Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,121,033 T→C I83V (ATT→GTT)  glcA ← glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,121,0330TC96.7% 92.4 / NA 30I83V (ATT→GTT) glcAglycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (15/14);  minor base A (1/0);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

AATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGG  >  NC_000913/3120794‑3121272
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               
acgcacacacCGCCCCGCCGAGTATACCGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACGGGCGCACCAAAGCCAGCCGCCCCTTCCAGCACCGCACCAAAGGAGAAACCAACCAGTAACACCTGCAAACGCTGATCGGCGGTGAGGGAGATAACCGAGCTG‑CGGATAATACCAAACGGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTg                                                                                                                                                                                                                                      <  2:116530/245‑1 (MQ=255)
  tCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGc                                                                                                                                                                                                                                    >  1:347091/1‑251 (MQ=255)
      acacAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCCAGCTATTGGCCAt                                                                                                                                                                                                                                 >  2:402771/1‑250 (MQ=255)
         caGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATc                                                                                                                                                                                                                             >  2:138954/1‑251 (MQ=255)
          aGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATcc                                                                                                                                                                                                                            >  2:110550/1‑251 (MQ=255)
                 ccgcGTAGAACGGTTTGAAGCCCAGGCCCACCAGCACCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGCAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTCCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCataaata                                                                                                                                                                                                                     <  1:402771/251‑1 (MQ=255)
                     gTATACCGGTTAGACGCCCAGGCCCCCCAGCAGCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGAAACGCCCCAAAGGAGAACCAAATCAGTACCCCCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATACCGACCTGCCCGCGGGCAACGGTTAATTTATACAGAAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGc                                                                                                                                                                                                                 <  2:395117/251‑1 (MQ=255)
                                 gAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCACAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCgc                                                                                                                                                                                                       >  1:150939/1‑249 (MQ=255)
                                                                          gcgcACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATAtt                                                                                                                                                            <  1:69181/251‑1 (MQ=255)
                                                                                               cTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAatat                                                                                                                                        >  1:247500/1‑250 (MQ=255)
                                                                                                 tCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAAGATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATaa                                                                                                                                      <  1:390796/250‑1 (MQ=255)
                                                                                                        aCGCACCAAAGGAGAAACCAAACAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTAtt                                                                                                                              <  1:110550/251‑1 (MQ=255)
                                                                                                              cAAAGGAGAAACAAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCa                                                                                                                        <  1:116469/251‑1 (MQ=255)
                                                                                                                          caagcagaaaCACCGGCAAGCGCTGATCGGCGGTGATGGAGATAACCGA‑CTGTCGGATAATATCGACCTGCCCGTTGGCAACGGTTAATTTATACAGAAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAAGGCCATATCAATCGGCATTTTAAAGGCGAATAGTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCt                                                                                                            <  1:231187/243‑1 (MQ=255)
                                                                                                                              cAGTAACACCTGAAAACGCTGACCGTCGGTGAGGGAGATACCCGAGCGG‑CGTATAAAATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGAAACACCGCCGCGACAAAAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAAACGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCa                                                                                                        <  1:72733/251‑1 (MQ=255)
                                                                                                                                    cacCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCCTAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCCGCGACATGTCCTTTCAGACGt                                                                                                   >  1:275279/1‑250 (MQ=255)
                                                                                                                                                 tGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGCCGTAATACCGCGAGTGc                                                                                     >  2:5919/1‑251 (MQ=255)
                                                                                                                                                 tGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGc                                                                                     >  1:103794/1‑251 (MQ=255)
                                                                                                                                                         ggTGATGGAGATAACCGGGCTG‑CGGATCATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGAGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAACGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGATCCAGCGACATGTCCTTTCAGACGTAATACCGCGTGTGCAACga                                                                                >  2:464221/1‑248 (MQ=255)
                                                                                                                                                         ggTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATAACGCGAGTGCAACga                                                                                >  2:232215/1‑248 (MQ=255)
                                                                                                                                                                              g‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAgg                                                         <  1:138954/250‑1 (MQ=255)
                                                                                                                                                                                   ataataTCGAACTGCCCGCGGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAAAAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTAAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATAAGGGCGAc                                                    >  2:397059/1‑251 (MQ=255)
                                                                                                                                                                                                      tGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCACGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGt                                 >  1:445098/1‑251 (MQ=255)
                                                                                                                                                                                                      gggaaaCGGTTAATTTATACAGGACCACCGCCGCGACAATACCCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTGCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCTCAGt                                 <  2:345854/247‑1 (MQ=255)
                                                                                                                                                                                                          aaCGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCACTCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGtcatc                             >  1:106325/1‑248 (MQ=255)
                                                                                                                                                                                                                      ataCAGGAACACCGCCGCGACAATAAACCACGCTATTGGCCATAATCCCTAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATACATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGCTAATATAAGGGTTATTGCTCCAGCGCCATGTCCTTTCAGACGTCATACCGCGAGTGCAACGAAGAAGAATATTCTCGGGATCAGGTCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCatat                 >  2:256039/1‑251 (MQ=255)
                                                                                                                                                                                                                            gAACCCCGCCGCGACAATAACCCACGCGATTGGCTATAATCCATAAATAAAGCCATAGCCCGCGGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGTTTATTGCTCCAGCGACATGTTCTGTCAGACGTAATTCCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCACCCATCGGCATATACAttt           <  1:55346/251‑1 (MQ=255)
                                                                                                                                                                                                                             aaccccgccgCGCCAATACCCCACGCTATTGGCCATATTCCATAAATAAAGCCATAGCACGCATCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTg          <  2:2497/247‑1 (MQ=255)
                                                                                                                                                                                                                                    ccgcGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATCTAAGGGTTATTGCTCCGGCGACATGTCATTTCAGACGTAATACCGCGAGTGAAACGACGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGACTATACATTTGGGTcc     >  1:228923/1‑249 (MQ=255)
                                                                                                                                                                                                                                     cgcgACAATAACCCACGCTATTGGCCATAATACATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATAATGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATAGACATTTGGGTCCAgg  <  2:257504/251‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               
AATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTG‑CGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAGG  >  NC_000913/3120794‑3121272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑