Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	458,790	IS186 (–) +6 bp :: Δ1 bp	intergenic (+90/‑93)	clpX → / → lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	458790 =	0 (0.000)	35 (0.800)	34/478	0.3	100%	intergenic (+90/‑98)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
	?	NC_000913	608008 =	NA (NA)					noncoding (2/1345 nt)	IS186	repeat region
*	?	NC_000913	= 458795	1 (0.020)	24 (0.550)	23/476	0.6	95.9%	intergenic (+95/‑93)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
	?	NC_000913	= 609351	NA (NA)					noncoding (1345/1345 nt)	IS186	repeat region

GTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGAAGACGTCGAAAAAGTGGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACAtccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/458551‑458795 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGGATTATGTCGTCAATTA  <  NC_000913/609351‑609107                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            GTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGTAAGACGTCGAAAAAGTGGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCAT                                                                                                                                                                                                                                                 <  2:439380/251‑1                      ACCATGTACGATCTGCCGTCCATGGAAGACGTCGAAAAAGTTGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGA                                                                                                                                                                                                                            <  1:449886/251‑1                             ACGATCTGCCGTCCATGGAAGACGTCGAAAAAGTGGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCT                                                                                                                                                                                                                     <  1:134923/251‑1                                    gaCGTCCATGGAATACGTCGAAAAAGGGGGTATCGACGAGTCGGGAATTGAATGTCAAAGCAAACCGTTGCTGATGTAAGGCAAGCCGGAAGCGCAACAGGAATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCCCTATTCTCGGCTTTGAATGTGGGGGAAACATCTCATAAGCGCTACCTTAAGGGTTGAACCATCTTAAGAAT                                                                                                                                                                                                              <  2:367241/249‑1                                       GTCCATGGAAGACGTCGAAAAAGTGGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCG                                                                                                                                                                                                           >  2:117238/1‑251                                                               GGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACG                                                                                                                                                                                   <  2:375393/251‑1                                                                       ACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCG                                                                                                                                                                           <  1:439389/251‑1                                                                               gtcATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATACTTCACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTCTTCTCGGCGTTGAATGTGGGGGACACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGCCGCTGCCTCGCGTTCTTC                                                                                                                                                                   >  2:448231/4‑251                                                                                                     CGTTGCTGATTTATGGCAAGCCGGAAGCGCAACATGCATCTTGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAGCTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCGCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCA                                                                                                                                             <  1:272081/251‑1                                                                                                            GATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACCATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATAC                                                                                                                                      >  2:289535/1‑251                                                                                                            GATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGAT                                                                                                                                        <  1:123322/249‑1                                                                                                                                    ACAGGCATCTGGTGAATAATTAACCATGCCCATACAATTATTTAACCAAAAAGGGGGGATTTTAGCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCTACGCCTCGGTGCCTCTTTAAGACGATGCCTCGCGTTCTTCAAGTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAA                                                                                                              <  1:441993/251‑1                                                                                                                                      AGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAACAGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGAGAACCCTCCCATAAGCGCTAACTTAAGGGTTGCACCATCTGAAGAATGCGACGCCTCATGGCCTCGTTCAGCAGATGCCTCGCGTTCTTCACTTGCGTTTTGTAGGCTGTCAGGGCTACTGTCCCACGAATGGCCACCTGTA                                                                                                               >  1:308539/1‑248                                                                                                                                                            caaTCCCATACAATTAGTTAACCAAAAAGGGGGTATTTTATCTCCCCTTTAATTTTTCCTCTAGTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGCTAT                                                                                      <  1:272748/248‑1                                                                                                                                                                               AACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTAGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGACTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGATTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCCCCTGTAAGCTCCAGATGACCATTTTTGATATTCTCAACAACGAGTTAGTT                                                                   >  1:407852/1‑251                                                                                                                                                                                       AGGGGAGATTTTACCTCCCCTTTAATTTTTCCTCTATTATCGGCGTTGAATGTGCGCGAAACATCCTATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGCGCCTCGTTAAGACGCTGCCTCGCGCTCTTCAATTGCGATTTGTAGGCTGTCAGGGATACTGTCCCACGAATGCCCACCTGTAAGCTCCAGATGACCATTTTTGTTATCCTCCACAACGACTTAGTTCTTCTTTT                                                           >  2:217784/1‑251                                                                                                                                                                                                        CCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTATGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCCCGAATGGCCACCTGTAAGCTCCAGATGAACATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTG                                          >  2:142559/1‑251                                                                                                                                                                                                                  TTTCCGCTATTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCACCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCGGGGGGGGAAA                                 <  1:142559/250‑1                                                                                                                                                                                                                   TTCCTATATTCTCGGCCTAGAATGTGGGGGAAACATCCCATAAGAGCTAACTTAAGGGTTGAACCATATGAAGAATGCGACGCCTCGGTGCATCGTTAAGACGATGCCTCGCGTTATTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGCTGACCATTTTTGTTATTCTCCACAAAGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATC                               >  2:180319/1‑251                                                                                                                                                                                                                           TTCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATG                       >  1:416182/1‑251                                                                                                                                                                                                                            TCTCGGCGTTGAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTGGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAGGACGATGCCTCGCGTTCTTCAAGTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCAGGAATGGCCACCGGTAAG                                                                                                             <  1:308032/164‑1                                                                                                                                                                                                                            TCTCGGAGTTGAATGTGGGGGCAACATCCCATAAGCGCTCACTTAAGGGTTGAACCCTCTGCAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCCATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAG                                                                                                             >  2:308032/1‑164                                                                                                                                                                                                                                      GAATGTGGGGGAAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGGATTATG            <  1:448231/251‑1                                                                                                                                                                                                                                                 AAACATCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGGATTATGTCGTCAATTA  >  2:251066/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            GTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGAAGACGTCGAAAAAGTGGTTATCGACGAGTCGGTAATTGATGGTCAAAGCAAACCGTTGCTGATTTATGGCAAGCCGGAAGCGCAACAGGCATCTGGTGAATAATTAACCATTCCCATACAATTAGTTAACCAAAAAGGGGGGATTTTATCTCCCCTTTAATTTTTCCTCTATTCTCGGCGTTGAATGTGGGGGAAACAtccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/458551‑458795 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGGATTATGTCGTCAATTA  <  NC_000913/609351‑609107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.