑226 (MQ=255)
                                                                                                                 tttGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGGTTTAAATTT‑TACAACGTGGAAGCACTGTCCGGCAGGGAGTGGTTTCCGGGGTAACAACCTTTTTTGCGGTGGGCTACTCGGTcc                                                                                                                         >  1:78749/1‑250 (MQ=255)
                                                                                                                       tttttGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCAtcgt                                                                                                                     <  1:115697/249‑1 (MQ=255)
                                                                                                                                     caAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑TCCCACGTGGGAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCCGGGATGTTg                                                                                                     >  1:79830/1‑251 (MQ=255)
                                                                                                                                                  aCCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCAGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGGTCACTCGACGCCCGGGTTAAAATTT‑CACCACGAGGGAGCACTGTCCGTGTGGAAGTGGGTGCCTGGGTAACAACGTTTTTGGCGGTGGTGTACTCGGGCATCGTCGTTTCCGGGATGTTGGGTAAAGCAGGCt                                                                                        >  1:123060/1‑251 (MQ=255)
                                                                                                                                                        aGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGc                                                                                  <  1:86120/251‑1 (MQ=255)
                                                                                                                                                                gATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGTTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCa                                                                          <  1:402455/251‑1 (MQ=255)
                                                                                                                                                                               gCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCTCTGTCCGTCAGGAAGTGGTTGCCGGGTTAACATCGTTTCTGGTGATGGTCTCCTCGGTCATCGTCGTTGCAGGTAGTTTGGGTAAATGAGTCTTCCCGTGTGCGTCAGTTTTCGGTGCAAcc                                                           <  2:312071/251‑1 (MQ=255)
                                                                                                                                                                                cAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTTAAATTT‑TACAACGTGGAAGCACTGTCCGTCCGGAAGTGGTTTCCCGGGTAACAACGTTTTTGGCGATGGTCTACTCGGTCCTCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAAccc                                                          >  1:188686/1‑250 (MQ=255)
                                                                                                                                                                                  gggtaTTGTCCGCTACGCATCTTTACAAAAATTGAGCCCTCAGGGGATGTGTTCTGATTGCTACGGCATCGGCGCGTACCGGCGGTCCACTCGACGCCGGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGCCAGGAAGTGGTTGGCGGGTGAACAACGTTTCGGGCTATGGGCTTGGCAGTCGTCGTCGTTCCAGGTAGTTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGt                                                        <  1:234073/247‑1 (MQ=255)
                                                                                                                                                                                   gggATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTTAAATTT‑CACAAAGTGGAAGCACTTTCCGTCAGGAAGTGGTTTCCGGGGTAACAAAGTTTCTTGCGATGGTCTACTCCGGCATAGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGGTTTTGTTGCAAACTGtt                                                       >  1:310919/1‑250 (MQ=255)
                                                                                                                                                                                                                                         gaggTCTACGTCATCAGCGCTTACGGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGGCAGGAAGTGGTTGCCGGTTTAACAACGTTTCTGGCGATGGTCTGCTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCATTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTg  <  1:233771/247‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                
GTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTT‑CACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTG  >  NC_000913/4278245‑4278727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑