Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,817,287 2 bp→GT coding (612‑613/864 nt) yicC → putative RNase adaptor protein YicC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,2870AG97.3% 108.0 / NA 37E204E (GAA→GAG) yicCputative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (14/22);  minor base T (0/1);  total (14/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
*NC_0009133,817,2880CT100.0% 128.5 / NA 37L205L (CTG→TTG) yicCputative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/23);  total (14/23)

GCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  >  NC_000913/3817054‑3817513
                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                 
gCCGCTGAAATGCTCGCGGCGCTGGATGGTACGC‑GGAGCTCCTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCAGTGTTCGCGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCGCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGcaca                                                                                                                                                                                                                   <  2:99004/251‑1 (MQ=255)
      gAAATTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCCGCGTCTGGAAGGCGTAACCGCCGAAGTGGTCAAAGTCAGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAAAGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAAACGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAt                                                                                                                                                                                                             >  1:194897/1‑251 (MQ=255)
       aacgtctcGCTGGGCTGGATGGTACGCTGAA‑CGACTTTATGGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTTATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAtt                                                                                                                                                                                                            <  2:141893/247‑1 (MQ=255)
       aaaTTCTCGCGGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAACGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAACCAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAAtt                                                                                                                                                                                                            >  1:389470/1‑251 (MQ=255)
              cgcgGCGCTGGATGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGt                                                                                                                                                                                                       <  1:257778/249‑1 (MQ=255)
                cggcgCTGGATGGGACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGTATTTGGTTCTGCTGGCACAACGAATTGACGTTGcc                                                                                                                                                                                                   <  1:119670/251‑1 (MQ=255)
                 ggcgCTGGATGGTACGCTGAA‑CGACTTTAGTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCCGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCg                                                                                                                                                                                                  <  2:358158/251‑1 (MQ=255)
                         aTGGTACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCTGACACTGAAAGCATTGATCGAGCAGAGTCTGGAAGGCGTCACCGCCGACGTGGTCAAAGTCCGCTCCCCTATGCCGGCACTACTGCAATGGAAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCAGAAGAACTg                                                                                                                                                                                          >  1:293051/1‑251 (MQ=255)
                          ggttACGCTGAA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTgg                                                                                                                                                                                         <  1:412192/248‑1 (MQ=255)
                                gCTGAA‑CGACTGTATTGTCGCGCGCGAAACCGAAGGCCAGGCACTGAAAGCATTGATCGAGCAGCGGCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑GCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGcc                                                                                                                                                                                   <  2:361613/251‑1 (MQ=255)
                                    gaactacgttATTGTCGGGGGCGAAACCAAAGGTCGGGCACGGAAAGCATGGATCGATCAGCGGCTGAAAGTCGGCCCCGCCTAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGTGAAGCTGGAAGATGCGCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTTGTTCGGCTGGCACAACTAATGGACGTTGCCGAAGAACTGGGCCGCCTc                                                                                                                                                                                 <  2:275911/242‑1 (MQ=255)
                                           tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTg                                                                                                                                                                                                           <  2:48791/217‑1 (MQ=255)
                                           tttATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTg                                                                                                                                                                                                           >  1:48791/1‑217 (MQ=255)
                                             tATTGTCGCGGGCGAACCCGAAGGTCGGGCATTGAAAGCATTGACCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGGCCGCCTCGAAGCGCAt                                                                                                                                                                        <  2:52493/250‑1 (MQ=255)
                                                      gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAag                                                                                                                                                                 >  2:126338/1‑248 (MQ=255)
                                                       cgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTAACAGTCCGCTCCCATATGCCGGAAATCCTGCACTGGCAGCGTGAACGCTTGG‑TCGCGTAGCTGGAAGATGCTCCGGTTCAGTTGGACAACAACCGACTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTAGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGAc                                                                                                                                                             >  2:141969/1‑251 (MQ=255)
                                                           aaaCCGAAGGTCAGGCACTGAAAGAAGTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGGCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTac                                                                                                                                                         <  1:159869/251‑1 (MQ=255)
                                                                 aaGGTCAGGAACTGAAAGCATTGATCGTGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCAATATGCCGGAAATCCTGCTATGGCAGAGTGAACGCTTGA‑TAGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCAGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGACGAACTGGACCGCCTCGAATCGCATGTTAAAGAGTCGTACAACAtt                                                                                                                                                   >  2:2739/1‑251 (MQ=255)
                                                                              aaaaGCATTGATCGAGCAGCGCCTGGAAGGGGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACG‑CTGGGTCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTGGGTTCTGCTGGCACAACGAATTGACGGTGCCGAAGAACTGGACCGCCTCGAAGCGCATGGTAAAGAGACGTACAACATTCTGAAGAAAAaag                                                                                                                                      <  1:365611/250‑1 (MQ=255)
                                                                                 aGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAaaga                                                                                                                                     >  2:165763/1‑249 (MQ=255)
                                                                                       gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTg                                                                                                                             >  2:93456/1‑251 (MQ=255)
                                                                                       gATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGGACAACATTCTGAAGAAAAAAGAAGCCGTTg                                                                                                                             >  1:247497/1‑251 (MQ=255)
                                                                                        aTCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTgg                                                                                                                            >  2:430751/1‑251 (MQ=255)
                                                                                          cgatcagcGTCTGGAAGGCGTCCCCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGgtc                                                                                                                          <  2:364683/247‑1 (MQ=255)
                                                                                                       gAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACGGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGactt                                                                                                             <  1:101866/251‑4 (MQ=255)
                                                                                                        aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                                                                                                            >  1:412741/1‑251 (MQ=255)
                                                                                                        aaGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCGGCAATGGCAGCGTGACCGCTTGG‑TCGCGAGGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGGGTTGGTTCTGCTGGCACAACGAATGGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGActtt                                                                                                            <  2:225076/251‑1 (MQ=255)
                                                                                                         aGGCGTCACCGCCGAAGTGGTCAAAGGCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCGGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTa                                                                                                           <  1:21702/251‑1 (MQ=255)
                                                                                                               cACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTatgatg                                                                                                      <  1:83201/250‑1 (MQ=255)
                                                                                                                           ggTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAACGCTTGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAGTTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAAcc                                                                                         <  1:68698/251‑1 (MQ=255)
                                                                                                                                                                       gtgaacgcttGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTg                                             <  2:76756/242‑1 (MQ=255)
                                                                                                                                                                         gaacgcttGG‑TCGCGAAGCTGGAAGATGCTCAGGTTCAGTGGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGa                                            <  2:152784/243‑1 (MQ=255)
                                                                                                                                                                                             tGGAAGATGCTCAGGTTCAGTTGGAAAACAACAGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGAACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGcgattgagatg                        >  1:156833/1‑241 (MQ=255)
                                                                                                                                                                                                  gATGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTAGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAgt                   <  1:126338/251‑1 (MQ=255)
                                                                                                                                                                                                    tGCTCAGGTTCAGTTGGAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAgggt                 >  1:392033/1‑248 (MQ=255)
                                                                                                                                                                                                        caggttcatttGGAAAACAACCGTCGGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGttgat             <  1:314783/241‑1 (MQ=255)
                                                                                                                                                                                                                   ggAAAACAACCGTCTGGAGCAGGAGTTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGACCGCCTCGAAGCGCATGTTAAAGAGACGTACAACATTCTGAAGAAAAAAGAAGCCGTTGGTCGTCGTCTGGACTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCGATTGAGCTGAAAGTGTTGATTGAGCAGATgc  <  2:412741/251‑1 (MQ=255)
                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                 
GCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGA‑CGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGG‑TCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGGAGTTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGCCATCGAGCTGAAAGTGTTGATTGAGCAGATGC  >  NC_000913/3817054‑3817513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑