Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,193,351	G→A	G163E (GGG→GAG)	yqiK →	flotillin family inner membrane protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,193,351	0	G	A	100.0%	187.6 / NA	59	G163E (GGG→GAG)	yqiK	flotillin family inner membrane protein YqiK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/24);  total (35/24)

ATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGG  >  NC_000913/3193113‑3193593                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   aTTGATAACCTAATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGACCAGACGCTGGGGCAACGCCCCCTGTCGCCTGAAGACTTACGTATGGTGGTTGAAGATAAATTTGTCGAGGCCCTCCGTGCAACAGCTGAGCAAATGACCATGCCTGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATAc                                                                                                                                                                                                                                        >  2:308002/1‑251 (MQ=255)         ccTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGc                                                                                                                                                                                                                                  >  1:3135/1‑249 (MQ=255)                aCGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCt                                                                                                                                                                                                                         <  2:254931/251‑1 (MQ=255)                 cGAAAGAGCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTg                                                                                                                                                                                                                        <  1:283033/251‑1 (MQ=255)                          gTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGGGCAAAATACAGTGGCAGAAGACCTGTCGAAAAAc                                                                                                                                                                                                               >  1:262080/1‑251 (MQ=255)                           tATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACg                                                                                                                                                                                                              >  1:384380/1‑251 (MQ=255)                                 cgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTgg                                                                                                                                                                                                        >  2:373551/1‑251 (MQ=255)                                    cGATGTAGTAGTCGCTTTCTGTGTGCGGGTAAAACCTTCAGTAGAAGGTATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGAGTTACTTATGTTGGTTGAAGATAAATGTGTCGATGCCCTCCGTGCAACAGCTGCTCAAATGACCATGCATTAGTTACAGGATACGCGCTAGAACTTTGTGCATGAGGTGCAAAATACAGTGCCAGAAGACCTTTCGAAAAACGGTCTGGaa                                                                                                                                                                                                      <  2:438069/250‑1 (MQ=255)                                     gatttagtagTCGGTTTTTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAATTACAGTGTCAGAAGACCTTTCGAAAAACGGTCTGGAACt                                                                                                                                                                                                    <  2:353202/247‑1 (MQ=255)                                           gtagtaGCTTTCTTTGTGAACGTCAAAAATTCAGTCGACGGGATCGCCACCGCTGCCCAGACGATGGGGCAACGCACCCTGTCGACTGCAGCCTTAAGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGCGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGCTACCCGCGAGAACTTTGTGCAGGAGGTGCACAATACAGTGGCAGAAGACATGTCGTAAAAAGGTCTGGAACTGgag                                                                                                                                                                                                >  2:48871/1‑249 (MQ=255)                                                 gCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCCCCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGCTGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTc                                                                                                                                                                                        >  1:303852/1‑251 (MQ=255)                                                      ctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACtt                                                                                                                                                                                    >  1:68961/1‑250 (MQ=255)                                                         tgtgCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTGTCACTTAcc                                                                                                                                                                                 <  2:68961/250‑1 (MQ=255)                                                             cGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACtt                                                                                                                                                                            <  2:384380/251‑1 (MQ=255)                                                              gggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACttt                                                                                                                                                                           <  2:315472/251‑1 (MQ=255)                                                               ggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTa                                                                                                                                                                          <  1:81137/251‑1 (MQ=255)                                                                    aaCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAAcc                                                                                                                                                                       >  2:376145/1‑249 (MQ=255)                                                                     aCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAAcc                                                                                                                                                                       >  1:216850/1‑248 (MQ=255)                                                                        ttCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAg                                                                                                                                                                     >  1:415486/1‑247 (MQ=255)                                                                        ttCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCt                                                                                                                                                                 >  1:375981/1‑251 (MQ=255)                                                                        ttCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAGTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTGACCAACTTTAACCAg                                                                                                                                                                     <  2:415486/247‑1 (MQ=255)                                                                                aaGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTGAACCAGACCTCGAAAGaa                                                                                                                                                         <  2:303852/251‑1 (MQ=255)                                                                                       tGCCACCGCTGACCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGCCTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTc                                                                                                                                                   >  2:57840/1‑250 (MQ=255)                                                                                           aCCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGACCTTTGTGCAGGAGGGGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTAACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATcc                                                                                                                                              >  2:335011/1‑251 (MQ=255)                                                                                                    cAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGc                                                                                                                                     >  1:212814/1‑251 (MQ=255)                                                                                                    cAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGc                                                                                                                                     <  1:342015/251‑1 (MQ=255)                                                                                                      gACGCTGGGGAAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCt                                                                                                                                   >  1:111296/1‑251 (MQ=255)                                                                                                      gACGCTCGGGCAACGCCCCCTGTCGCCTGACGACTTCCGTCTGTTGGTTGCAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGACACACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCACTCCGAACAATGCCt                                                                                                                                   >  2:41132/1‑251 (MQ=255)                                                                                                                 aaCGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGAGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGAAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGAAGCCGa                                                                                                                         >  1:201067/1‑250 (MQ=255)                                                                                                                         cTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGGGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAAc                                                                                                                >  2:4223/1‑251 (MQ=255)                                                                                                                                  gAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGa                                                                                                        <  1:316810/250‑1 (MQ=255)                                                                                                                                            gTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACa                                                                                             <  1:373551/251‑1 (MQ=255)                                                                                                                                              aTGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACaga                                                                                           <  1:204200/251‑1 (MQ=255)                                                                                                                                              aTGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGGTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACaga                                                                                           <  1:22439/251‑1 (MQ=255)                                                                                                                                                gttggttgAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAgc                                                                                         <  1:376145/251‑1 (MQ=255)                                                                                                                                                    gttgAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTAAAGGACACCCGCGAGAACTTTGTGCGGGAGGTGCAAAATACAGTCGCAGCAGCCCTGTCGAACAACGGTCTGGAACTGGAGAGCGTTTCACTTACCACCTTTAACCAGAGCTCGCAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAACCTGACTCAGGAACCAGAGCGCCg                                                                                     >  1:461092/1‑251 (MQ=255)                                                                                                                                                             aaaTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGa                                                                            <  1:122948/251‑1 (MQ=255)                                                                                                                                                              aaTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACAAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGa                                                                            >  2:145232/1‑250 (MQ=255)                                                                                                                                                               aTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGCAGACCTGTCGAAAAACGGGCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTAGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTCATCAAACTGACTCAGGAAACAGAACGCCGTCGCCGCGAAc                                                                          >  1:46773/1‑251 (MQ=255)                                                                                                                                                                      gATGCACTCCGTGCAACAGATGCGCAAATGACAATGCATGAGGTACAGGATACCCGCGCGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAATGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACg                                                                    >  2:172771/1‑250 (MQ=255)                                                                                                                                                                         gCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGt                                                                <  1:150734/251‑1 (MQ=255)                                                                                                                                                                              acGTGCAACAGCTGCGCAATTGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACATGTCGAAAAACGGGCTGGAACTGGAGAGCGTTTCACTAACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGCGCGCCGTCGCCGCGAACGTAACGAAGTTGAAc                                                           >  2:66028/2‑251 (MQ=255)                                                                                                                                                                               cGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGAGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGCACGTAACGAAGTTGAAc                                                           >  1:55794/1‑250 (MQ=255)                                                                                                                                                                                    aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTCCCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAACCTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATg                                                     >  1:381400/1‑251 (MQ=255)                                                                                                                                                                                    aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATg                                                     >  2:107045/1‑251 (MQ=255)                                                                                                                                                                                        gCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAATGAAGTTGAACAGGATGTAGa                                                 <  1:326154/251‑1 (MQ=255)                                                                                                                                                                                           gcgcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTTGAAGt                                              <  2:433711/251‑1 (MQ=255)                                                                                                                                                                                             gcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCAGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTg                                            >  1:2186/1‑251 (MQ=255)                                                                                                                                                                                                         gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaa                                 >  2:12530/1‑250 (MQ=255)                                                                                                                                                                                                         gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaa                                 >  1:303049/1‑250 (MQ=255)                                                                                                                                                                                                                ttACAGGATACCCGCGAGAACTTTGTGCCGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTAAACAGGATGTAAAAGTTGCGGTGCGTGAAAAAAATcg                         >  2:389286/1‑251 (MQ=255)                                                                                                                                                                                                                    aGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATTTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAt                     <  1:402631/251‑1 (MQ=255)                                                                                                                                                                                                                    aGGATACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAt                     <  1:107045/251‑1 (MQ=255)                                                                                                                                                                                                                    aGGATACCCGCCAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGTAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAt                     <  2:329832/251‑1 (MQ=255)                                                                                                                                                                                                                       aTACCCGCGAGAACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGCACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATgcg                  >  2:192494/1‑251 (MQ=255)                                                                                                                                                                                                                            cgcgAGAACGTTGTGCAGGAGGTGAAAAATATAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACGGGAGAGCGTTTCACTCACCAACTTTAAGCAGACCTAGAAAGAACAGTGCAATCCGAAGAATGCGTTGGACGGAGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGGTGAGCAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGAGGCGCTATc             <  1:151794/251‑1 (MQ=255)                                                                                                                                                                                                                                 gaACTTTGTGCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCgcgc         >  1:214557/1‑250 (MQ=255)                                                                                                                                                                                                                                       tgtgCAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTgg  >  1:215348/1‑251 (MQ=255)                                                                                                                                                                                                                                           cAGGAGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGCAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTgg  >  2:256615/1‑247 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   ATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAAAAAAATCGCGATGCGCTATCGCGCAAGCTGG  >  NC_000913/3193113‑3193593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑