Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,827	C→T	noncoding (74/76 nt)	thrT →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,827	0	C	T	100.0%	171.5 / NA	50	noncoding (74/76 nt)	thrT	tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/27);  total (23/27)

CCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGCT  >  NC_000913/4175581‑4176072                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ccGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGGTGAGGATGCGGGTGCGATTTCCGCTGCCCGCTCCAAGAGGTGGTGATATAGCTCAGTTGGTAGAGCGCACCCTTTGTAAGGGTGAGTTCGGCAGTTCGAATCTGCCTATCAGCATCAct                                                                                                                                                                                                                                                   <  2:234529/251‑1 (MQ=255)    agaCGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCActtct                                                                                                                                                                                                                                                >  1:82067/1‑251 (MQ=255)     gaCGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCActtctt                                                                                                                                                                                                                                               <  1:451900/251‑1 (MQ=255)     gaCGATAAGTTCGCTTACCGGCTAGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCActtctt                                                                                                                                                                                                                                               <  2:123673/251‑1 (MQ=255)                cGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCt                                                                                                                                                                                                                                    >  1:107407/1‑251 (MQ=255)                    tACCGGCTCGAATCAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGGAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTAACTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGGGCTGATATAGCTCAGGTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCTAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGttt                                                                                                                                                                                                                                >  2:160866/1‑251 (MQ=255)                         gcccGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTcc                                                                                                                                                                                                                           <  2:168257/248‑1 (MQ=255)                                aaaaGAGAGCTTCTCTCGAGATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCGATTACCTCATCCTTCCAAGCTGATGATGCGGGTTCGATGCCCGCTGCCCGGTCCAGGATGTGCTGATATAGCTCAGTTGGTAGAGCGCTCCCTTGGTAAGGTTGAGGTCGTCAGTTCGTATCTGCCTAGCAGCTTCACTTCTTTTCGCCTCGCCGTTTTGTCCTTCTGtt                                                                                                                                                                                                                    <  2:297471/250‑1 (MQ=255)                                 aaaGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGtt                                                                                                                                                                                                                    <  1:205246/250‑1 (MQ=255)                                      gagCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGc                                                                                                                                                                                                              <  1:58384/251‑1 (MQ=255)                                      gagCTTCTCTCGATAGTCAGTGCAGAATGAAAATCAGGTTGTCGAGTTCTAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGTTGCCTGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTTTCAGCATCACTTCTTTTCTCCTCCCTGTGTTTTCCTTCTGTTTATTGc                                                                                                                                                                                                              <  1:394555/251‑1 (MQ=255)                                            ctctcGATATTCAGTGAAGAATGAAAATCAGGTAGCCGAGTTCAAGGATGCGGGCGTCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGAGGCGGGATCAATTCCCGCTGCCCGCTCCAAAATATGCTGATATAGCTCAGTTGGTAGCGCGCACCCTTGTGAAGGGAGAGGTCGGCAATTCGAATCTGCCTATCAGCATCACTTATTTTCTCCTCCCTGTTTTTTCCTTATGTTTATTGCATTct                                                                                                                                                                                                         >  2:350590/1‑249 (MQ=255)                                                               aataaaactCAGGTAGCCTAGCTCCAGGATGCGGTCATCATCCAATGACTCTTACCTCCGCATACAAAGCTGGTGATGCGGGTTCGATTACAGCTCGACGCGCCAGGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCATTGGAAAGATGGCGGTCGGCAGTTCGACTCTGCCTATCAGAATCAATTCTTTTCTCCTCCCTGTTTTGTCCTTCTGTTTATTGCATTCAACACGTCGGGCATGTTGCCt                                                                                                                                                                                     >  2:199133/9‑251 (MQ=255)                                                                                               gggCATCGGATAAAGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACACTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCgt                                                                                                                                                     >  1:91036/1‑251 (MQ=255)                                                                                                     cGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCtt                                                                                                                                                <  2:155509/250‑1 (MQ=255)                                                                                                      gTATAATGGCTATTACCTCAGCCATCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATGGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTag                                                                                                                                              <  2:59488/251‑1 (MQ=255)                                                                                                        ataATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGTTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTagag                                                                                                                                            <  1:312975/251‑1 (MQ=255)                                                                                                        ataATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTaga                                                                                                                                             <  1:41960/250‑1 (MQ=255)                                                                                                          aaatgCTATGACCTTAGCCTTTCAAGCTGATGATGCTGGTTCGATTCCCGCTGCCCGCTCCAAGAGGTGCTGATATAGTTCAGTTGGTAGAGCGCACCCGTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCATCGATTTATCCGTGTCTTTGAggg                                                                                                                                          <  2:136255/247‑1 (MQ=255)                                                                                                             ggCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACa                                                                                                                                       <  1:169436/251‑1 (MQ=255)                                                                                                              gCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACaa                                                                                                                                      <  2:114026/251‑1 (MQ=255)                                                                                                                   tACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGa                                                                                                                                  <  1:202483/250‑1 (MQ=255)                                                                                                                          gCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCCGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCCGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTaa                                                                                                                           >  2:302757/1‑250 (MQ=255)                                                                                                                           ccTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTc                                                                                                                                                  >  1:207384/1‑226 (MQ=255)                                                                                                                           ccTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGGGGTGATATCACCGATTTATCCGTGTc                                                                                                                                                  <  2:207384/226‑1 (MQ=255)                                                                                                                              tCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCACCAAGATGTGCTGATATAGATCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGaa                                                                                                                       <  1:260916/250‑1 (MQ=255)                                                                                                                                          atgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGt                                                                                                           >  1:360301/1‑250 (MQ=255)                                                                                                                                                       tCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGt                                                                                             >  1:162394/1‑251 (MQ=255)                                                                                                                                                                ccGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCATCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTTGTGTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCGGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCgg                                                                                    <  1:243203/251‑1 (MQ=255)                                                                                                                                                                        aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCg                                                                            <  1:107773/251‑1 (MQ=255)                                                                                                                                                                        aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCg                                                                            >  1:15646/1‑251 (MQ=255)                                                                                                                                                                          aTGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGc                                                                          >  1:147586/1‑251 (MQ=255)                                                                                                                                                                             tgctgcTATCGCTGAGTTGGAAGACCGGACCCTTGGCAAGGGTGGGGTCGGCAGTGCGAATCTGACTATCAGCCTCACTTCTGTTCTTCTCCATGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGAATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAGCGTACAAAACCGCACGTTAAAGTCGGTACTATCGGCCAc                                                                       >  2:20751/1‑251 (MQ=255)                                                                                                                                                                                 gATATAGCTCAGTTGGTAGAGCGCACCCTTGTTAAGGGTGAGGTCGGCATTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTGTTTCCTGCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCTTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACTGGAACGTCGTTACTATCGGTCACGt                                                                     <  2:21599/249‑1 (MQ=255)                                                                                                                                                                                    ataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGAcc                                                                >  1:252096/1‑251 (MQ=255)                                                                                                                                                                                          cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGt                                                           >  2:128752/1‑250 (MQ=255)                                                                                                                                                                                                gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAaacaa                                                    <  2:147586/251‑1 (MQ=255)                                                                                                                                                                                                     gcgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACGATGGTAAAACAACGCTg                                               <  1:153351/251‑1 (MQ=255)                                                                                                                                                                                                        cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTa                                                                                                                                               >  2:318286/1‑152 (MQ=255)                                                                                                                                                                                                        cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTa                                                                                                                                               <  1:318286/152‑1 (MQ=255)                                                                                                                                                                                                             ttGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCCGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGc                                       >  1:180290/1‑251 (MQ=255)                                                                                                                                                                                                                  aaGGGTGAGGGCGGCAGTTCGAATCTGCCTATCAGCATCCCTTATTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAACCCGCACGTTACCGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCCCTGCAATc                                   >  2:23667/1‑250 (MQ=255)                                                                                                                                                                                                                   aaggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCAc                                 <  1:62226/249‑1 (MQ=255)                                                                                                                                                                                                                    gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACt                                <  1:128752/251‑1 (MQ=255)                                                                                                                                                                                                                          ggTCGGCAGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGt                           >  1:337393/1‑250 (MQ=255)                                                                                                                                                                                                                                 aGTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCt                    >  2:144396/1‑250 (MQ=255)                                                                                                                                                                                                                                  gTTCGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGAAAAACAACGCTGACAGCTGCAATCACTACCGTACTGGCTa                   >  1:353579/1‑250 (MQ=255)                                                                                                                                                                                                                                     cGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAAc               >  1:27967/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGAATCTGCCTATCAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAAc               >  1:292614/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cAGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGtgct  <  2:338905/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCCGAGTTCCAGGATGCGGGCATCGTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGCT  >  NC_000913/4175581‑4176072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑