Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,139,298	G→A	A137A (GCC→GCT)	fsaB ←	fructose‑6‑phosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,139,298	0	G	A	100.0%	145.1 / NA	46	A137A (GCC→GCT)	fsaB	fructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

GAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAG  >  NC_000913/4139054‑4139543                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gAGTAGTGCCAAATGCGTCATCCCAGTCGGGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGTTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACATTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCTGTCTCCGCCCTGAGCATc                                                                                                                                                                                                                                                  <  1:299001/250‑1 (MQ=255)                         aGTCGTGTTCGAACTTCTCGATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCCGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACg                                                                                                                                                                                                                         >  1:185040/1‑251 (MQ=255)                               gtTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGTAGCAACGTAttt                                                                                                                                                                                                                    <  2:101756/250‑1 (MQ=255)                               gtTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACACCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTAtttt                                                                                                                                                                                                                   <  2:122297/251‑1 (MQ=255)                                     aCTTCTCTATAGCTGACTCTAACGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTAAGCGGTTAACATACGGAGCAACGTATTTTGCCCCt                                                                                                                                                                                                             >  2:91136/1‑251 (MQ=255)                                         ctctATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGcc                                                                                                                                                                                                          >  1:266029/1‑250 (MQ=255)                                           ctATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACAGCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCa                                                                                                                                                                                                         <  1:265738/249‑1 (MQ=255)                                                  tGACTCTACCGCAGGGGGGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCt                                                                                                                                                                                                 >  1:279406/1‑250 (MQ=255)                                                          ccGCAGGGGTGTTGAGCATTTGTTGCGCGACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAAcccg                                                                                                                                                                                        <  2:18620/251‑2 (MQ=255)                                                             cAGGGGTGTAGAGCATTTGTTGCGCTACATCTAAGAGCAGGGTGATGGATTCACATCCTGCCAGTAACCAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGCACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGCCTAATAACCCTtgt                                                                                                                                                                                     >  2:279147/1‑251 (MQ=255)                                                                  gtgtTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTgcggc                                                                                                                                                                                <  1:41242/251‑1 (MQ=255)                                                                    gtTGATCATTTGTTGCGCTACATCTAAGGGCAGGGTGCTGGATTCACATCCTGACAGTAAACAGTCCAGCGCCTGAAGCGGCGATTTAAAGCTGGATGCCAGCACCATGCTTTCTGGCGCGTGAATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGATAACATACGGAGCAACGTATTTTACCCCTGCCAGAGCGGCTAATAACCCTTGTGCGtcgc                                                                                                                                                                              >  2:122457/1‑251 (MQ=255)                                                                           aTTTGTGGCGCTACATCTAAGGGTGGGGTGATGGATTCACATCCGGCCAGTAAACAGTCCAGCGCCGGACGCGGCGTTTTAAAGCTGGCTGCCAGCGCCATGCTTTCGGGCGCGTGCATTTCTAACAGCGTTTGCAGCTGCTGAACCGTACGAATGGCGGCGCCGCCCGGAGTTTCTACTCTGTTAACATACGGAGCAACGTATGTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAc                                                                                                                                                                       <  1:194592/251‑1 (MQ=255)                                                                                    gcTACATCGAAGGGCAGGATGATGGATTCAAAACCTGCCAGTAACCAGTCCAGCGCCTGAAGCGGCGTTTTAAAGCTGACCGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTTAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACACTTGTGCGGCGCTATATACAGCAGTGcc                                                                                                                                                              >  2:322820/1‑251 (MQ=255)                                                                                       aCATCTAAGGGCAGGGTGATGGAGTCACATCCTGCCAGTAAACAGGCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCTGTCTCCGCCCTGAGCATCt                                                                                                                                                                                                                                                 <  1:308522/165‑1 (MQ=255)                                                                                        cATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgt                                                                                                                                                          >  1:135009/1‑251 (MQ=255)                                                                                            tAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtgt                                                                                                                                                        >  1:31016/1‑249 (MQ=255)                                                                                                ggCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagtact                                                                                                                                                  >  1:342882/1‑249 (MQ=255)                                                                                                  cAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCATGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCCCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTTCCAAGTGGAGTAATAc                                                                                                                                                >  2:89498/1‑251 (MQ=255)                                                                                                           ggATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCtttt                                                                                                                                        <  1:53420/250‑1 (MQ=255)                                                                                                             aTTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTGAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGGGCCAAGTGTAGTAATACCCTCTTTTTTc                                                                                                                                     <  2:226816/251‑1 (MQ=255)                                                                                                                     ccTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTAtttt                                                                                                                             >  1:150830/1‑251 (MQ=255)                                                                                                                        gCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTaa                                                                                                                           <  1:105270/250‑1 (MQ=255)                                                                                                                        gCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTa                                                                                                                            <  1:245315/249‑1 (MQ=255)                                                                                                                           aGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtgc                                                                                                                       <  1:49612/251‑1 (MQ=255)                                                                                                                                     ccAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTTCCAGAcca                                                                                                             <  2:266029/251‑2 (MQ=255)                                                                                                                                        gcgcCTGACGCGGCGGTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCTTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCtgcg                                                                                                          <  2:342557/251‑4 (MQ=255)                                                                                                                                         cgcCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCgg                                                                                                         >  1:174333/1‑251 (MQ=255)                                                                                                                                          gcCTGCCGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCTCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTGAATTGCTGCCAGATCTTCGGa                                                                                                        <  2:279406/251‑1 (MQ=255)                                                                                                                                           ccTGACGCGGCGGTTTAAAGCTGGCTGCCAGCGCCAGGCTTTCTGGCGCGGGCATTTCTAACAGCGTGTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGTTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGGGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAAGTGCTGCCAGACCTTCGGaa                                                                                                       <  2:159878/251‑1 (MQ=255)                                                                                                                                                                tggctgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAGCGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCCCCGGGATTTTcaccac                                                                                   >  2:272681/1‑250 (MQ=255)                                                                                                                                                                 ggctgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACcaca                                                                                  <  1:272681/250‑1 (MQ=255)                                                                                                                                                                                       ggCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTcgcg                                                              <  1:100883/248‑1 (MQ=255)                                                                                                                                                                                         cgcgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCTTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATCCCCGGAATAGCGTCGCGCAGgc                                                         <  2:400713/251‑1 (MQ=255)                                                                                                                                                                                         cgcgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAg                                                                                                                                   >  2:87795/1‑177 (MQ=255)                                                                                                                                                                                         cgcgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAg                                                                                                                                   <  1:87795/177‑1 (MQ=255)                                                                                                                                                                                             tGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGCGCAACGAATTTTGCCCCTGCCAGTGCGGCTACTAACCCTTGTGCGGCGCTATATACAGCTGTGCCAAGTGAAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGCATAGCGTCGCGCAGGcgctt                                                     >  1:291735/1‑251 (MQ=255)                                                                                                                                                                                                 tttCTAACAGCGTTTGAAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgcttcgct                                                 >  1:96097/1‑251 (MQ=255)                                                                                                                                                                                                               tGCAGTTCGTGAACCGTACGAATGCCGAATACGCCCTGAGCAAGTACGCGGTTAACATAAGGAGCAACGAATATTGCCACTGCCAGCGCGGCTAATAACCCTTGTGCGGCGCTATATAACGCGGTGCCAAGTGTAGTAACAAGCTCTTTTATCAGTATTTTAATTGGTGCCAGACATTCGGAAGTGACCGGAATTTTCACCACAATACCAGGAATAGCGTCGAGCAGGCGCGTCGCTTATGCCACCATccc                                   >  2:202325/1‑251 (MQ=255)                                                                                                                                                                                                                   gCTCCTGAACCGAACGAATGCCGGCTCAGCACTGAGCATCAAAGCGGTTAAAATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACAATTGAGCGGCGCTATATACAGCAGGGACAAGGGTAGTAATACACTCTTTTTTAAGTATTTTAATTGCTGCCAGAACTTCGGAAGTAACAGGGATTATCACCACAATACCCGGAAAAGAGACGCGAAGgcgcagcgcgtcgtcaaccataccctgg                               >  2:254688/1‑227 (MQ=255)                                                                                                                                                                                                                         gacccGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCGTCCACCATCCCCTGCGCGTc                          <  2:150830/247‑1 (MQ=255)                                                                                                                                                                                                                              gTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCt                    >  1:74444/1‑251 (MQ=255)                                                                                                                                                                                                                                aCGAATGCCGTCTCCGCCCTGAGCATCGACGCGGGTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATAACCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCAACGGGATTTTCACCACAATACCCGGAATAGCGACGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGAGTCGCGGCTc                   >  2:75242/1‑250 (MQ=255)                                                                                                                                                                                                                                           ctcCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACACTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGc        >  1:451631/1‑250 (MQ=255)                                                                                                                                                                                                                                                cccTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTTCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTGTCACCTCAATACCCGGAATAGCGTCGCGCAGGGTCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAg  <  2:48621/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccTGAGCATCTACGCGGATAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGCGCAAAc    >  2:181011/1‑248 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAG  >  NC_000913/4139054‑4139543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑