| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 377,432 | 0 | C | G | 20.6% | 80.8 / 17.7 | 34 | intergenic (+121/+103) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB |
| Reads supporting (aligned to +/- strand): ref base C (12/15); new base G (2/5); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAACG‑GATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGT > NC_000913/377183‑377681 | aGCGTTGCCGATAAAATTGCTCGGCGTGATGACAGCAGTATAGGCTGGCACCGCGCGCTCAGCGACGAATAGCAGGATGAAGACGACCCGGAAGCCGAATTCAAAGTGCCTGATGATTTGATGTGGTAAACAGAGAGAGGTCGTGCGCAGATGCCTGATGCGACCCTATGCGCGTCTTATGAGGCCTACGGTTTATGGGGGAAGTGTAGACCGGAGAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑Ca < 2:43334/251‑1 (MQ=25) gATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCGGAAGAGCAGGATGAAGACGTCCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGAGGCCGGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATg < 2:278807/251‑1 (MQ=255) aaaTTGCTCAGCGCGATTCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGa < 1:190558/251‑1 (MQ=255) cgcgATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGt > 1:164153/1‑251 (MQ=255) gtTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCt‑t > 1:456999/1‑250 (MQ=255) acaGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGTCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacg < 1:200717/251‑12 (MQ=25) cgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaacc > 1:117021/1‑234 (MQ=21) cgcCGAAGAGCAGGATGAAGACGACCCGGATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGTGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccgtaggcc < 1:288870/251‑25 (MQ=18) ccgcagcccAGGATTACGACGAGCCGCATGCCGACTTCAACGTCCCTGATGATTAGATGTGGTAAACATAAATAAGTCGTGCGCAGCTGCCTGATGCGCCGCCATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGACGTGTAGACCGGATCAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGAAGCTTTGCGCGTCTTATCAGGCCTATG‑‑GCT‑TACGGGCGACGTGTAGGCCggc > 2:46381/9‑250 (MQ=37) cGAAGAGCAGGATGAAGACTACCCGTAGGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGAGGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTAGGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGAt < 1:450191/251‑1 (MQ=255) cGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcg < 1:52860/237‑14 (MQ=21) cGAAGAGCAGGATGAAGACGACACGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTCCGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcg > 2:52860/1‑224 (MQ=21) acCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CGTGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATcc < 1:423105/251‑1 (MQ=255) gACTTCAAAGGGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCgacctgaaaa < 1:261075/251‑10 (MQ=2) aCTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCt < 1:10838/251‑1 (MQ=255) agttcccgatgatTTGCTGTGTTAAACATAGATATGTCGTGCGCAGCGGCCTGATGCGACGCTATGCGCGGTTTACCAGGTCTTCGGTTTATGGGCGAAGTGCAGTCCGGATAAGGCGTTCACGCCGCATCCGTCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGACTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGTATAAGGCGTTCACGCCGCAGCCGGCAGTCGTGCTATTATCa < 1:166639/244‑1 (MQ=255) cagaaaatTTGATGTGGTAAACAAAGATAATTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTTTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGGTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCa < 2:197806/245‑1 (MQ=255) tgGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTAttt > 2:112543/1‑250 (MQ=255) atagaataGTCGTGCGCAGGTGCCGGATGCGACGTGAGGGGGGTCTTATCGGGCCTACGGTTTATGGGCGAAGTGTGGCCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGAGGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTa < 2:381509/244‑1 (MQ=37) tAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGt > 1:341252/1‑249 (MQ=255) gCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATaaa < 1:270429/251‑1 (MQ=255) gcgTCTTATCAGGCCTACGTTTTAGGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCTTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCTGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGTTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAg < 1:285915/251‑1 (MQ=255) tataaGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCtgtg < 2:176081/246‑1 (MQ=255) aGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCAt > 1:256062/1‑251 (MQ=255) ggcgtACGGTTTATGGGCGAAGTGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGGGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATa < 2:156032/247‑1 (MQ=255) cGGTTTATGGGCGAAGTGTAGCCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGTGCGA‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGACGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGGAGGCAATATGATCGCCAATAAAAATGGA‑GACCAAATAATTGCTGAGATCATAACCCTc > 2:459668/1‑251 (MQ=255) ggTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGACGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACAGGA‑GACAAAATAATAGCTGTGATCATAACCCTCg > 2:439331/1‑251 (MQ=255) ttATGGGGGAAGGGTAGACCGGAAAAGGCGTTCACGCCGCATCCGTCAGTCGTGCGC‑CATGCCTGAGGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTg < 2:243970/250‑1 (MQ=255) gggCGAAGTGTATACCGGATAAGGCGTTCACGCCGCAGCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGTCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAAc < 2:256062/251‑1 (MQ=255) tgtAGACCGGAAAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAAAGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCACCAATAAAATTTGA‑GACAAAATAATAACTGTGATCATATACCTCGTGATAACG‑GGTTAAc > 2:326028/1‑251 (MQ=37) aGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAACG‑GATTAACGTc > 1:270293/1‑251 (MQ=255) gTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTCGTGATATTATCAACGCATATTCAGTTTATTAGCGGTGTGAGCAATATGATCGCCAATAAAAATGGAAGACATA‑TAATACCAGTTATTAAAACCCTCGTTATAACGTGAT‑AACGTCTTgcaattttactc > 2:120576/1‑240 (MQ=21) ccgcATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAACG‑GATTAACGTCTTGATATTCATCTCCTGGc > 1:14584/1‑249 (MQ=255) cAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGTCGTTCACGCCGCATCCGGCAGGCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAACG‑GATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCaa < 1:282271/251‑1 (MQ=255) c‑cATGCCTGATACGACGCTTTGCGCGTTTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGGCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAACG‑GATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGt < 2:341252/251‑1 (MQ=255) | AGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGA‑GACAAAATAATAGCTGTGATCATAACCCTCGTGATAACG‑GATTAACGTCTTGATATTCATCTCCTGGCAGATCTTTTCAAGATTTGGAGT > NC_000913/377183‑377681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |