Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,956,003 G→A D225N (GAT→AAT)  ilvA → threonine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,0030GA95.5% 347.8 / ‑6.6 115D225N (GAT→AAT) ilvAthreonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base A (38/68);  total (45/70)
Fisher's exact test for biased strand distribution p-value = 6.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAAC  >  NC_000913/3955757‑3956242
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
aTCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGACAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑GTGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATgg                                                                                                                                                                                                                                              >  1:103130/1‑250 (MQ=255)
    aaatGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGTCCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGGAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGt                                                                                                                                                                                                                                          <  2:320857/248‑1 (MQ=255)
     aCTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTc                                                                                                                                                                                                                                         <  1:376499/251‑1 (MQ=255)
           acaGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCgg                                                                                                                                                                                                                                   <  1:321383/251‑1 (MQ=255)
                agcagGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCa                                                                                                                                                                                                                                        <  1:301690/241‑1 (MQ=255)
                agcagGGGTTAACCTGGGTGCCGACGTTCGACCATCCGATGGCGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCCAACAACTG‑ATGCCGCAAATCAAAGAGATCGCCGGAGAAGAGGGAGACTCCGCCCGCCTGAAAGCCGGGCGGGATGCggggcaagataggga                                                                                                                                                                                                                               >  2:301690/1‑238 (MQ=255)
                      ggTTCCCCTGGGTGCCGCCGTTCGACCATCCGAGGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCcg                                                                                                                                                                                                                        <  2:83164/251‑1 (MQ=255)
                       gTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCCAAGTGATCGCCGTAGAAGCGGAAGGCTCCGCCTGCCTGGAAGCAGGGCTGAATGCGGGGGCTCCGGTTGGACTGCcgc                                                                                                                                                                                                                       >  2:220721/1‑251 (MQ=255)
                         tCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTAGACCGCGTATTTGTGCCCGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGCTCAAACAACTG‑ATGCCGCAAATAAAAATGGTCGCCGTCGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTTAATGAGGGTAATCCGGTTGATCTGCcgcgc                                                                                                                                                                                                                     >  1:283731/1‑251 (MQ=255)
                          ccccTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCcgcgcg                                                                                                                                                                                                                    <  1:134933/249‑1 (MQ=255)
                           aCCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCCAACAACTG‑ATGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGGAGACCCCGCCCGCCTGAAAGCAGCGATGAATGCggggcctcccggtggtacgccgcgcgg                                                                                                                                                                                                                   >  2:353325/1‑227 (MQ=255)
                            ccTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑GTGCCGCCAAACAAAGTGGTCGCCGTAGAAGCGGAAGACTCCGCCCGCCTTGAAGCAGCCCTGAATGCGGGGCATCCGGGTGGTCTGCcgcgcgcg                                                                                                                                                                                                                  >  2:237094/1‑249 (MQ=255)
                                     cgccgTGCGACCCTCCGATGGCGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTTGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTAttt                                                                                                                                                                                                         <  2:103130/251‑1 (MQ=255)
                                        cgTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGGGGCGGTGCTGATTAAACAACTG‑AAGCCCCACATCCAAGAGATCGCCGTAGAAGAGGAAGACTCCGCCTCCCTGCCAACAGCCCTGAATGCGGGGCCTCCGGTTGATCTGCCCCGCGTaagggccgttgct                                                                                                                                                                                                      >  2:120800/1‑239 (MQ=255)
                                           tCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGACCTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑AGGCCGCAAATCAAAGTGATCGCCGGAGAAGAGGAAGACTCCGCCTGCCCGAAAACAGCGCTGCAAGCGGGTCATCCGGTTGGTCTGCCGCGCGTAGGGCTCTTTGCTGa                                                                                                                                                                                                    >  2:398319/1‑250 (MQ=255)
                                            cGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAg                                                                                                                                                                                                  <  1:426852/251‑1 (MQ=255)
                                            cGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAg                                                                                                                                                                                                  <  1:296455/251‑1 (MQ=255)
                                             gACCATCCGCTGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAgg                                                                                                                                                                                                 <  2:101450/251‑1 (MQ=255)
                                             gACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAAATG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGGGGGAGACTCCGCCCGCCCGAAAGCCGCGCTTGATGAGGGGCCTCCGGTTGATCTTCCGCGCGTAGGGCTATTTGCTTAAgg                                                                                                                                                                                                 >  1:454527/1‑251 (MQ=255)
                                               ccATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGTTCCAACAACTG‑ATGCCGCCAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTTATCTGCCGCGCGGAGGGCTATTTTCTGAAGGcc                                                                                                                                                                                               >  1:108353/1‑250 (MQ=255)
                                               ccATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCg                                                                                                                                                                                               <  1:250572/251‑1 (MQ=255)
                                                         gTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTaaa                                                                                                                                                                                     <  2:260130/251‑1 (MQ=255)
                                                          tGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCCAACAACTG‑ATGCCGCAAATCCAAGGGATCGCCGTAGAAGCGGGAGACTCCCCCTGCCTTAAAGCAGCCCTGCATGCGGGTAATCCGGGTGATCTGCCGCGCGTAGGGCTATTTGATGGAGGCGTAGCGGGaaa                                                                                                                                                                                     >  2:414763/1‑250 (MQ=255)
                                                              tGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCCGCAGGACGCCCCTCTCGACCGCGTATTTGTGGCAGGCGGCGGCGGCGGTGTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGc                                                                                                                                                                                 <  2:114511/250‑1 (MQ=255)
                                                                ccGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGc                                                                                                                                                                                                                                              <  2:352480/187‑1 (MQ=255)
                                                                ccGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCCGAATGCGGGTCATCCGGTTGATCTGCCGGGCGGAGGGCTTTTTGCTGAAGGCGTAGCGGTAAAACGCATc                                                                                                                                                                              >  1:408492/1‑251 (MQ=255)
                                                                ccGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGc                                                                                                                                                                                                                                              >  1:352480/1‑187 (MQ=255)
                                                                      aaGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCTGTGAc                                                                                                                                                                        <  2:235307/251‑1 (MQ=255)
                                                                      aaGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGAc                                                                                                                                                                        <  1:56204/251‑1 (MQ=255)
                                                                           ccgctggcgccggAACTGCTCCAGCAGGACGCCCATCTCTACCGCGGATTTGTGCCAGGCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTTATCAAACAACTG‑AGGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACGCCGGCGGCCTGAAAGCAGCGCTTAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTTCTGAAGGCGTAGCGTTAAAACGCATCGGTGACGAAAc                                                                                                                                                                   <  2:263761/250‑1 (MQ=255)
                                                                             gctggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGACGACTCCGCCTGCCTGAAAGCAGCGCTGACTGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCt                                                                                                                                                                 >  1:78211/1‑251 (MQ=255)
                                                                                ggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGGTCGCCGTAGAAACGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGAAGCGGGTCATCCGGGTGTTCTGCCGCGCGTAGGGCTATTTTGTGAAGGCGGTGCGGGTAAACGCATCGGGGACGAAAACTTcc                                                                                                                                                              >  2:226794/1‑251 (MQ=255)
                                                                                ggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTcc                                                                                                                                                              <  1:315228/251‑1 (MQ=255)
                                                                                ggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGGGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTcc                                                                                                                                                              <  1:449609/251‑1 (MQ=255)
                                                                                     tggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTCTTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGTTGCTGATCAAACCACTGGAT‑CCGCAAATCAAAGTGATCGCCGCAGAAGCGGAAGACACAGCCTGCCTGAAAGCAGCGCAGGATGCGGGGCACCCGGGTGATCTGCCGCGCGTAAGGCTATTTTCTGAAGGCGGAGCGGTGAAACGCCACGGCGGCGAAACCTTCCGtttt                                                                                                                                                         >  2:451677/1‑250 (MQ=255)
                                                                                            gCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCa                                                                                                                                                    <  1:381128/249‑1 (MQ=255)
                                                                                                  gcagGACGCCCATCTCGACCTGGTAGTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTTGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGAGTGCCGTAGAAGCGGAAGACTCCGCGTGCCGTAAAGCAGCGCTGAATGCGGGTCAGCCGGTTGATCTGCCGCGCGTGGGGCTATTTGCTTAAGGCGTAGCGGTAAAACGCATCGGTGACGAAATCATCCGTTTATGCGAGGAGTAtc                                                                                                                                            <  2:412275/251‑1 (MQ=255)
                                                                                                   cagGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGTGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGGTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAGCCGTCCGTTTATGCCAGTAGTAtct                                                                                                                                           <  2:54180/251‑1 (MQ=255)
                                                                                                   cagGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGAGCTGATCAAACAAATA‑AAGCCACCAATCAAAGCGATCACCGTAGAAAGGGAAAACTTCGCCTGCCTGAAAGCAGGGCTGAATGCGGGTCCTCCGGATGATCTGACGCGCGTAGAGCTATTTGCTGAAGGCGTCACGGTAAAACGCATAGGTGGAGAAACCTTCCGCTTATGCCAGGAGGAtct                                                                                                                                           >  1:280723/1‑251 (MQ=255)
                                                                                                     ggACGCCCATCTCGACCGCGTATGTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTcg                                                                                                                                         <  1:158832/251‑1 (MQ=255)
                                                                                                        cGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTcgacg                                                                                                                                      <  1:226794/251‑1 (MQ=255)
                                                                                                           ccacctcGACCGCGTATTTGTGCCAGGCGGCGTCGGCGGTATGCCTGCTGGCGTGGCGGTGCTGATCAAACAACTT‑ATGCCGCAACTCAAAGTGATCGCCGTAGAAGCGGAAGTCTCCGCCTGCCTTAAAGCAGCGCTGAATGGGGGTCATCCGTTTGATCTGCCGCGCGTAGGGCTAGTTGCTGAAGGCGTTGCCGTAAAAGGCATCGGTGACGAAACCTTCCGTTTATGTCAGCTGTATCTCGACGAca                                                                                                                                   <  2:55773/247‑1 (MQ=255)
                                                                                                                 cGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCGGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACAtcatca                                                                                                                             <  2:108353/251‑1 (MQ=255)
                                                                                                                          atgtgtgCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATa                                                                                                                    <  1:451677/248‑1 (MQ=255)
                                                                                                                             tgtgCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAACCAAATG‑ATGCCGCAACTCAAAGTGGTCGCCGTAGAAGCGGAAGACTCAGCCTGCCTGCAAGCAGCGCTGAACGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGGCAAACGCATCGGTGACGAAACCTTCCGCTTATGCCAGGAGTATCTCGAAGACCTCATCACCGTCGATaacg                                                                                                                 >  1:278594/1‑248 (MQ=255)
                                                                                                                              gtgCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcg                                                                                                                 <  2:350178/250‑1 (MQ=255)
                                                                                                                              gtgCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAA‑TCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgat                                                                                                               <  1:172422/251‑1 (MQ=255)
                                                                                                                               ggctaGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACACCTG‑ATGCCGCAAATCAAAGTGATCCCTGTAGAAGCGGATGACTCCGTCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGATATTTGCTGAAGGCGTAGCGGTAAAACGCTTCGGTGACGAAACCTTCCGTTTATGTCAGGAGTATCTCGACGGCATCATCACCGTCGATTgcgag                                                                                                               <  2:423037/247‑2 (MQ=255)
                                                                                                                                  cAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATTGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTTAATGCGGGTCATCCGGTTGATCTGCCGCTCGTAGGGCTATTTGCGGAAGGCGTAGCGGTAAAACGCAGCGGTGACGAAACCTTCCGTGTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgatgcg                                                                                                            <  1:304903/251‑1 (MQ=255)
                                                                                                                                      cggcggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATc                                                                                                         <  1:138956/250‑1 (MQ=255)
                                                                                                                                      cggcggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATc                                                                                                         <  1:386703/250‑1 (MQ=255)
                                                                                                                                      cggcggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGAATCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGGCATCCGGTTGGTCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAAACCAACGGGGACGAAACCTTCCGTTTATGCCAGGGGTATCTCGACGACATCATCCCCGTCggtaaccgagcgatct                                                                                                        >  1:361554/1‑236 (MQ=255)
                                                                                                                                      cggcggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAAGCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCt                                                                                                        <  1:325914/251‑1 (MQ=255)
                                                                                                                                         cggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCtgtg                                                                                                     <  1:353325/251‑1 (MQ=255)
                                                                                                                                             ggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTgcggc                                                                                                 <  2:116097/251‑1 (MQ=255)
                                                                                                                                                ggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGAt                                                                                              <  1:406980/251‑1 (MQ=255)
                                                                                                                                                     ggcggcgggcgttgcgGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTAGGCCAGGAGTATCTCGACGACATCATCGCCGTCGATAGCGATGCTATCTGTGCGGCGATGAA‑gg                                                                                        <  2:286713/244‑1 (MQ=255)
                                                                                                                                                     ggCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑AGGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGGAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGGGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCGGTGCGGCGATGAA‑g                                                                                         <  2:223240/250‑1 (MQ=255)
                                                                                                                                                         gctgGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGAtt                                                                                     <  1:237094/250‑1 (MQ=255)
                                                                                                                                                         gctgGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGAt                                                                                      <  2:217635/249‑1 (MQ=255)
                                                                                                                                                                gggcgGTGCTGGTCAAACAACGG‑ATGCCGCAAAGCAAAGTGATCTCCGTAGAAGCGGATGCCTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCTGCCTGTTGATCTGCCGCGCGTAGGTCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGACACCTTCCGTGTATGCCAGGAGTATCTCGACGAAATCATCACCGTCGATAGCGAAGCGTTCTGTTCGGCGATGAA‑GGATTTCTTCGaa                                                                             <  2:283731/250‑1 (MQ=255)
                                                                                                                                                                 ggcgGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAg                                                                            <  1:111915/251‑1 (MQ=255)
                                                                                                                                                                   cggagCTGATCAAACAACTG‑ATGACGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGCATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGAt                                                                          <  2:221826/247‑1 (MQ=255)
                                                                                                                                                                      tGCTGATCACACAACTG‑ATGCCGAAAATCAAAGAGCTCGACGCAGAAGCGGAAGACTCCGCCAGCCTGAAAGCAGCGATGAAGGCGGGTCCTCCGGTTGATCTGCCGCGAGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCAGTACGGCGATGAA‑GGATTTCTTCGAAGAtg                                                                         >  2:308745/1‑249 (MQ=255)
                                                                                                                                                                          gATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTgcgcg                                                                   <  1:241964/251‑1 (MQ=255)
                                                                                                                                                                          gATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTgcgcg                                                                   >  2:123191/1‑251 (MQ=255)
                                                                                                                                                                             cAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCgg                                                                >  1:72055/1‑251 (MQ=255)
                                                                                                                                                                               aacaacTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGCCTCCGCCGGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTg                                                              <  2:349877/251‑1 (MQ=255)
                                                                                                                                                                               aacaacTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTg                                                              <  1:398319/251‑1 (MQ=255)
                                                                                                                                                                                  aacTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCg                                                           >  1:122460/1‑251 (MQ=255)
                                                                                                                                                                                     tG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGa                                                         >  2:234256/1‑250 (MQ=255)
                                                                                                                                                                                      g‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAAc                                                       <  1:187156/251‑1 (MQ=255)
                                                                                                                                                                                          gCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCct                                                    <  1:234256/251‑1 (MQ=255)
                                                                                                                                                                                             gCAAATCAAAGTGATCGCAGTCGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCCGGAGTATCTCGGCGACATCCTCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTg                                                 >  2:250630/1‑251 (MQ=255)
                                                                                                                                                                                                 ttCAAAGAGATCGCCGTAGAAGCGGAAGACTCCTCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAATGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTACGTTTATGCCAGGAGTATCTCGACGACATCATCACCGCCGAAAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCAAATAGGTGCGCGCGGTGGCGGTACCCTCTgtgtc                                             >  2:19191/2‑247 (MQ=255)
                                                                                                                                                                                                   cAAAGTGATCGCCGTAGAAGCGGAAGGCTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGgcgcg                                            <  1:463927/250‑1 (MQ=255)
                                                                                                                                                                                                   cAAAGAGATCGCCGTAGCAGCGGCAGACTCCGCCTGCATGAAAGCAGCGATGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTCGGGCTATTTGCTGAAGGCGAAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGCAGATGTGCGCGCGGTGGCGGAACCCTCTGgcgcgc                                           >  2:238693/1‑251 (MQ=255)
                                                                                                                                                                                                    aaaGTGATCGCCGTAGAAGCGGAAGACGCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgct                                          <  1:300417/251‑1 (MQ=255)
                                                                                                                                                                                                     aaGTGATCGCCGTAGACGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGGGGTAAAACGCACCGGTGCCGAAACCTTCCGTTTTTGCCGGGAGAATCTGGAGGACATATTCCCCGCCGATAGCGATGGGATTGGGGGGGCG‑TGAAAGGATTTATTCGAGGATGGGCGCGCGGGGGCGGACCCCCCTggggggtgg                                         >  1:139324/1‑244 (MQ=255)
                                                                                                                                                                                                     aaGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctg                                         >  2:345601/1‑251 (MQ=255)
                                                                                                                                                                                                      aGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGCAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctgg                                        >  1:341841/1‑251 (MQ=255)
                                                                                                                                                                                                      aGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctgg                                        >  1:209166/1‑251 (MQ=255)
                                                                                                                                                                                                       gTGATCGCCGTAGAATCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCAGCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGAGCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgctctggc                                       <  2:200083/251‑1 (MQ=255)
                                                                                                                                                                                                            cGCCGTAGAAGCGGAAGACTCCGCCTGCCGGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGcgctggcgctgg                                  <  1:19416/251‑1 (MQ=255)
                                                                                                                                                                                                             gCCGTAGAAGCGGAAGACGCCGCCGGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGAGCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGGGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCgctggcgctggc                                 <  2:232210/251‑1 (MQ=255)
                                                                                                                                                                                                               cggaGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCAGCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCGGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCgg                               <  2:350008/248‑1 (MQ=255)
                                                                                                                                                                                                                gTAGAAGCGGACGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTAATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAC‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCggg                              >  1:45413/1‑251 (MQ=255)
                                                                                                                                                                                                                 tAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCggg                              >  2:453417/1‑250 (MQ=255)
                                                                                                                                                                                                                   gAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCTGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                           <  1:87945/251‑1 (MQ=255)
                                                                                                                                                                                                                    aaGCGGAAGACCCCCCCTTGCTGAAAGCAGGGCTGAATGGGGGTCATCCGGGTGATCTTCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATg                          <  2:139324/251‑1 (MQ=255)
                                                                                                                                                                                                                       cGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGaaa                       <  2:112678/251‑1 (MQ=255)
                                                                                                                                                                                                                         gAAGACTCCGCCTGACTGAAAGCAGAGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAACACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGGATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGGATGaaac                      >  2:419646/1‑249 (MQ=255)
                                                                                                                                                                                                                                ccGCCTGCCGGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGAGTTATTCGAAGATGTGCGCGCGGTTGCAGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATc              <  1:169772/251‑1 (MQ=255)
                                                                                                                                                                                                                                   cctgccagAAAGCAGCGCTGAATGAGGGTCATCCAGTTGATCTGCCGCGCGTAGGGCTATTTGCTGACGGCGTAGCGGTAAAACGCATCGGTGACGACACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATCGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGGATGAAAACATATATCGcc           >  2:53515/1‑251 (MQ=255)
                                                                                                                                                                                                                                    ctgcctgAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGCAGGCGTAGCGGTCAAACGCCTCGGTGACGAAACCTTCCGATTATGCAAGGAGTATAACGCCGACCTCATCCCCGCCGATCGCGATGCGATCTGTGAGGCGATGAA‑GGCTTTATTCGACGATGTCCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAAGGAAAAATATATTGccc          >  1:432745/1‑251 (MQ=255)
                                                                                                                                                                                                                                    ctgcctgAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGa                                                                           <  2:172753/186‑1 (MQ=255)
                                                                                                                                                                                                                                    ctgcctgAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGa                                                                           >  1:172753/1‑186 (MQ=255)
                                                                                                                                                                                                                                     tgcctgAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGGATGAAAAAAAATATCGCCCt         >  2:407555/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGGAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGGTAGCGATGCGATCTGGGCGGCGAGGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGGGCGCTGGCGCTGGCGGGAAGTAAAAAATATATCGCCCTGcaca    <  2:417470/251‑1 (MQ=255)
                                                                                                                                                                                                                                            aaGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCacaac  >  1:159260/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
ATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTG‑ATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAA‑GGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAAC  >  NC_000913/3955757‑3956242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑