Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,506,393	T→C	D315G (GAC→GGC)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,506,393	0	T	C	100.0%	166.9 / NA	53	D315G (GAC→GGC)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

AGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATG  >  NC_000913/3506170‑3506638                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       agagAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCg                                                                                                                                                                                                                             <  1:5237/251‑1 (MQ=255)           gTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAATAAAGGTAAAGCCCGCAACTGCAt                                                                                                                                                                                                                    >  1:275175/1‑250 (MQ=255)             ataACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATac                                                                                                                                                                                                                  <  2:389715/250‑1 (MQ=255)                 cccGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGTGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAACCCGCAACGGCATACACAcc                                                                                                                                                                                                             >  1:365753/1‑251 (MQ=255)                     ccATCTTAATTGCTGCGAAAATCGCACCGACCCGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGCCGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGTTCGAACGAAGCAGCCAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCCACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGtt                                                                                                                                                                                                         >  2:220140/1‑251 (MQ=255)                           tacttGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATCAACAAAGGTAAAGCCCGCCACTGCATACACACCCGTTGCTAAc                                                                                                                                                                                                   >  2:146931/4‑251 (MQ=255)                                 cggcGAAAATCGAACCGACCAGCGGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCTAACGAAGCAGCAAGACTTCCTCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGGGATAGCCCGCCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCggtg                                                                                                                                                                                             <  2:452020/249‑1 (MQ=255)                                       aaaTCGAACCGACCAGCAGCGCCACTTCTATCAGAATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGAGTCCCAGACATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGCAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGGGGTGGc                                                                                                                                                                                        >  2:441065/1‑250 (MQ=255)                                         aTCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCAGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCCTCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGAAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAAt                                                                                                                                                                                     >  2:177532/1‑251 (MQ=255)                                            gAACCGACCAGCCGCGCCACTTCCATCAGCATATTCATGACGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCCTAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGAGGTGGTGGCAATCa                                                                                                                                                                                   >  1:342127/1‑250 (MQ=255)                                            gAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCaa                                                                                                                                                                                  <  2:194208/251‑1 (MQ=255)                                            gAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGGTGCTAACGCGGTGGTGGCAATCa                                                                                                                                                                                   >  2:14118/1‑250 (MQ=255)                                             aaCCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAAc                                                                                                                                                                                 >  1:369801/1‑251 (MQ=255)                                                           gcCACTTCCATCCGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑Acccc                                                                                                                                                                    >  2:33135/1‑249 (MQ=255)                                                           cccccTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGCCGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAg                                                                                                                                                                  <  2:164073/247‑1 (MQ=255)                                                                      cAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGGCGGAGAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATGCCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCATCGGCACAA‑GCCCCAGTCCGCGCATaa                                                                                                                                                       <  2:417596/251‑1 (MQ=255)                                                                         cATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCTATCGAACGAAGCAGAAAGACTTCCGCCGAAATAAACACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGAAAAGCCCGCAACTGCATACCAACCCGTTTCACACGCGATGGCGGCAATCCACGCCACAACACCCC‑GCCCGCGCATAAAtt                                                                                                                                                    >  2:128617/1‑251 (MQ=255)                                                                           tatTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCt                                                                                                                                                  >  1:357665/1‑251 (MQ=255)                                                                             ttCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCCGCAAGACTTCCGCCGAAATAACCACAGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGCGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGc                                                                                                                                                >  1:180419/1‑251 (MQ=255)                                                                                    cGTTACGGATATTATCCGACGCCTTAAGCACCGCCGGGCCGCGTCCCAGCCATTTGCCGCTCGAACGAAGAAGCACGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTACAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGGGGCACTCAACGGCACAA‑ACCCCAATCCGCGCATAAATTCTGCCAGAgcc                                                                                                                                         >  2:310287/1‑251 (MQ=255)                                                                                     gTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAgccg                                                                                                                                        <  1:441065/251‑1 (MQ=255)                                                                                        aCGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCt                                                                                                                                     <  1:97044/251‑1 (MQ=255)                                                                                          ggATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAACTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTg                                                                                                                                    >  1:281130/1‑250 (MQ=255)                                                                                           gATGTTATCCGACGCATTACGCACCGACGTGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGTCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGAt                                                                                                                                  <  1:254882/251‑1 (MQ=255)                                                                                                     gACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGt                                                                                                                        <  1:303196/251‑1 (MQ=255)                                                                                                     gACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGACCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGt                                                                                                                        <  2:67181/251‑1 (MQ=255)                                                                                                       cGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCAGAAATTGTGCCAGAGCCGCCTGATTAATCAGCGttt                                                                                                                      <  1:333170/251‑1 (MQ=255)                                                                                                            tACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGa                                                                                                                  <  1:437483/250‑1 (MQ=255)                                                                                                                          tAGCGCCCCAGCCATATCCAGATCGAAAGACGCAGCCAGACCTCCGCAGCCATCACCACTGCGCCTAATAACGCTCCCAACATCGCATTCGGCGAGAGCTAGCCCACCGCATAAACAACGGTAAACCCCGCAACTGCCTACACCCCCGTTGCTAACGCGGTGGTGGCAATCAACGGCGCAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGAGTTTGAGATTGTTCCGGCGTTa                                                                                                    >  2:302730/1‑250 (MQ=255)                                                                                                                             cGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCCCTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGcc                                                                                                <  2:286959/251‑1 (MQ=255)                                                                                                                                 ccacccATTGGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGGTTGCGATTGTTCCGGCGTTACGCCTGCg                                                                                            <  2:119462/247‑1 (MQ=255)                                                                                                                                      cATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATa                                                                                                                                                        <  2:293236/186‑1 (MQ=255)                                                                                                                                      cATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATa                                                                                                                                                        >  1:293236/1‑186 (MQ=255)                                                                                                                                               aTCGAACGAAGCAGCAAGACTTCCGCCGAACTAACCACTGCGCCTAATACTGCTGCAACCATCGGATTCGGCGAGAGATAGCCAACAGCATAAACAATGGAAAAGCCCGCCACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCCCAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGAGATTGTTCCGGCGTTACGCCTGCGGCATATGCTTtctc                                                                              >  1:183211/1‑251 (MQ=255)                                                                                                                                                  gaacgaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAg                                                                           <  2:80896/251‑1 (MQ=255)                                                                                                                                                     cgaaGCAGCAAGCCTGCCGCCGAAATAACCCCTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCAGACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAgtgt                                                                        <  2:381795/251‑1 (MQ=255)                                                                                                                                                               aGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAATCAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGac                                                              <  1:277323/251‑1 (MQ=255)                                                                                                                                                               aGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAATGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGac                                                              <  1:356938/251‑1 (MQ=255)                                                                                                                                                                     ccgccgAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTcc                                                        >  1:152889/1‑250 (MQ=255)                                                                                                                                                                     ccgccgAAATAACCACTGCGCCGAAGACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCa                                                        <  2:369801/251‑1 (MQ=255)                                                                                                                                                                      cgccgAAAAAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCCCACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCAc                                                       <  2:259574/251‑1 (MQ=255)                                                                                                                                                                            aaTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAg                                                 <  1:271323/251‑1 (MQ=255)                                                                                                                                                                               aaCCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCa                                               >  2:370722/1‑250 (MQ=255)                                                                                                                                                                                        cccTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCa                                     <  1:52505/250‑1 (MQ=255)                                                                                                                                                                                                     gCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAAcgg                         <  1:33135/250‑1 (MQ=255)                                                                                                                                                                                                         ccATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACggcggc                     >  1:412667/1‑250 (MQ=255)                                                                                                                                                                                                          cATCGGATTCGGCGGGAGATAGCCCACCGCATGAACAAAGGTAAAACCCGCAACTGCATACACACCCGTTGCTAACGCGGCGGCGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTCCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGGGGAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGca                    >  1:229931/1‑250 (MQ=255)                                                                                                                                                                                                           aTCGGATTCGGCGCGCGACAGCCCACCGCCTGACCAAGGGTAACGCCCGCACCAGCATCAAGCCCCGTTGCTACCGCGGTGGAGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTATGCCAGAGCCGCGTGATTACTCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGCTCGACACTTCACTGCCAGCACAAATCTTCATCATGGCAACGGCGGcaat                  >  2:309751/1‑251 (MQ=255)                                                                                                                                                                                                              ggATTCGGCGAGAGATAGCCCACCGCATAGACAAAGGAAAAGCCCGCAACTGCATACACACCCGTTGCAAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACCCTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGcaatcc                >  2:385446/1‑249 (MQ=255)                                                                                                                                                                                                                         gagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGAGGTGGCAATCAACGGCACAA‑ACACCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGttt                                                                                                                      >  2:38630/1‑137 (MQ=255)                                                                                                                                                                                                                         gagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGGTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGttt                                                                                                                      <  1:38630/137‑1 (MQ=255)                                                                                                                                                                                                                           gaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATg  <  1:300339/251‑1 (MQ=255)                                                                                                                                                                                                                           gaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATg  >  1:109585/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       AGAGAATCCGGTATAACCCGCCATCTTAATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAA‑ACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATG  >  NC_000913/3506170‑3506638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑