Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,454	Δ1 bp	coding (1273/1632 nt)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,454	0	A	.	100.0%	106.3 / NA	24	coding (1273/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/12);  total (12/12)

ACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAGGATTCGGCCATTGTTCCTGT  >  NC_000913/1302211‑1302704                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                           acTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTc                                                                                                                                                                                                                                                    <  1:371667/251‑1 (MQ=255)                              gACTCTGCCAGAATGGGTTAGCTGGAGCCAGCACACCAGCAACGAAGAGGCGAAACAACTGCTGGCTGCCGCGGGTTATACCGCAGACCAACCGTTGACAATCAACCTGTTGCATAACACCTCAGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGCGGCAGCAAAACATTGGTGTAACCGTCAAACTGGATACCCAGGAGTGGAAA‑CGTTCATCGCCACCAGTCACCAgcgaccttctgatgt                                                                                                                                                                                                                       >  2:34238/1‑237 (MQ=255)                                                tGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtg                                                                                                                                                                                                     >  1:165716/1‑251 (MQ=255)                                                                        cGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtc                                                                                                                                                                             <  1:184758/251‑1 (MQ=255)                                                                                    aaaaCTGCTGGCTGAAGCGGGTGATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCAGAAAAAGCTGGCGATTGCTGCGTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCGCCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAacac                                                                                                                                                                 <  1:227211/251‑1 (MQ=255)                                                                                                  aaGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACTCATCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGATTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGGACTTGTGATGTGGCCCGTGCAGGCTGGTGTGCTGATTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACa                                                                                                                                                   <  1:289261/251‑1 (MQ=255)                                                                                                     cGGGTTATACCGCAGACAAACCGTTGCCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGGGTGCTGGCTACAACGAACAAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                                                                                                                >  2:66076/1‑251 (MQ=255)                                                                                                     cGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                                                                                                                <  2:303953/251‑1 (MQ=255)                                                                                                          tataCCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGAt                                                                                                                                            <  2:182827/250‑1 (MQ=255)                                                                                                                       aaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCgcgcctt                                                                                                                              >  2:376544/1‑248 (MQ=255)                                                                                                                                ccATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc                                                                                                                     >  2:67325/1‑251 (MQ=255)                                                                                                                                     aaCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTc                                                                                                                                                                                                                                                    <  2:53393/119‑1 (MQ=255)                                                                                                                                     aaCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTc                                                                                                                                                                                                                                                    >  1:53393/1‑119 (MQ=255)                                                                                                                                      aCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGCTTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACAAGGCGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCt                                                                                                               >  2:20949/1‑251 (MQ=255)                                                                                                                                            tgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGAc                                                                                                          <  1:376544/250‑1 (MQ=255)                                                                                                                                                           aTCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGCGTGGCAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGACCCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGGATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaa                                                                                           >  2:41060/1‑250 (MQ=255)                                                                                                                                                                 aTAAACAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAACACATAGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaggcgctg                                                                                     >  2:69786/1‑243 (MQ=255)                                                                                                                                                                   aaaaaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTTAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGaaa                                                                                  <  1:329787/251‑1 (MQ=255)                                                                                                                                                                                   ctgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAAACAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGATGAAAGTGACTGCAGAGgcgc                                                                  >  2:212955/1‑251 (MQ=255)                                                                                                                                                                                     gcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTACTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACGACAACGAACCAACTGCCTTCCTGAACACCATGCTTTCGAACAGCTCTATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGgcgca                                                                 <  2:383465/250‑1 (MQ=255)                                                                                                                                                                                               tgtgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAA‑GTGACTGACGAGGCGCAGCGCACAGctc                                                      <  1:41060/250‑1 (MQ=255)                                                                                                                                                                                                         aaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTa                                             >  1:226118/1‑250 (MQ=255)                                                                                                                                                                                                                         gTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGAGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTg                            >  1:393969/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAg                      <  2:165716/249‑1 (MQ=255)                                                                                                                                                                                                                                                    aCGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATACGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGAGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATGAGGATTCGGCCATTGTTCCTGt  >  1:223227/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                           ACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAGGATTCGGCCATTGTTCCTGT  >  NC_000913/1302211‑1302704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑