Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,818,749 2 bp→AC intergenic (+165/‑124) dinD → / → yicG DNA damage‑inducible protein D/PF03458 family inner membrane protein YicG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,818,7490CA95.7% 72.7 / ‑5.4 23intergenic (+165/‑125)dinD/yicGDNA damage‑inducible protein D/PF03458 family inner membrane protein YicG
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (16/6);  total (17/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
*NC_0009133,818,7500TC95.7% 73.1 / ‑6.1 23intergenic (+166/‑124)dinD/yicGDNA damage‑inducible protein D/PF03458 family inner membrane protein YicG
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (16/6);  total (17/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTC‑‑CCCCTCAGTTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑CTGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCCCTCCCCTTAAAAAATCTCAATCGTGACAATGCGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCAC  >  NC_000913/3818532‑3818989
                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                               
aGGTCAGCATCAAGCAGTTGTAACACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGATACCCTATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTa                                                                                                                                                                                                                   <  1:211951/251‑1 (MQ=255)
          cAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGATACCCGATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCa                                                                                                                                                                                                         <  1:100846/251‑1 (MQ=255)
                                                       gAAACTAAAAACTCTTTTTGTTGATTGAGATACCCGATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGcaca                                                                                                                                                             >  2:18705/1‑250 (MQ=255)
                                                             aaaaaCTCTTTTTGTTGATTGAGATACCCGATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTaaaaa                                                                                                                                                                                     >  1:324271/1‑220 (MQ=255)
                                                             aaaaaCTCTTTTTGTTGATTGAGATACCCGATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTaaaaa                                                                                                                                                                                     <  2:324271/220‑1 (MQ=255)
                                                                       tttGTTGATTGAGATACCCGATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCCCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCca                                                                                                                                             <  2:22960/250‑1 (MQ=255)
                                                                                         cGATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACATGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGgag                                                                                                                            >  2:52327/1‑249 (MQ=255)
                                                                                          gATGCGTAAGCCAAAGTCCAGGTGCGGTGAACACAATGGCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGgag                                                                                                                            >  1:177315/1‑248 (MQ=255)
                                                                                                                               ggCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTtcct                                                                                                                                                                                                  <  1:232014/141‑2 (MQ=255)
                                                                                                                               ggCTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTtcct                                                                                                                                                                                                  >  2:232014/1‑140 (MQ=255)
                                                                                                                                 cTAAACATTGCACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACt                                                                                  >  1:238330/1‑251 (MQ=255)
                                                                                                                                           cACCTTTCTTTTCCACCCCTCAATTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACACATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGcc                                                                         >  1:109437/1‑250 (MQ=255)
                                                                                                                                                                   tttAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCgggc                                                >  1:120856/1‑249 (MQ=255)
                                                                                                                                                                    ttAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCgggcg                                               >  2:379568/1‑248 (MQ=255)
                                                                                                                                                                      aaCCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAggg                                                                                                                              >  1:44181/1‑170 (MQ=255)
                                                                                                                                                                      aaCCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGAGTTTTAggg                                                                                                                              <  2:44181/170‑1 (MQ=255)
                                                                                                                                                                              ttttCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATg                                     >  2:232032/1‑251 (MQ=255)
                                                                                                                                                                                 tCTTATGCATTTTCTCAGAAAGGAAGACCGAAAAATGCATCTACT‑CTACCGAACATAGCGATTTCCCCTAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACACATCGCTACCCTGCCAGACAGATTTTTAGGTAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGTCGGCCGGTCGTCGCCGCATGGAt                                  >  2:424412/1‑251 (MQ=255)
                                                                                                                                                                                   ttATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATAc                                >  2:219269/1‑251 (MQ=255)
                                                                                                                                                                                       gCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACAttt                            >  1:45289/1‑251 (MQ=255)
                                                                                                                                                                                                         agTCAGAAGAATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCg          >  2:126295/1‑251 (MQ=255)
                                                                                                                                                                                                             agaagaATGCATCT‑ACGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCTCTCACCTTAAAAAATCTCAATCGTGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCCGGGCGACGCCGCATGGATACATTTGGCGTAATTATTATTGCGACCg      >  1:88481/1‑251 (MQ=255)
                                                                                                                                                                                                                 gaaTGCATCT‑ACGCAACAGAAACTAGCGATTTCACATAACTACAAGATATCAATTTCCTCTCACCTTAAAAAATCTCAATCGAGACAATACGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCTCTGGCGGCAGGGCGACGCCGCATGGATACCTTTGGAGTGATTATTATTGCGACAGCCAc  >  2:383755/1‑251 (MQ=255)
                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                               
AGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTC‑‑CCCCTCAGTTTTAACCTATTTTTTCTTATGCATTTTCTCAGACAAGAAGTCAGAAGAATGCATCT‑CTGCTACAGAAAATAGCGATTTCACATAACTACAAGTTATCAATTTCCCCTCCCCTTAAAAAATCTCAATCGTGACAATGCGCACAAATCGCTACCCTGCCAGACAGATTTTTAGGGAGAGAACCATGCTGTTACACATTTTGTATTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTATTGCGACCGCCAC  >  NC_000913/3818532‑3818989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑