Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,861	G→A	V354I (GTC→ATC)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,861	0	G	A	100.0%	232.7 / NA	66	V354I (GTC→ATC)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/30);  total (36/30)

CGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGG  >  NC_000913/3065628‑3066105                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     cGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCTGCCTGGAGTTTGat                                                                                                                                                                                                                                     <  1:357487/251‑1 (MQ=255)        tcctccGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTAc                                                                                                                                                                                                                               <  1:153117/250‑1 (MQ=255)                            cGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAacacac                                                                                                                                                                                                          >  2:344990/1‑251 (MQ=255)                             ggAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTACCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACg                                                                                                                                                                                                         >  2:265629/1‑251 (MQ=255)                             ggAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTACAGCCTGACACTCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAAATCAACACACg                                                                                                                                                                                                         >  2:89819/1‑251 (MQ=255)                               aaGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGt                                                                                                                                                                                                        >  1:6854/1‑250 (MQ=255)                                              cGGTGGTGCATTTGCTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGTCAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCtt                                                                                                                                                                                        <  2:4562/251‑2 (MQ=255)                                              cGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACCCGTAGCCGGGGTCGATCtg                                                                                                                                                                                        >  1:425913/1‑251 (MQ=255)                                               ggtggtGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCtg                                                                                                                                                                                        >  1:176116/1‑250 (MQ=255)                                                     gCATTTACTGGAAACCGGCAAAATCAGCGGGGCAAGCGCCTCCAGCCTGACAATCTCGGCCGCTTCCCTGCGCAAGATTTACTACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAAc                                                                                                                                                                                  <  2:167822/250‑1 (MQ=255)                                                               gAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATcggcgg                                                                                                                                                                       >  2:326702/1‑251 (MQ=255)                                                                    cGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGCACATCGGCCTGGCGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCg                                                                                                                                                                  >  1:29640/1‑251 (MQ=255)                                                                    cGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCg                                                                                                                                                                  <  1:227755/251‑1 (MQ=255)                                                                          aaTCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCACGATTTACGACAATTTGGCTTACTTTGCCCGCAGCATTGTGTTGCGTCCGCCGGCGGTTTCCAATAACCCGGCAATCCTCCGTCGTCTGGGCGTAATCGTTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCAGGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCCGCGGGGAtt                                                                                                                                                            >  2:423816/1‑251 (MQ=255)                                                                           aTCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACACTATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCACTAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGCTCTGATGAACGGCATCGGCGGCCGCGGTGAttt                                                                                                                                                           >  1:396432/1‑251 (MQ=255)                                                                              aGCGGGTCCAGCGCCTACAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGACAGCCGCATTGAGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGCTGAACGGCATCGGCGGCAGCGGGGATTTTgc                                                                                                                                                        >  1:402580/1‑250 (MQ=255)                                                                                cGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCCGCGGTGAtttttaa                                                                                                                                                       >  2:228281/1‑247 (MQ=255)                                                                                   ggCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCAGGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGaacgca                                                                                                                                                   <  1:305859/251‑1 (MQ=255)                                                                                    gCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGGGAGTTTGaacgca                                                                                                                                                   >  2:74880/1‑250 (MQ=255)                                                                                     ccAGCGCCTCCAGCCTGACAAGCGCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGAGTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCGGGGCGTCATCGCTCTGAACATCGGCCTTGAGTTTGATATTTACGGGCATGCCAACTCAATACACGTAGCCGGGGTCGATCTGATGAACGGCAGCGGCGGCAGCGGTGATTTTGaacgcaac                                                                                                                                                 <  1:197235/251‑1 (MQ=255)                                                                                      cAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGaacgcaacg                                                                                                                                                <  1:6327/251‑1 (MQ=255)                                                                                           ccTCCAGCCTGACAATCTCGGCCTTTTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGGGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTaa                                                                                                                                           <  2:6556/251‑2 (MQ=255)                                                                                                aGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTc                                                                                                                                      >  1:364315/1‑251 (MQ=255)                                                                                                   cTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATACCCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGAt                                                                                                                                   >  1:413106/1‑251 (MQ=255)                                                                                                             cGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTAt                                                                                                                             <  2:146498/247‑1 (MQ=255)                                                                                                               gCCGTTTCCCTTCGCAAGATTTACGACAATATGGATTACGTTGCCAGCCGCATTGTGTTGCGTCCTCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCTTATCTGTCGATCTTTATGGcccc                                                                                                                       <  2:20418/251‑1 (MQ=255)                                                                                                                       cctggggaaGAATTACGACAATAAGGATTACTTTGCCAGCCTCATTGGGGTGCGGCCGCAGGAGATGTCCAATAACCCGGAAATCATCCGGCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGAGATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACTCGTATCTGTCGAGCTTTATGGCCCCGTCGATTg                                                                                                               <  2:342279/244‑1 (MQ=255)                                                                                                                       ccTGCGCAAGATTTACGACAATATGGATTACTGTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTg                                                                                                               <  2:6854/251‑1 (MQ=255)                                                                                                                               aGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCTGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGGCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGaacgcaacg                                                                                                                                                <  1:274856/210‑1 (MQ=255)                                                                                                                                 aTTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGTGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGCACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAgg                                                                                                     >  1:459918/1‑251 (MQ=255)                                                                                                                                 aTTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAgg                                                                                                     >  2:195718/1‑251 (MQ=255)                                                                                                                                 aTTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGGCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAgg                                                                                                     <  1:379910/251‑1 (MQ=255)                                                                                                                                      cGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGAGTTTGAACGCAACGCGGATCTGTCGATCTTTATGGCCCCGGCGATTGCTAAAGAAGGCAAGa                                                                                                <  2:212162/251‑1 (MQ=255)                                                                                                                                        aCAATATGGATTAATTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCa                                                                                                   >  2:273691/1‑246 (MQ=255)                                                                                                                                               ggagtACTTTGCCAGCAGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCCACACACGTAGCCGGGGTCGATCTGATGAACGGTATCGGAGGCATCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAAcc                                                                                        <  1:369579/246‑1 (MQ=255)                                                                                                                                                   tACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGcccc                                                                                                                       >  2:179897/1‑215 (MQ=255)                                                                                                                                                   tACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGcccc                                                                                                                       <  1:179897/215‑1 (MQ=255)                                                                                                                                                           cAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCCCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAAtgg                                                                           >  1:102997/1‑250 (MQ=255)                                                                                                                                                             gCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGc                                                                         >  1:103079/1‑251 (MQ=255)                                                                                                                                                             gCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGc                                                                         <  1:119879/251‑1 (MQ=255)                                                                                                                                                                  aTTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCCATGTGCAGcc                                                                     >  1:88343/1‑250 (MQ=255)                                                                                                                                                                  aTTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGcc                                                                     >  1:175213/1‑250 (MQ=255)                                                                                                                                                                  aTTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTAAGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGcc                                                                     >  1:74116/1‑250 (MQ=255)                                                                                                                                                                    tgtgTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCAt                                                                   <  1:74880/250‑1 (MQ=255)                                                                                                                                                                                 cAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATAGGCCTGGCGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATAGGAGGCAGCGGTGATTTTGAACGCAAAGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCCAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCg                                                      >  1:66124/1‑250 (MQ=255)                                                                                                                                                                                   ggAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAac                                                   <  2:364315/251‑1 (MQ=255)                                                                                                                                                                                     agaTTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGAGGGGATTTTGAACGCAACGCGTATCTGACGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGCGCCAATGTGCAGCCATGTTGATCCCAGCGAacac                                                 >  2:177972/1‑251 (MQ=255)                                                                                                                                                                                           ccAATAACCCGTAAATCAGCCGTCGTCGGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGTCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAGTGTGCAGCCATTTTGATCACAGCGAACACAGCGta                                           <  2:277112/251‑2 (MQ=255)                                                                                                                                                                                            cAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCa                                          <  1:175222/251‑1 (MQ=255)                                                                                                                                                                                                aaCCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAg                                       <  1:273691/250‑1 (MQ=255)                                                                                                                                                                                                  cccGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGGGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTg                                     >  2:396192/1‑250 (MQ=255)                                                                                                                                                                                                           catcCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGGCAAAGTGATCATCACCg                           <  2:465019/251‑1 (MQ=255)                                                                                                                                                                                                            atcCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCg                           >  1:94548/1‑250 (MQ=255)                                                                                                                                                                                                              ccGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGaa                         <  1:344990/250‑1 (MQ=255)                                                                                                                                                                                                               cgtcgtCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCCGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCCATGTGCAGCCATGTTGATCACAGCGAACACATCGTCAAAGTGATCATCACCGAAc                        >  2:332472/1‑250 (MQ=255)                                                                                                                                                                                                                gtcgtcTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCCGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTTGTGCCAATGGGCAGCCATGTTGATCCAAGCGAACCCAGCGTCCAAGTGATCATCACCCAACa                       >  2:248328/1‑250 (MQ=255)                                                                                                                                                                                                                  cgtcTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAAATCTCAACCGTCGTGCCAATGTGCAGCCCTGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAgg                    >  2:203441/1‑251 (MQ=255)                                                                                                                                                                                                                       ggtcGTCATCGCTCTGAACATCGGCCGGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATc                <  2:29640/247‑1 (MQ=255)                                                                                                                                                                                                                          cGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAgg                                                                                                     >  2:33568/1‑162 (MQ=255)                                                                                                                                                                                                                          cGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAgg                                                                                                     <  1:33568/162‑1 (MQ=255)                                                                                                                                                                                                                          cGTCACCGCCCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCCCCTCAACAAACGTAGCCGGGGTCGATCTGCTGAACGGCATCGGCGGCCGCGGTGATTTTGAACGCAACGCGTATCTGTCGCTCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCATAGTGCCAATGTGCAGCCATGTTGATCACAGCGGACACAGCGTCAAAGCGATCATCACCGAACCAGGGATCGCCg            >  2:194220/1‑251 (MQ=255)                                                                                                                                                                                                                           gTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGAGATTTTGAACGCAACGCGTCTCTGTCGATCTTTATGGCCCCGTCGATAGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAgggcacgccg            >  1:65212/1‑243 (MQ=255)                                                                                                                                                                                                                            tCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGAt          >  1:182942/1‑251 (MQ=255)                                                                                                                                                                                                                               tCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTg       >  1:22764/1‑251 (MQ=255)                                                                                                                                                                                                                                cGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTgc      <  1:377824/251‑1 (MQ=255)                                                                                                                                                                                                                                      gAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCgg  <  1:265629/249‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     CGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATATGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGG  >  NC_000913/3065628‑3066105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑