Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,909	G→A	A206T (GCG→ACG)	rbsK →	ribokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,909	0	G	A	100.0%	213.6 / NA	62	A206T (GCG→ACG)	rbsK	ribokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/37);  total (25/37)

TGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCG  >  NC_000913/3937664‑3938149                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  tggtggACGCGCAACGTGAGCGTATTGCCACCGCGTCATCATTATTAATGCCGCTAGAATCACCACTCGAAAGTGCGATGGCAGCGGCGAAAATCGCCCATCCACATCAGACTATCGATGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACGGCTGGCGCTGGTGGCCCTTTTTACGCCGAACGAAACGGAAGCAGAAAATCTCTCCGGTATTCGTGTTGAACATGATGAAGTTGCAACGa                                                                                                                                                                                                                                                >  2:175777/1‑249 (MQ=255)     ggaagcggaaattGAGAGTATTGACAACGCGGAAGAATTATTAATGCAGCGGGAATCACCACTCGAAAGTGTGATGGCATCGGAGCAAATCGCCCATAAAAATAAGACTATCGTTGCGCTTAAGCCGGCTCCGGCTCTCGAACTTCCTGACGAACTGCTGGCGCTGGTTGAGATTATTACGCCAAACGAAACGGTAGCAGAAAAGCTTAACGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAggttg                                                                                                                                                                                                                                          <  2:451813/239‑4 (MQ=255)        aGCGCAACGTGAGCGTATTGCCAACGCGTCCGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACAGGAATTCGTGTTGAAAATGATGAAGATGCAACGAAAGCGgcgc                                                                                                                                                                                                                                       >  1:327708/1‑251 (MQ=255)             aaCGTGAGCGTATTGCCAACGCGTCAGCATTATTAATTCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCTCTTAACCCGGCTCCTGCTCGCGAACTGCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATTCAACGAAGTCGGCGCAGGTa                                                                                                                                                                                                                                  <  1:93997/251‑1 (MQ=255)                  gAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGc                                                                                                                                                                                                                              <  2:307080/250‑1 (MQ=255)                   aGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAAGGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCAt                                                                                                                                                                                                                            <  2:217801/251‑1 (MQ=255)                          tGCCAACGAGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTAGCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACGTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGaaaaa                                                                                                                                                                                                                      <  2:32489/250‑1 (MQ=255)                            ccAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGt                                                                                                                                                                                                                   <  1:402325/251‑1 (MQ=255)                                       gCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGCCTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCgtactgt                                                                                                                                                                                                        <  2:163631/251‑1 (MQ=255)                                        cATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCgtactgt                                                                                                                                                                                                        <  2:371100/250‑1 (MQ=255)                                              taATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGAt                                                                                                                                                                                                  <  1:441987/250‑1 (MQ=255)                                               aaTGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTa                                                                                                                                                                                                <  1:86675/251‑1 (MQ=255)                                                   aaGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCAATCAAAATCAGACTATCGTTGCGCTTAACCCGGCTCAGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATAATTACGCCAAACGAAAAGGAAGCAGACAAGCTCACCGGAATTCGTGGTGAAAATAATGAAGATGCAACGCAGGCGGCGCAGGTACTGCATGGAAAAGGTATCCGTACTGTTCAGCTTACttt                                                                                                                                                                                            >  2:3207/2‑251 (MQ=255)                                                    aGCTGGAATCACCACTCGCAAGTGTGATGGCAGCGGCGAAAATCGCCCATCACAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACttt                                                                                                                                                                                            >  1:40955/1‑250 (MQ=255)                                                        gtaaTCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGaa                                                                                                                                                                                       <  2:75674/249‑1 (MQ=255)                                                        ggAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAgg                                                                                                                                                                                         <  1:25608/249‑1 (MQ=255)                                                            tCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAACATAAGCCTATCGTTGCGCTTAACCCCGCTCAGGCTCGCGAACTTCCTGACGAACTGCTGACGCTGGTGGCCATTATTACGCCCAACGAAACGGAAGCAGAAAAGCCCACCGGTATTCGTGTTGAAAACGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGAATCCGGACTGTACTGATTACTTTAGGAAGt                                                                                                                                                                                     >  1:268506/1‑249 (MQ=255)                                                            tCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCg                                                                                                                                                                                   >  1:188729/1‑251 (MQ=255)                                                                      aaaGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATgg                                                                                                                                                                         <  2:347363/251‑1 (MQ=255)                                                                           ttgatggcggcggttaaaagtgctcATCAAAATAAGACTATGGTTGCGCTTATCCCTGGTCCGGCTAGCGAACTTCCTGACGAACTGCTGGCTCTGGTGGACATGATTACGCCAAACGAAACGGAAGCATAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCTGCGCAGGTACTGCATTAAAAAGGTATCAGTACTGTACGGATTCCTTTAGGAAGTCGTGTTGTATTGGCTa                                                                                                                                                                     <  2:331963/226‑1 (MQ=255)                                                                                           aaaTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGCACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGACGATGCAACGAAGGCGGCACAGGTACTGCATGAAAAAGGCATCCGTACTGTACTGATTACTTTAGGACGTCGTGGTGTATGGGCTAGCGGGAATGATGAAGGt                                                                                                                                                    >  2:10664/1‑251 (MQ=255)                                                                                           aaaTCACCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTACGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGAGGACATTATTACGCCAACCGAACCGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGACGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTATGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAggg                                                                                                                                                    >  2:462753/1‑250 (MQ=255)                                                                                            aaTCTCCCATCAAGATAAGACGATCGGTGCGCTTCACCCGGTTCCGGCTCGCGAACTTCCTGACGGACTGCTGGCGCGGGTGGACATTATTACGCCAAACGAAACTGAAGCAGAAAAGCTCACCGTTATTCGTGTTGAAAATGATGAAGATGCAACGAAGTGGTCTCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATGACTTTATTAATTCGTTGTTGATGGGCTAGCGTGATTGATTAAGGTc                                                                                                                                                   <  2:423393/251‑1 (MQ=255)                                                                                             aTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTAGTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCa                                                                                                                                                  <  2:461957/251‑1 (MQ=255)                                                                                                           tAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATTAAGGGCAGCGTGTTCCTGGAt                                                                                                                                    >  2:344272/1‑251 (MQ=255)                                                                                                                  aTCGTTGCGCGTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGt                                                                                                                             <  1:109699/251‑1 (MQ=255)                                                                                                                    cGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGTTGTATGGGCTAGGGTGAATGATGAAGGTCAGCTCGTTCCTGGATTCCGGGTg                                                                                                                            <  2:163210/250‑1 (MQ=255)                                                                                                                    cGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTg                                                                                                                            <  2:128211/250‑1 (MQ=255)                                                                                                                         cgcTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAACCGGAAGCAGAAAAGCTCACCGGAATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGGTTCCGGGTGCAGGCt                                                                                                                      >  2:170534/1‑251 (MQ=255)                                                                                                                          gcTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATGCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑g                                                                                                                    <  1:54734/251‑1 (MQ=255)                                                                                                                             tAACACGGCTCCTGCTCGCGAACTTCCGGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACTAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCCTGGAAAAAGTATCTGTACTGTACGGATTACTTTTTTAAGTCGAGGTGTACGGGTTAGCTTGGATGATGAAGGTCAGCGCGTGCCTGGATTCCGTGTGctggttgt                                                                                                                    <  2:159241/249‑8 (MQ=255)                                                                                                                              aaCCCGACTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCg                                                                                                                 >  1:349207/1‑250 (MQ=255)                                                                                                                                            gcgcgAACGTCCTAACGAACTGCTGGCGCTGGTTGACATTAGTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTTTTGAAAATGAGGAAGATGCAACGAAGGCGGCGCAGGTACTGCAGGAAAAAGGTATCCGTACTGTACTGATTGCTATAGGAAGTCGTGGGGTATGGGCTAGCGTGGATGATGAAGGTCATGGCGTTCCTGGTCTCCGGGTTCACGCT‑gatgggaccagtgctgccg                                                                                                  <  2:220318/250‑19 (MQ=255)                                                                                                                                              gcgAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCa                                                                                                                                                  >  1:406202/1‑202 (MQ=255)                                                                                                                                              gcgAACGTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAATGTATCCGTACTGTACTGAGTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGga                                                                                                <  1:458135/251‑1 (MQ=255)                                                                                                                                               cgAACTTCCTTACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTTACCGTGATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTTCTGATTACTTTAGTAAGTCGTGGTGTATGGGCTAGCGGGAATGATGAAGTGCAGCGCGTTCCTGGATTCCGGGTGCAGGCTTGT‑GATACCATTTCTGCCGgag                                                                                               <  2:428839/251‑1 (MQ=255)                                                                                                                                                 aaCTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGAt                                                                                             <  2:458313/251‑1 (MQ=255)                                                                                                                                                  aCTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATa                                                                                            <  2:422023/251‑1 (MQ=255)                                                                                                                                                     tCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTACCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATAc                                                                                           <  2:355431/249‑1 (MQ=255)                                                                                                                                                        tGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATtgctgc                                                                                                    >  1:394989/1‑237 (MQ=255)                                                                                                                                                        tGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCGGGATTCCGGGTGCAGGCT‑GTCGATACCATtgctgc                                                                                                    <  2:394989/237‑1 (MQ=255)                                                                                                                                                         gACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACGGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTa                                                                                      <  2:206827/250‑1 (MQ=255)                                                                                                                                                            gaactgccagcgcaggaggccATTATTACGCCAAACGAACCGGAAGCAGAAAAGCTCCCCGGTCTTCGTGTTGCACATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTAACCGAACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCAAGCGGGATTGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTAGATACCATTGTTGCAGGAGATACCTTTAACgg                                                                                  >  2:39650/21‑251 (MQ=255)                                                                                                                                                                 gctgGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATGCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGTTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGc                                                                               <  1:175777/249‑1 (MQ=255)                                                                                                                                                                        cTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATc                                                                                                                                                                                                                >  1:145843/1‑114 (MQ=255)                                                                                                                                                                        cTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATc                                                                                                                                                                                                                <  2:145843/114‑1 (MQ=255)                                                                                                                                                                          ggtggACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATTTGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACg                                                                     <  1:264550/250‑1 (MQ=255)                                                                                                                                                                               aCATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTg                                                               <  2:202489/251‑1 (MQ=255)                                                                                                                                                                                    attaCGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTgg                                                           >  2:276631/1‑250 (MQ=255)                                                                                                                                                                                         gCCAAACGAAACGGAAACAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGCAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGgaagaa                                                      >  2:92168/1‑250 (MQ=255)                                                                                                                                                                                           cAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGaaaaa                                                   <  1:46397/251‑1 (MQ=255)                                                                                                                                                                                                 aaacgGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGCAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTg                                             >  2:228734/1‑251 (MQ=255)                                                                                                                                                                                                 aaacgCAAGCAGAAAAGCTCCCCGGAATTCGTGTTGAAAATGATGAAGATGCAACGAAGGAGGCGCAGGTACTGCATGAACAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGAGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGGTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATAACTTTAACGGTGCGTTAATCAAGGCATTGCTGGAACAAAAACCAtcg                                             >  2:235966/1‑249 (MQ=255)                                                                                                                                                                                                  aacgGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGAGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTAGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGCAAAACCAttcc                                            >  2:311215/1‑249 (MQ=255)                                                                                                                                                                                                          cAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCg                                    >  1:217262/1‑251 (MQ=255)                                                                                                                                                                                                             aaaaGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGAtt                                 >  2:160699/1‑251 (MQ=255)                                                                                                                                                                                                                        ggTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCCTTGCCAGAGGCGATTCGTTTTGccc                       >  2:4334/1‑250 (MQ=255)                                                                                                                                                                                                                                        atgatgAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATTAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCg                                    <  1:32595/221‑1 (MQ=255)                                                                                                                                                                                                                                        atgatgAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAg                                                                                                                                                 >  2:392141/1‑113 (MQ=255)                                                                                                                                                                                                                                        atgatgAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAg                                                                                                                                                 <  1:392141/113‑1 (MQ=255)                                                                                                                                                                                                                                           atgaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGcc   >  1:70426/1‑251 (MQ=255)                                                                                                                                                                                                                                             gaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCg  <  1:160699/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TGGTGGAAGCGCAACGTGAGCGTATTGCCAACGCGTCAGCATTATTAATGCAGCTGGAATCACCACTCGAAAGTGTGATGGCAGCGGCGAAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCT‑GTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCG  >  NC_000913/3937664‑3938149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑