Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 502,232 G→A T336I (ACC→ATC)  ybaL ← putative transporter YbaL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913502,2320GA96.2% 171.9 / ‑5.5 53T336I (ACC→ATC) ybaLputative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/27);  minor base C (2/0);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAC  >  NC_000913/501991‑502477
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
aaTATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCg                                                                                                                                                                                                                                              >  1:148360/1‑251 (MQ=255)
    tccctttggttcgttttctcttctGCGATGGCCTCTTCAAGCGTCTGCTCCTCCAGCTTTCCG‑TTCTTGGCCAGATATTTCTCCAGTAGTGCGAACATTCCCGGTTTG‑GGCATAATCGACAGGATCGCCCCTGCCAGTACCAGTTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTAc                                                                                                                                                                                                                                           <  2:339171/235‑1 (MQ=255)
     ccACTGGGATCTGCTCCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGCACGt                                                                                                                                                                                                                                         >  2:61588/1‑251 (MQ=255)
         ggggATCTGCTTCTCTTCTCCGATGGCCTCTTCCAGCGTCTGCTCTCCAAGGGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTCCCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTGGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTggg                                                                                                                                                                                                                                     <  2:188291/250‑1 (MQ=255)
          ggtatcttcttctctttttcctgtGCCTCTCTCAGCGTCTGCTCTTCC‑GTCTGTCGTGTCTTCGCCATATATTTCTCCAGTAGTGCTAACATTCCCGGGTTG‑AGCATAATCGACAGTATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAACTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCTATGATTAATGCCGTACGTTgg                                                                                                                                                                                                                                      <  2:247329/226‑1 (MQ=255)
                       cttcttCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGt                                                                                                                                                                                                                       >  1:341573/1‑251 (MQ=255)
                                ttGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTCGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGCTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGCCGCACCAgg                                                                                                                                                                                                              >  2:179289/1‑251 (MQ=255)
                                 tGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATCCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAgg                                                                                                                                                                                                              >  1:265834/1‑250 (MQ=255)
                                         ccAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGc                                                                                                                                                                                                      <  1:136979/250‑1 (MQ=255)
                                         ccAGCGTCTGCTCTCCCAGCGTTCCG‑GTCTTCCCCAGATATTTCCCCAGTAGTGCGAACAGTCCCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCg                                                                                                                                                                                                     <  1:166333/251‑1 (MQ=255)
                                                     ctTCCAGCGTTTCG‑GTCTTCGCCAGATTTTTCCCCAGTAGTGCGAACAGTCCCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGaat                                                                                                                                                                                           <  2:2761/249‑3 (MQ=255)
                                                       tCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACtt                                                                                                                                                                                          <  1:121012/248‑1 (MQ=255)
                                                       tCCAGCGTTTCG‑GTCTTCGCCAGATATTCCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGACCGCCCCGGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTa                                                                                                                                                                                         <  2:341573/249‑1 (MQ=255)
                                                        ccggtgttccG‑GTCTCCGCCAGAGATTTCTCCAGTAGTGCGAACATTACCGGGTTG‑AGCAAAATCGACAGGATCCCCCCTGCCGGTACCAGGTTTTGGCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCGGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCCCCAGGAAAAATGCGGCTAACGTCTTAcc                                                                                                                                                                                       <  2:348380/241‑1 (MQ=255)
                                                         cAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGATTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCaa                                                                                                                                                                                     <  1:399764/251‑1 (MQ=255)
                                                                 cG‑GTCTTCTCCAGAAATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAACCGACAGGAGCGCCCCTGCCAGTACCGGGTTGTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCTCCAAGCTGCGCCAGGCTGGCGGCGATGATTAATGCCTTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaata                                                                                                                                                                             <  1:213899/251‑1 (MQ=255)
                                                                             aGATATTTCTCCAGTAGTGCGACCATTACCGGGTTG‑ATCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGGAAATTCAATGCCATTCCCAGGCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGt                                                                                                                                                                  <  2:360896/251‑1 (MQ=255)
                                                                                   ttcgccagtgtggCGAATAGTAC‑GGGTTGGAGCAAAACCGCCAGGACCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCGGCGGCAGTAAATCCAATGCCATTCCCAGGCCCGCCAGTATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTTGTAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCg                                                                                                                                                                <  2:71388/235‑1 (MQ=255)
                                                                                   ttCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATAGACATTATCGCACCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATACAATGCCATGCCCAGCCCCGCCAGGATAAACGCGAACTCACAAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTAAGTTGTGAGTGACCAAACAGACGCACGAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCgac                                                                                                                                                              >  2:307090/1‑247 (MQ=255)
                                                                                    tctcCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGc                                                                                                                                                           >  1:381673/1‑251 (MQ=255)
                                                                                             gtggaaaCAGTACCGGGTGG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTGTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTGCGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGc                                                                                                                                                  <  1:12421/246‑1 (MQ=255)
                                                                                             gtGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGc                                                                                                                                                  <  1:366662/251‑1 (MQ=255)
                                                                                               gCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCCCTGCCAGCgg                                                                                                                                                >  1:172620/1‑251 (MQ=255)
                                                                                                 gAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGGCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGTCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAAAAATCGCCAGCGTCGCCAGCACGGCCCGCGGtt                                                                                                                                              >  2:351144/1‑251 (MQ=255)
                                                                                                  aaCAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTg                                                                                                                                             >  1:11142/1‑251 (MQ=255)
                                                                                                                             cAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCacac                                                                                                                    >  1:169823/1‑247 (MQ=255)
                                                                                                                                 aTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCTCCAGCACTGCCAGCGGTTGCTGCATCAGAATTAACGGATCAAACAACAt                                                                                                               >  1:88739/1‑251 (MQ=255)
                                                                                                                                             aGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGAc                                                                                                   >  2:176972/1‑251 (MQ=255)
                                                                                                                                                      ttttGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCaa                                                                                                                      >  2:393168/1‑223 (MQ=255)
                                                                                                                                                      ttttGCCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCaa                                                                                                                      <  1:393168/223‑1 (MQ=255)
                                                                                                                                                                    cGGCAGTAAATCCAATGCCCTTACCAGCCCCGCCAGGATAAACGCGAACGCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACGTACCAAACAGAATAATCGCCAGCGACGCCAGCACTGCCAGCGGTGGCTGAATCAGAATTAACGGATCAAACAACATCCCGTCGGAGACAAAAAACAGCACCGCAAAcgcga                                                                            <  2:62953/251‑2 (MQ=255)
                                                                                                                                                                     ggCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTc                                                                           <  1:214255/251‑1 (MQ=255)
                                                                                                                                                                       aatgaaaTTCATTCCCATCCCCAGTCCCGCCAGGATAAACGCGAACGCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTTAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAACCGCGTcgc                                                                         <  2:159679/247‑1 (MQ=255)
                                                                                                                                                                          tAAATTCAATGCCATTCACAGTCCCGCCAGGCTCAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGCAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCACCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACCTCCCGAAGGAGACAAAAAACAGCACCGCAAAcgcaacgcgc                                                                       >  2:271172/1‑243 (MQ=255)
                                                                                                                                                                           aaaTTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGACGCGCa                                                                      >  1:232120/1‑250 (MQ=255)
                                                                                                                                                                                cAATGCCATCCCCGGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCa                                                                <  2:361103/251‑1 (MQ=255)
                                                                                                                                                                                   tGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGCGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCg                                                             >  1:409102/1‑251 (MQ=255)
                                                                                                                                                                                     ccATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTa                                                           <  1:351144/251‑1 (MQ=255)
                                                                                                                                                                                          cccAGTACCGCCACGATAAACGAGAACTCACCAATATGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCCCCAGGAAAAATGCGGCTAACGCCTTACCCAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAAAATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg                                                      >  2:189716/1‑251 (MQ=255)
                                                                                                                                                                                          cccAGGCCCCCCAGGAAAAACGCGAACTCCCCAATCAGCGCCAGGATGGCGGCGATGCTTACTGCCGTAACTTGGGAGTGACCAAACAGACGCCCCCGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTCGCTGACTCAGAATTAACGCATCAAACAACATCCCGACCAAGACAACAAACAGCACCGCAAACCCGTCGCGCAATGGCaccgcacagtg                                                      >  2:445775/1‑241 (MQ=255)
                                                                                                                                                                                             aGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaata                                                                                                                                                                             <  1:210754/129‑1 (MQ=255)
                                                                                                                                                                                             aGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaata                                                                                                                                                                             >  2:210754/1‑129 (MQ=255)
                                                                                                                                                                                             aGTCCCGACAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggc                                                   >  1:128626/1‑251 (MQ=255)
                                                                                                                                                                                                  cGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCAc                                              <  2:148360/251‑1 (MQ=255)
                                                                                                                                                                                                   gCCAGGATACACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAACCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAAGCGTCCCGCAATGGCAGCGAAACGGGGGAGGCACg                                             >  2:272186/1‑251 (MQ=255)
                                                                                                                                                                                                      aGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTg                                          >  2:388504/1‑251 (MQ=255)
                                                                                                                                                                                                           aaaCGCGAACTCACCAATCGGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCa                                     <  1:176972/251‑1 (MQ=255)
                                                                                                                                                                                                              cgcgAACTCACCAATCTGCGCCAGGCTGGCGGCGATGCTTAATGCAGTACGTTGGGCGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGACTAATCGCCAGCGTCGCCAGCACTGCCATCGGTTGCTGAATCAGAATTAACGGATCAAACACCATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGAGGCACGGTGACTCAGtt                                  >  1:262388/1‑251 (MQ=255)
                                                                                                                                                                                                              cgcgAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaa                                                                                                                                                                               <  1:21102/110‑1 (MQ=255)
                                                                                                                                                                                                              cgcgAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaa                                                                                                                                                                               >  2:21102/1‑110 (MQ=255)
                                                                                                                                                                                                                               gccccAGGCTGGCGGCGATGATTAAGGCCGTACGTTGGGAGTGACCAAACAGTCGCTCCAGGAAAAATGCGGCTAACGACGTCCCAAACAGAATAATCGCCAGAGTCGCCAGCACTGCCATCGGTTGCTGAATCAGAATTAACGGATCAAACATCATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCTTATCGTGGGCGGCACGGTGACTCAGTCCAGACTCGTTCAGt                    <  2:184196/245‑1 (MQ=255)
                                                                                                                                                                                                                                     ggCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCAAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGTCTCGTTCAGTACCATcacg           >  1:429425/1‑248 (MQ=255)
                                                                                                                                                                                                                                              cgATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAc  <  1:94031/251‑1 (MQ=255)
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AATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCG‑GTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTG‑AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAC  >  NC_000913/501991‑502477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑