Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,524,184	C→T	A438A (GCC→GCT)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,524,184	0	C	T	100.0%	348.7 / NA	98	A438A (GCC→GCT)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/65);  total (33/65)

CCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGG  >  NC_000913/3523940‑3524428                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ccAATCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATg                                                                                                                                                                                                                                                 <  1:1537/251‑1 (MQ=255)  cAATCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACGCGGCGCTGGTGTCGATCAATCCGCAAATCGGTGCTGTTATgg                                                                                                                                                                                                                                                <  2:66561/251‑1 (MQ=255)   aaTCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCCTTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGAGTCGTTCAATCCGCAAAACGGTGCTGTTATGgc                                                                                                                                                                                                                                               >  1:19484/1‑251 (MQ=255)   aaTCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGgc                                                                                                                                                                                                                                               <  2:238453/251‑1 (MQ=255)   aaTCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGgc                                                                                                                                                                                                                                               <  1:86178/251‑1 (MQ=255)      ccTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCt                                                                                                                                                                                                                                            <  1:379000/251‑1 (MQ=255)        ccAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGGCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACGCGGCGCTGGTGTCGAGCAATCCGCAAAACGGTGCTGTTATGGCGCTgg                                                                                                                                                                                                                                          <  2:320115/250‑1 (MQ=255)         cAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTg                                                                                                                                                                                                                                           <  1:107354/249‑1 (MQ=255)              aGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGAGGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTg                                                                                                                                                                                                                                    <  2:377337/251‑1 (MQ=255)              aGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTGCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTg                                                                                                                                                                                                                                    <  2:245092/251‑1 (MQ=255)               gCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATTTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCTCAAAACGGTGCTGTTATGGCTCTGGTCGGTgg                                                                                                                                                                                                                                   <  1:392892/251‑1 (MQ=255)                    ggCGATGCTGGCGGACG‑GGTCGACCGTCGCATGGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTg                                                                                                                                                                                                                              <  2:212082/251‑1 (MQ=255)                     gCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGa                                                                                                                                                                                                                             <  1:163923/251‑1 (MQ=255)                     gCGATGCTGGCGG‑CGTGGTCGAACGTCTCATTTAGGATGGAAGGCGTTCGAGGGGCGCGTCCGTAACGTTCTGTTACGCAGCAAGGACTGACGCGGTGTCAAGTGACTGATGTTCTGCAATCGGGTCAGCAAATCTGGGTTGGTTAGGTTGGCTATGCATGGTGGCTGGCAAAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATAAATCCGCAAAACGGTGCTGTTATGGAGTTGGTccagcacgttat                                                                                                                                                                                                                             <  2:17663/251‑12 (MQ=255)                        aTGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGGAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGGTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGAttt                                                                                                                                                                                                                          >  2:86451/1‑251 (MQ=255)                          gCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTc                                                                                                                                                                                                                         <  2:164367/250‑1 (MQ=255)                                  aCG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGc                                                                                                                                                                                                                <  1:324298/251‑1 (MQ=255)                                      ggTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTGTGATTTCAATCAGAGCAAg                                                                                                                                                                                                             <  1:170215/251‑1 (MQ=255)                                       gTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTTCCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGt                                                                                                                                                                                                            <  2:347725/251‑1 (MQ=255)                                          gACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGAAGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGAGGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTAAAACAGAGCAAGttt                                                                                                                                                                                                          >  2:192220/1‑250 (MQ=255)                                          gACCGGCGCGTTGAGGATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTa                                                                                                                                                                                                         <  2:366597/251‑1 (MQ=255)                                           aCCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTa                                                                                                                                                                                                         >  2:353899/1‑250 (MQ=255)                                                cGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACcgc                                                                                                                                                                                                    <  1:241294/250‑1 (MQ=255)                                                cGCAGTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACcgcg                                                                                                                                                                                                   <  1:90543/251‑1 (MQ=255)                                                          atgtaaGGCGTTCGCTGGGGGCGTTCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGATCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGGGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTTCTGTTATGTCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCTCCCAgg                                                                                                                                                                                          <  2:303359/246‑1 (MQ=255)                                                           tGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGc                                                                                                                                                                                         >  2:421341/1‑250 (MQ=255)                                                                 gCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGt                                                                                                                                                                                 <  1:2453/251‑1 (MQ=255)                                                                  cGTTCGCTGGGCGCGTCCTTTGCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTc                                                                                                                                                                                <  1:393400/251‑1 (MQ=255)                                                                  cGTTCGCTGGGCGCGTCCTTGCCGTTCGGATACTCAGCAAGGCCCGACGCCGCGTTACGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTGGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATTAATCCGCAAAACGGTGCTGTTAGGGCGCTGGTCGGTGGCATGGATTTCAATCAGAGCAAGTTTACCCTCGCCACCCAGGCAC‑TGCGTc                                                                                                                                                                                <  1:216479/251‑1 (MQ=255)                                                                   gTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTc                                                                                                                                                                                >  1:46307/1‑250 (MQ=255)                                                                      cGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAgg                                                                                                                                                                             <  1:434571/250‑1 (MQ=255)                                                                       gCTGGTCGCGTCCTGACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGGCAGCAAATCGGGGTGCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTg                                                                                                                                                                           <  2:368080/251‑1 (MQ=255)                                                                       gCTGGGCGCGTCCTTCCCGTTCGGATACTCAGCAAGGACCGACTCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGGGGCGATCAAGCCGCAAAACGGTGCTGTTATGGTGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTg                                                                                                                                                                           <  2:60313/251‑1 (MQ=255)                                                                       gCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAAAGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTg                                                                                                                                                                           >  1:459490/1‑251 (MQ=255)                                                                         tGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGGTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTggg                                                                                                                                                                         >  2:54117/1‑251 (MQ=255)                                                                          gggCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGCTGTGCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACGCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACTCAGGCAC‑TGCGTCAGGTgg                                                                                                                                                                          <  2:231591/249‑1 (MQ=255)                                                                          gggCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTgg                                                                                                                                                                          <  1:326956/249‑1 (MQ=255)                                                                            gcgctgccTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGGTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTc                                                                                                                                                                      <  2:426226/245‑1 (MQ=255)                                                                              gcgTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTcc                                                                                                                                                                     >  1:321324/1‑250 (MQ=255)                                                                              gcgTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCa                                                                                                                                                                    <  1:265698/251‑1 (MQ=255)                                                                              gcgTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCa                                                                                                                                                                    <  1:50021/251‑1 (MQ=255)                                                                                  ccTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGTTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACAt                                                                                                                                                                <  2:362262/251‑1 (MQ=255)                                                                                    ttACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCCGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATc                                                                                                                                                               >  1:296540/1‑250 (MQ=255)                                                                                       ccgttccaataatccgcacggACCGACGCCGCGTAACGTGACCGATGTTCTGCAAACGGGCCAGCAAATCTGGGATCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGGGTCGATCAATCAGCACAACGGTGCTGTTAAGGCGCTGGTCGGTGGCTTTGATTTCTATCAGAGCAAGATTAACAGCGCCAACCAG‑CACCTGCGTCAGGTGGGGTCCAACATCAAAc                                                                                                                                                           >  2:181755/20‑251 (MQ=255)                                                                                         gTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCCC‑TGCGTCAGGGGGGTTCCAACATCAAACCg                                                                                                                                                         >  2:136807/1‑251 (MQ=255)                                                                                           tCGGATACTCAGCAAGAAACGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTAGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCTGGAGGGTTCCAACATCAAACCGtt                                                                                                                                                       >  2:289014/1‑251 (MQ=255)                                                                                             ggATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTcc                                                                                                                                                     <  1:218427/251‑1 (MQ=255)                                                                                             ggATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTcc                                                                                                                                                     >  2:118106/1‑251 (MQ=255)                                                                                              gATACTAAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCct                                                                                                                                                    >  1:353676/1‑251 (MQ=255)                                                                                               aTACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCctc                                                                                                                                                   <  1:213180/251‑1 (MQ=255)                                                                                                 aCTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTa                                                                                                                                                 <  1:294032/251‑1 (MQ=255)                                                                                                         aGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCgcgg                                                                                                                                         <  2:360637/251‑1 (MQ=255)                                                                                                           gACCGACGCCGCGTAAATTGACCGATGTTCTGCAAACGTGTCAGCAAATCGTGGTTCGTCGGTTTGGCGATGCATGGTGGCGGGCACAAGTGCCGGAAGGGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGGTATGGCGCTGGTCGGTGGCGTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCTTCAGGTGGGTTTCAACATCAAACCTTTCCTCGATACCtcggcg                                                                                                                                       <  2:301390/251‑1 (MQ=255)                                                                                                           gACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCgcggcg                                                                                                                                       <  1:353899/251‑1 (MQ=255)                                                                                                             ccGACGCCTCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCATTCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGAt                                                                                                                                     <  1:39814/251‑1 (MQ=255)                                                                                                             ccGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGAt                                                                                                                                     <  1:413999/251‑1 (MQ=255)                                                                                                                  cccgcGTAATGTGACCGATGTTCTGCAATCGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGATGGCACAAGTGCCGGAAGTGAACTCGTCGCTGGTGTCGCTCAATCCGCATAACGGTTCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCATCATCAAACCGGTCCTATACACCGCGGCTAGGGATa                                                                                                                                <  2:329906/250‑1 (MQ=255)                                                                                                                     gcgTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAg                                                                                                                             <  1:95107/251‑1 (MQ=255)                                                                                                                          aaGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCACGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAACCCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTg                                                                                                                        >  1:176426/1‑251 (MQ=255)                                                                                                                                ccGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTg                                                                                                                  <  1:142808/251‑1 (MQ=255)                                                                                                                                       tCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGt                                                                                                            <  1:421341/250‑1 (MQ=255)                                                                                                                                       tCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGt                                                                                                            <  1:285411/250‑1 (MQ=255)                                                                                                                                         tGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATg                                                                                                         >  1:381876/1‑251 (MQ=255)                                                                                                                                            aaccGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGCTGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTg                                                                                                      >  2:138839/4‑251 (MQ=255)                                                                                                                                              aCGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGaa                                                                                                    <  1:214616/251‑1 (MQ=255)                                                                                                                                              aCGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATAAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAGGGTCTGACGAGGGCAGGAATGTTGa                                                                                                     >  2:247925/1‑250 (MQ=255)                                                                                                                                                  gTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGAt                                                                                                <  1:19910/251‑1 (MQ=255)                                                                                                                                                    cAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGAtgt                                                                                              <  2:353676/251‑1 (MQ=255)                                                                                                                                                            cTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGACGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAAttt                                                                                      >  1:398531/1‑251 (MQ=255)                                                                                                                                                              gggTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAAttt                                                                                      <  2:364197/249‑1 (MQ=255)                                                                                                                                                              gggTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAAt                                                                                        <  1:199970/247‑1 (MQ=255)                                                                                                                                                               ggTTCGTCAGGATTGCGATGCATGGTGGGCGGCACAAGTGACGGAAGTGAACTCGGAGCTGGTGTCGCTCAATCCGCAACAAGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTAAATCAGAGCCAGTTTAACCGCGCCACCCCGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTtctc                                                                                   >  2:454498/1‑251 (MQ=255)                                                                                                                                                                    gTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTgg                                                                              <  2:321324/251‑1 (MQ=255)                                                                                                                                                                    gTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTgg                                                                              <  1:129647/251‑1 (MQ=255)                                                                                                                                                                    gTCAGGTTGGAGATGCATGGTGGCTGTCCCAAGCGCAGGAATTGAACTCGGAGCTGGTGTCAATCAATCCGCGAAACGGTGCTGTTATGGAGCTGGTCGGAGGCTTTGACTTCAATAAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGTGTTCCAAAATAAAACCGTTCCTCTACACCGCGGCGATGGATAACGGTATGACGCTGGCATGTATGTTGAACGATGTGCCAATTTCTCGCTgg                                                                              >  2:271062/1‑251 (MQ=255)                                                                                                                                                                      cAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTggg                                                                             >  1:320348/1‑250 (MQ=255)                                                                                                                                                                      cAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACAACGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGGGCAAATTTCTCGCTggg                                                                             >  1:233122/1‑250 (MQ=255)                                                                                                                                                                                 tGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCg                                                                 >  2:72934/1‑251 (MQ=255)                                                                                                                                                                                      ggtggCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCt                                                            <  1:54117/251‑1 (MQ=255)                                                                                                                                                                                         ggctggcACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGAc                                                         <  1:171845/251‑1 (MQ=255)                                                                                                                                                                                         ggctggcACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAAGCCGCAAAACGGTGCTGTTATGGCGCTGGCCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGGATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGAc                                                         <  1:233541/251‑1 (MQ=255)                                                                                                                                                                                              gcACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGc                                                     >  1:94534/1‑250 (MQ=255)                                                                                                                                                                                                     ggCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGGGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTGCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCgaag                                             <  2:413466/250‑1 (MQ=255)                                                                                                                                                                                                        cGGAAGTGAACTCGGCGATGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCgaagcac                                          >  1:380468/1‑248 (MQ=255)                                                                                                                                                                                                         ggAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGCCTGGCAGCCGAAGAACt                                         >  1:368622/1‑251 (MQ=255)                                                                                                                                                                                                            aGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTcac                                      >  1:82163/1‑251 (MQ=255)                                                                                                                                                                                                             gTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTcacc                                     <  1:136807/251‑1 (MQ=255)                                                                                                                                                                                                                   tCGGCGCTGGGGTCGCTCAACCCGCAAAACGGTGCTGTTATGGCGCTGGACGGTGGCCTTGATTGCAATCGGAGCAAGTTTAACCTCGGCTCCCAGGCAC‑TGCGTCAGGGGGGTGCCAACATCAAACCGTTCCTCGACACCGCGGCGATGGATCAAGGTCTTACGCGGGCAAGTAAGTGGAACCATGTGCCAATCTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGc                                <  1:224372/250‑1 (MQ=255)                                                                                                                                                                                                                    cGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAg                              <  2:365480/251‑1 (MQ=255)                                                                                                                                                                                                                     ggCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGt                             <  2:46307/251‑1 (MQ=255)                                                                                                                                                                                                                       cgcTGGGGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTAt                           <  2:94534/251‑1 (MQ=255)                                                                                                                                                                                                                          tGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGGGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCt                        >  1:427757/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCact                >  2:315841/1‑249 (MQ=255)                                                                                                                                                                                                                                     aaTCCGAAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAGTAAGAGCAAGTTTAACCGCGACACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCGGGCCAGTATGTTGAACGATGTGCCAATTTCTAGCTGGGATGCAAGTGCCGGTTGTGACTGGAAGCCGAAGAACTCAACACCGCAGTATGCTGGGCAAATTCg             >  1:342421/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCTCAGTATGCTGGTCCAATTCGCTTAc        <  2:426120/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaCGGTGCTGTTATGTCGCCGGTCGGCGGCTTTGATTGAAAGCAGAGCAAGTTTACCCGCGCCCCCCAGGCAC‑TGCGTCAGGTGGGTGCCATCATCAAACCGTTCCTCTACACCGCGGCGATGGCTAAAGATCTGACGCTGGCAAGTATGTTGAACTAGGTGCCAATTTCCCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCTCCTCAGTATGCTGGTACAAttttctgacgtc     <  2:244432/251‑11 (MQ=255)                                                                                                                                                                                                                                              aaCGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTc     >  1:22727/1‑250 (MQ=255)                                                                                                                                                                                                                                                cGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGGCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTTTCGCTGGTATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAgg  <  1:454498/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACG‑GGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCAC‑TGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGG  >  NC_000913/3523940‑3524428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑