Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,743	C→T	intergenic (‑256/+61)	arpA ← / ← iclR	regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,743	0	C	T	100.0%	109.7 / NA	33	intergenic (‑256/+61)	arpA/iclR	regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/14);  total (19/14)

ACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATC  >  NC_000913/4222525‑4222992                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                          aCTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGAGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGc                                                                                                                                                                                                                            >  1:244983/1‑250 (MQ=255)                    aaTTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACGTTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGc                                                                                                                                                                                                        >  1:298067/1‑251 (MQ=255)                           tatGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGAGCAGAATATTGCCTCTGCCCGCCAGaaa                                                                                                                                                                                                  >  2:377991/1‑250 (MQ=255)                                                  cATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGc                                                                                                                                                                          <  1:337142/251‑1 (MQ=255)                                                              aaTCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCActtc                                                                                                                                                              >  1:431111/1‑251 (MQ=255)                                                                          cAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTGAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAAGATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTcgccgcgtt                                                                                                                                                  <  2:221835/251‑4 (MQ=255)                                                                             ccGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACTCTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAAt                                                                                                                                               <  1:317392/251‑1 (MQ=255)                                                                                 aaaaGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCAcc                                                                                                                                           >  1:384481/1‑251 (MQ=255)                                                                                    aGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCTGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCTCCATc                                                                                                                                        <  1:68345/251‑1 (MQ=255)                                                                                     gTTTCGGTGGAATGAGATCTTGCTATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATcg                                                                                                                                       <  2:117863/251‑1 (MQ=255)                                                                                                                   ttagtaatTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGAATGACATTAAGATATTTTGGCGTCCATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGACTATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGTTCATCGGTa                                                                                                          >  2:368732/5‑250 (MQ=255)                                                                                                                        aatTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACg                                                                                                     <  2:105957/250‑1 (MQ=255)                                                                                                                                  tttGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGAATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTAACGCGGTCATCGGTAATACGTGaactcagtc                                                                                          >  1:12206/1‑244 (MQ=255)                                                                                                                                       tGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGa                                                                                      >  1:269809/1‑250 (MQ=255)                                                                                                                                            aaaaaTAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGTCATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAg                                                                                 <  1:152297/250‑1 (MQ=255)                                                                                                                                               aataatGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAAt                                                                             >  2:352935/1‑251 (MQ=255)                                                                                                                                                        gAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCaa                                                                     >  1:211020/1‑250 (MQ=255)                                                                                                                                                          gTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACATACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACg                                                                  <  1:156669/251‑1 (MQ=255)                                                                                                                                                                    gtgtgtGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCAACTCAGGTATGAAGGGCAGAATATTGCATCTGCACGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCACACGGTACACGGTg                                                        >  1:430975/1‑251 (MQ=255)                                                                                                                                                                       tgtgAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTc                                                     >  2:142434/1‑251 (MQ=255)                                                                                                                                                                        gtgAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTc                                                     <  1:212968/250‑1 (MQ=255)                                                                                                                                                                                   gACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACTCTTATTCTATTGCTACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGACACAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTAATCGGTACTACGTGAAATCGGTCCGGACCTAGAAATTGCGGCAAACGGTTCACGGAGCTCATCGCAAAtccc                                         >  2:43630/1‑248 (MQ=255)                                                                                                                                                                                             tATTTTGGCGTCAATGCGATTAACAGACACTCGTATTCTATTGCCACTCAGGTAGGAGGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACACGGTCACGCGGGCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAg                                <  1:43630/250‑1 (MQ=255)                                                                                                                                                                                                   ggCGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGt                         >  2:373690/1‑251 (MQ=255)                                                                                                                                                                                                     cGTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGt                         >  1:444005/1‑249 (MQ=255)                                                                                                                                                                                                      gTCAATGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGc                      >  1:243361/1‑251 (MQ=255)                                                                                                                                                                                                         aaTGCGATTAACAGACTCTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATGGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGcccc                   <  2:444005/251‑1 (MQ=255)                                                                                                                                                                                                          aTGCGATTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCa                  <  1:377991/251‑1 (MQ=255)                                                                                                                                                                                                               aTTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTa                        <  1:301565/240‑1 (MQ=255)                                                                                                                                                                                                               aTTAACAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTa                        >  2:301565/1‑240 (MQ=255)                                                                                                                                                                                                                  aaCAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGt          >  1:359236/1‑251 (MQ=255)                                                                                                                                                                                                                    cAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCg                                                                                        >  1:392778/1‑171 (MQ=255)                                                                                                                                                                                                                    cAGACACTCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCAGCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCg                                                                                        <  2:392778/171‑1 (MQ=255)                                                                                                                                                                                                                          cccTTATTCTATTGCCTCTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCTTACCCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACGCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGTCAACGTAGCCCCAGTGCATTTTCCTCATc  <  2:386393/251‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                          ACTATCAGAACAGTGAAAAAATTTAATATGATTACAACTAAAGAAATATCATAAATCGCTCAATCTCATAATGCAGCCGTAAAAGTTTCGGTGGAATGAGATCTTGCGATTTTGTTAATAATTAAGTGGTTTGATGTTTAAAAATAATGCTGAGTTAATAAAAGTGTGTGAAGTGTATGACATTAAGTTATTTTGGCGTCAATGCGATTAACAGACACCCTTATTCTATTGCCACTCAGGTATGATGGGCAGAATATTGCCTCTGCCCGCCAGAAAAAGTCAGCGCATTCCACCGTACGCCAGCGTCACTTCCTTCGCCGCTTTAATCACCATCGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGTGCATGTTCCTCATC  >  NC_000913/4222525‑4222992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑