Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,099	C→T	intergenic (‑130/‑223)	yifB ← / → ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,099	0	C	T	100.0%	114.9 / NA	36	intergenic (‑130/‑223)	yifB/ilvL	putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

CCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTG  >  NC_000913/3949864‑3950342                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ccaccaTCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGTCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTACTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCCAGTGAAGTTGAGTTGTTCTGTCGGTGGGATGTTGTTCGCAAAAATGCAGc                                                                                                                                                                                                                                      >  2:272594/1‑251 (MQ=255)            aaGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCTGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATGCGTGAATTGTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTGGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGAt                                                                                                                                                                                                                           <  2:221877/251‑1 (MQ=255)            aaGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGAt                                                                                                                                                                                                                           <  1:129785/251‑1 (MQ=255)                                   atGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAacaaca                                                                                                                                                                                                    <  1:307360/251‑1 (MQ=255)                                     gTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCCCCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTGACTTGTGCAAGTGAAGTTTAGTTGTGCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACAtt                                                                                                                                                                                                  <  1:272594/251‑1 (MQ=255)                                       aCCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCa                                                                                                                                                                                                <  1:302555/251‑1 (MQ=255)                                         cTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGACGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCAt                                                                                                                                                                                               >  2:296299/1‑250 (MQ=255)                                            aaCAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGAAATAAGCCCTCCTTGAGTCACCATGATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATATTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTg                                                                                                                                                                                           >  2:357445/1‑251 (MQ=255)                                                  gATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAGCATTCATACTGAAAtt                                                                                                                                                                                      <  1:373116/250‑1 (MQ=255)                                                  gATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTg                                                                                                                                                                                     >  1:45508/1‑251 (MQ=255)                                                       ggggCGCAGTTACTCCCAGGTCTGCGCGGGTAGGAACAATTGACAGTGACATAAGCCCTCCTTTAGTCACCATTATGTGCATAAGATATCGCGGCTGTAGCCCGCTAATTCGTGAATGTTAGTTGCTGATTCCTGGTTATTTGTGCAAGTGAAGTTGAGTTATTCTGGAAGTGGAATGATGTTCGCAAAAACCCATCTAACAAGGTATGAACTACGAGGAAGGGAACGTCATTCAGCCTCAAATCGAAttt                                                                                                                                                                                <  2:191888/251‑1 (MQ=255)                                                             cATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtca                                                                                                                                                                          <  1:256296/251‑1 (MQ=255)                                                             cATTTAATCCCAGGGCTGCGCGGGTATGAACAATTGCCAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGCATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACACCATTCATACTGAAATTGAATTTTTTtc                                                                                                                                                                           >  2:287131/1‑250 (MQ=255)                                                                ttACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtcact                                                                                                                                                                        <  1:296299/250‑1 (MQ=255)                                                                     cccAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGGCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtat                                                                                                                                                                  <  2:51061/251‑1 (MQ=255)                                                                                    gTATGAACAATTGACAGTTACATAAGCCCTCCTTGAGTCACCATTATGGGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCGGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGGCAAAGGAGGAACTACGAGGAAGGTAACAACATTCATACTGAAATTGAATTTTTGTCACGCACGATGTTATTTTTaaaaaa                                                                                                                                                   <  2:211092/251‑1 (MQ=255)                                                                                    gTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGCCAAGGGATGAACTACGAGGGAGGGACCAACATTCATAATGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaaa                                                                                                                                                    >  2:187276/1‑250 (MQ=255)                                                                                                   aGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCCTAAGATATCGCGGCTGTAGCCCGTTAATTCGTGAATTTTATTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTAGAGTTGTTCTGTCGGTTGAATGATGTTCGCAAAAATGCAGCTGACAAAGGATGAACTACTAGGAAGGTAACAACATTCATAATGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACACCAATTTATAttt                                                                                                                                    <  1:265369/251‑2 (MQ=255)                                                                                                   aGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGGTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                                                                                                                                    <  1:265363/251‑1 (MQ=255)                                                                                                      gACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaaa                                                                                                                                 <  1:417808/251‑1 (MQ=255)                                                                                                           aaGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat                                                                                                                             <  1:23915/250‑1 (MQ=255)                                                                                                           aaGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCACGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAATTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat                                                                                                                             <  1:86315/250‑1 (MQ=255)                                                                                                           aaGCCCTAATTGCGTCACCATTATCTGCATAAGATCTCGCTGATGTAGCCCGCTAATTCGAGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCACGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACCACATTCATACTGAGATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattttt                                                                                                                            >  2:405415/1‑248 (MQ=255)                                                                                                            aGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta                                                                                                                           <  1:35629/251‑1 (MQ=255)                                                                                                               cctcctTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAAATACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc                                                                                                                        >  1:237229/1‑251 (MQ=255)                                                                                                                 tcctTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTCTTTGTGCCAGTGAAGTTGAGTTGTTCTGGCGGTGGGATCATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGCAGGGAACAACATTCCTACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGc                                                                                                                      >  1:458605/1‑251 (MQ=255)                                                                                                                      gAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca                                                                                                                  <  1:199052/250‑1 (MQ=255)                                                                                                                                tATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATGATTAATCGCATCATAAAAATCgg                                                                                                        <  1:124679/250‑1 (MQ=255)                                                                                                                                                       gTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt                                                                                  <  1:346840/249‑1 (MQ=255)                                                                                                                                                            ccGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGGGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa                                                                            >  1:284872/1‑250 (MQ=255)                                                                                                                                                                   ttCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGAAAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCAATCCCTATTTTATTTTTAAAAAACAACAATTTATATTGATATTATTAAACGCCTCATAAAAATCGGCCAAAAAATATCCTGTACTATTTACAAAACCTATgg                                                                    >  1:458080/1‑251 (MQ=255)                                                                                                                                                                              tAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAAGGCAGCGGACAAAGGATGAACTCCGAGGGAGGGAACAACATTCCTACTGAAATTGAATTTTTTTCACTCACTATTTTCTTTTTAAAAAACAACAATTTATATTGCAATTATTAAACGCATCATAAAAATCGGCAAAAAAATATCTTGTAATATTTACAAAACCTATGGTAACTCttt                                                           >  2:465312/1‑249 (MQ=255)                                                                                                                                                                                         tCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCg                                              >  1:248658/1‑251 (MQ=255)                                                                                                                                                                                                    tgtgCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCCTACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAAATCTTTAGGCATTCCTTCGACCACGATGc                                    >  2:431742/1‑250 (MQ=255)                                                                                                                                                                                                                        gttCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTATGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGc               <  1:357445/251‑1 (MQ=255)                                                                                                                                                                                                                                      atgatgTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTg  >  1:338211/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    CCACCATCGTTAAGCCGGGTAGACCTTTACTGATATGTACCTCAACAGTGATCGGGGGCGCATTTACTCCCAGGGCTGCGCGGGTATGAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTG  >  NC_000913/3949864‑3950342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑