| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,911,929 | 2 bp→TT | coding (647‑648/882 nt) | htpX ← | protease HtpX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,911,929 | 0 | C | T | 96.2% | 79.6 / NA | 26 | W216* (TGG→TAA) | htpX | protease HtpX |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (16/9); minor base A (1/0); total (17/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,911,930 | 0 | C | T | 100.0% | 84.9 / NA | 26 | W216* (TGG→TAA) | htpX | protease HtpX |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/9); total (17/9) | |||||||||||
ACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTGACCATATCACCATTGGCGATGTGGCTGATTTC > NC_000913/1911697‑1912159 || aCTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGTCCTGAACCACCCACTGAGCGATTTCGACTTTCCGTTAATGCAGAGAGCC‑CTCATGCTGGTTGCTTTTTGCGGTTCATTGCTTGGTTTCAGGCGCTGCCGCGCGGCAATTCTTTTCTCGCGGCCAACCCGTTTTTCCGCCCCCGGATCAGCATGGGATTTACCTTGACCCGAGAAATACATTGTGATAATggcc > 2:6182/1‑248 (MQ=255) cACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGGGGGGCATTAACAACTCACTTAGCGGTTTCCACTTACCGTTAATGCAAGGAGCCCTTCA‑GATGGGTGCTTATTTCGGTTCATAGCTGGGTTTCAGGCGCTGCCACGGGGCAATAATTTTCTCCGGACCCAACAGTTTTAACGCACCGGCATCAGCATGGAATTTCCGCTGACGCGACAATTACATGGGGTTAATGCTCgaccaaatacc > 1:439334/1‑240 (MQ=255) tCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGtt < 2:156480/250‑1 (MQ=255) ggAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTg < 2:51519/250‑1 (MQ=255) cTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGctctc > 2:202644/1‑251 (MQ=255) cGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTTCGGTTCATAGCTGGTGTTCAGGCGCTGCAGCGCGGCAATCATTGTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCt < 1:427263/168‑1 (MQ=255) cGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGATGCTTATTGCGGATCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGCCCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCt > 2:427263/1‑168 (MQ=255) aCTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTtcatc > 2:94010/1‑250 (MQ=255) aCTTACCGATAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTCCATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTtcatcc > 2:162646/1‑250 (MQ=255) tGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGccc > 2:42638/1‑251 (MQ=255) tCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGCATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTACCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACggg > 1:218874/1‑250 (MQ=255) cATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGa < 2:136536/250‑1 (MQ=255) gCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATa > 1:318804/1‑250 (MQ=255) tttCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGACTACCAAACACCAGTTcc > 2:378099/1‑133 (MQ=255) tttCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTcc < 1:378099/133‑1 (MQ=255) ggCAATCATTTTCTCGCGACCAACAAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGCTTTCCGCACATAACACCCGCGGCTAGCTGCGCCAGAATACGGGACATAAAGGTAACGAAGGGGATCACCACGc > 1:401566/1‑251 (MQ=255) aaTCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGCATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGCACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGCATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCt > 2:65414/1‑251 (MQ=255) tttCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATc < 2:107934/250‑1 (MQ=255) cgcgACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTc < 1:248954/251‑1 (MQ=255) aaccaGTTTTGCCGCACCAGCATCAGCATGGACTTCACGACGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCACACCCCCGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTg > 2:82379/1‑250 (MQ=255) aGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGACGGTTTTCACCACGCCCTGACTCAGCGTCATGGTTACCat > 2:19325/1‑251 (MQ=255) cAGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTGACCATATCACCATTGGCGATGTgg > 1:146596/1‑251 (MQ=255) aGCATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTGACCATATCACCATTGGCGATGTGGc > 2:136646/1‑251 (MQ=255) cATGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAAGCAGCGTCATGGTGACCATATCACCATTGGCGATGTGGCTg < 2:251923/251‑1 (MQ=255) aTGGAATTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTGACCATATCACCATTGGCGATGTGGCTg > 1:319853/1‑250 (MQ=255) aaTTCACGATGACGCGAGAATTACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTGACCATATCACCATTGGCGATGTGGCTGATTTc < 1:94010/251‑1 (MQ=255) || ACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACCGTTAATGCAGAGAGCC‑ATCATGCTGGTTGCTTCTTGCGGTTCATAGCTGGTTTTCAGGCGCTGCAGCGCGGCAATCATTTTCTCGCGACCAACCAGTTTTGCCGAACCGGCATCAGCATGGAATTCACGATGACGCGAGAACCACATGGTGATAATGCTCGCCAGAATACCAAACACCAGTTCCAGAACCGTTGCAACCGCAAAGTAGATCAGCGGGTTGCCGTTGCTCTCTTCACCTTCATCACGATTTCCGCCCATAAAACCCGCGGCAAGCTGCGCCAGAATACGGGAAATAAAGATAACGAAGGTGTTCACCACGCCCTGAATCAGCGTCATGGTGACCATATCACCATTGGCGATGTGGCTGATTTC > NC_000913/1911697‑1912159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |