Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,128	G→A	intergenic (‑52/‑366)	aer ← / → patA	aerotaxis receptor/putrescine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,128	0	G	A	100.0%	112.8 / NA	35	intergenic (‑52/‑366)	aer/patA	aerotaxis receptor/putrescine aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/22);  total (13/22)

TTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAG  >  NC_000913/3218889‑3219365                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              tttGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACGCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCGTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTGGCTGGGTGACATACTGATGAGAAGACATGTTCGCGTCCGGTGCAGGTCATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGc                                                                                                                                                                                                                                     <  2:76479/250‑1 (MQ=255)    ggCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCg                                                                                                                                                                                                                                 <  1:347721/251‑1 (MQ=255)           ccGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCt                                                                                                                                                                                                                          <  1:238795/251‑1 (MQ=255)                  aCGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCg                                                                                                                                                                                                                   <  1:246525/251‑1 (MQ=255)                            ttgtGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTg                                                                                                                                                                                                          >  1:236854/1‑250 (MQ=255)                                      gCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGCTCGc                                                                                                                                                                                               >  1:181476/1‑251 (MQ=255)                                              aaCTCTTGCAAGGTATAGCCGCTCACCTGCACAAAATGGTCATTAGCATGAGTGATATAGCGTTGCAGATCGGTAGTGGATATCAGAGGGGTATCGTCCGGCAGCGGGGTATTGTGCTGGGTGACATACGGATGAGAAGACATGTGCGCGTCCTGTGCAGGGTATAGGGGTGTTAACTGCTTGTCAGAGTTTATATCGGCCCCGGTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCg                                                                                                                                                                                       <  1:211761/251‑1 (MQ=255)                                                    tGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGAtt                                                                                                                                                                                    <  1:54111/248‑1 (MQ=255)                                                        aGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAacac                                                                                                                                                                             >  2:353827/1‑251 (MQ=255)                                                            ataGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAAGTTGCGATTTAAACACAAAt                                                                                                                                                                         <  1:367460/251‑1 (MQ=255)                                                                          gCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGAt                                                                                                                                                            <  2:342144/250‑1 (MQ=255)                                                                                 aGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCTGAGTGGTATCGTCCGCCAGCGTGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGATTTGATAGCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATa                                                                                                                                                    <  1:74017/251‑1 (MQ=255)                                                                                           gCATGAGAGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCAGACAGCGGGGTATTTTGCTGGGAGACATACGGATGCGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACAAAAATCTAATTCCTTGATTTAAAATACTTTCAcgct                                                                                                                                          >  2:279398/1‑248 (MQ=255)                                                                                            cATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATCCTTTCACTCTg                                                                                                                                         <  2:54740/251‑1 (MQ=255)                                                                                               gAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGtt                                                                                                                                       <  1:353827/250‑1 (MQ=255)                                                                                                           tttGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGCTTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCGGTTACTATACGAAAAc                                                                                                                          >  2:406043/1‑251 (MQ=255)                                                                                                              gCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTTCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGt                                                                                                                        <  2:205719/250‑1 (MQ=255)                                                                                                               cAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGtt                                                                                                                       >  2:255363/1‑250 (MQ=255)                                                                                                                   tcgggcgTGGACATCAGCGTGGTATCGTCCGCCAGCGGGGTCTTTTGCTGGGTGACATCCGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGAAAGAGTTTATATCGGCCCCGCTGCGGTTCTCTTTCACCGATTAATTTGTTTTAGATCGCAATTTGCGCTTTAAACACAAATCTACTTCCTTGTTTTAAAATACTTTCCCTCTGTTACTATACGAAAACGTTAAtt                                                                                                                   >  2:413964/7‑250 (MQ=255)                                                                                                                           ggACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGcc                                                                                                           <  2:54829/250‑1 (MQ=255)                                                                                                                                                           tATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTg                                                                          >  2:459655/1‑251 (MQ=255)                                                                                                                                                               ttGCTGGGTGACATACGGATGAGAAGACATGTGCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAAtgtggat                                                                       <  2:6348/250‑4 (MQ=255)                                                                                                                                                                   tGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGATGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGTTTTAGATCGCAATTTGCGATTTAAACCCAAATCTAATTCCTTGATTTAAAAAACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCACTGAAAAAGTGCATTTgcg                                                                  >  2:176403/1‑251 (MQ=255)                                                                                                                                                                    gggTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAAGTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTgcg                                                                  <  2:84452/250‑1 (MQ=255)                                                                                                                                                                               ggATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCgca                                                                <  1:3011/241‑1 (MQ=255)                                                                                                                                                                               ggATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCgca                                                                >  2:3011/1‑241 (MQ=255)                                                                                                                                                                                         aCATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAGTACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATAcc                                            <  1:163886/251‑1 (MQ=255)                                                                                                                                                                                          cATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATAccc                                           >  1:340722/1‑251 (MQ=255)                                                                                                                                                                                          cATGTGCGCGTCCTTTGCATGTTATATGGTTGTTATCTTCGTGTCAGAGTTTATATCGGCCCCGCTGCGTTTATCTTTAACCTATTAATTTGATTTAGAACGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTTCTATACGAAAACGGTAATTATCTTGCCCAAAAATCAGGCAATTAGGGCCCTGAAAACGTGCGTTTGCGCAACATTCATCCTATCCATatcc                                           <  2:312191/251‑4 (MQ=255)                                                                                                                                                                                                cgcgTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACTCAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACaaa                                     <  2:350862/251‑1 (MQ=255)                                                                                                                                                                                                     ccTGTGCAGGTTATTTGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCTCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATGGCCCTGAAAACGTGTAGTTGCGCAGCAATCATCAAATCCAGTCCCGACAAAAAcc                                 <  2:395156/250‑1 (MQ=255)                                                                                                                                                                                                         tgCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAGCACAAATCTAATTCCTTGATTTAAACTACTTTCACTCTGATACTATACGAAAACGATAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAACAcccgatc                            >  2:263279/1‑246 (MQ=255)                                                                                                                                                                                                         tgCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCAGCCCCGCTGCGGTTATCTTTAAACGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAACTCCTTGATTTAAAATACTTTCACGCTGTTACTATACGAAAACGTTAATTACCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATTCCCGACAAAAAcc                                 <  1:263279/246‑1 (MQ=255)                                                                                                                                                                                                                           tAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGtt           >  1:58973/1‑250 (MQ=255)                                                                                                                                                                                                                                    gTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAg  <  1:286524/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              TTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAG  >  NC_000913/3218889‑3219365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑