Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,265,668	C→T	T452I (ACC→ATC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,668	0	C	T	100.0%	136.5 / NA	40	T452I (ACC→ATC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/23);  total (17/23)

CGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACG  >  NC_000913/4265428‑4265902                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           cGAAAGAACTGAACGGGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTGCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACATCGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGt                                                                                                                                                                                                                                  <  2:357017/251‑1 (MQ=255)    aaGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCaa                                                                                                                                                                                                                               <  1:125670/251‑1 (MQ=255)       aaCTGCACGTGCCGGAGGTGGCGCTGTCCCAGTTGCAACGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGAGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATgg                                                                                                                                                                                                                            >  2:271747/1‑251 (MQ=255)                             gcTGTCCCAGTTGAACCGTTCTATGGAACTACGGGCCTACAAACGCCAGGTAAACTCCGACCTGATTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGGTTAGCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCTTCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTGCGACAACTATGCgg                                                                                                                                                                                                      <  2:262134/251‑1 (MQ=255)                                gTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCggg                                                                                                                                                                                                     <  1:2055/249‑1 (MQ=255)                                gTCCCAGTTGAACCGTGCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGAGGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGTTAAACAACGTAACGGCCCAATCGGGACGGTACGCCGGATCTTTAACGGTCAATGGGCGCGCATCGACAACTATTCGGGGc                                                                                                                                                                                                   <  2:292274/251‑1 (MQ=255)                                                     ggAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaat                                                                                                                                                                                 <  1:271747/248‑1 (MQ=255)                                                     ggAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACGATGCGGGGCCGCAGTACGACGACGaataat                                                                                                                                                                              <  1:358818/251‑1 (MQ=255)                                                      gAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataata                                                                                                                                                                             <  1:170641/251‑1 (MQ=255)                                                               gCCGACAAACGCCCGGTCAACTCCGACTTGCGTGAATCTGGCGCTATCGAGCAGGATGCGGGCTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCATTACGACGACGAATAATAATTAtttt                                                                                                                                                                     <  2:390817/250‑1 (MQ=255)                                                               gCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTGTAGCGAGCAGGATGCGGATTTTATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCAGCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCTACAACTATGCGGGGCCGCAGTACGACGACTAATATTAATTATTTTa                                                                                                                                                                    <  2:273667/251‑1 (MQ=255)                                                               gCCGACAAACGCCCGGGCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGTTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTa                                                                                                                                                                    <  1:183238/251‑1 (MQ=255)                                                                  gACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATg                                                                                                                                                                  >  1:55789/1‑250 (MQ=255)                                                                           ccGGTCGACTCCGACCTGCTTTAATCTGGCTCTATCGAGCAGGATGCGGACGTGATCATGTTTATCTATCGTGAGGAGTTGTATCACGAAAACAGGGATTTAAATGGCATCGCTGAAATTATTTTCGGTAAACAACGTATCGGCCCAATCTGTACGGTTGGCCTGATCTTTAACGTTCTATGGTGGCGCTTCGACAACTATGTGTTGGCGCAGTACTAGGTCGAATAATAATTATTTTATGAATTAGGTaa                                                                                                                                                        <  1:397201/251‑1 (MQ=255)                                                                                     cctaCCTGCGTGAATCTGTCTCTATCTAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGTACAGTACTCCTGATCTGTAACGGTCAATGGTCGCTCATCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGTTAATTAAAGCaaa                                                                                                                                              <  1:29722/248‑1 (MQ=255)                                                                                              gTGAATCAGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGCTGAGGTGTATCACGACACCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCTAAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGTCAACCATACGGGGCCGCAATACGACGACGAATAATAATTATTTTATGATTTAGGAAATTAAAGCAAACACTTATc                                                                                                                                      >  2:160036/1‑250 (MQ=255)                                                                                               tGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTc                                                                                                                                                                                                                          <  2:8855/165‑1 (MQ=255)                                                                                               tGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTc                                                                                                                                                                                                                          >  1:8855/1‑165 (MQ=255)                                                                                                                     gagtcGGACTGGATCATTTGTATCTACCGTTATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTAGCGTTAAACAACGTAACGGCCCAGTCGGGACGGTACGCATGATCTTTAACGGGCAATGGTCGCTCTTCGTCAACTATGCGTGGCCGCAGTACGACGAGGAATAATAATGATTTTATGAATTAGGTAATTTACGCAAACACTGAGTAATGAACACAAATGCAAGCGGCaa                                                                                                              <  2:465173/247‑1 (MQ=255)                                                                                                                          ggACTTGCTCATGTTTCTCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCACGCGGCAACtgtt                                                                                                         >  2:287924/1‑251 (MQ=255)                                                                                                                                    aTGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAcc                                                                                               >  1:435738/1‑251 (MQ=255)                                                                                                                                    aTGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATGTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAcc                                                                                               <  1:233014/251‑1 (MQ=255)                                                                                                                                                cGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGCCAACTATGCGGGGACGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAAAACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCACTGc                                                                                   >  1:262128/1‑251 (MQ=255)                                                                                                                                                                         gTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGa                                                                                                     >  1:73739/1‑208 (MQ=255)                                                                                                                                                                         gTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACGTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGa                                                                                                     <  2:73739/208‑1 (MQ=255)                                                                                                                                                                                   aGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCt                                                >  1:221068/1‑251 (MQ=255)                                                                                                                                                                                    ggCATCGCTGAAATTATTAGCGGGAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCTCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAATCAAACCCTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCGTCGTGAACTGGCCCCTg                                               <  1:286375/251‑1 (MQ=255)                                                                                                                                                                                     gcatcgacgaacttattaTCGGTAAACAACGTAAAGGCACAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGCCCACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCAGCAACGTCTTCGTGAACTGGCCCCTGc                                              >  2:311558/13‑251 (MQ=255)                                                                                                                                                                                         cgcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAg                                           <  1:27457/250‑1 (MQ=255)                                                                                                                                                                                                 ttattaTCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGt                                  >  2:370574/1‑251 (MQ=255)                                                                                                                                                                                                   attatCGTTAAACAACGTAACGGCCAAATCGGGACGGTACGCCTGATCTTTAACGTTCAAGGGTCTCGTTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTAGGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACAAAAATGCAAGGGGCAACTGTGGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTg                                <  1:199156/251‑1 (MQ=255)                                                                                                                                                                                                      atCGGTAAACTACGTAACGGCCCAATCGGGACGGTCCGCTGGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGCCCGCAGTCCGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCATCTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCg                              <  1:257386/250‑1 (MQ=255)                                                                                                                                                                                                      atCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCgg                             <  1:233309/251‑1 (MQ=255)                                                                                                                                                                                                          gTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGCCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCggtggt                         >  1:326083/1‑251 (MQ=255)                                                                                                                                                                                                                aacGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGc                   >  2:315249/1‑251 (MQ=255)                                                                                                                                                                                                                aacGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGc                   >  1:463261/1‑251 (MQ=255)                                                                                                                                                                                                                   gTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGCAAGCGa                 >  2:176127/1‑250 (MQ=255)                                                                                                                                                                                                                       cGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCt            >  1:246638/1‑251 (MQ=255)                                                                                                                                                                                                                          cccAATCGGGACGGTACCCCTGATGTTTAACGGTCAATGGTCGCGTTTCGACCACTACGCGGGGCCGCAGTACGACGACTAATAATAATGATTTTATGAATTAGGTAATGAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTACCCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAATTGGTTGCTGTGGTTAAAGCGATCGCTTAt         <  1:106269/251‑1 (MQ=255)                                                                                                                                                                                                                                 gggACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTGGCGGTGGTGAAAGCGAACGCTTATGGTCACg  >  2:138164/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           CGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACG  >  NC_000913/4265428‑4265902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑