Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,260	T→C	V326A (GTG→GCG)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,260	0	T	C	98.6%	222.2 / NA	69	V326A (GTG→GCG)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (29/39);  minor base A (1/0);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGA  >  NC_000913/3089013‑3089502                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gagaACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGAt                                                                                                                                                                                                                                                 <  1:62979/251‑1 (MQ=255)       aGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGgctgc                                                                                                                                                                                                                                           <  1:436547/251‑1 (MQ=255)              tCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCa                                                                                                                                                                                                                                      <  2:198947/249‑1 (MQ=255)               ccgccgCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGTGTTAT‑ACCAACCCTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGGTGACCGCTGGGG‑ACTTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTTGCATg                                                                                                                                                                                                                                    <  2:280900/250‑1 (MQ=255)                 cccgcGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGc                                                                                                                                                                                                                                 <  2:377930/250‑1 (MQ=255)                    gcgcgGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTa                                                                                                                                                                                                                              <  1:134035/251‑1 (MQ=255)                        ggTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTAATTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCAAGGGCGGACTCg                                                                                                                                                                                                                          >  1:352246/1‑251 (MQ=255)                         gtggtCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCgg                                                                                                                                                                                                                         <  1:460738/247‑1 (MQ=255)                             tCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCGGGCATGGGCGTACTCGGTAc                                                                                                                                                                                                                      <  2:220192/250‑1 (MQ=255)                              ccttgcagTACTGTTGCAGGTAATACAGCAATAACCCGGGTTGACCG‑TCATCATGTATTACGCGCCGAACCTCTACGACCTGGCGGGTTCT‑AACCACACTACCGAGCAAATGAGGGGGCCCGTGATTGTAGGCCTGACAAACGTAATTGCCACCTTTATCGCAATCGGCCTTGTTGACAGGTGGGG‑ACCAAAACCCAAGCTAACGCTGGGCTTCCTGGAGATGGATGCTGGCCGGGGCGTCCTCGGTACa                                                                                                                                                                                                                     >  2:221879/8‑250 (MQ=255)                                 tGGCGTTCTGTTGCCAGTAATGCAGCAAGTCCCCGGGATGAACGGCCACCA‑GTATTACGGCCCGAAAATCTTCGAACCGGCCGGTTATTACAACCACT‑CCGAGGCAATGGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAat                                                                                                                                                                                                                   <  2:170082/249‑1 (MQ=255)                                 tGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAat                                                                                                                                                                                                                   <  2:373688/249‑1 (MQ=255)                                              cAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTAt                                                                                                                                                                                                    <  1:204264/251‑1 (MQ=255)                                                   aaTGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCAct                                                                                                                                                                                               >  1:105617/1‑251 (MQ=255)                                                        agcaATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAACTCTTCGAACTGCCGGGTTAT‑ACCAACACTTACGAGCACAAGTGGGGGGCCCGGATTTACGGCCTGACCAACGTACTTGCCAACTTTTTCGCAATCGGCCTTGTTGACCGCATGGG‑ACGTAAACCCACGCTACCCCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGCACAATGATGCCTATCGGTTGTCACTATCCg                                                                                                                                                                                          >  2:92451/1‑251 (MQ=255)                                                        agcaAATCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAAGCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGTCCTGACCAGCGTACTTGCCAGCTTTATCGCAAGCGGCCTTGGTGACCG‑TGGGGTACGGAGGCCAACGCTAATGCTGGGCTTACTGGCGGTGGCTGCTGGTATGGGCGTAGTCGTTACAATGATGCATAGCggggttggcactccg                                                                                                                                                                                          <  2:232425/251‑14 (MQ=255)                                                               cACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACAATACCGAGCAAATGTGGGGGGCCGTGATTGTCGGACTGACCAAAGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTATCCGTCGgcgc                                                                                                                                                                                   >  2:41911/1‑251 (MQ=255)                                                                aCCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTAACGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTAGGTACAATGATGCATATCGGTATTCACTCCCCGTCGgcgc                                                                                                                                                                                   >  2:134525/1‑250 (MQ=255)                                                                   gggATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCAACTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCCTGGTCGTACTCGGGACAATGCTGCATATCGGTATTCACTCTCCGTCGgcgccgt                                                                                                                                                                                >  1:448817/1‑247 (MQ=255)                                                                     gATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAAAACTACCGAGCAAATGGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCCGCCTTGTTGACCGCTGGGG‑ACGTAAACCCACGCTAACGCTGGGCTTCCTGGCGAGGGCTGCTGGCATGGGCGAACTCGGAACAATGAAGCATATTGGTATTCACTCTCCGTCGGCGCAGCAtt                                                                                                                                                                             >  1:197463/1‑251 (MQ=255)                                                                      aTGAACG‑ACATCATGTATTACGCGCCGAAAATCTTAGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGCCCAACGTAATTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCAGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCAAGGGCGTACTCGGGACCATGATGAATATCGGAATTCACTCTCCGTCGGCGCAGGAttt                                                                                                                                                                            >  1:94575/1‑251 (MQ=255)                                                                       tGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTAc                                                                                                                                                                                                                      <  1:364657/208‑1 (MQ=255)                                                                       tGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTAc                                                                                                                                                                                                                      >  2:364657/1‑208 (MQ=255)                                                                                  catGTATTACGCGCCGACAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCCTATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgccatcgcc                                                                                                                                                                  >  1:383561/1‑250 (MQ=255)                                                                                  catGTATTACGCGCCGAAAATCTTCTAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCGCCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgccatcgcc                                                                                                                                                                  <  1:222351/250‑1 (MQ=255)                                                                                  catGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgccatcgcc                                                                                                                                                                  <  1:422198/250‑1 (MQ=255)                                                                                         tACGCGCCGAAAATCTTCGAACCGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATCTCGGTATTCTCTCTCCGTCGGCGCAGTATTTCGCCATCGCCAtgctgc                                                                                                                                                           <  2:226097/250‑1 (MQ=255)                                                                                                cgacaaTCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATAGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGAACTCGGGACAatg                                                                                                                                                                                                                  >  2:333136/5‑188 (MQ=255)                                                                                                cGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAatg                                                                                                                                                                                                                  <  1:333136/188‑1 (MQ=255)                                                                                                 gAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCCGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTTGCATGGGCGTACTCGGGACAATGATGCTTATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGtttt                                                                                                                                                  >  2:155161/1‑250 (MQ=255)                                                                                                     agcgtCGAACTGGGGGTTTAT‑ACCAACACTACCGTGCAAATGTGGGGGACCGTGATTGTCGCCCTGACCAACTTACTTGTCACCTTTATCGCAATCGGCCTTGTTGACCTCTGTGG‑ACGTAAACCAACGCTAACGCTTGTCTTCTTGTCGAGGTCTTCTTGCATGGGCGTACTCGTTACGATGAGGCTTATCTGTATTCTCGCTCCGTCGGCGCATTATTTCGCCATCGCCATGCTGCTGATGTTTATTGt                                                                                                                                              <  2:198584/247‑1 (MQ=255)                                                                                                     aTCTTCGAACTGGCGGGCTAT‑ACCTACACTACCGAGCACATGTGGGGGACCGGGCTTGTCGGCCTGACCAAAGTACTTGCCACCTTTATCGCAATCGTCCTTGTTGACCGCAGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACACTGATGCATATCGGTATTCACTCTCCGTCGGCCCAGTATTTCGCCATCGCCATGATGCCGATGTTTATTGt                                                                                                                                              >  2:12567/1‑251 (MQ=255)                                                                                                       cTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGCTGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGGATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGTTGTTTTTTGTCg                                                                                                                                            >  2:118286/1‑251 (MQ=255)                                                                                                         tCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGTCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATTCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCg                                                                                                                                            <  1:411298/249‑1 (MQ=255)                                                                                                         tCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTc                                                                                                                                             <  1:73383/248‑1 (MQ=255)                                                                                                           gAACTGGCGGGGTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTAGTTCGCCATCGCCATGCTGCTGATGTTTATTTTCGGttt                                                                                                                                        <  2:43541/251‑1 (MQ=255)                                                                                                               tGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGcc                                                                                                                                    >  1:310470/1‑251 (MQ=255)                                                                                                                ggCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTTATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGTCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCTCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCa                                                                                                                                   <  2:380475/251‑1 (MQ=255)                                                                                                                ggCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCCCCTTTATCGCAATCGGCCGTGTTGACTGCTGGGT‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCAGGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCTCTGTATTGCGGCATCGCCACGCGGCTGATGTTTATTGTCGGTTTTGcc                                                                                                                                    <  2:438057/250‑1 (MQ=255)                                                                                                                         t‑aCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCg                                                                                                                          >  2:359745/1‑251 (MQ=255)                                                                                                                           aCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTAGCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCgg                                                                                                                         <  2:25940/251‑1 (MQ=255)                                                                                                                                    aCCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGa                                                                                                                 <  1:118286/250‑1 (MQ=255)                                                                                                                                       gAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTg                                                                                                             <  1:164083/251‑1 (MQ=255)                                                                                                                                         gCAAATGTGGGGGACCGTGCTTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGCTTTggg                                                                                                           >  2:383551/1‑251 (MQ=255)                                                                                                                                         gCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGGTGACCGCTGGGG‑ACGTAAACCAACGCTAACGATGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTggg                                                                                                           >  2:96636/1‑251 (MQ=255)                                                                                                                                                        cGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGaaa                                                                                            <  2:310470/251‑1 (MQ=255)                                                                                                                                                               gTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCTGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGcc                                                                                     <  1:336438/251‑1 (MQ=255)                                                                                                                                                                  gtccTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCt                                                                                  <  1:220882/249‑1 (MQ=255)                                                                                                                                                                     cTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGC‑ACGTAGCCCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTCTTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGGTTTGCCATGAGCTCCGGTCCGCTGGTTTGGGGTCTGTGATCCGTAATTCAGCCGCTGa                                                                                >  2:292164/1‑250 (MQ=255)                                                                                                                                                                      tGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGaa                                                                               >  1:124683/1‑250 (MQ=255)                                                                                                                                                                        aCCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCGCTCTCCGTCGGCGCAGTATTTCGCCAGCGCCATGCTGCGGATGTTTATTGTCGTTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAACTTAGCCGCTGAAAgg                                                                            <  2:60750/251‑1 (MQ=255)                                                                                                                                                                           aaCGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCcg                                                                         >  2:234701/1‑251 (MQ=255)                                                                                                                                                                            aCGTACTGGCCACCATTATCGCAATCGGCCTTGTTGACCGCGGGGG‑ACGGAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCGGGCATTGGCGTACTCGTTATAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATGTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGGTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCcgc                                                                        <  1:213149/251‑1 (MQ=255)                                                                                                                                                                              gTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAg                                                                                                                               >  1:370874/1‑194 (MQ=255)                                                                                                                                                                              gTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAg                                                                                                                               <  2:370874/194‑1 (MQ=255)                                                                                                                                                                                 cTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAACCCAACGCTACCGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGTTGTTTCTTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGGTTTTGGTACTGTGCTCCGCAATTCAGCCGCTGAACGGCCGCGAttt                                                                   >  2:239947/1‑251 (MQ=255)                                                                                                                                                                                   tGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGAtttt                                                                  <  1:12868/250‑1 (MQ=255)                                                                                                                                                                                     ccACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCTCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCTTTGAAAGGCCGCGATTTTGGc                                                               <  2:105617/251‑1 (MQ=255)                                                                                                                                                                                                 cAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGCTGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGCTTTTGGCATCAACTGCTcc                                                   >  2:274857/1‑251 (MQ=255)                                                                                                                                                                                                 cAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTcc                                                   <  1:266067/251‑1 (MQ=255)                                                                                                                                                                                                   aTCGGCCTTTGTGACCGCTGTGG‑ACGTAAACCAACGCTATCGCTTGGATTCCTGGCGATGGGTTCTGGCATGGGCGTACTCGGTACATTGATTCAGATCGTTATTCACTCTCCGTCGGTGCAGGATTTCGCCATCGCCAGGCTGATGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGGCCGCTGATTTGGGTACTTTGCTCCGAAATTCAGCCGATGAAAGGCCGCGATTTTGGCATCCCCTGcaccac                                                 <  2:60774/251‑1 (MQ=255)                                                                                                                                                                                                       gCCTTGTTGACCGCTTGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCGGGCATGGGCGTACTCGGTACAATGCTGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCAGGAGTGCCGGTCCGCTGATTTGTGTACTTTGCTCCGAAATTCAGCCGCTGAAAGGCCTCGATTTTTGCAGCACCTGCTCCCCTGcc                                             <  2:383561/251‑1 (MQ=255)                                                                                                                                                                                                           tgttgACCGCGGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATTCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGccac                                           <  1:300804/249‑1 (MQ=255)                                                                                                                                                                                                                        gg‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGAtttccc                             >  2:247614/1‑246 (MQ=255)                                                                                                                                                                                                                           aCGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAAcc                          >  2:5945/1‑250 (MQ=255)                                                                                                                                                                                                                                aaccatcgctacgactGGGCGTCCCGGCGATGGCGGCTGGCATGGGCGTACTCGGTCCAATGAGGCATATCGGTATTCTCTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGAGGTCTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCCCCACTGCCTCCAACTGGATTGCCAACATGATc                     <  2:15432/237‑1 (MQ=255)                                                                                                                                                                                                                                       gctaacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCACCTGGATTGCCAACATGATCGGTGgcg              >  2:232236/1‑251 (MQ=255)                                                                                                                                                                                                                                          aacgatGGGATTCCTGGCGATGGCTGCTGGCATGGGCGTAATCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCTATGCTGCAGATGTTTATTGTCGGTTTTGCTATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGCAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCa            >  2:255067/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTCTTGTCGGTTTTGCACTGAGTGCCGGTCCGCTGATTTGGGTACTGTGATCCGAAATTCAGCCGCTGAAATGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGTTTGCCAACATGATCGTTGGCGCACCGTTCCTgc  >  2:371367/1‑250 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACG‑TCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTAT‑ACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGG‑ACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGA  >  NC_000913/3089013‑3089502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑