Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,259,283	G→A	H123Y (CAT→TAT)	tdcG ←	L‑serine deaminase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,259,283	0	G	A	100.0%	140.2 / NA	42	H123Y (CAT→TAT)	tdcG	L‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/23);  total (19/23)

CCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGC  >  NC_000913/3259040‑3259522                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                ccGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCCGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGatgc                                                                                                                                                                                                                                          >  2:54139/1‑248 (MQ=255)   gCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCa                                                                                                                                                                                                                                         >  2:129736/1‑250 (MQ=255)      ttGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAAc                                                                                                                                                                                                                                       <  2:112927/249‑1 (MQ=255)       tgctgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAAc                                                                                                                                                                                                                                       >  2:251488/1‑248 (MQ=255)       tgctgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAAc                                                                                                                                                                                                                                       <  1:251488/248‑1 (MQ=255)         ctgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGCCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGCGGCAACATTTc                                                                                                                                                                                                                                  >  1:249221/1‑251 (MQ=255)              cAGCGCTAGCTCGTTGTGCATTATAAGACCAGATATAGACAGGCGGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTGCGAAAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGAt                                                                                                                                                                                                                             <  1:202997/251‑1 (MQ=255)                  gcTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACCCATTTTCAGCAGTTCACCTGCTGAATGGAAATCGTAAGGTACGAACGTTTCGACATCGAGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGCGTAATAGGTTTTCCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATgg                                                                                                                                                                                                                           >  1:460742/1‑249 (MQ=255)                  gcTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGa                                                                                                                                                                                                                          >  2:295662/1‑250 (MQ=255)                     aGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGa                                                                                                                                                                                                                          >  1:342138/1‑247 (MQ=255)                     aGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAg                                                                                                                                                                                                                        <  1:444627/249‑1 (MQ=255)                     aGCTAGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGAt                                                                                                                                                                                                                      <  1:140691/251‑1 (MQ=255)                       cTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGACGATa                                                                                                                                                                                                                     >  1:384840/1‑250 (MQ=255)                         cgtggtgCATAAGCAGACCAGATATAGACAGGCCGGTGTAATATCACATATGCAGCAGTGCACCTGCTGAGTGGAAATCTTAAGGTACGGACGTTTCGACATCGGGCGACAGGCCGAAGTGTGCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGGGATCCGCATTCCGTTCTCATAGCGAGGCAAAATTTCGGGATGGAAGATAATg                                                                                                                                                                                                                  <  2:243164/247‑1 (MQ=255)                             gttcatcatCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCATCAGTTCACCTGCTGAGTTGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGTCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGTAACATTTCGGGATGGAAGATAATGTTc                                                                                                                                                                                                               <  2:162003/247‑1 (MQ=255)                               gCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGTACGTTCCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAGCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                                                                                                                                                                                                            <  2:108590/251‑1 (MQ=255)                               gCATCATCAGACCAGAGATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                                                                                                                                                                                                             <  2:141173/250‑1 (MQ=255)                                             gATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc                                                                                                                                                                                              <  2:372353/251‑1 (MQ=255)                                              atatAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATca                                                                                                                                                                                             <  1:129736/251‑1 (MQ=255)                                                      aGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATg                                                                                                                                                                                     >  1:209809/1‑251 (MQ=255)                                                                         ttttCAGCAGCTCACATGCTGAGTGGAAGTAGTAAGAAACGGACGTTTCGAAATCGTGCGACAGGCGGAAGGGTTCTTCTTCGACAATAACCACGCAGCCGACAGAGGAATAGGGTTTACTTAATAGCACTTACCGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACCTTTCGGGATGGAAGAGAATGTTCTTTGCTACAGGAAAGTCAACAAGTTGCGCACCAGGTGCAACTGGc                                                                                                                                                                  >  2:378592/1‑251 (MQ=255)                                                                             cagcagTTCTCCTGCTGAGTGGAAATCGTAAGGTACGGATGTTGCGACATCGTGCGACAGGGCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCTCCAGATGCCACTGGCAgcc                                                                                                                                                              <  1:273221/251‑1 (MQ=255)                                                                              agcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgccg                                                                                                                                                             <  1:295662/251‑1 (MQ=255)                                                                                cagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAgccgcc                                                                                                                                                           >  1:313556/1‑251 (MQ=255)                                                                                     cACCTGCTGAGTGGAAAGCGTAAGGTACGGACGTTTCGCCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTGCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTg                                                                                                                                                      <  2:363192/251‑1 (MQ=255)                                                                                                 ggAAATCGTAAGGTACGTACGTTTCGTCAACGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAATCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCCTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTTCGCGTTACTAAc                                                                                                                                          <  1:221302/251‑1 (MQ=255)                                                                                                           aGGTACGGACGTTTCGACAGCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATg                                                                                                                                <  2:27027/251‑1 (MQ=255)                                                                                                                cGGACGTTTCGACATCGTGCGACAGGCCGAAGGGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTGGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAggg                                                                                                                           <  2:229339/251‑1 (MQ=255)                                                                                                                                                tgtTCTTCTTCGACAATCAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTACCTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGtt                                                                                           >  2:219029/1‑251 (MQ=255)                                                                                                                                                         tcGACAATAAACCCGCCGCCGACAGATTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGcc                                                                                    <  1:115413/249‑1 (MQ=255)                                                                                                                                                           gACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGAGAACATCCGGCGGACTGGTTCCTGCCAGTc                                                                                <  1:334159/251‑1 (MQ=255)                                                                                                                                                                     ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCCGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCAATGGAAGCCGACCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCatgatgat                                                                      >  1:88141/1‑251 (MQ=255)                                                                                                                                                                          gccgACAGAGTAATATGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTGGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCtgctgc                                                                                                                                                     <  2:184985/167‑1 (MQ=255)                                                                                                                                                                          gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCCCCAGATGCCACTGGCAGCCGCCCGCTgc                                                                                                                                                     >  1:184985/1‑167 (MQ=255)                                                                                                                                                                                             tttcTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTGGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCGCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCTACATCCGTGGCGTGGCCtt                                               <  2:313556/247‑1 (MQ=255)                                                                                                                                                                                              tACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTATCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTg                                             >  1:290606/1‑251 (MQ=255)                                                                                                                                                                                                       gCTCTTCCTGACCCTTCCAGGCAGTGATCCTCCTTCCGTTCTCATAGCGAGGCAACATTTCGGGTTGGAAGATAAGGTTCTTTGCTACAGGAAAATCAAAAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGCCTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGt                                    <  2:249221/251‑1 (MQ=255)                                                                                                                                                                                                               tGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGCAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGATTCCTGCCAGTCCCATGATGCTGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAAca                             >  1:192646/1‑249 (MQ=255)                                                                                                                                                                                                                 tCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGAGATCTCATCAATGACAACATCCTGCGCACTGTTTCCTGCGAGTCCCATGATGATGGCGACATCAGTGGCATGGCCTTTGCCCGTCAGAGACAACGAcc                          >  1:9875/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGTGATCCGCATTCCGTTCTCATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTAc                     >  2:247847/1‑245 (MQ=255)                                                                                                                                                                                                                                    cGCATTCCGTTCTCAAAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAat       <  2:192646/251‑1 (MQ=255)                                                                                                                                                                                                                                         tCCGTTCACATAGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGCAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTAATCAATGACAACATCCTTCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTAAGTGACAACGACCCGTACAGACCGCCCACAATTTGGc  >  2:296426/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                CCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCTGCGGACTGTTTCCTGCCAGTCCCATGATGATGGCGACATCCGTGGCATGGCCTTTGCCCGTCAGTGACAACGACCCGTACAGATCGACCACAATATGGC  >  NC_000913/3259040‑3259522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑