Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,932	G→T	F297L (TTC→TTA)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,932	0	G	T	100.0%	243.6 / NA	69	F297L (TTC→TTA)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/38);  total (31/38)

TCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACC  >  NC_000913/4084693‑4085172                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 tCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCAt                                                                                                                                                                                                                                        <  1:429303/250‑1 (MQ=255)  cAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTg                                                                                                                                                                                                                                      <  2:16874/251‑1 (MQ=255)       acaTCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTcag                                                                                                                                                                                                                                 <  1:240348/251‑1 (MQ=255)        cagcTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGAGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGGGTATTCGCGGTTTAATTTTTCATTGTTccg                                                                                                                                                                                                                                 >  2:398238/4‑248 (MQ=255)         aTCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGAGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTccgc                                                                                                                                                                                                                                >  2:301452/1‑247 (MQ=255)             ggCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGGTTAATTTTTCATTGTTCAGCAGGt                                                                                                                                                                                                                            <  2:391333/250‑1 (MQ=255)                  gtAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGTGTTGGTATAGGCTTCGGTGTATGCGCTGTTTAATTTTTCATTGTTCAGCAGGTACAGCa                                                                                                                                                                                                                      <  2:453702/251‑1 (MQ=255)                  gtAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCa                                                                                                                                                                                                                      <  1:193190/251‑1 (MQ=255)                    aGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAAt                                                                                                                                                                                                                    <  2:138053/251‑1 (MQ=255)                    aGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGGATAGGCTTCGGTGTATTCGCGGTTTACTTTTTCATTGTTCAGCAGGTACAGCAAt                                                                                                                                                                                                                    >  1:411607/1‑251 (MQ=255)                    aGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGCGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATACGTCCAGGAGGATTTCTCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTACTTTTTCATTGTTCAGCAGGTACAGCAAt                                                                                                                                                                                                                    >  2:448563/1‑251 (MQ=255)                         gAAACGTGCTGTTTCAGCAAGTGCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCAAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGcc                                                                                                                                                                                                               <  1:313947/251‑1 (MQ=255)                           agcGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTg                                                                                                                                                                                                             <  1:127404/249‑1 (MQ=255)                              gTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACa                                                                                                                                                                                                          <  1:36654/251‑1 (MQ=255)                                     ttCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGc                                                                                                                                                                                                   >  1:62356/1‑251 (MQ=255)                                                 cccacGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTAcc                                                                                                                                                                                       <  2:82320/249‑1 (MQ=255)                                                         gcgcGGATGTTGCAGCGCGGGATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGGCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCTTCGTAGCCGGTGAACAGGCCAGCTTCAAAGCCGTAATCCTCACGCACGAGCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGaa                                                                                                                                                                               <  1:448563/251‑1 (MQ=255)                                                                ggTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTGCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTTCTGGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTGTCTTTGTTCAGCATGTACAGCAATACGCCGTATATGAAAGCAATGTCAGGTCCGGAACGAATAGGGGc                                                                                                                                                                        <  2:434928/250‑1 (MQ=255)                                                                   tGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAt                                                                                                                                                                      <  1:64662/250‑1 (MQ=255)                                                                          gggTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTTGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCa                                                                                                                                                              <  1:465248/250‑1 (MQ=255)                                                                            gTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGc                                                                                                                                                            >  1:447025/1‑251 (MQ=255)                                                                            gTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGc                                                                                                                                                            >  1:378038/1‑251 (MQ=255)                                                                                       ggCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgccgcc                                                                                                                                                  <  1:146651/250‑1 (MQ=255)                                                                                           aaGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcg                                                                                                                                             <  1:48331/251‑1 (MQ=255)                                                                                                 ttttCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTaa                                                                                                                                        >  2:140239/1‑250 (MQ=255)                                                                                                        ccAGTTCATAATCCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAgg                                                                                                                                 <  1:301452/250‑1 (MQ=255)                                                                                                        ccAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAgg                                                                                                                                 <  1:140239/250‑1 (MQ=255)                                                                                                          ggTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatc                                                                                                                              <  2:115818/250‑1 (MQ=255)                                                                                                          aGTTCATAAGGCCAGGAGGATTTATCGTACTTGCGTTTTCCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCTCGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATCCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatc                                                                                                                              <  2:380502/251‑1 (MQ=255)                                                                                                             tCATAAGCCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTACTACTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTCATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGCATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatcgat                                                                                                                           >  2:270946/1‑251 (MQ=255)                                                                                                              cATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGGCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCGCACGCACGATCAGGCGGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatcgat                                                                                                                           <  2:49057/250‑1 (MQ=255)                                                                                                                tAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATcac                                                                                                                        >  1:30436/1‑251 (MQ=255)                                                                                                                     ccAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGa                                                                                                                                   >  1:55223/1‑235 (MQ=255)                                                                                                                     ccAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGa                                                                                                                                   <  2:55223/235‑1 (MQ=255)                                                                                                                     ccAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGGACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGTCGTACGCGTAAAGCGAGGATCGATCACAATc                                                                                                                    <  1:403440/250‑1 (MQ=255)                                                                                                                      cAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATc                                                                                                                    >  1:9254/1‑249 (MQ=255)                                                                                                                           ggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAAtt                                                                                                                                                                                                                                              >  1:319364/1‑122 (MQ=255)                                                                                                                              tttATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCTCGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTCCCGCCGCCGTAc                                                                                                                                              <  2:164922/215‑1 (MQ=255)                                                                                                                              tttATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAc                                                                                                                                              >  1:164922/1‑215 (MQ=255)                                                                                                                                  gcGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGACAAGGCCATCTTCAAAGCCGTAATCCTCCCGCACGATCAGGCTGGCGTTGTTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGtt                                                                                                      <  1:119939/250‑1 (MQ=255)                                                                                                                                    gTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCTTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTtgt                                                                                                    <  2:133586/251‑1 (MQ=255)                                                                                                                                      aCTTGCGTTTTTCCGCGTAGTAGCCGGTGACCAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCCGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGCTCACAATCAGCTTCGCGCCGTtgtg                                                                                                   >  2:91592/1‑250 (MQ=255)                                                                                                                                       cTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGa                                                                                                  >  1:180239/1‑250 (MQ=255)                                                                                                                                                  ccGCGTCGTAGCCGGTGACCAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTCCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAAGGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCTTTGTGAATTTTGGCTTc                                                                                       <  2:30436/250‑1 (MQ=255)                                                                                                                                                  ccGCGGCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAAGCCTCACGCACGATTAGGCGGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAAGAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGACGTTGTGAATTTTGGCTTcc                                                                                      <  2:650/251‑1 (MQ=255)                                                                                                                                                     cgtcgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATc                                                                                   >  1:326201/1‑251 (MQ=255)                                                                                                                                                      gtcgtAGCCGGTGAACAGGCCATCTTCAATGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCg                                                                                  >  1:141446/1‑251 (MQ=255)                                                                                                                                                        cgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATAGcc                                                                                >  2:455741/1‑251 (MQ=255)                                                                                                                                                         gtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTTCCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGcca                                                                               <  2:333909/251‑2 (MQ=255)                                                                                                                                                              cGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTGCCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCGTCGCGCCGTTGTGAATGTTGGCTTCCagctcccggcgg                                                                          <  2:439762/251‑12 (MQ=255)                                                                                                                                                                  gAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAcc                                                                      >  2:16278/1‑251 (MQ=255)                                                                                                                                                                       ggCCATCGTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGAcc                                                                 <  1:415675/251‑1 (MQ=255)                                                                                                                                                                                     ccGTAATCCTCACGCACGATCAGGCTGGCGTTGTTATAGGCTTCGGTGTATTCGCGTTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGATCCCGACCGGGTGAGCTTCAgc                                                   <  2:261720/251‑1 (MQ=255)                                                                                                                                                                                      cGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGGGAGCTTCAgcg                                                  <  1:356970/251‑1 (MQ=255)                                                                                                                                                                                      cGTAATCCTCAAGCACGATACGGCCGGCGTTGGTATCGGCTTCGGTGTATTCCCGGATTAATTTTTCCTTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTAAGTACCGGCACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTACAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCTTCGCCCAGCGGAACCCGCCCGGGAGAGCTTCAgcg                                                  >  2:233800/1‑251 (MQ=255)                                                                                                                                                                                       gTAATCCTCCCGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGAATATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATAc                                                                                                                                                                                                                  >  2:434915/1‑90 (MQ=255)                                                                                                                                                                                       gTAATCCTCACTCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATAc                                                                                                                                                                                                                  <  1:434915/90‑1 (MQ=255)                                                                                                                                                                                       gTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCATTGTCAGTACCGGAACGAATAGGGGCATCGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGGTGTGAATTTTGGCTTACATCGCCCAGCGGAACCCGCCCGGGTGAGCTTCAtcgg                                                 >  2:345835/1‑251 (MQ=255)                                                                                                                                                                                               cacgcacgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGCCAGGAACGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGCATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCg                                         >  1:62833/1‑251 (MQ=255)                                                                                                                                                                                                      gATCAGGCTGGCGTTGGCATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCCATGTCAGTACCGGAACGCATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGCGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATc                                   >  2:433798/1‑250 (MQ=255)                                                                                                                                                                                                         cAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATcacca                               <  1:415777/251‑1 (MQ=255)                                                                                                                                                                                                            gCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCacgc                            >  2:145610/1‑250 (MQ=255)                                                                                                                                                                                                                    tGGTATAGGCTTCGGTGTATTCGCGGTTTGCTTTTTCATTGTTCAGCAGGTACAGCACTACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGATGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTg                    >  2:71068/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTATAGGCGTCGGGGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCGACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCAGCGCCCAGCGGAACCCGACCGGGTGAGCTGCAGCGGCGTTACCGCCCATCACCACGACGAGGTTg                    <  2:56446/250‑1 (MQ=255)                                                                                                                                                                                                                            gCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCtt            >  1:190826/1‑251 (MQ=255)                                                                                                                                                                                                                                cGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAAAGCAATACGCCTGACAGGAAAGCAATGGCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATg        <  2:352266/251‑1 (MQ=255)                                                                                                                                                                                                                                   tgtATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGCTCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCg     >  2:380406/1‑251 (MQ=255)                                                                                                                                                                                                                                   tgtATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACAGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCg     >  1:55079/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGAcc  >  1:320654/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 TCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATGTCGACC  >  NC_000913/4084693‑4085172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑