| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 354,288 | 0 | T | G | 30.8% | 119.9 / 13.3 | 66 | V528G (GTG→GGG) | prpE | propionyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (7/38); new base G (20/0); total (28/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTG > NC_000913/354043‑354535 | gccgccGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGaa < 1:331560/249‑1 (MQ=255) ccgGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATgg > 1:433877/1‑250 (MQ=255) tgtATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGaagatggggtga > 1:464442/1‑240 (MQ=255) gtATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGGGCTgga > 1:190529/1‑248 (MQ=255) tATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCGTCCGTGACGCTGACGGTTATAACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGAGCGAGATTGAAGAGAGTATCACCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGG‑GAGAGATGTGCTggt > 1:449009/1‑247 (MQ=255) tCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCAGCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCGCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAggg < 1:51603/250‑1 (MQ=255) ggggCGACGACGGCCGCTTTGAGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGCGTACGCCCCCTTTGACTGGGGCCTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAAATgcgcagggaggggggggggggggg > 2:205835/1‑231 (MQ=255) cgacgGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGGGCTG‑GAAGGGG‑AGgtgggggtggcggttt > 1:219168/1‑247 (MQ=255) ggCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCAATTTATTCTCGAGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAGATGGGCTGGAAAggggggtgggggggggggttggtat > 1:193952/1‑229 (MQ=255) ggCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACGGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCAt < 2:464442/251‑1 (MQ=255) ttGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGaa < 1:34366/250‑1 (MQ=255) tggaagacttatcgggcgcttgtttCCCGCCCGGTGTACGGCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAag < 2:17546/228‑1 (MQ=255) aGACTTACTGGTCGCTTTTTTCCCGCCCGGTGTTCGCCACCTTTGACTGGGGTATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTTCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGTGGTGGTCATTCCGAAAGAGAGc < 1:318235/251‑1 (MQ=255) acgtacgggTCGCTGTTTTCCCGCCCGGTGGACGCCGCCTGTGAGTGGGGCATCCGTGACGCTGACGGGTAGCACTGTATTCGCGGGCGCTCTGACGATGGAATTAACGTTGCCGGGCATCTGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTT‑AAAGGTC‑AGGTGGCGGTGGTGTTTGTCATTCCGAAaggtagaga < 2:440095/244‑8 (MQ=255) aCTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGa < 1:34196/251‑1 (MQ=255) tACTTGTCGCGGTTGTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCTATCCGGGCGGTGCCGAAGGGGCGGTGG‑TTGGGGTGAAAGATGCGCTT‑AAAGGGC‑AGGTGGCGGTGGCGTGTGTCATTCCGAAAGAGAGCGAc < 2:433877/249‑1 (MQ=255) tACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGTGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTT‑AAAGTGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGATAGCGACa < 2:428295/250‑1 (MQ=255) ggTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTc < 1:205835/249‑1 (MQ=255) cGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAGAAGCGCTG‑AAAGGGC‑CGGGGGCGGGGGCGGTTGTCATTCCGAAAAAGAGCGACC‑GTCTGGa > 1:323700/1‑250 (MQ=255) gTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTGTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGa < 1:125667/249‑1 (MQ=255) ccgGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCCGCCATCCGGGCGTTGCCGCAGTGGCGGAGG‑GTGGGGGGAGAGATGAGGTG‑AAAGGGC‑AGGTGGCGGGGGGGGTTGTCCTTCCGAAAAAGAGCGACA‑GTCTGGGAGATCGTgcggtggg > 2:178975/1‑244 (MQ=255) ggTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAAAAGCGCTG‑AAAGGGC‑AGGGGGGGGGGGGGGTTGTCATTTCGAAAAAGAGCGGCA‑GACTGGAAGATCGGGATGTGgcgg > 1:13528/1‑250 (MQ=255) gtgtACGCCTCCGTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTGGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGGTTGGCATTCCTAAAGAGAGCGACA‑GTCTGGAAGAGCGTGATGTGGCGCa < 2:419613/251‑1 (MQ=255) gtacgcctctggtggcgggggCATGAGTGACGCTGACGGTTAGGACTGGAGTCTCGGGCGCACTGAGGATGGAAGGAACGTTGCCGGGGAGCGGCTGGGGACGCGCGAGATTGAAGAGAGTATAGCCAGCCAGCAGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGAGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCAca < 2:121402/234‑2 (MQ=255) tGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg < 1:172031/250‑1 (MQ=255) gACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc < 2:9780/251‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑t < 1:338466/250‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTACTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑tt < 1:410784/251‑1 (MQ=255) cggtGACGGTTATCACTTTATGCTCGGGCGCACTGACGATGTAATTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATGCCGAAAGAGAGCGACA‑TTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCtggtgg < 2:231006/248‑1 (MQ=255) cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGGGGCGGTTTTCATTCCGCAAGAGAGCGACA‑GGCTGGGAGATCGGGATGTGGCGCACTCGCCAGAGAAGGCGA‑TTATGGCGCtggggggc > 1:284125/1‑246 (MQ=255) tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAAATGGGCTG‑GAAGGGG‑CGGGGGCGGGGGGGGTTGGCATTCCCAAAGAGGGGGGCA‑GGCTGGGAGATCGGGATGGGGCGCCCCCGCAAGGGAAAGCGG‑TTATTGCGCtggggtgg > 2:103496/1‑245 (MQ=255) ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGATGGGGCG‑AAAGGGGGAGG‑GGGGGGGGGGGTTGTCAATCCCAAAGAGAGAGACA‑GTCTGGGAGATCGTGATGGGGGGCACCCGCAAGAGAAGGGGA‑GTATAGCGCtggggggtagcc > 1:409672/1‑239 (MQ=255) tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAGGTGCGCTG‑AAAGGGG‑AGGGGGCGGGGGCGGTTTTCATTCCGAGAGAGAGGGGCA‑GGCTGGGAGAACGGGATGGGGGGCCCCCCCCAGAGGAGGCGA‑GTATGGCGCTGGTGGACAACCAgcgtg > 2:402132/1‑247 (MQ=255) gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGgccg < 1:169654/251‑1 (MQ=255) gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCg < 1:308044/251‑1 (MQ=255) cACTGACGATGTAATGAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCgg < 1:246818/251‑1 (MQ=255) gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCg < 1:368543/246‑1 (MQ=255) gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGgcg < 1:178975/249‑1 (MQ=255) cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAAATGCGGCA‑AAAGGGT‑CGGGGGGGGGGGGGTTTGTCATTCCGGAAGAGAGGGACAAGT‑GGGAAGATCGTGAAGGGGGGCACTAGAAAGAGAAAGGGG‑GTATGGCAGTGGGGGGCAGGAAGAATGGCAAC‑ATAGGACGCCCGGCGCACg > 1:449472/1‑251 (MQ=255) gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCt < 2:323700/250‑1 (MQ=255) tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGGGGAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGG‑TTATGGCGCTGGTGGGCAGCCAGATTGGGCAC‑TTTGGCCGCCCGGCGCCCGGCTgg > 1:157915/1‑251 (MQ=255) tAACGGTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGttt < 2:284125/250‑1 (MQ=255) gtggCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGtctc < 2:317678/248‑1 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAAtt < 1:216186/251‑1 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAAtt < 1:103496/251‑1 (MQ=255) gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGGAAGGTGGGCTGGAAGGGGG‑GGGGGGGGGGGGGGTTTGTC‑TTCCGAAAGAGAGGGGCA‑GACTGGGAGATAGTGGGGTGGGGCACTCGCGAGAGAAGGGGG‑GTAGTGCGGGGGGGGGCAGCCCGATTGGCAAC‑TTTGGGCGGCCGGGGCCCGTCTgggttttttcggaatt > 2:151665/1‑237 (MQ=255) cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGGGTTTGTCATTCCGAAAGAGAGCGACC‑GTCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAAGCGA‑TTATGGGGCTGGGGGACAGCCAGATTGGGAAC‑TTTGGCCGCCCGGCGCCCGGCTGGTTTGTCTCGCAAtttcc > 1:360382/1‑248 (MQ=255) cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAGAGAAGCGCTG‑AAAGGGC‑AGGGGGGGGGGGCGGTTGTCATTTCGGAAGAGGGCGACA‑GGCTGGGAGATTGTGGTGTGGCGGCCTCGCCAGAGAAGGCGG‑TTATGGCGCTGGGGGGCAGCCCGATTGGCAAA‑TTTGGGCGCCCGGCGCCCGGATGGGTTGTTTCGCAATTGCCaa > 1:395447/1‑250 (MQ=255) cgcgAGATTGCAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTCG‑TTGGGGTGAAAGATACGGCG‑AAAGGGC‑AGGGGGCGGGGGCGTTTGTCATTTCGAAAGAGGGCGACA‑GACTGGAAGATCGGGGAGTGGCGCCCTCGCACGGGGAGGCGA‑TTATGGCGCTGGTGGAGAACCCGCGTGGGCAC‑TTTTGCCGCCCGGGGCGCCGCTGGTTTGTTTCGCAAATTCCAAAAAAGCGATAC‑gg > 1:65430/1‑251 (MQ=255) gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGTGG‑TTGGGGGGAAAGATGCGCTG‑AAAGGGC‑AGGGGGGGGGGGGGGTTGTCATTTCGAAAAAGAGCGAAA‑GTCTGGAAGGACGTGATGTGGCGCACTCGCCAGAGAAGGGGA‑TTATGGCGCTGGGGGACAGCCAGATTGGGAAC‑TTTTGACGCCCGGCGCGCGGCTGGTTTGTCTCggcatttacaaaaaagcgataccgaa > 1:280650/1‑225 (MQ=255) gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAAATGCGCCG‑AAAGGGC‑AGGGGGGGGGGGCGCTTGTCCTTTCGAAAAAGAGCGGCC‑GTCTGGAAGGACGGGGTTTGGGGCACTCGCAAAAGAAAGCGAGTTTTGG‑GCTGGGGGGCCGCCAGAGTGGGAACCTTGGGCCCCCCGG‑GCCCGGCTGGTTTTTCTCGCCATTTCCCAAAACACGATCCCGGaaa > 2:241103/1‑250 (MQ=255) aTTGAAGAGAGTATTCCCAGCCATACGAGCGGTGACGGAGTGGCGGTGT‑TTGGGGTGACAGATGCGATT‑AAAGGGC‑AGGTGGCGGGGGCGTATGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCTCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACATCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTGTGGTTTGTCTCCCAATTGCCAAAAACGCGATCC‑TGAAAAAt < 2:61694/251‑1 (MQ=255) ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGAt < 1:388663/250‑1 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑GTGGGGGGAAAGATGCGCTG‑GAAGGGG‑CGGTGGCGGGGGCGGTTGTCCTTTCGCAAGAGAGGGACA‑GTCTGGAAAATCGTGGTGTGGGGGACTCGCCAGAAAAGGCGA‑TTATTGCGCTGGTGGAAAGCCAGAATGGCAAC‑CTTTGCCCCCCGGCGCACGTCTGGTTTGTCTCGCCATTGACAAAAACGCGATTC‑GGGAAAATGCTGcgccgcccgctccc > 2:155880/1‑243 (MQ=255) gCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAg < 1:115926/251‑1 (MQ=255) ccATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTTATGTGGCGCACTCGCATGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCTGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCatg < 2:229597/251‑3 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGa < 2:101598/250‑1 (MQ=255) cGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGAtt < 1:138385/251‑1 (MQ=255) gggCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGAttt < 1:402132/251‑1 (MQ=255) cGTTGCCGAAGTGGCGGTGG‑TTGTGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCg < 1:245817/251‑1 (MQ=255) ggCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATc > 1:260315/1‑251 (MQ=255) cGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCt > 1:199032/1‑251 (MQ=255) gtgg‑tTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTTGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTgg < 1:445483/251‑1 (MQ=255) g‑tTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCCCGATCCAGGCGATTTGCGAAGGACGCGATCCTgg > 2:289556/1‑248 (MQ=255) gggTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTg > 1:155653/1‑251 (MQ=255) ggTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGCGATCTg > 1:397787/1‑250 (MQ=255) | GCCGCCGGGGTGTATTCAGACCATCTGGGGCGACGACGGCCGCTTTGTGAAGACTTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGG‑TTGGGGTGAAAGATGCGCTG‑AAAGGGC‑AGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACA‑GTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGA‑TTATGGCGCTGGTGGACAGCCAGATTGGCAAC‑TTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCC‑GGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTG > NC_000913/354043‑354535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |