| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,224,895 | C→T | L755L (CTG→TTG) | ebgA → | evolved beta‑D‑galactosidase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,224,895 | 0 | C | T | 98.7% | 248.6 / NA | 74 | L755L (CTG→TTG) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (41/32); total (42/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGT > NC_000913/3224655‑3225139 | aTTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGa < 1:73026/251‑1 (MQ=255) tGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaa > 1:191687/1‑249 (MQ=255) tGACCAAAGATTACCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCac > 1:332631/1‑248 (MQ=255) gACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGa < 2:151365/251‑1 (MQ=255) cAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATc > 1:160244/1‑250 (MQ=255) aaaGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCa > 2:1558/1‑250 (MQ=255) aGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTGCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTGCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGTCTCGCGAGCCAAAGATCAAct < 1:377407/251‑1 (MQ=255) aCCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGcc > 1:334942/1‑251 (MQ=255) gACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACGCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAg < 1:293203/251‑1 (MQ=255) ccccTATCGCCACTTATCAGGTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAAGGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTGCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTGCTTCAAGCCGATGATCGACAACCACAAGCAg < 2:168394/250‑1 (MQ=255) tATCGCCACTTATCAGTTACCGCTGAAGGAAAACACCGAGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGt > 2:307167/1‑251 (MQ=255) aTCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGt > 2:112977/1‑250 (MQ=255) aCTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAgg > 1:126965/1‑251 (MQ=255) aCTTACCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCTTTTCGCACCAAATAATGCTCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCGGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAgg < 2:253364/251‑1 (MQ=255) aTCAGTTCCCGCTGAAGGAAAACCCCGCGCAGCCAGTGCCTGTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCt < 2:124046/250‑1 (MQ=255) aTCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCtg > 1:65679/1‑251 (MQ=255) gTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGc < 1:130810/251‑1 (MQ=255) ttCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAACGATCAACTTCTTCAAGCCGATGATCGACACCCACAAGCAGGAGTACGAAGGGCTGTGGc > 2:184611/1‑250 (MQ=255) ttCCCGCTGAAGGAAAACACCGCGCAACCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGAACGAAGGGCtgtagc > 2:323027/1‑247 (MQ=255) cccGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAc > 1:285576/1‑251 (MQ=255) ccGCTGAAGGAAAACCCCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGTCAAcc < 1:126724/251‑1 (MQ=255) ccGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAcc < 2:65679/251‑1 (MQ=255) gCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCg > 2:333440/1‑250 (MQ=255) gAAGGAAAGCACCGAGCAGCAAGAGCCTTTCGAACAGCATAATGCGCGTCCGATGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTAGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGAGG‑AAACCGGACATCCTGGCAGGTGAAAGGCGAATCGCTGTTGACACGCGAGCCAAAGATCAACTTCTTAAAGACGATGATCGACAACACCAAGCCGGAATACGCAGGGCTGTGGCACCCGAATc > 2:277749/1‑251 (MQ=255) gAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCCCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAAt < 1:137483/250‑1 (MQ=255) aGGAAAACACCGCGCAGCCAGTGCCGTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGGGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTGCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAt < 2:369125/251‑1 (MQ=255) aaaCACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc < 1:332066/251‑1 (MQ=255) aaaCACCGCGCAGCCAGTGCCTTTCGCACCAAAGAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCACGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc < 1:74593/251‑1 (MQ=255) acacCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGAGCGTTTGAGCTGCACCGTGCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAg < 2:383893/251‑1 (MQ=255) gTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGCCTACAACTTCGCGATCACCTTCTCAAACATGAGTGGCAAACCG‑ACATACTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGAACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAAc > 1:214305/1‑250 (MQ=255) gCCTTTCGCACCAAATAATGCGCGTCCGCTGAAGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATc > 1:166418/1‑251 (MQ=255) ccTTTCGCCGCAAAGAAGGCGCGCCCGCTGACGCTGGAAGGCGAGCGTTTGGGCTGCACCGTGCGCGGCTACAACTTCGCGATCACCTTCTCAAAAAGGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCTAATCGCTGTGGACTCGCGAGTCAAAGATCAACTGCGTCAAGCCGATGATCGACAACCACAAGCAGGATTACGAAGGGCTGGGGCAACCGAATCATGTGCATCTCATGCAGGAACATCt < 2:214305/251‑1 (MQ=255) cTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGACTGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGCTGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTg > 1:44677/1‑251 (MQ=255) tttCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCt < 1:111044/249‑1 (MQ=255) tCGCACCAAATAATGCGCGTCCGCTGACGATGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACAG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGTAGATCATGCAGGAACATCTgcgc > 2:147659/1‑251 (MQ=255) ccAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGCTGATCGAAAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCCGGAACATCTGCGCGACtt > 2:273023/1‑251 (MQ=255) aaaTAATGCGCGTCCGCGGACGCTGGAAGACGATCTTTTGAGCTGCCCCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTg < 2:285576/251‑1 (MQ=255) taatGTGCGTCCGCTGACGCTGGAAGCCGATCGTTTGAGCTGAACAGTTAGCGGCTACACCTTCGCGATCCCCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGCATCGCTGTTGACTCGCGAGc > 2:200371/1‑140 (MQ=255) taatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCGTCTCAAAAATGAGTGTCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGc < 1:200371/140‑1 (MQ=255) cgTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAAc > 2:416434/1‑250 (MQ=255) gCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCg > 2:376216/1‑251 (MQ=255) gCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCg < 1:112977/251‑1 (MQ=255) aGACGATCGTTTGAGCTGCACAGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATAAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGACGGGCTGTGGCAACCGACTCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc > 1:380191/1‑251 (MQ=255) aCGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTg < 1:184611/251‑1 (MQ=255) cGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGa < 2:99168/251‑1 (MQ=255) gATCGTTTGAGCTTCACCGTTCGCGGCTACACCTTCTCGATCACCTTATACAACATGCGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCACGCCGCTGATCGCCAACCACCAGCAGGAGCACGACAGGCTGAGGCAACCGAATCCTTTTCAGATCATGCAGGCACATCTGCGCGCCTATACCGCAGACCAGAGCGATGGCGAAGTGCtcat > 2:25321/1‑248 (MQ=255) cgattGAGATGCACCGTTCGCGGCTACAACTTCGCGAGCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTTAATGGCGAATCGCTGTTGACTCCCGAGCCAAAGATCAACTTCTGCAAGCCGATGAGCGACAACCACAAGCAGGAGGACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTGTGCCGTAGAGCAGATCGAGTGTGAAGTGCGGatcat < 2:210227/248‑1 (MQ=255) gAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATACTGGCAGGTGAATGGCGAACCGCTGTTGACGCGCGAGCCAAAGATCAACTTCTTCAAGCCGAGGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGAGCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGc < 2:32801/250‑1 (MQ=255) ccGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGACCATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGtt > 1:373883/1‑251 (MQ=255) tCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAACCCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAtt < 2:44677/250‑1 (MQ=255) cgATCAACTTCTCAAAAATGAGTGGCAAACCG‑ATATCCTGGCAGGTGAATGGCGAATCGATGTTGGCTCGCGAGCCAAAGATCAACTTCTTAAAGCCGATGATCGACAACCACAAGCAGGAGTACGCAGGGCTGAGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGCCTTTGCCTTAGACAAGAGCGATGGTGAAGTGCTGATCATCAGCCGAACAGTTATTGCCCCGCCGGTGTTTgcc > 2:75686/1‑249 (MQ=255) gATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACt > 1:430042/1‑251 (MQ=255) aCCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGCGTTTGACTTCgg > 2:344604/1‑251 (MQ=255) cTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCggg > 2:339398/1‑250 (MQ=255) tctcAAAAATGAGTGGCAACCCG‑ACATCCTGGCAGGGGAATGGCGAACCGCTGTTGACTCGCGACCCAAAGACCAACTCCTCCAAGCCGATGACCGACACCCACAAGCAGGAGTACGAAGGGCTGTGGCACCCGAATCATTTGCAGATCATGCAGGACCATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGAt < 2:373883/250‑1 (MQ=255) cAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATg < 1:1558/248‑1 (MQ=255) aaaaaaTGGGTGGCAAACCT‑ACAGCCTGGCGGGGGAATGGCGAACCGCTGTGGCCTCGGGAGCAAATGATCAACTTCTTCAAGCCGATGAGCGACACCCACAAGCAGGAGTACGAAGGGCTGTGGCATCCGAATCATTTGCAGGTCATGCAGGAACATCTGCGCGACTTGGCCGTAGAACAGAGGGATGGTTAATTGCTGAGCATCAGCCGCACATTTATTGCCCCGCCGGTGTTTGACTTCGGGATgcgc < 2:22343/250‑1 (MQ=255) tGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCAcc > 1:399781/1‑251 (MQ=255) aGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCt > 1:315491/1‑250 (MQ=255) aGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCt > 1:130591/1‑250 (MQ=255) tGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGAGTTTGACTTCGGGATGCGCTGCACCTAc > 2:160502/1‑250 (MQ=255) ggCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACAt > 2:152343/1‑251 (MQ=255) aacccG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGAATCGCGAGACAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCCCAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTg > 2:228642/4‑251 (MQ=255) aaCCG‑CCATCCTGGCCAGTGAATGGCGAATCGCTGTTGAATCGCGAGCCAAAGATCAACTTATACAAGCCGATGATCGACAACCACAAGCAGGCGTACGCAGGGCTGTGGCAACCGAATCATTTGAAGATCCTGCAGGAACATCAGCGCGACTTTGCAGTGGACCAGAGCGATGGTGAAGAGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGAGTTTGACTTCGGGATGCGCTGCACCTCCATCTgg > 2:464633/1‑251 (MQ=255) aaCCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGCCTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAATGCCCCGCCGGTGTTTGACTTCGGTATTCGCTGCACCGACATCTgg > 1:314221/1‑251 (MQ=255) aCCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGTGATGCGCTGCACCTACATCTGgc > 1:278958/1‑251 (MQ=255) ccG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCACCTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACCGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgcg > 2:106924/1‑251 (MQ=255) cG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGTGAGCCAAAGACCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGGGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGGGAAGTGCTGATCATCAGCCGCACAGTTATGGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCCCCTACATCTGgcgc < 1:222905/251‑1 (MQ=255) g‑ACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCg > 1:374347/1‑224 (MQ=255) aTCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCg > 1:114628/1‑251 (MQ=255) gagggggAATGGCGATTCGCTGTTGACTCCGGAGCCAAAGACCAACTTCTCCAAGCCGAGTACCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCGGATCATGCAGGAACATCTGCGCGACTTTGCCGTAAAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCg < 1:88285/245‑1 (MQ=255) gCAGGGGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTCCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCg < 1:438439/251‑1 (MQ=255) tGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAgg > 1:104169/1‑251 (MQ=255) acGCTGTTGACTCGCGAGCCAAAGATCACCTTCTTCAAGCCGATGACCAACACCCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGTAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGt < 1:133770/250‑1 (MQ=255) | ATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCG‑ACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGT > NC_000913/3224655‑3225139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |