Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,040,623	0	G	A	60.9%	17.6 / 73.4	64	noncoding (107/120 nt)	rrfA	5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (12/13);  new base A (16/23);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCATTGGCGCAGAAAAAAATGCCGG  >  NC_000913/4040375‑4040868                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      aGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTTAGGCGTAACCGTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATGTTCAGCCTGATACAGATTCAAGCAGAACGCATAAGCTGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGGCCCTCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAAc                                                                                                                                                                                                                                                    <  1:311732/251‑1 (MQ=11)    gATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTg                                                                                                                                                                                                                                                  >  1:337370/1‑251 (MQ=14)    gATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTg                                                                                                                                                                                                                                                  >  2:268831/1‑251 (MQ=14)       gCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGcc                                                                                                                                                                                                                                                <  1:370344/250‑1 (MQ=14)       gCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCa                                                                                                                                                                                                                                               <  1:43581/251‑1 (MQ=14)        cGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGGAACGCCGTAGCGCCGCTGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGcc                                                                                                                                                                                                                                                >  2:9580/1‑249 (MQ=14)              gCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGCTGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATc                                                                                                                                                                                                                                         >  2:319227/1‑250 (MQ=14)                        aCTAAAGAAACGTGAGGCTTAAACTTACCACGCCAAAGGTGGTTTGGCGGATTGAGAGAAGAGTTTCAGCCTGAGACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGACTGGCGGCAGTAGCGCGGTGGTCCCACCTGATCCCATGTCGATATCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCGCCTCAGGCGAGAGTAGGAAACTGCCAGGCAGCAAATaaaccg                                                                                                                                                                                                                               <  2:344510/250‑4 (MQ=11)                                 ccGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAg                                                                                                                                                                                                                     <  2:110670/251‑1 (MQ=25)                                     gAGGCTTAACCTTACAACGCCGAAGGTGTGTTGGCGGATTGAGAGAAGAGTTTCAGTCTGATTCAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCTGCAGGAGTGCGGTGGTCCCCCCTGACCCCATGCCGAAATCAGATGTGAAACGCCGTAGCGCCGATGGTAGTGTGGTGTCTCCGCATGCGATAGTAGGAAACGGCCAGGCATCAAAGAAAACGAACGGTTCAGTCgg                                                                                                                                                                                                                 <  2:323977/251‑1 (MQ=11)                                       ggCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa                                                                                                                                                                                                               <  1:320646/251‑1 (MQ=37)                                             aCCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTgg                                                                                                                                                                                                         <  1:295860/251‑1 (MQ=38)                                                    aaCGACGAAGGTGTTTTTGCGGATTGAGAGAAGATTTTCAGCCTGATACAGAGTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTTGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTTGGGGCTCCTCATGCGAGAGGAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGTAAGGCTGGGCCtcta                                                                                                                                                                                                  <  2:270970/251‑4 (MQ=11)                                                       gctgAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCTCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGGGCAGTCGGAAGACTGGGCCTTTCGtt                                                                                                                                                                                               <  2:412011/248‑1 (MQ=255)                                                              ctgtTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCtgt                                                                                                                                                                                       >  1:297578/2‑251 (MQ=11)                                                                     ggCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGttgtttg                                                                                                                                                                                 >  2:404832/1‑251 (MQ=25)                                                                     ggCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGttgttt                                                                                                                                                                                  <  1:139091/250‑1 (MQ=25)                                                                           ttGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATaaaa                                                                                                                                                                                                                                 <  2:143843/197‑1 (MQ=17)                                                                           ttGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATaaaa                                                                                                                                                                                                                                 >  1:143843/1‑197 (MQ=17)                                                                              agagaAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCGCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGGCGGTGAAc                                                                                                                                                                        <  2:177926/251‑1 (MQ=18)                                                                                agaagaTTTGCAGCCTGATACAGATTAAAGCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTTCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGAGGGTAGTGTGTGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGGTGGAAGACTGGGTCTTTCGTTTTACCTGATGTTTGTCGGTGAACGc                                                                                                                                                                      <  2:433456/251‑1 (MQ=11)                                                                                 gaagaTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGACACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGct                                                                                                                                                                     >  1:111213/1‑251 (MQ=18)                                                                                     aTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTcc                                                                                                                                                                 >  1:372120/1‑251 (MQ=33)                                                                                         tcatccTGATACAGAGTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCTATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGa                                                                                                                                                              <  1:298506/246‑1 (MQ=16)                                                                                         tCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTg                                                                                                                                                               >  1:87514/1‑249 (MQ=18)                                                                                           aGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACt                                                                                                                                                                                                           >  1:213626/1‑203 (MQ=18)                                                                                           aGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCGGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACt                                                                                                                                                                                                           <  2:213626/203‑1 (MQ=18)                                                                                                gATACAGATTAAATCAGAACGCAGAAGCGGTCTGAGAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTTGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCGCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTGTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACa                                                                                                                                                      <  2:370917/251‑1 (MQ=9)                                                                                                     aGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATcc                                                                                                                                                 <  1:321435/251‑1 (MQ=18)                                                                                                        ttAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATccgcc                                                                                                                                              >  1:77016/1‑251 (MQ=18)                                                                                                            aTCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGCCTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCgggg                                                                                                                                          >  2:150677/1‑250 (MQ=18)                                                                                                            aTCAGAACGCAGAAGCGGTCTGATAAAACAGAAGTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGa                                                                                                                                          <  1:217762/251‑1 (MQ=18)                                                                                                                      aGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTTGGCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTg                                                                                                                                                                           <  2:58278/208‑1 (MQ=18)                                                                                                                      aGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTg                                                                                                                                                                           >  1:58278/1‑208 (MQ=33)                                                                                                                      aGAAGCGGTCTGATAAAACAGAATTTGCCGGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAg                                                                                                                                                                                                                                              <  1:302672/141‑1 (MQ=17)                                                                                                                      aGAAGCAGTCTGATAAAACAGAATTTGCCTGGCGGAAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGCACTCAGAAGTGACACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAg                                                                                                                                                                                                                                              >  2:302672/1‑141 (MQ=11)                                                                                                                         aggggTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACg                                                                                                                             <  1:242540/248‑1 (MQ=18)                                                                                                                             gTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGcc                                                                                                                                                                                                      <  1:233732/174‑1 (MQ=21)                                                                                                                             gTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGGAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGc                                                                                                                         <  1:160694/251‑1 (MQ=18)                                                                                                                             gTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGcc                                                                                                                                                                                                      >  2:233732/1‑174 (MQ=21)                                                                                                                                 gATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGa                                                                                                                       <  1:370676/249‑1 (MQ=18)                                                                                                                                     aaaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGAAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc                                                                                                                 <  2:97018/251‑1 (MQ=18)                                                                                                                                       aCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACgg                                                                                                               >  2:292584/1‑251 (MQ=33)                                                                                                                                       aCAGAATTTGCCTGGCGGCAGAAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACgg                                                                                                               <  1:414189/251‑1 (MQ=18)                                                                                                                                           aaTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc                                                                                                                 <  2:360213/245‑1 (MQ=33)                                                                                                                                           aaTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc                                                                                                                 >  1:360213/1‑245 (MQ=33)                                                                                                                                                    ggcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGc                                                                                                  <  2:263139/251‑1 (MQ=33)                                                                                                                                                    ggcggcAGTAGCGCGGAGGTCCCACCTGACCCAATGACGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCACATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGCCAAATCCGCCGGGAGCGGATTTGAACGTTGCGCAGCAACGGCCCGGAGGGTGGc                                                                                                  >  2:162227/1‑251 (MQ=11)                                                                                                                                                     gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCAGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCg                                                                                                 <  1:250069/251‑1 (MQ=18)                                                                                                                                                          aGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCaa                                                                                                                  >  1:133240/1‑229 (MQ=33)                                                                                                                                                          aGTAGCGCGGTGGGCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCaa                                                                                                                  <  2:133240/229‑1 (MQ=18)                                                                                                                                                           gTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAgg                                                                                           <  2:232712/251‑1 (MQ=33)                                                                                                                                                             aGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAc                                                                                         <  2:358575/251‑1 (MQ=33)                                                                                                                                                                          cACCTGACCCCATGCCGCACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAAc                                                                            >  1:127641/1‑251 (MQ=18)                                                                                                                                                                                  cccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCtgtt                                                                                                                                                                                      <  1:275848/137‑1 (MQ=37)                                                                                                                                                                                  cccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCtgtt                                                                                                                                                                                      >  2:275848/1‑137 (MQ=37)                                                                                                                                                                                                  aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGCTTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGa                                                     >  2:322868/1‑250 (MQ=18)                                                                                                                                                                                                      gAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAACGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGCCGCCCGCCCTAAACTGCCAGGCATCAACTTAAGCAGAAGGcc                                                >  1:214304/1‑251 (MQ=16)                                                                                                                                                                                                                     cGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATACGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCCTAACATGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGc                                 >  2:212744/1‑251 (MQ=16)                                                                                                                                                                                                                       aTGGTAGTGTGGGGTCTCCTCATGCGCGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc                                                                                                                 >  2:209977/1‑169 (MQ=25)                                                                                                                                                                                                                       aTGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc                                                                                                                 <  1:209977/169‑1 (MQ=35)                                                                                                                                                                                                                         ggTAGTGTGGGGTCCCCTCAGGGGAGAGAAGGAAACTGCCAGGAATAAAATAAAACGAAAGGCTCATTCGGAAGAGTGGGCTTTTGGTTTTATCTGTTGTTTGTGGGTGAACGCTCCCCTGAGTAGGACAAACCCGCGGGGGGCGAATTTGAACGTTGCGAAGCACCGGCCGGGAGGGTGGCGGGAAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCttt                             <  1:268831/251‑1 (MQ=11)                                                                                                                                                                                                                              tgtgGAGTCTCCTCATGCGAGAGTAGGAAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCTGAGGGTGGCGGGCAGGAAGCCCGCCATAAACGGCCCGGCCTCAAATTACGCAGAAGGCCATCCCGACGGATGGCCTTTTTGcc                        >  2:7473/1‑250 (MQ=11)                                                                                                                                                                                                                                                     tAGGAAACTGCCAGGCATCAAATAAAACGAAACGATCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCCTAAACTGCCAGGCATCAAATTAAGCAGAAAGCCCTCCCGCCGGAATGCCTTTTTGCCTTGTAGCAGAAAAAACTGcccg  >  2:290116/1‑248 (MQ=11)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      AGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCATTGGCGCAGAAAAAAATGCCGG  >  NC_000913/4040375‑4040868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑