Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,348,862	IS5 (+) +4 bp	intergenic (‑118/+450)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3652036 =	NA (NA)	40 (0.900)	34/484	0.3	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	4348862 =	0 (0.000)					intergenic (‑118/+453)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
*	?	NC_000913	= 3653230	NA (NA)	52 (1.170)	49/484	0.1	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 4348865	0 (0.000)					intergenic (‑121/+450)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

AATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/3651792‑3652279                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aaTTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTGCGTTCTAACCTGGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTTACGTAAATTTAGGGAAg                                                                                                                                                                                                                                                 <  2:309325/249‑1 (MQ=255)    tttATTGCTGAACAACGAGCTGACTTACCAACTAATATAACAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGCCGCAAATTTAGGGAAGGTGCg                                                                                                                                                                                                                                            >  2:77000/1‑251 (MQ=255)          gCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGACGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATa                                                                                                                                                                                                                                        >  2:163960/1‑249 (MQ=255)                 aactagcagACTTAACAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGCAGTTATATATTGAGCACAGTGAAAATAAAAAAAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCAATGCCGCAAATTTAGGGAAGGTGCg                                                                                                                                                                                                                                            >  2:257600/9‑238 (MQ=255)                 aacGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGct                                                                                                                                                                                                                                            <  1:257600/238‑2 (MQ=255)                         gACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATtcttct                                                                                                                                                                                                                       >  1:250649/1‑251 (MQ=255)                                 aaCTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAGCCAACGTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTAGTTCGAAGATCTACTTAAGATAAATTTCGCTGACGCAAATGTAGGGAAGGTGCTAAGAAGCGGGGAAAGTCTTCTCGGCTGAc                                                                                                                                                                                                               <  2:146704/251‑1 (MQ=255)                                       tataAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGAACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAAACAAATTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGATGACTCAGtc                                                                                                                                                                                                         >  1:277905/1‑251 (MQ=255)                                                           tGTATAAAACAATGACGAAGTTATATCTTGAGCACAGTGCAAATAACAACAGGCTGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTACCCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAACTTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGtttt                                                                                                                                                                                     >  2:203202/1‑250 (MQ=38)                                                                          cGAAGTTATGTATTGAGCTCAGTGAAAATCAAAACAGGCTGAAAGTCTTTGCCGGTACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAACTTTCGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTtctc                                                                                                                                                                      >  2:140208/1‑251 (MQ=38)                                                                                tatataTTGAGCACAGTGAAAATAAAAACAGGATGACAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGTTTGAGCCGATTTTTTCTCCCGTa                                                                                                                                                                 >  2:140982/1‑250 (MQ=255)                                                                                              aGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                                                                                                                                  <  1:268924/251‑1 (MQ=255)                                                                                                   caaTAAAAACCGGATGAAAGTCTTTGCCGTAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGACGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAt                                                                                                                                             >  2:245332/2‑251 (MQ=255)                                                                                                   aaaTAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAt                                                                                                                                             >  2:444875/1‑251 (MQ=255)                                                                                                           aCAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCg                                                                                                                                     >  1:367327/1‑251 (MQ=255)                                                                                                              ggatgcaagcatatGCCGGACCCAACTTTATTGATTTCAATATGACGGAACAGCACCTGTCCGGGTTCGTTCTACCCTTGTCAAGGTTATATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCAGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGttt                                                                                                                                  >  2:433158/14‑251 (MQ=21)                                                                                                                              cGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc                                                                                                                  <  1:17990/251‑1 (MQ=255)                                                                                                                                    cAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc                                                                                                            >  2:43912/1‑251 (MQ=255)                                                                                                                                              gATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGttttt                                                                                                  <  2:367327/251‑1 (MQ=255)                                                                                                                                                tttCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGCCGTTATCCCCAGttttt                                                                                                  >  2:365997/1‑249 (MQ=255)                                                                                                                                                   cAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                              >  1:376954/1‑250 (MQ=255)                                                                                                                                                    aaTATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                              >  1:335781/1‑249 (MQ=255)                                                                                                                                                        tGACAGGACAGAACCTGTCCGGGTTCGTTCTAACATTGTAAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCCGCCTATTTAGCCCGTTTCTTCGCCATTTAAGGCGTTATCCCAAGTTTTTAGTGAGAtct                                                                                        >  2:73170/1‑251 (MQ=255)                                                                                                                                                               acagAACCTGTCCGGGTTCGTTCTAACCTTGTCCAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTATTATCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGACTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCAc                                                                                 >  2:276456/1‑251 (MQ=255)                                                                                                                                                                cagAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACt                                                                                >  2:141931/1‑251 (MQ=255)                                                                                                                                                                   aaCCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAACTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGAc                                                                             >  1:24196/1‑251 (MQ=255)                                                                                                                                                                    aacTGTCCGGGTTCTTTCTAACCGTGGCAAAGTTTAAAGTCGAAGATCTGCGTAAAATAAATTTAACTGGCACAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCGTCTCGGCTGACGCAGTCATTTCAGTTCCCCATGATAGAGCCGATTGTTTCGCCCGTAAATGCCATAAAGCTTCCGATTTAGACCGCGTCTTCGGCATTGAAGGCGTTACGCCCAGTTGTTAGTGAGATCTCTCCCACttgcg                                                                            <  2:293638/249‑5 (MQ=9)                                                                                                                                                                     ccTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGt                                                                                                                <  1:210841/214‑1 (MQ=255)                                                                                                                                                                     ccTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGt                                                                                                                >  2:210841/1‑214 (MQ=255)                                                                                                                                                                            gggTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATACATTTCACTGACGCAAATTTCGGGCAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCAttt                                                                    >  2:190184/1‑251 (MQ=255)                                                                                                                                                                                 cgttcTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTc                                                                <  1:419068/250‑1 (MQ=255)                                                                                                                                                                                       aaCCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATGTCTTCATGTTTGAGCCGATTTTTTCGCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAGTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCg                                                          <  1:301812/250‑1 (MQ=255)                                                                                                                                                                                                aaGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTg                                                >  1:328974/1‑251 (MQ=255)                                                                                                                                                                                                  ggTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGc                                              >  1:271258/1‑251 (MQ=255)                                                                                                                                                                                                      ttATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAg                                           <  2:114146/250‑1 (MQ=255)                                                                                                                                                                                                       tATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGc                                          <  1:444875/250‑1 (MQ=255)                                                                                                                                                                                                            cGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAAt                                    <  2:376954/251‑1 (MQ=255)                                                                                                                                                                                                            cGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAAt                                    >  1:309053/1‑251 (MQ=255)                                                                                                                                                                                                             gAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATa                                   <  1:77000/251‑1 (MQ=255)                                                                                                                                                                                                               aGATCTAATTAATATAAATTTCACTGACGAACATTTAGGGAAGGTGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCCTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCACGTAAATGCCTTGAATCAGCATATTTAGACCGTTTCTTCGACATTTAAAGCGTTATCACCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGATCCGCCTGACACAGGTTGGCCAGCGTGAATAAc                                 >  2:285575/1‑251 (MQ=14)                                                                                                                                                                                                                        tAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGt                        >  1:360507/1‑251 (MQ=40)                                                                                                                                                                                                                                        gACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTATTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCGGTTTTTAGTGAGATCTCTCCCACTGACGTATCCTTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTc         >  1:240955/1‑250 (MQ=17)                                                                                                                                                                                                                                        gACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCGTCTCGGCTTACTCAGTCATTTCATTTCGTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAGCCCCAGTTTTTAGTGAGATCTCGCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGGTGGTTAGCGTTTTTc         <  2:274006/250‑1 (MQ=11)                                                                                                                                                                                                                                              aaTTTAGGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGAAAcc  >  1:368033/1‑251 (MQ=11)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AATTTTATTGCTGAACAACGAGCTGACTTACCAACTAATATAAAAGATATTCAAAGCCTGTATCAAAAAATGACGAAGTTATATATTGAGCACAGTGAAAATAAAAACAGGATGAAAGTCTTTGCCGGAACCAACTTTATTGATTTCAATATGACAGGACAGAACCTGTCCGGGTTCGTTCTAACCTTGTCAAGGTTTTATTTCGAAGATCTACTTAATATAAATTTCACTGACGCAAATTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/3651792‑3652279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.