Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,558,291 C→T G8G (GGC→GGT)  rtcR → DNA‑binding transcriptional activator RtcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,558,2910CT97.2% 112.0 / ‑6.3 37G8G (GGC→GGT) rtcRDNA‑binding transcriptional activator RtcR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (22/13);  total (24/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

TGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACGC  >  NC_000913/3558082‑3558536
                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
tGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCGAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTc                                                                                                                                                                                                               <  1:189350/250‑1 (MQ=255)
        tctTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGt                                                                                                                                                                                                      >  1:25943/1‑251 (MQ=255)
                 tgttTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGc                                                                                                                                                                                             >  2:9252/1‑251 (MQ=255)
                    ttGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTTTATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTc                                                                                                                                                                                          >  1:161266/1‑251 (MQ=255)
                     tGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTcc                                                                                                                                                                                         >  2:365107/1‑251 (MQ=255)
                                  aTTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTa                                                                                                                                                                            <  2:48009/251‑1 (MQ=255)
                                                gCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATc                                                                                                                                                              <  1:9252/251‑1 (MQ=255)
                                                 ccAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGACAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCg                                                                                                                                                             >  2:320090/1‑251 (MQ=255)
                                                   aGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATAGCTAAGATGCGTAAAACAGTGGCTTTTGGTTGTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCATTCAGCGCGGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGtt                                                                                                                                                           <  2:397707/251‑1 (MQ=255)
                                                      aacttAAATAAGCAGCTTAATTTTGTAATTCCTTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGt                                                                                                                                                        >  2:406559/4‑251 (MQ=255)
                                                           aaaTAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGGCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcg                                                                                                                                                   <  2:207039/251‑1 (MQ=255)
                                                                                   tCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacgac                                                                                                                            <  2:14122/250‑1 (MQ=255)
                                                                                     aTTGTTTTAAAAAAGATTATATCTTTACGTCCGAAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTATGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGcc                                                                                                                         >  1:379397/1‑251 (MQ=255)
                                                                                       tGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGccc                                                                                                                        <  2:205601/250‑1 (MQ=255)
                                                                                         ttttAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGCAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGCATCGTTGGTCATCGATCGACTGGACTTGTTGCACGACGccctct                                                                                                                     >  2:8635/1‑248 (MQ=255)
                                                                                                      tataTCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGCGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTAtt                                                                                                        >  1:214458/1‑251 (MQ=255)
                                                                                                         atCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGACTTGTTGCACGACGCCCGCTCGCGCTCGCTATTTg                                                                                                      >  2:251842/1‑250 (MQ=255)
                                                                                                                 gTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCACGCTCGCGCTCGCTATTTGAAACGCtt                                                                                              >  1:217993/1‑250 (MQ=255)
                                                                                                                              gaTTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGc                                                                                <  1:164599/251‑1 (MQ=255)
                                                                                                                                             cAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGcc                                                                    >  2:60058/1‑248 (MQ=255)
                                                                                                                                              aaTTTGCCGATAATGAAAGATCGTCTTTTATAAGGATATCTAAGATGCGTAAAAAAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGAGCGGCAGTCAGCGCTGGGCAAAATGGCGTCAGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATGGATCGACTGGGATTGTTGCACGACGCCCGCTCGCGCTCGCTCTTTGAAACGGTTAAACGCGATATCGCCAGCGTTTCGCCAGa                                                                 >  1:91373/1‑250 (MQ=255)
                                                                                                                                                    ccGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGAGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGa                                                           >  1:181578/1‑250 (MQ=255)
                                                                                                                                                       aTAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAgtg                                                       >  1:349596/1‑251 (MQ=255)
                                                                                                                                                          aTGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGAGGCATTAGGTTTTGTCGGTACAGTACCGGATTATGCCGGGCGCCGCAGTCAGCGATGGTCAAAATGGCGTCCGCCACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAAAGCGATATCGCCAGCGTTTCGCCAGAAACAGAAgtggtg                                                    >  1:268211/1‑251 (MQ=255)
                                                                                                                                                               atatCGTCTTTTATAAGGATCTCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGt                                               >  2:86361/1‑251 (MQ=255)
                                                                                                                                                                   cGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTgg                                                                                                                                                         >  1:6530/1‑141 (MQ=255)
                                                                                                                                                                   cGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTgg                                                                                                                                                         <  2:6530/141‑1 (MQ=255)
                                                                                                                                                                        tttATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGAt                                         <  1:260525/248‑1 (MQ=255)
                                                                                                                                                                           ataAGGACATCGAAGATGGGTAAAACAGTGGCTTTTGGTTTTGTGGGTACCGTACTGGATTATGCCGGGCGAGGCAGTCAGAGCTGGTCAAAATGGCGTCCGAAACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCTATCGACTGGAATTGTGGCACGACGCCCGCGCGCGCTCGCTATTTTAAACGATTAAACGCGATATCGCCAGCGTTGCGACAGAAACAGAAGTGGTTAGCGTTGAGATTGAAc                                    <  2:262696/250‑1 (MQ=255)
                                                                                                                                                                              aGGATATCTCAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGc                                 >  2:387345/1‑250 (MQ=255)
                                                                                                                                                                                      tAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGt                        >  1:380778/1‑251 (MQ=255)
                                                                                                                                                                                       aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTg                       <  1:13253/251‑1 (MQ=255)
                                                                                                                                                                                         gATGCGTAAAACAGTGGCTTTTGGATTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTATGTTTATGCCAGCAAGAATCGATGGTCATCGTTCGACTGGAATTGTTGCACGACGCCCGATCGCGCTAGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGCAGTGGTGAGCGATGAGATTGAACTGCATAACACGTggg                     >  1:38167/1‑251 (MQ=255)
                                                                                                                                                                                                aaaaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTCAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGAGGTGAGCGTTGAGATTGAACGGCATAACCCGTGGGTTTTCg               >  2:299/1‑250 (MQ=255)
                                                                                                                                                                                                aaaaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCg               >  2:449917/1‑250 (MQ=255)
                                                                                                                                                                                                  aaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacgac                                                                                                                            <  2:430795/139‑1 (MQ=255)
                                                                                                                                                                                                  aaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacgac                                                                                                                            >  1:430795/1‑139 (MQ=255)
                                                                                                                                                                                                       tggcttttggttttGTCGGTCCCGTACTGGATTATGCCGGGCGCGGCAGTCAGTGCTGGTCAAACTGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGAATGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGGTTTCGAAGAGGTc       >  2:456105/1‑251 (MQ=255)
                                                                                                                                                                                                            tttggttttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACgc  >  1:12304/1‑251 (MQ=255)
                                                                                                                                                                                                            tttggttttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACg   <  1:60058/250‑1 (MQ=255)
                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
TGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACGC  >  NC_000913/3558082‑3558536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑