Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913377,4020GA32.6% 53.3 / 31.0 44intergenic (+91/+133)yaiL/frmBDUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
Reads supporting (aligned to +/- strand):  ref base G (13/16);  new base A (6/8);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTC  >  NC_000913/377162‑377640
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
gAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTAcgcc                                                                                                                                                                                                                                          >  1:18568/1‑251 (MQ=255)
    aCGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCAt                                                                                                                                                                                                                                      <  1:281808/251‑1 (MQ=255)
        cATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                                                                                                                  >  1:243970/1‑251 (MQ=255)
         attattACCGCCAGCGTTGCCGATAAAATTGATCAGAGCGATGCGAGCAGTAGAGAGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGAACCGTATGCCGGCTTCAAAGTGCCTGATGATTTGATGTGGAAAACATAGATAAGACGAGCGCAAATGCCGGATGCTACGCTATGCGCGTATTATCAGGCCTACGGTTTATGGGCGCAGTGTAGACCGGATCAGCAGTTTCCGCCGCATCCGGc                                                                                                                                                                                                                                 >  1:429180/1‑251 (MQ=255)
            attCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGt                                                                                                                                                                                                                              >  1:381509/1‑251 (MQ=255)
            attCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTAGAGTGTTGCCCAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATACGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGt                                                                                                                                                                                                                              <  1:260033/251‑1 (MQ=255)
              tCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGGGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCattt                                                                                                                                                                                                                             <  1:298316/250‑4 (MQ=255)
                 cGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTTATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTAGGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCatttgtgc                                                                                                                                                                                                                         <  1:269423/251‑8 (MQ=255)
                  gCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg                                                                                                                                                                                                                        >  1:454358/1‑251 (MQ=255)
                   ccAGCGCTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGACGTGTAGACCGGATAAGAAGTTTACGCCGCATCCGGCATTTGTgcgc                                                                                                                                                                                                                       >  2:56724/1‑251 (MQ=255)
                     aGCGTTGCCGATAAAATTGCTCGGCGTGATGACAGCAGTATAGGCTGGCACCGCGCGCTCAGCGACGAATAGCAGGATGAAGACGACCCGGAAGCCGAATTCAAAGTGCCTGATGATTTGATGTGGTAAACAGAGAGAGGTCGTGCGCAGATGCCTGATGCGACCCTATGCGCGTCTTATGAGGCCTACGGTTTATGGGGGAAGTGTAGACCGGAGAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑Ca                                                                                                                                                                                                                    <  2:43334/251‑1 (MQ=25)
                             cGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGATCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcgctgatgcctg                                                                                                                                                                                                              >  1:252240/1‑241 (MQ=255)
                              gATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCGGAAGAGCAGGATGAAGACGTCCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGAGGCCGGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATg                                                                                                                                                                                                           <  2:278807/251‑1 (MQ=255)
                                  aaaTTGCTCAGCGCGATTCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGa                                                                                                                                                                                                        <  1:190558/251‑1 (MQ=255)
                                             cgcgATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGt                                                                                                                                                                                            >  1:164153/1‑251 (MQ=255)
                                                                 gtTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCt‑t                                                                                                                                                                      >  1:456999/1‑250 (MQ=255)
                                                                      acaGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGTCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacg                                                                                                                                                                   <  1:200717/251‑12 (MQ=25)
                                                                            cgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaacc                                                                                                                                                             >  1:117021/1‑234 (MQ=21)
                                                                                   cgcCGAAGAGCAGGATGAAGACGACCCGGATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGTGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcgaaccgtaggcc                                                                                                                                                      <  1:288870/251‑25 (MQ=18)
                                                                                     ccgcagcccAGGATTACGACGAGCCGCATGCCGACTTCAACGTCCCTGATGATTAGATGTGGTAAACATAAATAAGTCGTGCGCAGCTGCCTGATGCGCCGCCATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGACGTGTAGACCGGATCAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGAAGCTTTGCGCGTCTTATCAGGCCTATG‑‑GCT‑TACGGGCGACGTGTAGGCCggc                                                                                                                                                 >  2:46381/9‑250 (MQ=37)
                                                                                      cGAAGAGCAGGATGAAGACTACCCGTAGGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGAGGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTAGGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGAt                                                                                                                                                <  1:450191/251‑1 (MQ=255)
                                                                                      cGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcg                                                                                                                                                                 <  1:52860/237‑14 (MQ=21)
                                                                                      cGAAGAGCAGGATGAAGACGACACGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTCCGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTAcaaatctgtacgcg                                                                                                                                                                 >  2:52860/1‑224 (MQ=21)
                                                                                                    aaGACGACCCGTATGCCTACTTCAAAGTGCCTGATGAGTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCAcgcc                                                                                                                                                                                                                                          <  1:185970/151‑1 (MQ=255)
                                                                                                    aaGACGACCCGTATGCCGACTTCCAAGTGCCTGATGATTTGATGTGGTAAACATAGATAGGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCAcgcc                                                                                                                                                                                                                                          >  2:185970/1‑151 (MQ=255)
                                                                                                          acCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CGTGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                            <  1:423105/251‑1 (MQ=255)
                                                                                                                     gACTTCAAAGGGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTG‑ACGCGTCTTATCATGCCTACAAATCTGTACG‑‑CGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCgacctgaaaa                                                                                                                  <  1:261075/251‑10 (MQ=2)
                                                                                                                      aCTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCt                                                                                                                <  1:10838/251‑1 (MQ=255)
                                                                                                                             agttcccgatgatTTGCTGTGTTAAACATAGATATGTCGTGCGCAGCGGCCTGATGCGACGCTATGCGCGGTTTACCAGGTCTTCGGTTTATGGGCGAAGTGCAGTCCGGATAAGGCGTTCACGCCGCATCCGTCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGACTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGTATAAGGCGTTCACGCCGCAGCCGGCAGTCGTGCTATTATCa                                                                                                         <  1:166639/244‑1 (MQ=255)
                                                                                                                                  cagaaaatTTGATGTGGTAAACAAAGATAATTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTTTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGGTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCa                                                                                                    <  2:197806/245‑1 (MQ=255)
                                                                                                                                                tgGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTAttt                                                                                      >  2:112543/1‑250 (MQ=255)
                                                                                                                                                        atagaataGTCGTGCGCAGGTGCCGGATGCGACGTGAGGGGGGTCTTATCGGGCCTACGGTTTATGGGCGAAGTGTGGCCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGAGGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTa                                                                              <  2:381509/244‑1 (MQ=37)
                                                                                                                                                         tAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGt                                                                               >  1:341252/1‑249 (MQ=255)
                                                                                                                                                                             gCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATaaa                                                         <  1:270429/251‑1 (MQ=255)
                                                                                                                                                                                                gcgTCTTATCAGGCCTACGTTTTAGGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCTTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCTGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGTTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAg                                      <  1:285915/251‑1 (MQ=255)
                                                                                                                                                                                                      tataaGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCtgtg                                 <  2:176081/246‑1 (MQ=255)
                                                                                                                                                                                                          aGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCAt                            >  1:256062/1‑251 (MQ=255)
                                                                                                                                                                                                           ggcgtACGGTTTATGGGCGAAGTGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGGGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATa                           <  2:156032/247‑1 (MQ=255)
                                                                                                                                                                                                                 cGGTTTATGGGCGAAGTGTAGCCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGTGCGA‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGACGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGGAGGCAATATGATCGCCAATAAAAATGGAGACCAAATAATTGCTGAGATCATAACCCTc                     >  2:459668/1‑251 (MQ=255)
                                                                                                                                                                                                                  ggTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGACGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACAGGAGACAAAATAATAGCTGTGATCATAACCCTCg                    >  2:439331/1‑251 (MQ=255)
                                                                                                                                                                                                                     ttATGGGGGAAGGGTAGACCGGAAAAGGCGTTCACGCCGCATCCGTCAGTCGTGCGC‑CATGCCTGAGGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTg                  <  2:243970/250‑1 (MQ=255)
                                                                                                                                                                                                                         gggCGAAGTGTATACCGGATAAGGCGTTCACGCCGCAGCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGTCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAAc             <  2:256062/251‑1 (MQ=255)
                                                                                                                                                                                                                                 tgtAGACCGGAAAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAAAGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCACCAATAAAATTTGAGACAAAATAATAACTGTGATCATATACCTCGTGATAACGGGTTAAc     >  2:326028/1‑251 (MQ=37)
                                                                                                                                                                                                                                    aGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTc  >  1:270293/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
GAATACGCCATTATTCCCGCCAGCGTTGCCGATAAAATTGCTCAGCGCGATGCCAGCAGTATAGTGTTGCACAGCGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGC‑CATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACG‑‑GCT‑TACGGGCGAAGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCTATTATCAACGCATATTCAGTTTATTGGCGTGGTAGGCAATATGCTCGCCAATAAAACTGGAGACAAAATAATAGCTGTGATCATAACCCTCGTGATAACGGATTAACGTC  >  NC_000913/377162‑377640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑