| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,227,339 | C→T | T369I (ACC→ATC) | ygjI → | putative transporter YgjI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,227,339 | 0 | C | T | 88.9% | 47.2 / ‑3.9 | 18 | T369I (ACC→ATC) | ygjI | putative transporter YgjI |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (9/7); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTT > NC_000913/3227094‑3227545 | tGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGAtcgcc < 1:295693/250‑1 (MQ=255) gtcggcccggTGTACTTTACCGCGATGTTCGGTTCCCTGCTGATGCGGCCCGCAACGCCGGCGAAAATTTTCTTCTCCGAACTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGCTCCAGTTCCTGCTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGCATACTATTATTAATATGCTcgccgc > 2:230775/9‑251 (MQ=255) gtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATg < 1:1828/250‑1 (MQ=255) cGCAACGCCGGTGAAAATTTTCTTCTCCGAACTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGGGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAc < 1:243786/251‑1 (MQ=255) ccGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATCCTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCAc < 1:239588/249‑1 (MQ=255) cccGGAAGGCATCTTTGGTAAGAAAACCGACGAACTGAACGAAACCGGCGTTCCGGCGCGCGCAGCTTGGATCCAGTTCCTGATCGTTATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GTTGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTcc < 1:462760/251‑1 (MQ=255) aGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATgg > 1:82716/1‑251 (MQ=255) cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGACCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTccc > 1:426351/1‑251 (MQ=255) ttGGTAAGAAAACCGTCGAACTGACCGAAAACGGCGTTCCGGCGCGCGCATCGTGGTTCCAGTTCCTGATCGTGATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATGCCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCAGGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCTACGCGATGTCCGTATGGGCta < 2:315354/247‑2 (MQ=255) tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCccgcc > 2:100156/1‑251 (MQ=255) cgggCGCGCGCGGGGTGGTTCCAGTTCTTGATCGCCCCCCCGATGATGTTTACCCCGAGGCTCGTTTCCAATCCCGTGC‑GGATTTTGATGAATACTATTATAAATATGACCGCCGCAGCGTCCAGGCTTCCGCCGTTATTCATCATGCGGGCTTACGTGAATTTCCGCGCAAAATTAGATCCCTTGCCACGCGTTTTCCGTAGGGGCTTCCGCCGCGCCGGTATTATCGTTGTTTCAATGCTGATTGCGat < 1:368642/249‑1 (MQ=255) gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGTTTCCAATCCCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCTTTATTCATCATGCTGGCTTACCTGAATTTCCGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTg < 1:250884/251‑1 (MQ=255) cgcAGCGTGGATCCGTTTCCTGATCGTCATCCCTCTGATGATTATCCCGATGCGCGTTTCCAATACCGTGCAGTATCT‑GATAAATCTTATTATTAATATGATCGCCGCAGCGTCCTTGCTTCCGCCGTTATTCATCATTCTGGCTTACCGGAATTTACGCGCCAAATTAGTTCCCCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCg < 1:84056/251‑1 (MQ=255) ttCCTGATCGACATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCACTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTc > 2:405243/1‑251 (MQ=255) cTGATAGTAATCCCGCTGATGATTATCCCGATGCTCGGTTCAAATACCGTGAAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGCTTTCCGTATGGGCTCCCGCCGCACCGGTATTATAGTTGTTTCAATACTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGAc > 2:274349/1‑251 (MQ=255) tCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAACT‑GATGAATACTCTTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCg > 1:222150/1‑251 (MQ=255) ccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTgg > 1:273020/1‑251 (MQ=255) tccattcccgggCAGGATAT‑TATGAATACTATTATTAATATGACCGCCGCACCGTCCATGCTTCCGCCGTTATTCATCATGCTGGTTTCCCTGAATTTACGCGCCAATTTAGACCCCCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCAtttttt < 1:181798/239‑1 (MQ=255) ggATCT‑GATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTg > 2:93933/1‑209 (MQ=255) | TGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCT‑GATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTT > NC_000913/3227094‑3227545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |