Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,271,130	C→T	E914E (GAG→GAA)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,271,130	0	C	T	100.0%	207.3 / NA	59	E914E (GAG→GAA)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/20);  total (39/20)

TGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGCTTCACCG  >  NC_000913/4270889‑4271376                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       tGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCgccgcc                                                                                                                                                                                                                                                >  1:246824/1‑250 (MQ=255)       aCCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGCGGATTTCGCCGCCACCACTg                                                                                                                                                                                                                                         >  2:402892/1‑251 (MQ=255)                  cGACGGTTCGCTGCCAGATTCGCAAATCGATTATCTGGTGGATGCGTTTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGGAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCGCAGAAAGCGGCCTTAACGATTGCAGCATCGGCGTAAGGAAGCGTGCCGGGTGTGATGCTTCGCACTCCGCGACGGGTTCTGGCGTACCGGAGACGGGGATTTCGCCGCCACCACTGCCGCCTTCt                                                                                                                                                                                                                                <  2:464232/249‑1 (MQ=255)                                               attatCTGGTGGATGCGTCTTATCAGCAGGCGGGGAATGTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTGCGCCGCCACCACTGCCGCCTTCTGGTCCCAGTTCGACAATCCAGTCAGCGGt                                                                                                                                                                                                   <  1:171892/249‑1 (MQ=255)                                                      ggtggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGAt                                                                                                                                                                                             <  1:90799/248‑1 (MQ=255)                                                      ggtggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCAc                                                                                                                                                                                          >  1:233372/1‑251 (MQ=255)                                                        tggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGt                                                                                                                                                                                        >  1:102200/1‑251 (MQ=255)                                                                        gtagGCGGTGAATTTACTGCCGTAAGAAATGCGTAAATTGCTGTTGCACCTATGAAAGGAAATGGCCGTCCAGAAAGCGGCCTTACCAACTACAGCATCGGCTTAAGTAAGCTTGCCGTGTGGTATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGTGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCATCGGTTTTGATCCCGTCGAGATTGTGCTAAAt                                                                                                                                                                        <  2:166712/249‑1 (MQ=255)                                                                          agGCGGTGAATTTACTGCCGGACGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGGGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGACGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATc                                                                                                                                                                       >  1:37874/1‑250 (MQ=255)                                                                              ggTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATAACGTCGAGATTGTGCTCAATcaccac                                                                                                                                                                  >  2:136282/1‑251 (MQ=255)                                                                               gTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATcaccacc                                                                                                                                                                 >  2:219028/1‑250 (MQ=255)                                                                                            cGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCt                                                                                                                                                    >  1:466388/1‑251 (MQ=255)                                                                                                   aaaaCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCGCTGCCGCCTTCTGGTCCCAGGTCGACATTCCAGTCAGCGGGTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATcgcg                                                                                                                                             <  2:46545/251‑1 (MQ=255)                                                                                                              tGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAGCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCCCCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGc                                                                                                                                   <  1:181165/250‑1 (MQ=255)                                                                                                               gCTGGTGCAACTCTGAAAGGATAAGGGCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTGTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCTCTGTTTAGGCa                                                                                                                                  <  2:266664/250‑1 (MQ=255)                                                                                                                cTGGTGCAACTCTGAACGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTTAATCACCACACTGGTGTTGCCCTGATCGCGCAGTTTATGCAGt                                                                                                                                >  1:315418/1‑251 (MQ=255)                                                                                                                  tgTGCATCTCTGAAATGAAAATGCCGCTCAGAAAGCTGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTACCGTGTGTGATGCTTCGCACGCCGCGACGTTTTCTGGCGTACCGGAGACGAGGATTGCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCAAGATGGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTa                                                                                                                               <  1:281632/249‑1 (MQ=255)                                                                                                                     gCAACACTGAAAGGAACAGGCCGGTCAGAAAGCAGCCTTAACGATTACAGCACAGGCGTAAGGAAGCGTGCCGTGTGTGATGCTGCGCCCTCCGCGACGGTTACTGGCGTCCCGAAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGTTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTc                                                                                                                           <  2:138844/251‑1 (MQ=255)                                                                                                                       aaCTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                                                                                                                          >  2:191037/1‑250 (MQ=255)                                                                                                                       aaCTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                                                                                                                          >  1:152727/1‑250 (MQ=255)                                                                                                                        aCTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTCACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                                                                                                                          >  2:80241/1‑249 (MQ=255)                                                                                                                                 ggAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTg                                                                                                               >  1:279844/1‑251 (MQ=255)                                                                                                                                 ggAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTg                                                                                                               >  1:116699/1‑251 (MQ=255)                                                                                                                                          cGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCCAGCCGTGTGTGATGCTTCGCACTCCGCGACGGCTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCAAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCCGTTTATGCAGTACGTCGAGCAGATGCTGACTATCGGc                                                                                                      >  1:289297/1‑251 (MQ=255)                                                                                                                                           gCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGCGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCg                                                                                                     >  1:315203/1‑251 (MQ=255)                                                                                                                                           gCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGAGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCg                                                                                                     >  1:448862/1‑251 (MQ=255)                                                                                                                                             tCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGa                                                                                                    >  2:228280/1‑250 (MQ=255)                                                                                                                                              cagaacacggacttcccGATTACCGCATCGGCTTAAGGAAGCCTGCCGTGTGTGATGATTCGCACTCCGCGACGGTTTCTGGCGTACAGGAGACGCGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCTGCGGTTTTGATCACGTCGAGTTTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGa                                                                                                    >  2:69209/17‑249 (MQ=255)                                                                                                                                               aGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGCCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGt                                                                                                 >  1:297429/1‑251 (MQ=255)                                                                                                                                                    gCGGCCTTAACGATTACAGCATCGGCTTAAGGACGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGa                                                                                            <  2:246824/251‑1 (MQ=255)                                                                                                                                                     cGGCCTTAACGATTACAGCTACGGCTTAAGGAAGCGTGCCGTGTGGGCTGCTTCACACTCCGCGACGGTACCTGGCGTAGCGGAGACTAGGCTTTCGTCGCCACCACTGCCGCCTTCTGGGCCCAGGTCGACAATCCAGTCGGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCCCAATAGGGTTGCCCTGTTCGCGCAGTTTATGAAGTACGTCGAGCAGTGGCTGAATATCGGCGAAGTGCAGAc                                                                                           >  2:415234/1‑251 (MQ=255)                                                                                                                                                                tttACAGCACCGGCTTAAGGTAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTATGGTCCAAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCTCCACAATGGGGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGc                                                                                >  2:64489/2‑251 (MQ=255)                                                                                                                                                                     aGCATCGGCTTAAGGAAGCGTGCCGTATGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCtcgtc                                                                           <  1:16243/251‑1 (MQ=255)                                                                                                                                                                       cATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCtcgtcgg                                                                         >  2:131228/1‑250 (MQ=255)                                                                                                                                                                             cTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAatat                                                                   >  2:249887/1‑251 (MQ=255)                                                                                                                                                                              ttAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCACTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAatat                                                                   >  1:12055/1‑250 (MQ=255)                                                                                                                                                                                aaGGACGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATAc                                                                 >  1:393994/1‑250 (MQ=255)                                                                                                                                                                                        gTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGCTCGCGCAGTTTATGCATTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGc                                                        >  1:4699/1‑251 (MQ=255)                                                                                                                                                                                        gTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGc                                                        >  1:85466/1‑251 (MQ=255)                                                                                                                                                                                               gtgtgATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGc                                                 >  1:112983/1‑251 (MQ=255)                                                                                                                                                                                                     tGCTTCACACTACGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGCCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGtt                                           >  2:31688/1‑251 (MQ=255)                                                                                                                                                                                                         tCGCCCTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCGTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTg                                        <  2:449590/250‑1 (MQ=255)                                                                                                                                                                                                         ccGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGa                                       <  1:219028/250‑1 (MQ=255)                                                                                                                                                                                                          cGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGAc                                      >  1:128825/1‑251 (MQ=255)                                                                                                                                                                                                                ccGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCa                                <  2:112983/251‑1 (MQ=255)                                                                                                                                                                                                                    gACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCAcgcg                            >  1:76767/1‑251 (MQ=255)                                                                                                                                                                                                                    gACGGTTTCTGACGTACCGGCGACGAGGATTGCGACGCCAACACCGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGAGGTTTTGATCCCGTCGAGATTGTGCTCAATCACCACCATGGTGTTGCCCTGCTAGCGCAGTTTATGCAGTACGACGAGCAGTAGCTGAATATCGGCGAAGTGGAGACCGGTGTGCGGCTCGTCGAGCATATACAGCGTCTGCCCGGTGCCACGTGTTGACAGTTGAcgcg                            >  2:394253/1‑251 (MQ=255)                                                                                                                                                                                                                       ggTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGcc                          >  2:242359/1‑250 (MQ=255)                                                                                                                                                                                                                             tGGCGAACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCCGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGGGGTCGGCTCGTCGCGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTc                    >  2:436333/1‑250 (MQ=255)                                                                                                                                                                                                                              ggCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCa                   <  2:228902/250‑1 (MQ=255)                                                                                                                                                                                                                                   aCCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCt             >  2:320595/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTTAGCGGTTGTTATCACGTCGAGATTGTGCTCAATCACCACAATGGGGTTGCCCTGATCGCGCATTTTATGCAGTACGGCGAGCAGTTGCTGAATATCGGCGTAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTGTGACAGTTCACGCGCCAGCTTCACGCGCTgg           <  2:460557/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTgg           >  1:51088/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGAAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGAGTCTGCACGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTggg          >  1:274339/1‑251 (MQ=255)                                                                                                                                                                                                                                       gagaCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGGGCAGACCGGTGGTCGGCGCGTCGAGAATATACAGCTTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGc         <  1:206325/251‑1 (MQ=255)                                                                                                                                                                                                                                         gaCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCCGCTTCACGCGCTGGGCtt       >  2:108363/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTGGACAGTTCACGCGCCAGCTTCACGCGCTGTGCTTc      <  2:275017/251‑1 (MQ=255)                                                                                                                                                                                                                                             aGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGCTTCAcc   <  1:191037/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggATTTCGCCGCCACCACTGCCGCCTTCTGGCCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGTCTTCACCg  <  2:384825/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       TGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCGAAGTGCAGACCGGTGGTCGGCTCGTCGAGAATATACAGCGTCTGCCCGGTGCCGCGTTTTGACAGTTCACGCGCCAGCTTCACGCGCTGGGCTTCACCG  >  NC_000913/4270889‑4271376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑