Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,263	G→A	A45T (GCC→ACC)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,263	0	G	A	100.0%	73.8 / NA	23	A45T (GCC→ACC)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

TCAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTAC  >  NC_000913/3134018‑3134502                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                tCAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTCACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCgg                                                                                                                                                                                                                                             >  1:219031/1‑250 (MQ=255)              gACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGt                                                                                                                                                                                                                                <  1:460939/250‑1 (MQ=255)                                              aCGCTTTCATAAAAACATATTAACCAAATAAATATTGTTAATGGATATTGAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGACGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTTCCGTTGATGGTAAAATc                                                                                                                                                                                                <  2:284142/250‑1 (MQ=255)                                                        aaacaCATATTAACAAAATAAATATTTTTAATGGATCTTTAAATTAAAGGATATATTAATGCAGTCAATAACACCACCATTAATTGCCATAATTGGGAGCGATGGTTCAAGAAAGACAACGGTGTGTGAACATCATATTACCGTTTGCGAAAATTATGATGCTGCCGAAAGAGTTCATTTAGGCAAACGGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATaaa                                                                                                                                                                                      >  2:263173/5‑250 (MQ=255)                                                                atTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACAACTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGACAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGATCTTTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTAATGGGCAAATCCTTACATAAAACAATTGa                                                                                                                                                                              >  2:244761/1‑250 (MQ=255)                                                                 ttAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGACAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAAc                                                                                                                                                                            >  2:419225/1‑251 (MQ=255)                                                                           aaataTTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGt                                                                                                                                                                  <  2:23614/251‑1 (MQ=255)                                                                                 ttttAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAAc                                                                                                                                                            >  1:463244/1‑251 (MQ=255)                                                                                           tatTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCaaaaaa                                                                                                                                                   >  1:90014/1‑250 (MQ=255)                                                                                            atTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAAt                                                                                                                                                 >  1:98273/1‑251 (MQ=255)                                                                                               taaattaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTg                                                                                                                                               <  1:134883/250‑1 (MQ=255)                                                                                                  attaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATGGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACTGCAAAAAAATTGcttg                                                                                                                                           <  2:94417/251‑4 (MQ=255)                                                                                                     aaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGAc                                                                                                                                        <  1:401495/251‑1 (MQ=255)                                                                                                      aaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGaaaa                                                                                                                                                             >  2:377573/1‑229 (MQ=255)                                                                                                      aaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAAGTGAACGAAATCAGGTGaaaa                                                                                                                                                             <  1:377573/229‑1 (MQ=255)                                                                                                         gATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGt                                                                                                                                    <  1:319115/251‑1 (MQ=255)                                                                                                          atatatTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGAGACAAAATTACCGTTGATGGGACAATCCTTACATAAAACAATTGAACGTAATCAGGTGAAAACAGCACAAAAATTGCCTGGACCAGtt                                                                                                                                   >  1:83216/1‑251 (MQ=255)                                                                                                               ttCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAACTGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGgc                                                                                                                              >  1:209161/1‑251 (MQ=255)                                                                                                                                                      ggTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTgcg                                                                                       >  2:142581/1‑251 (MQ=255)                                                                                                                                                                                        aaCATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGTTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACAAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTCATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCggg                                                     >  2:100680/1‑251 (MQ=255)                                                                                                                                                                                                         gTCGAAAAATATGGTGCTGCCGAAAGAGTTCAGTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAAGCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCGTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGGTCTAgccg                                     <  2:285758/250‑1 (MQ=255)                                                                                                                                                                                                                                 agagTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGGGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTcc            <  2:8805/251‑1 (MQ=255)                                                                                                                                                                                                                                            aGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAAATACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGTTGAAAACAGCAAAAAAATTGCCTGGACCAGTGCCGGCGCTGGTAAGTACAGCGTTTGTCGTCCGTCGTTTACTGCGCTTTCGTCATATGCTTTCCTGTCTCCGTCGCGGGTGAATTGTTCTAACCGACCGTTAGCCTCATGACCAAATTCCTGGCGCTTAc  <  2:450192/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TCAACCTTATTGTGACAATGGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTAC  >  NC_000913/3134018‑3134502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑