Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,145,944	G→A	L106L (CTC→CTT)	hybO ←	hydrogenase 2 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,145,944	0	G	A	100.0%	176.9 / NA	51	L106L (CTC→CTT)	hybO	hydrogenase 2 small subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/36);  total (15/36)

ATATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGG  >  NC_000913/3145699‑3146182                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               atatTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCGTTGTACTTCACAAGAGc                                                                                                                                                                                                                                           <  2:12646/251‑1 (MQ=255)   atTGATAACGGTTTTGCCGGGCAGAACTTCTTGCAGGCGGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCGTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCg                                                                                                                                                                                                                                          <  2:349257/250‑1 (MQ=255)     tGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCGTTGTACTTCTCAAGAGCGtt                                                                                                                                                                                                                                        <  1:113544/250‑1 (MQ=255)        tAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTAtgt                                                                                                                                                                                                                                    >  2:138453/1‑251 (MQ=255)                ttGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTtct                                                                                                                                                                                                                             <  1:215978/250‑1 (MQ=255)                   ccTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTc                                                                                                                                                                                                                         <  1:127697/251‑1 (MQ=255)                         aGAACTTCTTGGAGGTGGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAATCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCTTCCTCCACGAACACATACTTTTCTTTGGACTTTTCAAGAGCTTTATGTTTGTTCTCTTCGACCtg                                                                                                                                                                                                                   <  2:462420/251‑1 (MQ=255)                          gAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCtga                                                                                                                                                                                                                  <  1:268040/251‑1 (MQ=255)                                attGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGAcc                                                                                                                                                                                                             <  2:183409/249‑1 (MQ=255)                                   gCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAg                                                                                                                                                                                                         <  1:73264/251‑1 (MQ=255)                                   gCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAg                                                                                                                                                                                                         <  1:127624/251‑1 (MQ=255)                                       ccTGACTGCGCCAGGTGGGTTAACTCCAGCTGCGGCAACCCCGCCCCACGCATAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTGGCGGATGTGATCCACAATCGGCTCACCGGCAACCAGGCAATAAAGACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTGCTCAAGAGCGTGATGTTTGTTCGCGGCGACCTGATGACCGGAggcgg                                                                                                                                                                                                     <  2:291719/250‑1 (MQ=255)                                          gACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcggc                                                                                                                                                                                                    <  1:335592/249‑1 (MQ=255)                                                      tGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCg                                                                                                                                                                                       <  1:130843/250‑1 (MQ=255)                                                      ggggTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGTAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTGGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCg                                                                                                                                                                                       <  2:385591/249‑1 (MQ=255)                                                       gggTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGt                                                                                                                                                                                      <  1:249816/250‑1 (MQ=255)                                                            aaCTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCTCCTCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCTGCGGAAAGCACTTCGTGATAc                                                                                                                                                                                 <  1:164306/250‑1 (MQ=255)                                                            aaCTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATAc                                                                                                                                                                                 <  1:48194/250‑1 (MQ=255)                                                                  aGCTGCGGCATCACCGCACCACGCAGAGAAGGAACCGAGAGCGATAATGGCTGCTGCGCCTTCCGCCGATTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTGAATGGGATGGAACCATCCACCACTAACACATACTGCCCTGTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGTAAAGCACTGCGTGAGACTTCagag                                                                                                                                                                          <  2:277138/251‑1 (MQ=255)                                                                   gCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCagaga                                                                                                                                                                         <  1:376740/251‑1 (MQ=255)                                                                        ggCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGAt                                                                                                                                                                      >  2:349764/1‑249 (MQ=255)                                                                                cGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGACTCCAGt                                                                                                                                                            >  1:369495/1‑251 (MQ=255)                                                                                  ccccACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATTGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTAc                                                                                                                                                          <  1:240109/251‑1 (MQ=255)                                                                                  ccccACGCAGAGCAGGAACCGAAAGCGATAATGGCTGCTGCGCCTGCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAATGCTGCGGAAAGCACTGCGTGATACTCCAGAGAGATAGTCTCCAGTAc                                                                                                                                                          <  2:268742/251‑1 (MQ=255)                                                                                               aGGAACCGATAGCGATAATGGCTGCTGCGCCGTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGATGTGTTCTAcc                                                                                                                                             <  2:312684/251‑1 (MQ=255)                                                                                                      gATAGCGCTAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGCTGTGATCCACAATCGGCTCACCGGCCACCCTGCCATAAATACCGTTATCTTTTAATGGGATGGCACCATACACCCCTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAATCTCCAGTACGAGGTTTTCTAACGTTGGAt                                                                                                                                      >  2:175698/1‑251 (MQ=255)                                                                                                             aTAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGc                                                                                                                               <  1:120525/251‑1 (MQ=255)                                                                                                                aTGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACg                                                                                                                            <  1:202367/251‑1 (MQ=255)                                                                                                                aTGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTAGCTTTTAATGGGATGGAACCATCCACCACTAACACATTCTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACg                                                                                                                            <  1:279998/251‑1 (MQ=255)                                                                                                                        tgcGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCag                                                                                                                      >  2:455436/1‑249 (MQ=255)                                                                                                                            ccTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTc                                                                                                                <  2:105334/251‑1 (MQ=255)                                                                                                                                                gtgATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTcc                                                                                             <  2:72665/250‑1 (MQ=255)                                                                                                                                                 tgATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCACCCCCCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGATTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGCGCTACTCCAGAGAGATAGACTCCAGAACGAGGTTTTCTACCGTTGGATGCGTTGCCCGAAGAAGAGTTTCCGTACAACCGGTGCACtactc                                                                                           >  1:304359/1‑247 (MQ=255)                                                                                                                                                   aTCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTgcg                                                                                         <  2:333225/251‑1 (MQ=255)                                                                                                                                                        caATCGGCTCACCGGCAACCCTGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGcc                                                                                     >  2:435186/1‑250 (MQ=255)                                                                                                                                                         aaTCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCaa                                                                                   <  1:322654/251‑1 (MQ=255)                                                                                                                                                                    cGGCAACCATGCAATAAATACCGTTATCTGTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTGTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAAca                                                                        <  2:267718/251‑2 (MQ=255)                                                                                                                                                                     ggCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCGTTGTACTGCTCAAGAGCGTTATGTTTGTTCTCTGCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACGCCAGAGAGATAGTCTCCATTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAGGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTg                                                                       <  1:73447/251‑1 (MQ=255)                                                                                                                                                                                aataaataCCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGc                                                            <  1:435186/251‑1 (MQ=255)                                                                                                                                                                                 ataaataCCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCg                                                           >  1:408107/1‑251 (MQ=255)                                                                                                                                                                                        ccGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGt                                                    >  1:21323/1‑251 (MQ=255)                                                                                                                                                                                             aTCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGATTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCg                                               >  2:86348/1‑251 (MQ=255)                                                                                                                                                                                                  ttAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGAGAACTGGCGGACGCTGCGGGTTAGTAACCGATTc                                           <  1:393404/250‑1 (MQ=255)                                                                                                                                                                                                      tGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGcc                                       >  1:250611/1‑250 (MQ=255)                                                                                                                                                                                                          aTGGAACCATCCACCACAAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGAACGCGGCGGGTTAGTAACCGATGCGGCCAtctc                                  >  2:487/1‑251 (MQ=255)                                                                                                                                                                                                                    ccaccaCTAACACATACTGCCCTTTGTTCTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTTCGAGTTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATACCTGGCGGACGCTGCGGGGTAGTAACCGATTCGGCCATCTCTGCAGCGGCt                        <  2:811/251‑1 (MQ=255)                                                                                                                                                                                                                              cacaTACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCAATTCGTGATACTCCAGAGAGATAGTCTCCCGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGACGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGCCGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACtt               >  1:197132/1‑250 (MQ=255)                                                                                                                                                                                                                                    cTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAAcccc         >  2:50447/1‑250 (MQ=255)                                                                                                                                                                                                                                      gCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCAt       <  2:154456/250‑1 (MQ=255)                                                                                                                                                                                                                                          tttGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCAtggtgg  >  1:465471/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCAtggtgg  <  1:349764/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ATATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGG  >  NC_000913/3145699‑3146182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑